A deep learning strategy for accurate identification of purebred and hybrid pigs across SNP chips.

Background: Breed identification plays an important role in conserving indigenous breeds, managing genetic resources, and developing effective breeding strategies. However, researches on breed identification in livestock mainly focused on purebreds, and they yielded lower predict accuracy in hybrid. In this study, we presented a Multi-Layer Perceptron (MLP) model with multi-output regression framework specifically designed for genomic breed composition prediction of purebred and hybrid in pigs.

Neoadjuvant immunochemotherapy with adebrelimab combined with the TP regimen for locally advanced OSCC: early results of a single-arm phase II clinical trial.

Background: This study aimed to investigate the efficacy of a PD-L1 inhibitor combined with chemotherapy in the treatment of locally advanced OSCC.

Materials And Methods: This study was designed as a single-arm phase II trial, and a total of 30 patients were enrolled. All patients were pathologically confirmed to have head and neck squamous cell carcinoma.

A Co-catalytic nanosystem based on molybdenum disulfide and Prussian blue for synergistic chemodynamic and photothermal therapy through mitochondrial damage and ferroptosis.

Chemodynamic therapy (CDT) is regarded as an emerging strategy with high specificity for tumor therapy by producing highly toxic reactive oxygen species (ROS) in tumor cells by a Fenton or Fenton-like reaction. Excessive ROS can cause mitochondrial damage and induce ferroptosis in cells, leading to the death of cancer cells. However, the generally low efficiency of the Fenton reaction has limited the effectiveness of CDT.

Chemical Constituents of Anaphalis hancockii Maxim.

Twenty-seven compounds were isolated from Anaphalis hancockii Maxim in Qinghai Province, China, including 7 isobenzofuranones (1-7), 8 flavonoids (8-15), and 12 other compounds (16-27). Compound 1 is a new isobenzofuranone. The structures of the compounds were determined by analyzing their HRESIMS, 1D- and 2D-nuclear magnetic resonance (NMR), electronic circular dichroism (ECD) and comparing them to the reported data.

Chemical Constituents of .

A new lignan () and a new phenolic glycoside (), together with eighteen known compounds (-), were isolated from . Their structures were unambiguously elucidated by spectroscopic techniques (UV, IR, 1D and 2D NMR), and HR-ESI-MS analysis, as well as by comparison with the literature. The insecticidal activity of these compounds was evaluated against Koch, and the results showed that compounds , , , and had a weak inhibitory effect at a concentration of 1 mg/mL after treatment for 24 h.

BMSC-derived exosomal CD73 mediated macrophage polarization promotes osteoblastic differentiation in diabetes.

Stem cell-derived exosomes have exhibited promise for applications in tissue regeneration. However, the osteogenic ability of exosomes derived from bone marrow mesenchymal stem cells(BMSCs) in diabetes is impaired. Exosomes play a role in intercellular communication and affect the functional status of many adjacent cells.

Associations between maternal glycemic level and lipid profile and adverse pregnancy outcomes in women with type 2 diabetes mellitus: a retrospective study from China.

Purpose: To investigate the associations between maternal glucose and lipid metabolism and adverse pregnancy outcomes in women with type 2 diabetes mellitus (T2DM).

Methods: A retrospective cohort of pregnant women with T2DM who delivered at Peking University First Hospital was included. Univariate and multivariate logistic regression were used to identify risk factors.

Interplay between membrane system and biomolecular condensates in plant.

Biomolecular condensates, which lack surrounding membranes but function to concentrate proteins and nucleic acids, play crucial roles in various biological processes. These condensates, once considered membrane-less, are now recognized for their extensive interactions with membranes, influencing membrane structure and function. This review summarizes recent findings from research articles, highlighting the significance of the interplay between membrane system and biomolecular condensates.

ARID1A loss enhances sensitivity to c-MET inhibition by dual targeting of GPX4 and iron homeostasis, inducing ferroptosis.

ARID1A, a subunit of the SWI/SNF chromatin-remodeling complex, functions as a tumor suppressor in various cancer types. Owing to its high frequency of inactivating mutations, ARID1A has emerged as a promising target for the development of anticancer drugs. In this study, we report that ARID1A-deficient colorectal cancer (CRC) cells induce synthetic lethality when treated with inhibitors of c-MET receptor tyrosine kinase.

Association between S100A12 and risk of peripheral arterial disease in patients with dyslipidemia: a cross-sectional study.

Objective: S100A12 acts as a pro-inflammatory agent in vivo, with a close relationship with plaque formation in patients with acute coronary syndrome (ACS), end-stage renal disease, and diabetes. Peripheral arterial disease (PAD) can lead to mobility difficulties and ultimately disability and amputation. The association between S100A12 and risk of peripheral arterial disease remains unclear.

The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis.

To survive under adverse conditions, plants form stress granules (SGs) to temporally store mRNA and halt translation as a primary response. Dysregulation in SG disassembly can have detrimental effects on plant survival after stress release, yet the underlying mechanism remains poorly understood. Using Arabidopsis as a model system, we demonstrate that the β subunit of adaptor protein (AP) -3 complex (AP-3β) interacts with the SG core RNA-binding proteins Tudor staphylococcal nuclease 1/2 (TSN1/2) both in vitro and in vivo.

Bisabolane Sesquiterpenes with Anti-Chlamydial Activity Isolated from .

are obligate intracellular bacterial pathogens affecting humans and animals, causing miscarriage, stillbirth, or weak fetuses in the late stages of pregnancy of goats and sheep. Because there is no commercial vaccine for chlamydia in animals, drug treatment has become the most effective curative method. Natural products, also known as secondary metabolites, are becoming one of the main sources used in new drug development because of their structural diversity and biodiversity.

Liraglutide promotes osteogenic differentiation of mesenchymal stem cells by inhibiting M1 macrophage polarization and CXCL9 release in vitro.

As a GLP-1 receptor agonist widely used in treating type 2 diabetes, liraglutide shows potential applications in bone tissue engineering. This study investigated liraglutide's direct effects on rat bone marrow mesenchymal stem cells (BMSCs) osteogenic differentiation and its regulatory mechanism through macrophage polarization. Results showed that liraglutide significantly enhanced BMSC migration and osteogenic differentiation.

Naturally Occurring Cleistanthane Diterpenoids and Their Biological Activities.

Cleistanthane diterpenoids, an important class of active natural products, are characterized by a 6/6/6 tricycle system and contain a variety of functional groups. Studies have shown that cleistanthane diterpenoids are widespread in various higher plants and endophytic fungi and exhibit a wide range of biological activities. This review provides extensive coverage of naturally occurring cleistanthane diterpenoids discovered from 1973 to 2024 and sheds light on the sources, structures, biological effects, and primary molecular mechanisms of them and provides a useful reference for the further study and development of these compounds.

Jatrophane diterpenoids from Jatropha curcas with multidrug resistance reversal activity.

Nine jatrophane diterpenoids, including six previously undescribed compounds, were extracted and purified from whole plants of Jatropha curcas L. Their structures including absolute configurations were characterized by spectroscopic, quantum chemical Nuclear Magnetic Resonance Spectroscopy, Electronic Circular Dichroism calculation, and Single Crystal X-Ray Diffraction methods. These compounds were evaluated for their ability to reverse multidrug resistance.

A blood glucose fluctuation-responsive delivery system promotes bone regeneration and the repair function of Smpd3-reprogrammed BMSC-derived exosomes.

Blood glucose fluctuation leads to poor bone defect repair in patients with type 2 diabetes (T2DM). Strategies to safely and efficiently improve the bone regeneration disorder caused by blood glucose fluctuation are still a challenge. Neutral sphingophospholipase 2 (Smpd3) is downregulated in jawbone-derived bone marrow mesenchymal stem cells (BMSCs) from T2DM patients.

ARID1A loss sensitizes colorectal cancer cells to floxuridine.

The loss-of-function mutation of AT-rich interactive domain 1A (ARID1A) frequently occurs in various types of cancer, making it a promising therapeutic target. In the present study, we performed a screening of an FDA-approved drug library in ARID1A isogenic colorectal cancer (CRC) cells and discovered that ARID1A loss sensitizes CRC cells to floxuridine (FUDR), an antineoplastic agent used for treating hepatic metastases from CRC, both in vivo and in vitro. As a pyrimidine analogue, FUDR induces DNA damage by inhibiting thymidylate synthase (TS) activity.

A Novel Plant-Derived Biopesticide Mitigates Root Rot of by Modulating the Rhizosphere Microbiome and Root Metabolome.

root rot caused by the species complex significantly affects the yield and quality of , a valuable medicinal herb. Traditional management primarily relies on chemical fungicides, which have led to pathogen resistance, environmental hazards, and concerns regarding public health and the active components in . This study explores the efficacy of a novel plant-derived biopesticide Shi Chuang Zhi Feng Ning (T1; SCZFN), alongside wettable powder (T2) and a chemical fungicide (T3), in controlling root rot and understanding their impacts on the rhizosphere microbial community and root metabolome.

Electron Microscopy Analysis of Membraneless Condensates in Plant Cells.

Biomolecular condensates are triggered by multivalent interactions conferred by the intrinsically disordered regions and/or interacting domains within the constituents. While light microscopy has provided powerful tools to study the dynamics of intracellular condensates, electron microscopy (EM) gives more detailed insights into their ultrastructure and spatial connectivity with membrane system. In this chapter, we describe the methods for detecting the membraneless condensates in plant cells by high-pressure freezing -based EM coupled with immuno-gold labeling and correlative light electron microscopy techniques, which may benefit researchers in future studies.

A condensates-to-VPS41-associated phagic vacuoles conversion pathway controls autophagy degradation in plants.

Autophagy is a universal degradation system in eukaryotic cells. In plants, although autophagosome biogenesis has been extensively studied, the mechanism of how autophagosomes are transported to the vacuole for degradation remains largely unexplored. In this study, we demonstrated that upon autophagy induction, Arabidopsis homotypic fusion and protein sorting (HOPS) subunit VPS41 converts first from condensates to puncta, then to ring-like structures, termed VPS41-associated phagic vacuoles (VAPVs), which enclose autophagy-related gene (ATG)8s for vacuolar degradation.

Comparative Study on the Satisfaction of Healthcare Service Providers with the Synergistic Development of Rural Healthcare Systems in China: Medical Alliance Counties vs. Non-Medical Alliance Counties.

Introduction: This study aimed to explore whether the establishment of county medical alliances can improve satisfaction with the vertical integration of healthcare systems among rural medical and healthcare service provider managers and service providers. Our study also sought to provide recommendations for the sustainable development of vertical integration in healthcare systems.

Methods: A semi-structured interview with 30 healthcare service providers was employed in this research, and Nvivo software was utilized to analyze factors that influence vertical integration.