Simultaneous Lymphoepithelioma-Like and Plasmacytoid Subtypes of Urothelial Carcinoma, Along With Prostatic Adenocarcinoma With Clinical Follow-Up.

Lymphoepithelioma-like urothelial carcinoma (LELUC) and plasmacytoid urothelial carcinoma (PUC) are rare subtypes. We report a case of simultaneous urothelial carcinoma composed of LELUC and PUC subtypes, along with prostatic adenocarcinoma, with successful clinical management by immunotherapy. The patient, a 54-year-old man with a 40 pack-year smoking history, presented with gross hematuria and dysuria.

Active Learning-enhanced Deep Neural Network (AL-DNN) for uncertainty analysis of landfill leakage risk.

Landfills serve both as essential infrastructure for solid waste disposal and as potential sources of significant environmental risk. Leachate leakage has become a global concern due to its adverse impact on groundwater quality and associated public health threats. Accurate assessment of concealed leakage and its uncertainty is critical for effective risk management.

A multifunctional biomimetic nanoplatform combined with immune checkpoint blockade for triple-negative breast cancer immunotherapy through inhibiting polarization of M2 macrophages.

Immune checkpoint inhibitor (ICI) therapy has become a hopeful treatment for triple-negative breast cancer (TNBC). However, most patients exhibit a low immune response. Tumor-associated fibroblasts (TAFs) suppress anti-tumor immune responses by encouraging the polarization of M2 macrophages, which diminishes the therapeutic efficacy of ICIs.

Recent Advances in Host Immune Mechanisms Against Infection.

() is a foodborne Gram-positive pathogenic bacterium that primarily causes clinical manifestations such as sepsis, meningitis, and monocytosis. The main susceptible populations include newborns, pregnant women, adults over 40 years old, and immunocompromised individuals. Currently, antibiotic therapy remains the primary clinical treatment, but in recent years the issue of antibiotic resistance has become increasingly prominent.

[Mechanism of lipopolysaccharide inhibiting scrotal wound healing under hypoxic conditions].

Objective: To investigate the effect of lipopolysaccharide (LPS) on the crawling ability of fibroblasts derived from the scrotal skin under hypoxic conditions, its impact on vascular regeneration during wound healing, and the potential mechanism underlying chronic non-healing scrotal wound caused by gram-negative bacterial infection.

Methods: We extracted peripheral blood buffy coats from healthy donors after venous blood centrifugation, divided them into four groups (normoxia, normoxia+LPS, hypoxia, and hypoxia+LPS) for pretreatment, and isolated the corresponding cultured supernatant. Then we measured the levels of VEGF-A, FGF and IL-8 secreted using enzyme-linked immunosorbent assay (ELISA), determined the cytotoxicity of different pretreated supernatants by lactate dehydrogenase (LDH) release assay, and detected the cell migration ability and vascular formation in human-umbical vein endothelial cells (HUVEC).

The distributions of aflatoxin B1 and zearalenone in the processed products of maize germs during cold pressed process and solvent extracted process.

Aflatoxin B1 (AFB1) and zearalenone (ZEN) in maize germs threaten the food and feed security. However, their distributions in the processed products of maize germs during cold pressed process and solvent extracted process have not been reported in detail. Nine germ samples were selected and dealt by two oil production technologies.

Comprehensive understanding long-term heavy metals release characteristics and environmental impacts of ultra-alkaline fly ash based roadbed materials.

China's aggressive air pollution control policies have led to the widespread generation of ultra-alkaline fly ash (UAFAs). Compared with conventional fly ash, the risk characteristics, release mechanisms, and control strategies of UAFAs during its reuse in construction materials-such as roadbeds-remain fundamentally different yet poorly understood. This study systematically evaluates the lifecycle groundwater contamination risks of UAFAs-recycled road materials through accelerated carbonation experiments coupled with reactive transport modeling and Monte Carlo uncertainty analysis.

[Establishment of tissue culture and rapid propagation system of Artemisia stolonifera].

As a high-quality moxibustion material, Artemisia stolonifera has high economic value and research prospects. However, due to difficulties in seed germination, its wild germplasm resources are sparsely distributed in China. This study used young stem segments grown in the current year to investigate the effects of explant sterilization, different combinations and concentrations of plant growth regulators on the proliferation and rooting of adventitious shoots, with the aim of constructing an in vitro rapid propagation technology system for A.

Navigating Social Influences: The Impact of Anticipated HPV Vaccination Stigma from Friends and Family on College Students' HPV Vaccination Intent.

Background: Family and friends play a dual role in supporting and hindering young adults' decisions about the human papillomavirus (HPV) vaccine. However, the mechanisms by which anticipated HPV vaccination stigma from these social circles affects vaccination intent remain largely understudied. This study applied the Information-Motivation-Behavioral Skills model to examine how anticipated HPV vaccination stigma from family and friends influences vaccination intent and its underlying mechanisms.

Circ-myh8/KAT7 Affects PANoptosis in Pulmonary Arterial Smooth Muscle Cells: Involvement of Super-Enhancers in FOSL2 Expression.

Background: Pulmonary hypertension is a pathophysiological condition characterized by multiple forms of regulated cell death. PANoptosis, which is an inflammation-driven mode of regulated cell death, is regulated by the PANoptosome. Super-enhancers (SEs) have been implicated in the pathology of pulmonary hypertension by modulating the transcriptional regulation of target genes.

Developing machine learning models for predicting cardiovascular disease survival based on heavy metal serum and urine levels.

Background: Environmental exposure to heavy metals, such as arsenic, cadmium, and lead, is a known risk factor for cardiovascular diseases.

Objective: We aim to examine the associations between heavy metal exposure and the mortality of patients with cardiovascular diseases.

Methods: We analyzed data from the NHANES 2003-2018, including urine and blood metal concentrations from 4,924 participants.

Hippocampal Transcriptome Analysis in a Mouse Model of Chronic Unpredictable Stress Insomnia.

: This study aimed to develop a model for understanding stress-induced sleep disturbances and to explore the potential interactions between sleep disturbances and mood disturbances. : The chronic unpredictable mild stress (CUMS) group was established using the CUMS method, while the CUMS+Noise group was subjected to an additional 8-h exposure to noise in conjunction with the CUMS protocol. Each group was tested for anxiety and depressive-like behavior using the open-field, elevated plus maze, tail suspension, and forced swimming tests in male C57BL/6J mice.

Halogen Atom (Cl, Br, and I) Doping Effects on the Electronic Character and OER Performance of BiVO as a Photoanode Nanomaterial for Solar Water Splitting: A Theoretical Study.

Halogen elements, as common nonmetal dopants, have attracted significant attention for their potential in creating highly active photocatalysts. To improve the electronic property of BiVO, a highly promising photoanode nanomaterial for solar water splitting, the halogen atom (Cl, Br, and I) doping strategy and underlying modification mechanisms were explored in detail by the first-principles calculations here. Formation energies confirmed that halogen doping at the O sites of BiVO is energetically favorable and can be realized experimentally.

Analyses of Human papillomavirus, Ureaplasma urealyticum, Chlamydia trachomatis, Neisseria gonorrhoeae, herpes simplex virus 2 and coinfections among male outpatients in Kunming, China.

Sexually transmitted infections (STIs) such as human papillomavirus (HPV), Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and herpes simplex virus type 2 (HSV-2) significantly affect male reproductive health. Coinfections among these pathogens may aggravate disease severity, yet data on their prevalence among male outpatients remain scarce. In this study, we investigated the prevalence of UU, HSV2, NG, CT, and HPV infections and coinfections in the external genitalia of male outpatients in Kunming, Yunnan.

Shengxian decoction alleviates cyclophosphamide-induced immunosuppression via improving B cell-mediated immune responses.

Background: Chemotherapy is a prevalent and extensively utilized cancer treatment modality. However, it can result in immunosuppression. Shengxian Decoction (SXD) is a Traditional Chinese Medicine formula that has been demonstrated to improve immunosuppression caused by chemotherapy in clinical settings.

Enhanced Electrocatalytic Carbon Dioxide Reduction Activity via Local Charge Environment Regulation of Active Sites with Rational Functionalization.

Covalent organic frameworks (COFs) are a new emerging class of electrochemical catalysts for the CO reduction reaction (CORR) with fascinating structural tunability. In this work, to dig more detailed information about the effect of local charge environment regulation of active sites via structure modification on the catalytic performance of COFs for CORR, the Gibbs free-energy change (Δ) of each elementary reaction step involved in the CORR and competitive hydrogen evolution reaction (HER) on COF366-Co and its derivatives were examined theoretically. It is observed that the valence band maximum (VBM) energy level of the COFs is increased by incorporation of electron-donating groups, and then the charge distribution on the Co center of COF366-Co is increased due to the increased charge-transfer amount from the electron-occupied N sp hybrid orbitals to the empty Co orbitals.

Development and validation of a label-free HPLC-CAD method to determine total sialic acid in therapeutic proteins.

Sialylation plays a crucial role in influencing the pharmacokinetic, chemical, physical, and immunogenic properties of therapeutic proteins and is commonly tracked through total sialic acid analysis. In this study, we developed a novel label-free HPLC method to monitor total sialic acid levels in protein drug products. The method utilizes a replaceable, pre-column C18 cartridge that removes protein in-line, preventing protein interference with sialic acid and contamination of the analytical column and enabling direct sample injection.

Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice.

Background: Processes related to how the intracranial microvasculature initiates brain‒peripheral crosstalk for subsequent blood‒brain barrier (BBB) dysfunction at an early stage after subarachnoid hemorrhage (SAH) ictus are still unknown. This study elucidated the effect and potential mechanism of intracranial microvasculature-mediated T-cell infiltration on BBB function after SAH.

Methods: Publicly available single-cell RNA sequencing data related to SAH ( https://ngdc.

Transcriptome Analysis of the Gene Family in and Regulation of Militarine Synthesis Under Sodium Acetate and Salicylic Acid Treatments.

Calmodulin-like proteins (CMLs) are essential for calcium signal transduction in plants, influencing growth, development, stress responses, and the regulation of medicinal secondary metabolites. Despite their importance, the roles of genes in have not been characterized. This study aimed to elucidate the composition and function of the gene family in , identifying 38 genes across eight subfamilies.

Functions of the fusogenic and non-fusogenic activities of Syncytin-1 in human physiological and pathological processes.

Human endogenous retroviruses (HERVs), which represent the genetic remnants of ancient viral infections, constitute approximately 8 % of the human genome. Among the proteins encoded by these viruses, Syncytin-1, encoded by the env gene of the HERV-W family, functions as a vital fusion protein in placental development, in which it plays a pivotal role in facilitating the fusion of trophoblast cells to form the syncytiotrophoblast that is essential for maintaining the structural integrity and functional viability of the placenta. Recent studies have shown that in addition to its expression in the placenta, Syncytin-1 also plays key roles in a range of different tissues and cell types, influencing biological processes such as cell proliferation, apoptosis, and immune regulation.

Association of hemoglobin-to-red blood cell distribution width ratio with mortality in critically Ill patients with heart failure and acute kidney injury: insights from the MIMIC-IV database.

Background: The association between the hemoglobin-to-red cell distribution width ratio (HRR) and mortality in critically ill patients with heart failure (HF) and acute kidney injury (AKI) remains uncertain. This research focuses on exploring the association between HRR and both short-term and long-term all-cause mortality in these patients.

Methods: Participants were selected from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database and categorized into tertiles based on HRR values.

/-Type Doping Modulation of the Adsorption and Selection Behavior of Harmful Gas Molecules on the Surface of SWCNTs for Enhanced Gas-Sensing Performance.

Single-walled carbon nanotubes (SWCNTs) are a promising candidate material for detecting harmful gases due to their unique advanced character, but their gas-sensing properties still need to be improved. With the aim of exploring more effective modulation ways to improve the gas-sensing behavior of SWCNTs, the surface doping effects of the sodium (Na) atom, a typical -type dopant, and tetracyanoethylene (TCNE), a typical -type dopant, on the electronic and sensing properties of (7,3), (6,5), and (7,5) SWCNTs for NO, SO, NO, CO, HS, and NH were examined theoretically with density functional theory (DFT) calculations. It is found that the decoration of SWCNTs with Na/TCNE dopant is energetically favorable, with enhanced/lowered frontier energy levels.

Effect of four-stage refinement with fortified measures to inhibit oil oxidation and purification on the quality of anchovy oil.

Traditional refining processes of crude anchovy oil uses high temperatures. In order to inhibit oil oxidation, intensified measures should be taken. The impact of traditional four-stage refinement on anchovy oil quality have not yet been systematically revealed.

SRSF4-Associated ca-circFOXP1 Regulates Hypoxia-Induced PASMC Proliferation by the Formation of R Loop With Host Gene.

Background: Pulmonary hypertension (PH) is a rare and fatal disease, the pathological changes of which include pulmonary arterial smooth muscle cell (PASMC) proliferation, which is the pathological basis of pulmonary vascular remodeling. Studies have demonstrated that chromatin-associated circRNA can regulate a variety of biological processes. However, the role of chromatin-associated circRNA in the proliferation of PH remains largely unexplored.