A Novel Trivalent BVDV mRNA Vaccine Displayed by Virus-like Particles Eliciting Potent and Broad-Spectrum Antibody Responses.

: Bovine viral diarrhea virus (BVDV) causes significant economic losses in the cattle industry worldwide. The current vaccines have limited efficacy against diverse BVDV genotypes. Currently, multi-antigen target design and nanocarrier display technologies can provide ideas for broad-spectrum and efficient BVDV vaccine design.

Preparation of Monoclonal Antibodies Against the gD Protein of Feline Herpesvirus Type-1 by mRNA Immunization.

This study aimed to develop monoclonal antibodies (mAbs) against the gD protein of FHV-1 for rapid and specific virus detection. The gD protein, a highly conserved part of the FHV-1 envelope, is crucial for viral entry into host cells, making it an ideal detection target. We immunized BALB/c mice with an mRNA vaccine encoding the gD gene, achieving a serum antibody titer of 1:140,000 after three immunizations.

Ubiquitous memory augmentation via mobile multimodal embedding system.

Forgetting is inevitable in human memory. Recently, multimodal embedding models have been proposed to vectorize multimodal reality into a unified embedding space. Once generated, these embeddings allow mobile users to quickly retrieve relevant information, effectively augmenting their memory.

Added value of multiparametric MRI for diagnosing subcentimeter functional adrenal nodules in primary aldosteronism using CXCR4-targeted PET/MRI.

Objective: This study aims to evaluate the clinical utility of Ga-pentixafor PET/MRI in diagnosing primary aldosteronism (PA) in functional adrenal nodules, with a particular focus on subcentimeter nodules. Additionally, the diagnostic performance of integrated PET/MRI is compared to single-modality PET imaging.

Methods: A total of 62 patients with clinical suspicion of PA were enrolled from a tertiary hospital in China.

An mRNA vaccine provides effective protection against Duck Tembusu Virus infection.

As an emerging flavivirus, Duck Tembusu virus (DTMUV) causes severe neurological disorder and acute egg drop syndrome in poultry. Herein, we report the development of a highly effective mRNA vaccine against DTMUV using lipid nanoparticle (LNP) delivery technology. We engineered an mRNA construct encoding the pre-membrane (prM) and envelope (E) proteins of DTMUV, with an upstream signal peptide to enhance secretion.

The mRNA vaccine expressing fused structural protein of PRRSV protects piglets against PRRSV challenge.

The swine industry experiences substantial economic losses annually due to the porcine reproductive and respiratory syndrome virus (PRRSV). The limited protective efficacy of existing commercial vaccines against epidemic PRRSV underscores the urgent need for innovative solutions. The mRNA vaccines, which elicit robust immune responses, have emerged as a promising avenue in vaccine development.

Design and Development of a Novel Oral 4'-Fluorouridine Double Prodrug VV261 against SFTSV.

4'-Fluorouridine (4'-FU), despite demonstrating potent anti-SFTSV efficacy in vitro and in vivo, faces hindrances in its further development as a promising drug due to its weak chemical stability. Here, we report the discovery and development of VV261, a novel 4'-FU double prodrug with three isobutyryl groups on the ribose moiety and a nicotinoyloxymethyl group linked to the imide-nitrogen on the base moiety, exhibiting notable chemical stability and favorable pharmacokinetic properties. In SFTSV-infected mice, VV261 at 5 mg/kg/d for 7 days demonstrated complete protection against lethal SFTSV infection, prevented weight loss, and even a 2 day treatment significantly reduced both viral RNA copies and infectious virus titers in multiple organs, and notably alleviated splenic tissue lesions.

Transcriptome Analysis of the Gene Family in and Regulation of Militarine Synthesis Under Sodium Acetate and Salicylic Acid Treatments.

Calmodulin-like proteins (CMLs) are essential for calcium signal transduction in plants, influencing growth, development, stress responses, and the regulation of medicinal secondary metabolites. Despite their importance, the roles of genes in have not been characterized. This study aimed to elucidate the composition and function of the gene family in , identifying 38 genes across eight subfamilies.

Integrated transcriptomics and metabolomics provide insights into the biosynthesis of militarine in the cell suspension culture system of Bletilla striata.

Militarine is a monomer molecule with abundant and distinctive biological properties, also the lead member of secondary metabolites in Bletilla striata, while its biosynthesis mechanism is still unknown. To improve the production efficiency of militarine, sodium acetate and salicylic acid (SA) were introduced as elicitors into the suspension-cultured callus of B. striata.

Dynamics of wheat rhizosphere microbiome and its impact on grain production across growth stages.

Crop plant microbiomes are increasingly seen as important in plant nutrition and health, and a key to maintaining food productivity. Currently, little is known of the temporal changes that occur in the wheat rhizosphere microbiome as the plant develops, and how this varies among different sites. We used a pot-based mesocosm experiment with the same modern wheat cultivar grown in eight soils from across the North China Plain, a major wheat producing area.

Unveiling the Role of β-Glucosidase Genes in 's Secondary Metabolism: A Genome-Wide Analysis.

β-glucosidases (BGLUs) are abundant enzymes in plants that play pivotal roles in cell wall modification, hormone signal transduction, secondary metabolism, defense against herbivores, and volatile compound release. , a perennial herb revered for its therapeutic properties, lacks a comprehensive analysis of its gene family despite the critical role these genes play in plant secondary metabolism. This study aims to perform a genome-wide analysis of the gene family in () to elucidate their functions and regulatory mechanisms in secondary metabolite biosynthesis.

Facile Synthesis of Soft Core-Shell Nanospheres for Constructing Multiresponsive Photonic Crystal Security Patterns.

Responsive photonic crystals (RPCs) presenting tunable structural colors under external stimuli are widely applied in the fields of dynamic displays, sensors, and anticounterfeiting. However, the development of multiresponsive photonic crystal (MRPC) films possessing versatile variable optical properties remains a significant challenge due to the tedious synthetic procedure of multifunctional building blocks and complex assembly processes, thereby constraining their extensive applications. In the present work, a series of soft nanospheres with a hydrophobic cores and responsive hydrophilic shells have been synthesized by a facile one-step surfactant-free emulsion polymerization method.

PROTAC technology: From drug development to probe technology for target deconvolution.

Drug development remains a critical focus within the global pharmaceutical industry. To date, more than 80 % of disease targets are considered difficult to target. The emergence of PROTAC technology has, to some extent, alleviated this challenge.

Digital non-Foster-inspired electronics for broadband impedance matching.

Narrow bandwidths are a general bottleneck for applications relying on passive, linear, subwavelength resonators. In the past decades, several efforts have been devoted to overcoming this challenge, broadening the bandwidth of small resonators by the means of analog non-Foster matching networks for radiators, antennas and metamaterials. However, most non-Foster approaches present challenges in terms of tunability, stability and power limitations.

CCT6A alleviates pulmonary fibrosis by inhibiting HIF-1α-mediated lactate production.

Idiopathic pulmonary fibrosis (IPF) is a lethal progressive fibrotic lung disease. The development of IPF involves different molecular and cellular processes, and recent studies indicate that lactate plays a significant role in promoting the progression of the disease. Nevertheless, the mechanism by which lactate metabolism is regulated and the downstream effects remain unclear.

The rare cause of ST segment elevation in left precordial leads - Diagnostic clues from subtle waveforms.

We report a case of ST segment elevation in left precordial leads with a convex shape caused by a rare etiology. By carefully analyzing the electrocardiogram (leads I, II, V3 to V9) of a patient with convex ST segment elevation in the left-sided chest leads, relevant etiological clues were derived. The findings were further supported by cardiac ultrasound and cardiac magnetic resonance imaging, ruling out other common causes.

The role of sirtuin1 in liver injury: molecular mechanisms and novel therapeutic target.

Liver disease is a common and serious threat to human health. The progression of liver diseases is influenced by many physiologic processes, including oxidative stress, inflammation, bile acid metabolism, and autophagy. Various factors lead to the dysfunction of these processes and basing on the different pathogeny, pathology, clinical manifestation, and pathogenesis, liver diseases are grouped into different categories.

Molecular mechanism of miRNA mediated biosynthesis of secondary metabolites in medicinal plants.

Plant secondary metabolites are important raw materials for the pharmaceutical industry, and their biosynthetic processes are subject to diverse and precise regulation by miRNA. The identification of miRNA molecules in medicinal plants and exploration of their mechanisms not only contribute to a deeper understanding of the molecular genetic mechanisms of plant growth, development and resistance to stress, but also provide a theoretical basis for elucidating the pharmacological effects of authentic medicinal materials and constructing bioreactors for the synthesis of medicinal secondary metabolite components. This paper summarizes the research reports on the discovery of miRNA in medicinal plants and their regulatory mechanisms on the synthesis of secondary metabolites by searching the relevant literature in public databases.

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants.

Tuber plants are of great significance in the world as human food crops. Polysaccharides, important metabolites in tuber plants, also serve as a source of innovative drugs with significant pharmacological effects. These drugs are particularly known for their immunomodulation and antitumor properties.

Successful treatment with bortezomib in combination with dexamethasone in a middle-aged male with idiopathic multicentric Castleman's disease: A case report.

Multicentric Castleman disease (MCD) is a heterogeneous, life-threatening disease. A subgroup of HIV-negative and HHV-8-negative MCD is defined as idiopathic MCD (iMCD) with a poor prognosis. Here we report an unusual case of a 47-year-old male patient with iMCD who experienced multiple treatment regimens such as chemotherapy, immunomodulatory therapy, and targeted therapy, all of which were considered ineffective.