An infection microenvironment-responsive multifunctional nanocomposite for chronic wound treatment through redox homeostasis and macrophage regulation.

Due to repeated microbial infection and an immunosuppressive microenvironment, chronic wound healing can be significantly hindered. The development of multifunctional nanomaterials capable of simultaneous antibacterial and immunomodulatory effects continues to present challenges. Herein, a multifunctional nanocomposite, MXene/CuO, was developed that integrates photothermal therapy (PTT), chemodynamic therapy (CDT), and sonodynamic therapy (SDT), providing tri-modal therapy for synergistic antibacterial and immunomodulatory wound healing.

Profiling RNA subcellular localization in situ by TATA-seq.

Membrane-less organelles, dynamic subcellular structures formed by RNA and RNA-binding proteins (RBPs) undergoing liquid-liquid phase separation (LLPS), play key roles in biological processes such as RNA degradation in processing bodies (P-bodies), translation inhibition in stress granules, and RNA splicing in nuclear speckles. However, the study of RNA species within these organelles has been hindered by the absence of simple, sensitive, and specific methodologies. Here, we introduce Target Transcript Amplification and Sequencing (TATA-seq), a novel strategy for precisely profiling RNA in membrane-less organelles via in situ targeted transcription and linear amplification.

Biomimetic Nanoparticles with Dual Targeting for Hepatocellular Carcinoma Promote Tumor Immune System Activation by Enhancing Extracellular ATP Homeostasis.

Chemodynamic therapy (CDT) plays a crucial role in transforming the tumor microenvironment by inducing immunogenic cell death (ICD) to eliminate cancer cells. Nonetheless, the effectiveness of CDT in eliciting antitumor immunity is somewhat constrained. The persistent presence of immunosuppressive cells, such as myeloid-derived suppressor cells (MDSCs), in the tumor offsets some of the ICD effects triggered by CDT.

Quantifying ultrasound echogenicity difference for accurate chronic kidney disease diagnosis.

Background: This study aims to evaluate the diagnostic ability of quantitative renal echogenicity for chronic kidney disease (CKD) using ultrasounds.

Methods: Between September 2018 and December 2019, patients with kidney disease at our hospital underwent ultrasound imaging and serum creatinine tests. Five radiologists assessed patient ultrasound image echogenic characteristics for CKD diagnosis.

Recognizing the value of municipal solid waste resource utilization in delivering carbon dioxide removal.

This paper attempts to verify that the resource utilization of municipal solid waste (MSW) is a feasible method to reduce greenhouse gas emissions. We combine the data and empirical study results of Shanghai, China, to calculate and project Shanghai's contribution to China's 2030 carbon peak commitment through MSW resource utilization process from 2021 to 2030. The calculation uses IPCC recommended methods.

Nanomedicine-Driven Modulation of Reductive Stress for Cancer Therapy.

Redox balance is crucial for cellular function and adaptation to environmental changes, with its disruption playing a key role in the progression of various diseases, including cancer. While oxidative stress caused by excessive reactive oxygen species (ROS) has been widely studied and targeted in cancer therapies, such approaches face significant challenges within the tumor microenvironment. On the opposite end, reductive stress results from an overabundance of reducing equivalents, disrupting normal ROS-dependent signaling pathways and leading to cellular dysfunction.

Identification and Expression Analysis of G-Protein-Coupled Receptors Provide Insights into Functional and Mechanistic Responses to Herbivore-Induced Plant Volatiles of .

Herbivore-induced plant volatiles (HIPVs) play a pivotal role in mediating tritrophic interactions between plants, herbivores, and their natural enemies. , a parasitic mite targeting the egg stage of , has emerged as a promising biocontrol agent. However, its ability to detect -derived HIPVs for host insect localization remains unclear.

Unraveling the mechanism of greenhouse gas emissions during allelochemical-triggered inhibition of cyanobacteria recovery.

Climate change amplifies cyanobacterial blooms, creating a feedback loop between warming and bloom intensity. This dynamic emphasizes the need to address greenhouse gas (GHG) emissions during in-situ cyanobacterial control, minimizing algicides use and its long-term climatic impacts. However, research on the dose-response of algicides on GHG emissions is limited.

Indole competitively inhibits quorum sensing and triggers oxidative stress to suppress Microcystis blooms.

The proliferation of cyanobacterial blooms poses a significant threat to aquatic ecosystems and drinking water safety. Traditional control strategies mainly focus on environmental factors and overlook microbial communication networks. This study systematically elucidated the quorum sensing (QS) system in Microcystis aeruginosa and identified a previously unknown QS signaling molecule N-carboxy-C-homoserine lactone (N-carboxy-C-HSL), and its homologous synthase and receptor in the genome for MaeI/MaeR through ultra-high resolution mass spectrometry and genomics methods.

Plasma proteins and different onset subtype of COPD: Proteome-wide Mendelian randomization study and co-localization analyses.

Several studies have reported a strong association between plasma proteins and chronic obstructive pulmonary disease (COPD). However, the directionality and causality of the association and whether proteins effected COPD remain unclear. Therefore, we used Proteome-wide Mendelian randomization (MR) study and co-localization analyses to estimate the casual relationship between them.

DYRK1B phosphorylates FOXO1 to promote hepatic gluconeogenesis.

Dual-specificity tyrosine phosphorylation-regulated kinase 1B (DYRK1B), a member of the CMGC group of kinases, is linked to metabolic syndrome, though the underlying molecular mechanisms remain unclear. In this study, we show that Dyrk1b expression is induced in the liver by fasting and in diabetic mice. Through both in vivo and in vitro experiments, we demonstrate that DYRK1B promotes hepatic gluconeogenesis and glucose intolerance.

Lactate dehydrogenase to albumin ratio and prognosis in patients with acute exacerbation of chronic obstructive pulmonary disease: a retrospective cohort study.

Background: Chronic obstructive pulmonary disease (COPD) is a global public health challenge and a major cause of death. The lactate dehydrogenase to albumin ratio (LAR) is a simple and practical indicator of disease prognosis, but its prognostic value in acute exacerbation of COPD (AECOPD) remains unclear. Therefore, we aimed to explore the prognostic value of LAR for the short-term all-cause mortality risk in patients with AECOPD.

A stacking ensemble machine learning model for predicting postoperative axial pain intensity in patients with degenerative cervical myelopathy.

Machine learning (ML) has been extensively utilized to predict complications associated with various diseases. This study aimed to develop ML-based classifiers employing a stacking ensemble strategy to forecast the intensity of postoperative axial pain (PAP) in patients diagnosed with degenerative cervical myelopathy (DCM). A total of 711 consecutive postoperative DCM patients were included between 2016 and 2024, and after excluding patients who did not meet the inclusion criteria and those who met the exclusion criteria, a total of 484 patients were ultimately included in this study.

Relationship between diabetes-related clinical characteristics and preserved ratio impaired spirometry (PRISm): findings from NHANES 2007-2012.

Introduction: To analyse the relationship between diabetes, its severity (including blood glucose levels, disease duration, antidiabetic drug use and number of comorbidities) and preserved ratio impaired spirometry (PRISm) using data from the National Health and Nutrition Examination Survey (NHANES).

Methods: This cross-sectional study collected data from the NHANES database from 2007 to 2012. PRISm was defined as having a forced expiratory volume in 1 s (FEV1) to forced vital capacity (FVC) ratio ≥0.

[Genome-wide identification, characterization, and expression analysis of MAPK genes in response to infection in ].

In recent years, the spread of clubroot disease caused by infection has seriously affected the yield and quality of (L.) Czern..

[Pharmacokinetics and anti-inflammatory activity of cannabidiol/ γ-polyglutamic acid-g-cholesterol nanomicelles].

In this study, the pharmacokinetic characteristics and tissue distribution of cannabidiol(CBD)/γ-polyglutamic acid-g-cholesterol(γ-PGA-g-CHOL) nanomicelles [CBD/(γ-PGA-g-CHOL)NMs] were investigated by pharmacokinetic experiments, and the effect of CBD/(γ-PGA-g-CHOL)NMs on the lipopolysaccharide(LPS)-induced inflammatory damage of cells was evaluated by cell experiments. CBD/(γ-PGA-g-CHOL)NMs were prepared by dialysis. The CBD concentrations in the plasma samples of male SD rats treated with CBD and CBD/(γ-PGA-g-CHOL)NMs were investigated, and the pharmacokinetic parameters were calculated and compared.

Superhydrophobic sponge-like chitosan/CNTs/silica composite for selective oil absorption and efficient separation of water-in-oil emulsion.

Developing state-of-the-art materials with durability, superhydrophobicity, and superoleophilicity is essential for effective cleanup of oil spills and the treatment of oily wastewater. In this study, a novel superhydrophobic/superoleophilic chitosan-based composite was created by incorporating carbon nanotubes (CNTs) into a chitosan (CS) matrix and depositing superhydrophobic SiO nanoparticles, forming a sponge-like absorbent (SH-SiO@3CNTs/CS) for selective oil absorption and efficient oil/water separation. The optimal CNTs incorporation was comprehensively investigated to enhance the structural stability and mechanical strength of the chitosan/CNTs/silica composite.

Mesenchymal Stem Cells With Polydopamine-Coated NaGdF Nanoparticles with Ca Chelation Ability for Ischemic Stroke Therapy.

Mesenchymal stem cells (MSCs) transplantation is a promising therapeutic strategy for ischemic stroke. However, the survival of transplanted MSCs is often compromised by the excessive levels of reactive oxygen species (ROS) and calcium ions (Ca) in the ischemic microenvironment following blood flow occlusion. In this study, a protective strategy is developed using functional nanomaterials to escort and shield MSCs.

Effect of two novel GABAA receptor positive allosteric modulators on neuropathic and inflammatory pain in mice.

Loss of GABAergic inhibition in the spinal dorsal horn (SDH) is implicated in central sensitization and chronic pain. Both agonists and positive allosteric modulators (PAMs) of GABAA receptor are found to be effective in the management of chronic pain. In addition to benzodiazepines, neuroactive steroids (NASs) also act as PAMs through binding to unique sites of GABAA receptors.

Neuron Modulation by Synergetic Management of Redox Status and Oxidative Stress.

The transient receptor potential (TRP) channel is a key sensor for diverse cellular stimuli, regulating the excitability of primary nociceptive neurons. Sensitization of the TRP channel can heighten pain sensitivity to innocuous or mildly noxious stimuli. Here, reversible modulation of TRP channels is achieved by controlling both the light-induced photoelectrochemical reaction to induce neuronal depolarization, and antioxidants for neuronal protection.

Comparison of STAR and GOLD in Assessing Disease Severity Among High-Risk and COPD Patients: Evidence from Enjoying Breathing Program in China.

Background: The STAR staging standard has been demonstrated to have good performance in distinguishing mortality among patients at different stages. However, the effectiveness of STAR and GOLD staging in distinguishing disease severity in high-risk and COPD patients remained unclear.

Methods: Based on Enjoying Breathing Program data through June 2023, a total of 7.

The role of exosomes in the pathogenesis and management of diabetic kidney disease: a systematic review and meta-analysis.

Objective: This systematic review and meta-analysis aimed to synthesize the role of exosomes in the pathogenesis and management of diabetic kidney disease.

Methods: PubMed, Embase, Cochrane Library, and Web of Science were searched for studies that compared the levels of exosomes between patients with diabetic kidney disease and controls published up to 27 November 2023. Methodological quality was assessed using the JBI Appraisal Checklist for Case-Control Studies.