Genomic analysis of Pseudoalteromonas sp. SD03 reveals its potential in chitin hydrolysis.

Pseudoalteromonas sp. SD03, a marine bacterium capable of chitin degradation, was isolated from Antarctic surface water. Here, the genome of strain SD03 was sequenced and the chitin metabolic pathways were constructed.

Rheinheimera fenheensis sp. nov., Isolated from Fenhe River Basin, China.

Three Gram-stain-negative, aerobic, rod-shaped bacterial strains, FR7-31, FR7-34 and HH7-4, were isolated from sediment samples of the Fenhe River basin, China. The 16S rRNA gene sequence analysis revealed that strain FR7-31 shares 99.7% and 99.

Antagonistic effect of changing salinity and dissolved organic carbon on NO production via different pathways in saline lakes.

Saline lakes are experiencing significant changes in salinity and organic matter content due to climate change. However, the specific impacts of these environmental changes on the production processes of nitrous oxide (NO)-particularly nitrification and denitrification-in saline lake sediments are still poorly understood, leading to significant uncertainty in current estimates of greenhouse gas (GHG) emission from these ecosystems. Here, we employed N-isotope labeling, functional gene quantification, and structural equation modeling to elucidate NO production pathways and rates in surface sediments along a salinity gradient (0.

Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-Bioactivities.

Antarctic krill () is a nutrient-rich marine resource. Although several terrestrial proteases have been used to prepare Antarctic krill peptides (AKPs), there has been no report on the preparation of AKPs using a marine protease. Here, marine bacterial protease A69 was used to prepare AKPs with multi-bioactivities.

Contrasting sulfur isotope signatures in two arid basins separated by the Qilian mountains.

Global climate variations have intensified the transformation of salt lakes and saline-alkali playas in arid regions into major sources of saline-alkali dust storms, actively influencing atmospheric circulation and climate dynamics. This study focuses on two geographically close but environmentally distinct regions, the Qaidam Basin and the Alxa Plateau, situated on opposite sides of the Qilian Mountains. Surface salts were collected from both areas and analyzed for ion composition and sulfur isotope characteristics.

Severe old lumbar fracture and dislocation with cauda equina nerve transection: A case report.

Severe old lumbar fractures and dislocations with associated cauda equina nerve transection are rare clinical presentations which pose significant management challenges. This case report highlights a unique instance of such an injury, emphasizing novel surgical strategies for reconstruction and recovery. A 30-year-old male patient was admitted after sustaining a severe open fracture and degree IV posterior dislocation of L3 vertebra, accompanied by cauda equina nerve transection, spinal cord injury and multiple organ injuries.

Radium isotopes as tracers of lacustrine groundwater discharge: Review and prospects.

Lacustrine groundwater discharge (LGD) is a crucial component of lake hydrological budgets and serves as a significant source such as nutrients and pollutants. Naturally occurring radioactive radium isotopes (Ra, Ra, Ra, Ra) have emerged as valuable tracers for studying lacustrine groundwater discharge due to their distinctive geochemical properties. While radium isotopes were primarily utilized in studies of submarine groundwater discharge in the past, their "salt effect" characteristics have increasingly established them as essential tools for identifying and quantifying LGD.

Highly Efficient Yolk-Shell Photocatalyst Constructed by Integration of NiP and CuO Nanoparticles to Defective Metal-Organic Frameworks for Visible-Light-Driven Amine Oxidation.

Realizing the directional migration of photogenerated carriers plays an important role in improving the photocatalytic performance. Meanwhile, light-driven oxidative coupling of benzylamine under ambient conditions with an inexpensive catalyst is highly desirable for the industrial field. Herein, via in situ synthesis, defect engineering, and photodeposition, a yolk-shell nanostructured photocatalyst, NiP@OH-NH-UiO-66@CuO, featuring nickel phosphide (NiP) nanoparticles (NPs) trapped inside a defect engineered metal-organic framework (MOF, namely OH-NH-UiO-66) and CuO NPs adhering on the surface of MOFs, has been rationally fabricated for the achievement of spatial separation of oxidation/reduction cocatalyst in photocatalytic reaction systems.

sp. nov., a DNA-hydrolysing bacterium isolated from intertidal sediment.

A Gram-stain-negative, yellow-pigmented, non-flagellated, motile by gliding, aerobic and rod-shaped bacterial strain, designated SM2212, was isolated from intertidal sediment of Aoshan Bay in Qingdao, China. The strain grew at 5-35 °C and with 0.5-5.

Genomic analysis of Vreelandella sp. F11 reveals its role in alginate utilization.

Alginate, mainly produced by brown algae, is an important polysaccharide that supports the growth of marine bacteria. Vreelandella sp. F11 is a Gram-negative and aerobic marine bacterium, which was isolated from the brown algae sample collected from the Weihai coast, the Yellow Sea, China.

Direct Preparation of Alginate Oligosaccharides from Brown Algae by an Algae-Decomposing Alginate Lyase AlyP18 from the Marine Bacterium A3.

Alginate oligosaccharides (AOs), derived from alginate degradation, exhibit diverse biological activities and hold significant promise in various fields. The enzymatic preparation of AOs relies on alginate lyases, which offers distinct advantages. In contrast to the conventional use of sodium alginate derived from brown algae as the substrate for the enzymatic preparation of AOs, AO preparation directly from brown algae is more appealing due to its time and energy efficiency.

Latent Trajectories of Activities of Daily Living Disability and Associated Factors Among Adults with Post-Intensive Care Syndrome One Week After ICU Discharge.

Objective: To identify the latent trajectories of activities of daily living (ADL) disability and the influential factors among adults with post-intensive care syndrome (PICS).

Methods: We evaluated five-time longitudinal data about PICS diagnosed in 434 of 593 assessed patients (73.19%).

Genome-based reclassification of Chhetri . 2023 as a later heterotypic synonym of Gao . 2023.

DHUNG17 was compared with XHP0097 to examine the taxonomic relationship between the two type strains. The 16S rRNA gene sequence of DHUNG17 shared high similarity (99.9 %) to that of XHP0097.

The complete genome sequence of the planctomycetotal bacterium Bremerella sp. P1 with abundant genes involved in polysaccharide degradation.

Isolated from intertidal sediment of the Yellow Sea, China, Bremerella sp. P1 putatively represents a novel species within the genus Bremerella of the family Pirellulaceae in the phylum Planctomycetota. The complete genome of strain P1 comprises a single circular chromosome with a size of 6,955,728 bp and a GC content of 55.

Corrigendum: Intratumor tertiary lymphatic structure evaluation predicts the prognosis and immunotherapy response of patients with colorectal cancer.

[This corrects the article DOI: 10.3389/fimmu.2024.

Morphology Regulation of UiO-66-2I Supporting Systematic Investigations of Shape-Dependent Catalytic Activity for Degradation of an Organophosphate Nerve Agent Simulant.

Phosphonate-based nerve agents, as a kind of deadly chemical warfare agent, are a persistent and evolving threat to humanity. Zirconium-based metal-organic frameworks (Zr-MOFs) are a kind of highly porous crystalline material that includes Zr-OH-Zr sites and imitates the active sites of the phosphotriesterase enzyme, representing significant potential for the adsorption and catalytic hydrolysis of phosphonate-based nerve agents. In this work, we present a new Zr-MOF, UiO-66-2I, which attaches two iodine atoms in the micropore of the MOF and exhibits excellent catalytic activity on the degradation of a nerve agent simulant, dimethyl 4-nitrophenyl phosphate (DMNP), as the result of the formation of halogen bonds between the phosphate ester bonds and iodine groups.

Development and characterization of a novel specific monoclonal antibody against mink enteritis virus and its antigen epitope analysis.

This study prepared a novel monoclonal antibody (MAb) against mink enteritis parvovirus (MEV) and identified its antigen epitope. The antibody subclass is identified as IgG1, the titers of the MAb is up to 1:1 × 10 and keeps stably after low-temperature storage for 9 months or 11 passages of the MAb cells. The MAb can specifically recognize MEV in the cells in IFA, but not Aleutian disease virus (ADV) or canine distemper virus (CDV).

Phosphoglycerate mutase 1 promotes breast cancer progression through inducing immunosuppressive M2 macrophages.

Immunosuppressive tumor microenvironment (TME) contributes to tumor progression and causes major obstacles for cancer therapy. Phosphoglycerate mutase 1 (PGAM1) is a key enzyme involved in cancer metabolism while its role in remodeling TME remains unclear. In this study, we reported that PGAM1 suppression in breast cancer (BC) cells led to a decrease in M2 polarization, migration, and interleukin-10 (IL-10) production of macrophages.

Microbial difference and its influencing factors in ice-covered lakes on the three poles.

Most lakes in the world are permanently or seasonally covered with ice. However, little is known about the distribution of microbes and their influencing factors in ice-covered lakes worldwide. Here we analyzed the microbial community composition in the waters of 14 ice-covered lakes in the Hoh Xil region of northern Qing-Tibetan Plateau (QTP), and conducted a meta-analysis by integrating published microbial community data of ice-covered lakes in the tripolar regions (the Arctic, Antarctica and QTP).

Isotopic insights and integrated analysis for heavy metal levels, ecological risks, and source apportionment in river sediments of the Qinghai-Tibet Plateau.

The research was carried out to examine the pollution characteristics, ecological risk, and origins of seven heavy metals (Hg, As, Pb, Cu, Cd, Zn, and Ni) in 51 sediment samples gathered from 8 rivers located on the Qinghai-Tibet Plateau (QTP) in China. The contents of Hg and Cd were 5.0 and 1.

Effects of immunogenic cell death-inducing chemotherapeutics on the immune cell activation and tertiary lymphoid structure formation in melanoma.

Background: The infiltration and activation of immune cells in the tumor microenvironment (TIME) affect the prognosis of patients with cancer. Tertiary lymphoid structure (TLS) formation favors tumour- infiltrating-lymphocyte (TIL) recruitment and is regarded as an important indicator of good prognosis associated with immunotherapy in patients with tumors. Chemotherapy is currently one of the most commonly used clinical treatment methods.

The spatial landscape of T cells in the microenvironment of stage III lung adenocarcinoma.

This study aimed to provide more information for prognostic stratification for patients through an analysis of the T-cell spatial landscape. It involved analyzing stained tissue sections of 80 patients with stage III lung adenocarcinoma (LUAD) using multiplex immunofluorescence and exploring the spatial landscape of T cells and their relationship with prognosis in the center of the tumor (CT) and invasive margin (IM). In this study, multivariate regression suggested that the relative clustering of CT CD4 conventional T cell (Tconv) to inducible Treg (iTreg), natural regulatory T cell (nTreg) to Tconv, terminal CD8 T cell (tCD8) to helper T cell (Th), and IM Treg to tCD8 and the relative dispersion of CT nTreg to iTreg, IM nTreg to nTreg were independent risk factors for DFS.

sp. nov., isolated from deep-sea sediment of the Mariana Trench.

A Gram-stain-negative, aerobic, rod-shaped, non-flagellated, non-gliding bacterial strain, designated MT50, was isolated from a deep-sea sediment sample collected from the Mariana Trench. Optimal growth of strain MT50 was observed at 25 °C, pH 7.0-7.

The complete mitochondrial genome of the tropical sea urchin (Linnaeus, 1758) with two spine colors.

The complete mitochondrial genomes of two spine-color individuals, red and white, of the tropical sea urchin species (Linnaeus, 1758) were sequenced on Illumina system platform. The red-spined species had a genome size of 15,774 bp, while the white-spined species had a genome size of 15,723 bp. Both genomes contained 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes.