Genetic burden and multidimensional predictors in prenatal diagnosis of fetal congenital diaphragmatic hernia.

This study aims to assess the genetic burden of fetal congenital diaphragmatic hernia (CDH) and identify prenatal, perinatal, and postnatal predictors to improve early diagnosis, monitoring, and intervention. This study included 130 CDH fetuses who underwent invasive prenatal diagnosis, with fetal prognosis evaluated using imaging parameters such as observed-to-expected lung-to-head ratio (o/e LHR), observed-to-expected total lung volume (o/e TLV), and percent predicted lung volume (PPLV). Clinical outcomes included neonatal outcomes, extracorporeal membrane oxygenation (ECMO) requirement, and post-neonatal prognosis.

Restrictive strabismus caused by infantile myositis masquerading as Duane retraction syndrome.

We report a rare case of restrictive strabismus in a pediatric patient who was initially misdiagnosed with Duane retraction syndrome (DRS) because she had large-angle exotropia, restricted adduction, globe retraction, narrowing of the palpebral fissure, and downshoot on attempted adduction. Notably, the parents first observed strabismus at 12 months of age, with photographs taken before 6 months of age revealing no ocular misalignment. Despite the absence of typical inflammatory signs, the temporal discrepancy, characterized by delayed onset inconsistent with congenital DRS, prompted further investigation.

High-efficient refolding and purification of recombinant human interleukin-2 from inclusion bodies.

Human interleukin-2 (hIL-2) serves as a crucial cytokine in the treatment of cancer and autoimmune disorders. Nevertheless, the advancement of research and clinical applications involving this cytokine has been hindered by the constraints associated with the production of recombinant human interleukin-2 (rhIL-2). This study presents a scalable and robust purification protocol for rhIL-2 derived from inclusion bodies (IBs) in Escherichia coli.

mutations cause high myopia by triggering PI3K-AKT and NF-κB-mediated signaling pathway in humans and mice.

High myopia (HM) is a severe form of refractive error that results in irreversible visual impairment and even blindness. However, the genetic and pathological mechanisms underlying this condition are not yet fully understood. From a cohort of 1015 patients with HM in adolescents, likely pathogenic missense mutations were identified in the gene in four patients by whole exome sequencing.

Revealing the characteristic honey-like aroma formation in honey-scented black tea via molecular sensory analysis.

Honey-scented black tea is highly favored by consumers for its unique flavor; however, the key components and mechanisms underlying its characteristic aroma remain unclear. The aroma characteristics and key components of honey-scented black tea (MXBT), sweet-scented black tea (TXBT), and honey (FM) were elucidated, and an aroma flavor wheel was constructed. β-Damascenone, phenylacetaldehyde, and linalool oxide I were identified as the primary aroma compounds responsible for the honey-like scent of MXBT.

Intravenous Multifunctional Nanotherapy for Treating Dry Age-Related Macular Degeneration.

Retinal pigment epithelium (RPE) degeneration is the pathological hallmark of multifactorial dry age-related macular degeneration (dAMD). Mounting evidence implicates oxidative stress and aberrant activation of mammalian target of rapamycin (mTOR) as key drivers of this process. Recent studies have shown that simultaneous modulation of these pathways may offer therapeutic benefit.

Anti-stigma campaign: the COVID-19 stigma and associated factors among Chinese young students.

Introduction: COVID-19-related stigma, a persistent consequence of the pandemic, poses a significant threat to the well-being of individuals, particularly young students in crucial developmental stages. This study aimed to investigate the prevalence of stigma views towards COVID-19 patients among young students in Sichuan Province, China, following the lifting of pandemic restrictions, and explored the associated influencing factors.

Methods: A cross-sectional survey was conducted online involving 82,873 young students.

A molecular and spinal circuit basis for the functional segregation of itch and pain.

Recent advances reveal an extensive cellular diversity within the dorsal horn. How this complexity processes distinct sensations, like itch and pain, remains a fundamental question. We discovered hidden within a population of neurons expressing the gastrin-releasing peptide receptor (Grpr+), thought to be itch-specific, are highly homologous yet functionally distinct subtypes distinguished by expression of Tachykinin-1 (Tac1).

Plasma metabolites mediate the causal effect of carbohydrate intake on bone mineral density.

Osteoporosis is a widespread skeletal disorder characterized by reduced bone mineral density (BMD) and increased fracture risk. While dietary factors are known to influence bone health, the causal role of specific macronutrients-particularly carbohydrates-remains uncertain. This study aimed to investigate the causal effect of carbohydrate intake on BMD and to identify plasma metabolites that may mediate this association.

(±)-Talapyrones A-F: six pairs of dimeric polyketide enantiomers with unusual 6/6/6 and 6/6/6/5 ring systems from Talaromycesadpressus.

(±)-Talapyrones A-F (1-6), six pairs of dimeric polyketide enantiomers featuring unusual 6/6/6 and 6/6/6/5 ring systems, were isolated from the fungus Talaromyces adpressus. Their structures were determined by spectroscopic analysis and HR-ESI-MS data, and their absolute configurations were elucidated using a modified Mosher's method and electronic circular dichroism (ECD) calculations. (±)-Talapyrones A-F (1-6) possess a 6/6/6 tricyclic skeleton, presumably formed through a Michael addition reaction between one molecule of α-pyrone derivative and one molecule of C poly-β-keto chain.

Dispersion-engineered low-crosstalk metasurface for mid-infrared snapshot multi-spectral imaging.

Infrared spectroscopy covers the "fingerprint" region of molecular vibrations, offering non-destructive detection capabilities and excellent anti-interference performance, thereby providing robust technical support for material identification and target recognition. However, conventional infrared spectral imaging systems typically rely on complex wavelength separation or time-division filtering schemes, leading to challenges such as low energy utilization efficiency, inadequate system stability, and limited integration capabilities. Here, we present a dispersion-engineered metasurface with a quadratic phase profile, which maps spectral information directly onto spatial coordinates for single-shot spatial-spectral data acquisition.

Photochemical Transformation of Arsenic in the Presence of Low-Molecular-Weight Organic Acids and Antimony: Volatile Species Formation and Nanoparticle-Mediated Mechanism.

Arsenic (As), a well-known toxic element, often coexists with antimony (Sb) due to their similar properties, and elevated As levels are detected near Sb mining sites. Photochemical techniques are important for the treatment of organic and inorganic contaminants, including As and Sb. In this study, the influence of widely distributed low-molecular-weight organic acids (LMWOAs) in the environment or frequently used in heavy-metal remediation techniques on the photochemical reduction of As(III)/As(V) was investigated in the presence of Sb(III)/Sb(V).

A novel preparation method for black rice wine (beer, Huangjiu and sweet wine) and its association with a core nutrient-metabolite network.

Black rice is rich in nutrients and physiologically active substances. In this study, we developed a new method for brewing black rice beer, Chinese rice wine (Huangjiu), and sweet wine by adding black rice and constructed a nutritional metabolism network. The results revealed that the total antioxidant capacity and the contents of flavonoids, total phenols, vitamins, mineral elements and other nutritional and physiologically active substances and nutrients in black rice wine significantly increased.

Functional connectivity network based on scalp electroencephalogram in juvenile myoclonic epilepsy.

Background: Advances in neuroimaging technology have identified epilepsy as a disorder of brain network dysfunction. Previous research has confirmed alterations in the brain functional network of patients with juvenile myoclonic epilepsy (JME). However, studies investigating brain network changes in JME patients under different intervention conditions remain scarce.

Association between UBQLN Family Gene and Systemic Lupus Erythematosus: A Case Control Study.

Objective: Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disorder. This study aims to investigate the association between UBQLN family genes and the systemic lupus erythematosus (SLE).

Methods: In the present study, plasma levels of UBQLN family genes were evaluated in 113 SLE patients and 115 healthy controls.

An intelligent tactile imaging-recognition sensor system enabled a methoxynitrobenzene-salicylaldehyde fluorescent material.

Tactile sensors utilizing functional materials decode surface textures for object recognition. Herein, we engineer a donor-acceptor fluorescent material, MNIMP, that synergizes aggregation-induced emission (AIE) and twisted intramolecular charge transfer (TICT) mechanisms. Contact-induced nanoflake assembly on the MNIMP film triggers fluorescence amplification mediated by the combined AIE and TICT effects, through which the surface morphology of textured objects can be accurately visualized as fluorescent patterns.

Vascularized iliac crest flap with double musculocutaneous skin islands for reconstruction of composite buccal and mandibular defects after gingival squamous cell carcinoma resection: A case report.

Mandibular gingival squamous cell carcinoma (GSCC) is a challenging malignancy often requiring extensive surgical resection and complex reconstruction. This study presents a 69-year-old male with left mandibular GSCC, treated with segmental mandibulectomy and selective neck dissection, followed by reconstruction using a deep circumflex iliac artery (DCIA) vascularized iliac crest flap with double musculocutaneous skin islands. Preoperative virtual surgical planning and 3D-printed surgical guides facilitated precise osteotomy and optimal flap design.

Impact of Brown Rice as Adjunct on Beer Brewing.

The utilization of alternative cereals for brewing beer has garnered significant interest in contemporary times. The utilization of alternative cereals as adjuncts has great potential for creating novel beer flavour profiles and cost savings. Brown rice (BR) is the unpolished rice grain that retains its outer layer post-hulling and is nutritionally superior to polished rice (PR).

: A Neurodevelopmental Factor Regulating Cell Metabolism in the Tumor Microenvironment.

The Fragile X Mental Retardation 1 () gene is well-known for its role in Fragile X syndrome, a neurodevelopmental disorder, but emerging evidence suggests its involvement in regulating cellular metabolism, with implications for cancer biology. encodes the Fragile X mental retardation protein (FMRP), an RNA-binding protein that controls various cellular processes, including translation, synaptic plasticity, and RNA metabolism. Recent studies have uncovered novel links between , metabolic regulation, and tumorigenesis.

Mental illness-related stigma and its associated factors among primary health care professionals in rural China.

Background: Primary health care professionals (PHCPs) play a key role in the workforce of community mental health services in rural China. This study aimed to explore the mental illness-related stigma and its associated factors among PHCPs in rural communities.

Methods: This study collected the data from 247 PHCPs in 10 township health service centers in Xinjin District, Chengdu, Sichuan Province, China from November to December 2023.

Molecular Characteristics and Phenotypical Analysis of Carbapenem-Resistant in the Lüliang Region, Shanxi Province.

Introduction: To explore the resistance characteristics and molecular features of carbapenem-resistant () isolates prevalent in the Lüliang region, 81 isolates were collected from three hospitals in the Lüliang region, Shanxi Province.

Methods: The resistance of these isolates to 11 antibiotics was determined using the disk diffusion method, and antimicrobial resistance encoding genes and virulence genes were detected by PCR. The mucoid phenotype of the isolates was examined via the string test, and bacterial biofilm formation ability was measured using the crystal violet staining method.

Total Flavonoids of Engelhardia roxburghiana Wall. Attenuated Nonalcoholic Fatty Liver Disease by Regulating Lipid Metabolism and Inflammation Through Targeting SREBP1 and PPARα.

This research delved into the effects and mechanisms of total flavonoids of Engelhardia roxburghiana Wall. (TFER) on nonalcoholic fatty liver disease (NAFLD). High-fat diet-induced NAFLD rat models and palmitic acid-induced HepG2 cell models were employed.

Clinical and Genetic Factors Associated with Intraoperative Minimum Alveolar Concentration Ratio: A Single-center Retrospective Cohort and Genome-wide Association Study.

Background: Minimum alveolar concentration (MAC) is a standard dosing metric for general anesthesia. Although influences of MAC have been identified in controlled studies, the determinants of clinical delivery of MAC ratio are largely unknown. To address this knowledge gap, the authors performed this single-center retrospective study.

Iliac crest free flap versus fibula free flap for mandibular reconstruction: Cost-effectiveness analysis in a Chinese population.

Mandibular reconstruction is vital in oral and maxillofacial surgery to restore aesthetics and function after tumor resection or trauma. The iliac crest-free flap (ICFF) and fibula-free flap (FFF) are widely used, but their cost-effectiveness and impact on quality of life (QoL) are debated. A retrospective study of 142 patients (2012-2018) compared ICFF and FFF, analyzing direct costs (surgical, medication, hospitalization) and indirect costs (surgery duration, hospital stay).