Targeted urinary metabolomics combined with machine learning to identify biomarkers related to central carbon metabolism for IBD.

Introduction: Inflammatory bowel disease (IBD), comprising Crohn's disease (CD) and ulcerative colitis (UC), is a chronic and relapsing inflammatory disorder of the gastrointestinal tract. Current diagnostic approaches are invasive, costly, and time-consuming, underscoring the need for non-invasive, accurate diagnostic methods.

Methods: We conducted a targeted metabolomic analysis of 49 metabolites related to central carbon metabolism in urinary samples from individuals with IBD and control group.

A critical review of generation regularities and regulatory strategies of reactive species in peracetic acid activation: classification of active methods, influencing factors, and evaluation methods.

Water pollution from various emerging pollutants (EPs) poses a potential threat to human health and ecological security. Peracetic acid-based advanced oxidation processes (PAA-AOPs) have emerged as a promising technology for eliminating EPs owing to susceptible activation capability, selective oxidation, and high efficiency for decontamination. However, the lack of systematic understanding of reactive species (RSs) generation mechanisms and regulation strategies hinders their targeted application.

Novel ECOMF system: In situ ferrate generation for contaminant removal and fouling mitigation in brackish water desalination.

The persistent presence of contaminants of emerging concern (CECs) and severe membrane fouling remain critical challenges in the desalination of micro-polluted brackish water, necessitating innovative solutions for brackish water treatment. This study proposes, for the first time, an electrocoagulation-electrocatalytic oxidation-membrane filtration (ECOMF) system based on in-situ generation of ferrate (Fe), which synergistically addresses membrane fouling and CECs removal. By integrating electrocoagulation (EC), electrocatalytic oxidation (EO), and conductive membrane filtration, the system enables simultaneous oxidation, coagulation, and fouling mitigation.

Enhancing hydrolysis acidification process for coal gasification wastewater treatment through micro-aeration strategy: The role of micro-oxygen environment.

Micro-aeration has been regarded as an efficient, economical, and promising strategy for enhancing the anaerobic hydrolysis acidification (HA) of toxic and recalcitrant organics. However, the available information on both its application in actual refractory industrial wastewater and the molecular-level interrelated mechanisms of functional genes and functional enzymes in pollutants degradation is limited. Herein, micro-aeration HA was exploited to investigate the degradation performance of actual coal gasification wastewater under various dissolved oxygen (DO) concentrations.

Real "zero energy consumption" for efficient antibiotics degradation by floating photocatalysis: modeling, degradation pathway and toxicity assessment.

Floating photocatalysis is a real "zero energy consumption" and practicable green water treatment technology because of no need for reactor and pump, and direct contact with sunlight to produce more free radicals compared to traditional immersion photocatalysts. To realize "green" and "safe" practical application, efficiency for different water quality bodies and toxicity assessment of degradation products are key factors. In this work, an economic and efficient floating photocatalyst Bi doped P25-TiO (Bi@P25)/expanded perlite (EP), named BTEP was successfully constructed, exhibiting stronger visible light absorption and faster photogenerated carriers separation ability due to Bi doping and formation of Bi-O-Si bond.

Generation of an anti-CD5 CAR knock-in human induced pluripotent stem cell line using CRISPR/Cas9 technology.

Theanti-CD5 chimeric antigen receptor (CAR)isa genetically engineered immune cell therapydevelopedto targetCD5-associatedhematologic malignancies,such asT-cell lymphoma and leukemia.UsingCRISPR/Cas9-mediated gene targeting, wegeneratedananti-CD5 CAR knock-in human induced pluripotent stem cell (iPSC) linethat stably expresses the CAR constructand is detectable via FLAG tag and GFP markers. Thisengineeredcell linemaintainsstem cell morphology,displaysa normal karyotype, andexhibits robustexpression ofpluripotency markers while retaining differentiation potential.

Reshaping the gut microbiota: A novel oppinion of Eucommiae cortex polysaccharide alleviate learning and memory impairments in Alzheimer's disease.

Background: Alzheimer's disease (AD), which is a chronic neurodegenerative disorder, is marked by the progressive deteriorations in learning and memory capabilities. The microbiota-gut-brain axis has come to be regarded as a crucial element in relation to the pathogenesis as well as the treatment of AD. Eucommiae cortex polysaccharides (EPs), being among the most plentiful substances present in the Eucommiae cortex, show the potential to exert immunomodulatory and neuroprotective function.

Pharmacological modulation of mitochondrial function as novel strategies for treating intestinal inflammatory diseases and colorectal cancer.

Inflammatory bowel disease (IBD) is a chronic and recurrent intestinal disease, and has become a major global health issue. Individuals with IBD face an elevated risk of developing colorectal cancer (CRC), and recent studies have indicated that mitochondrial dysfunction plays a pivotal role in the pathogenesis of both IBD and CRC. This review covers the pathogenesis of IBD and CRC, focusing on mitochondrial dysfunction, and explores pharmacological targets and strategies for addressing both conditions by modulating mitochondrial function.

Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies.

Neutrophils are essential innate immune cells with unusual anti-microbial properties while dysfunctions of neutrophils lead to severe health problems such as lethal infections. Generation of neutrophils from human induced pluripotent stem cells (hiPSCs) is highly promising to produce off-the-shelf neutrophils for transfusion therapies. However, the anti-microbial potencies of hiPSCs derived neutrophils (iNEUs) remain less documented.

an allelic variant of as high-quality silage maize genetic resource.

Introduction: Stem brittleness significantly affects both yield and quality of maize.

Methods: Using phenotypic identification and sequence analysis, we identified a new brittle stalk maize mutant. Furthermore, we assessed its feeding value by content determination of cellulose, hemicellulose, lignin crude fiber, starch, and protein contents.

A two-transcript classifier model for assessing disease activity in patients with ulcerative colitis: A discovery and validation study.

Aims: We aimed to develop and validate classifier models to assess disease activity in UC patients by evaluating potential classifier genes expression levels.

Methods: The study comprised UC and healthy control participants undergoing colonoscopy. We screened candidate genes (TGF-β1, CEACAM1 and CD177) using Differentially Expressed Genes.

Discovery of Natural Resorcylic Acid Lactones as Novel Potent Copper Ionophores Covalently Targeting PRDX1 to Induce Cuproptosis for Triple-Negative Breast Cancer Therapy.

Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer. Cuproptosis, a novel identified cell death form, is triggered by the direct binding of copper to lipoylated components of the tricarboxylic acid cycle. Identifying new effective drug targets and copper ionophores inducing cuproptosis for TNBC therapy is an urgent clinical need.

Morphology Effect of Puffball Spores on Hemostasis: A Promising Solution for Hemostatic Challenges.

Hemostatic materials play a crucial role in wound healing by promoting blood concentration or releasing procoagulant factors. While hydrophilic hemostatic materials are effective, they may cause excessive blood loss and difficulty removing from the wound. Conversely, hydrophobic hemostatic materials avoid these issues but may hinder blood concentration and the release of procoagulant factors due to their water-repellent nature.

Molecular-dipole oriented universal growth of conjugated polymers into semiconducting single-crystal thin films.

Precise control over crystallinity and morphology of conjugated polymers (CPs) is essential for progressing organic electronics. However, manufacturing single-crystal thin films of CPs presents substantial challenges due to their complex molecular structures, distorted chain conformations, and unbalanced crystallization kinetics. In this work, we demonstrate a universal nanoconfined molecular-dipole orientating strategy to craft high-quality single-crystal thin films for a variety of CPs, spanning from traditional thiophene- and theinothiophene-based homopolymers to diketopyrrolopyrrole- (i.

Mechanisms of RCD-1 pore formation and membrane bending.

Regulator of cell death-1 (RCD-1) governs the heteroallelic expression of RCD-1-1 and RCD-1-2, a pair of fungal gasdermin (GSDM)-like proteins, which prevent cytoplasmic mixing during allorecognition and safeguard against mycoparasitism, genome exploitation, and deleterious cytoplasmic elements (e.g., senescence plasmids) by effecting a form of cytolytic cell death.

Demonstration of high-reconfigurability and low-power strong physical unclonable function empowered by FeFET cycle-to-cycle variation and charge-domain computing.

Physical unclonable functions (PUFs) are of immense potential in authentication scenarios for Internet of Things (IoT) devices. For creditable and lightweight PUF applications, key attributes, including low power, high reconfigurability and large challenge-response pair (CRP) space, are desirable. Here, we report a ferroelectric field-effect transistor (FeFET)-based strong PUF with high reconfigurability and low power, which leverages the FeFET cycle-to-cycle variation throughout the workflow and introduces charge-domain in-memory computing.

Melatonin alleviates heat stress-induced spermatogenesis dysfunction in male dairy goats by regulating arachidonic acid metabolism mediated by remodeling the gut microbiota.

Background: Heat stress (HS) commonly occurring in summer has gradually become a factor threatening the reproductive performance of male dairy goats by reducing their fecundity. Despite the melatonin is applied to relieve HS, it is still unclear whether melatonin protects against reproductive damage induced by HS in dairy goats and how it works. The purpose of the present study is to evaluate the role of melatonin in alleviating HS-induced spermatogenesis dysfunction in male dairy goats and further explore its mechanism.

Transcriptomic analysis of the gonads of (Orthoptera: Acrididae) following infection with .

is an environmentally friendly parasitic predator with promising applications in locust control. In this study, transcriptome sequencing was conducted on gonadal tissues of males and females infected and uninfected with at different developmental stages. A total of 18,635 differentially expressed genes (DEGs) were identified in female ovary tissue transcriptomes, with the highest number of DEGs observed at 1 day post-eclosion (7141).

Modulation of liver metabolism and gut microbiota by Alhagi-honey alleviated heat stress-induced liver damage.

Alhagi-honey (AH) is a well-established traditional ethnic medicine with advantageous effects against diarrhea and headaches. We aimed to explore the preventive effect of AH on liver damage induced by heat stress (HS) and its underlying mechanism. HS models were established by thermostat, and mice were treated at 39 ℃ for 10 h, lasting for 7 days.

Genome-Wide Identification and Analysis of Maize Family Genes in Response to Salt, Heat, and Cold at the Seedling Stage.

DnaJ proteins, also known as HSP40s, play a key role in plant growth and development, and response to environmental stress. However, little comprehensive research has been conducted on the gene family in maize. Here, we identify 91 genes from maize, which are likely distributed in the chloroplast, nucleus, and cytoplasm.

Improving management in gastroesophageal reflux disease through leveraging WeChat platform for mobile health care: A randomized control trial.

Background: Gastroesophageal reflux disease (GERD) refers to a clinical condition characterized by gastric content reflux into the esophagus, causing symptoms like acid regurgitation and heartburn. While patient education is essential for GERD treatment, traditional educational models often struggle to effectively improve treatment outcomes.

Methods: Between January 2021 and April 2022, we enrolled 257 patients and assessed their GERD knowledge.

Identification of Novel Target DCTPP1 for Colorectal Cancer Therapy with the Natural Small-Molecule Inhibitors Regulating Metabolic Reprogramming.

Colorectal cancer (CRC) is one of the most common malignant tumors. Identification of new effective drug targets for CRC and exploration of bioactive small-molecules are clinically urgent. The human dCTP pyrophosphatase 1 (DCTPP1) is a newly identified pyrophosphatase regulating the cellular nucleotide pool but remains unexplored as potential target for CRC treatment.