A cocktail hydrogel promoting the functional interneurons regeneration of human neural progenitor cells for brain injury therapy.

Introduction: Traumatic Brain Injury (TBI) usually leads to substantial mortality and disability among adult populations. Neural progenitor cells (NPCs) transplantation exhibits great potential in TBI treatment. However, the differentiation rate of interneurons is relatively low, largely impeding the therapeutic effects of brain tissue repair.

METTL13 is essential for the survival of acute myeloid leukemia cells by regulating MYC.

Recently, some methyltransferase-like (METTL) proteins have been found to play crucial roles in the development of acute myeloid leukemia (AML) through mediating RNA modifications, such as METTL3/14/16 mediated N-methyladenosine (mA) and METTL1 mediated N-methylguanosine (mG). However, the roles of other METTL proteins in AML progression remain unknown. Here, we examined the expression levels of all METTL members in AML samples and showed that METTL13 was increased in AML and positively correlated with poor prognosis.

HBO1 determines epithelial-mesenchymal transition and promotes immunotherapy resistance in ovarian cancer cells.

Purpose: Epithelial-mesenchymal transition (EMT) plays critical roles in tumor progress and treatment resistance of ovarian cancer (OC), resulting in the most deadly gynecological cancer in women. However, the cell-intrinsic mechanism underlying EMT in OC remains less illuminated.

Method: SKOV3, the OC cell line, was treated with TGF-β to induce EMT or with SB431542, an inhibitor of the TGF-β signaling pathway, to reduce migration.

Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies.

Neutrophils are essential innate immune cells with unusual anti-microbial properties while dysfunctions of neutrophils lead to severe health problems such as lethal infections. Generation of neutrophils from human induced pluripotent stem cells (hiPSCs) is highly promising to produce off-the-shelf neutrophils for transfusion therapies. However, the anti-microbial potencies of hiPSCs derived neutrophils (iNEUs) remain less documented.

METTL3/METTL14 maintain human nucleoli integrity by mediating SUV39H1/H2 degradation.

Nucleoli are fundamentally essential sites for ribosome biogenesis in cells and formed by liquid-liquid phase separation (LLPS) for a multilayer condensate structure. How the nucleoli integrity is maintained remains poorly understood. Here, we reveal that METTL3/METTL14, the typical methyltransferase complex catalyzing N6-methyladnosine (mA) on mRNAs maintain nucleoli integrity in human embryonic stem cells (hESCs).

HBO1 determines SMAD action in pluripotency and mesendoderm specification.

TGF-β signaling family plays an essential role to regulate fate decisions in pluripotency and lineage specification. How the action of TGF-β family signaling is intrinsically executed remains not fully elucidated. Here, we show that HBO1, a MYST histone acetyltransferase (HAT) is an essential cell intrinsic determinant for TGF-β signaling in human embryonic stem cells (hESCs).

Large-scale generation of IL-12 secreting macrophages from human pluripotent stem cells for cancer therapy.

Genetically engineered macrophages (GEMs) have emerged as an appealing strategy to treat cancers, but they are largely impeded by the cell availability and technical challenges in gene transfer. Here, we develop an efficient approach to generate large-scale macrophages from human induced pluripotent stem cells (hiPSCs). Starting with 1 T150 dish of 10 hiPSCs, more than 10 mature macrophages (iMacs) could be generated within 1 month.

Autophagy is essential for human myelopoiesis.

Emergency myelopoiesis (EM) is essential in immune defense against pathogens for rapid replenishing of mature myeloid cells. During the EM process, a rapid cell-cycle switch from the quiescent hematopoietic stem cells (HSCs) to highly proliferative myeloid progenitors (MPs) is critical. How the rapid proliferation of MPs during EM is regulated remains poorly understood.