Proteome and Ubiquitinome Analyses Reveal the Involvement of Ubiquitination in Resistance to Maize Lethal Necrosis.

The co-infection of maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) causes maize lethal necrosis (MLN), which seriously affects the yield and quality of maize. Ubiquitination is one of the most important protein post-translational modifications. However, the role of ubiquitination modification in regulating maize resistance to viral infection remains largely unknown.

Prevalence and Associated Factors of Anxiety and Depression in Lung Cancer Patients.

Purpose: Given the prevalence of anxiety and depression amongst lung cancer patients in China, it is critical to identify potential factors contributing to these symptoms to improve future treatment strategies. However, current research has primarily focused on clinical variables, leaving various sociodemographic factors largely underexplored. Examining these aspects is essential for enhancing clinical interventions and patient care, as sociodemographic factors can significantly influence psychological outcomes.

Clinical features and perinatal outcomes in women with adenomyosis.

Objective: To analyze the correlation between Clinical Features and Perinatal Outcomes in the population of pregnancies with adenomyosis.

Methods: This retrospective study analyzed 413 pregnant women with ultrasonography/MRI-confirmed adenomyosis (diagnosed pre-pregnancy/early pregnancy) who delivered or miscarried at Peking University Third Hospital (2022-2024). Clinical features (dysmenorrhea, metrorrhagia, uterine volume, concurrent endometriosis) were evaluated for associations with delivery mode, gestational age, postpartum blood loss, premature rupture of membranes (PROM), operation time of cesarean section, placenta accreta, placenta previa, gestational hypertension.

A trifunctional coating for biofilm prevention: integrating antifouling, photothermal killing, and quorum sensing interference.

Bacterial biofilm-associated infections pose a significant challenge in biomedical fields, especially in the context of medical devices and implants, where conventional treatments often fail to combat mature biofilms. To overcome this limitation, an antibiofilm coating was developed, incorporating three functional components: carbon nanoparticles derived from candle soot (CS), hydrophilic and pH-responsive copolymer brushes of 2-hydroxyethyl methacrylate (HEMA) and 3-(acrylamido)-phenylboronic acid (APBA), and natural quorum sensing inhibitors (QSIs). The carbon nanostructure of the CS substrate provides inherent photothermal conversion properties, while its high surface area facilitates efficient chemical functionalization.

Camel milk extracellular vesicles-mediated co-delivery of siVEGF/sorafenib enhances the anti-hepatocellular carcinoma activity by dual-effect blocking the biological effects of VEGF.

In view of the combined effect of paracrine and autocrine of VEGF in tumor tissues, this study proposed to use sorafenib to block VEGF signaling pathway, while siRNA was used to interfere with tumor cell autocrine of VEGF, so as to achieve double-effect blocking of VEGF biological effects and further improve anti-tumor efficacy. Here, we constructed a novel co-delivery system (so-cEVs-PPT/siVEGF) based on camel milk-derived extracellular vesicles (cEVs) modified by Plannic 123-polyethylenimine-tLyP-1 transmembrane peptide polymer (PPT) for the delivery of siVEGF and sorafenib. The results showed that so-cEVs-PPT/siVEGF exhibited significant tumor inhibition and low side effects on hepatocellular carcinoma mice, and the combined administration system had stronger anti-tumor effects than siVEGF or sorafenib alone.

Long-Term Exercise Mitigates Energy Expenditure and Inflammatory Responses Induced by Sleep Deprivation in Mice.

: Sleep deprivation (SD), defined as the disruption or loss of normal sleep, negatively affects energy metabolism, immune function, and gut microbiota in both humans and animals. Although SD has detrimental effects, it is often unavoidable due to work or study demands. Exercise has been shown to improve sleep quality, regulate metabolism, and enhance immune function.

Bibliometric analysis of the interplay between Alzheimer's disease and type 2 diabetes mellitus: Trends, hotspots, and emerging research areas.

BackgroundType 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD) are major global health concerns, characterized by rising prevalence, high healthcare costs, and significant reductions in patients' quality of life. Emerging evidence suggests that individuals with T2DM have nearly double the risk of developing AD, potentially due to overlapping mechanisms such as insulin resistance, oxidative stress, and neuroinflammation.ObjectiveThis study aims to systematically explore the evolving research landscape at the intersection of T2DM and AD over the past two decades, identifying major contributors, shifting research focuses, and emerging trends to inform future investigations and therapeutic development.

USP30 Aggravating the Malignant Progression of Breast Cancer and Its Resistance to Tamoxifen by Inhibiting the Ubiquitination of TOMM40.

Breast cancer (BC) is the most common malignancy among women, with high incidence and mortality rates globally. Translocase of outer mitochondrial membrane 40 (TOMM40) has also been identified as an important prognostic biomarker for BC. Meanwhile, the ubiquitin-specific protease 30 (USP30) has also been shown to promote BC progression.

Predicting prognosis of patients with triple‑negative breast cancer undergoing neoadjuvant chemotherapy based on inflammatory status at different time points: A propensity score matching analysis.

Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer with limited targeted treatment options, making the identification of reliable prognostic markers crucial for improving patient outcomes. The present study aimed to assess the predictive ability of pre-chemotherapy and pre-surgery inflammatory status on the prognosis of patients with TNBC undergoing neoadjuvant therapy. A total of 422 patients with TNBC who received neoadjuvant chemotherapy at the Inner Mongolia People's Hospital between January 2017 and December 2022 were selected for analysis.

A review of MicroRNAs and flavonoids: New insights into plant secondary metabolism.

Flavonoids, essential plant secondary metabolites, play crucial roles in growth regulation, stress responses, and applications in medicine, agriculture, and industry. However, the complexity of their biosynthetic pathways and regulatory networks poses challenges for industrial-scale production. MicroRNAs (miRNAs), as pivotal post-transcriptional regulators, play significant roles in fine-tuning flavonoid metabolism by targeting key enzyme genes and transcription factors.

Suppression of astrocyte elevated gene-1 protects against light-induced photoreceptor apoptosis and inflammation in retina.

Purpose: To research the function of astrocyte elevated gene-1 (AEG-1) in light-induced retinal degeneration.

Methods: The retinas of BALB/c mice and 661W cells damage were induced by exposure to light; Lipopolysaccharide (LPS) was used to stimulate BV2 cells. AEG-1 siRNA transfection was used to inhibit AEG-1.

Designing interfacially stable Na-ion polymer electrolytes with tailored local solvation structures.

Azodicarbonamide (ADA) is selected as an additive to the polymer electrolyte (PE) to improve the stability of the NaFeMnNiO cathode. ADA can capture hydrogen from the polymer and induce local structures, enhancing the ionic conductivity of the PE. Moreover, the dehydrogenated ADA can bond to Fe ions, preventing the PE from decomposing.

Prolonged Exposure to Environmentally Relevant Concentrations of Chlorine Induces Heritable Antimicrobial Resistance in Disinfection Residual .

Chlorination, a crucial step in pathogen control, raises concerns due to the potential residual chlorine presence during water treatment and sanitation. However, the consequences of prolonged exposure to environmentally relevant chlorine concentrations on antimicrobial resistance (AMR) evolution and its driving mechanism in bacteria remain unclear. Therefore, this study utilized a combination of phenotypic and genotypic analyses, revealing that chlorination at concentrations of 0.

Butyrate-engineered yeast activates Nppa and Sgcg genes and reduces radiation-induced heart damage via the gut-heart axis.

Radiotherapy is a method of treating cancer through radiation aimed at killing cancer cells or inhibiting their growth. However, radiotherapy has numerous side effects because it kills tumors while causing damage to normal cells or tissues. The literature shows that radiation can cause damage to heart tissue.

Predictive coding compressive sensing optical coherence tomography hardware implementation.

Compressed sensing (CS) is an approach that enables comprehensive imaging by reducing both imaging time and data density, and is a theory that enables undersampling far below the Nyquist sampling rate and guarantees high-accuracy image recovery. Prior efforts in the literature have focused on demonstrations of synthetic undersampling and reconstructions enabled by compressed sensing. In this paper, we demonstrate the first physical, hardware-based sub-Nyquist sampling with a galvanometer-based OCT system with subsequent reconstruction enabled by compressed sensing.

Propionate-producing engineered probiotics ameliorated murine ulcerative colitis by restoring anti-inflammatory macrophage via the GPR43/HDAC1/IL-10 axis.

Inflammatory bowel disease (IBD) is a chronic and unspecific inflammatory disorder of the gastrointestinal tract, and current treatment options often fail to maintain long-term remission. Studies have shown that propionate level is reduced in fecal samples from patients with IBD. Propionate can ameliorate IBD through intestinal epithelial cells and immune regulation, but its effects on the inflammatory microenvironment and macrophage differentiation have not been widely studied.

Cordycepin enhances the Anticancer efficacy of PD-L1 blockade by modulating the tumor microenvironment of colon cancer.

Background: PD-L1 blockade has been found to be effective in treating multiple malignancies. Combined therapy is proposed to provide better therapeutic effects. Cordycepin, a prominent bioactive compound found in cordyceps, can inhibit the development of various cancers.

N-Methyladenosine RNA Modification Regulates Maize Resistance to Maize Chlorotic Mottle Virus Infection.

Maize chlorotic mottle virus (MCMV) is one of the main viruses causing significant losses in maize. N-methyladenosine (mA) RNA modification has been proven to play important regulatory roles in plant development and stress response. In this study, we found that MCMV infection significantly up-regulated the mA level in maize, and methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were performed to investigate the distribution of mA modified peaks and gene expression patterns in MCMV-infected maize plants.

Transcriptome, histology, and enzyme activities analysis of liver in Phoxinus lagowskii to the low temperature stress and recovery.

Assessing the response and resilience of fish to low temperatures over different time scales can provide valuable insights into their mechanisms of adaptation to cold conditions. Farmed Amur minnows (Phoxinus lagowskii) frequently encounter low temperatures, especially during winter. However, the specific responses of P.

Association of CD8TILs co-expressing granzyme A and interferon-γ with colon cancer cells in the tumor microenvironment.

CD8T cells secreting granzyme A (GZMA) can induce pyroptosis in tumor cells by effectively cleaving gasdermin B (GSDMB), which is stimulated by interferon-γ (IFN-γ). However, the interaction between GZMA-expressing CD8T cells and GSDMB-expressing tumor cells in colon cancer remains poorly understood. Our research employed multi-color immunohistochemistry (mIHC) staining and integrated clinical data to explore the spatial distribution and clinical relevance of GZMA- and IFN-γ-expressing CD8 tumor-infiltrating lymphocytes (TILs), as well as GSDMB-expressing CK cells, within the tumor microenvironment (TME) of human colon cancer samples.

Protective effect of melatonin against metabolic disorders and neuropsychiatric injuries in type 2 diabetes mellitus mice.

Background: Type 2 diabetes mellitus (T2DM) is a metabolic disease characterized by hyperglycemia and progressive cognitive dysfunction, and our clinical investigation revealed that the plasma concentration of melatonin (Mlt) decreased and was closely related to cognition in T2DM patients. However, although many studies have suggested that Mlt has a certain protective effect on glucose and lipid metabolism disorders and neuropsychiatric injury, the underlying mechanism of Mlt against T2DM-related metabolic and cognitive impairments remains unclear.

Purpose: The aim of the present study was to investigate the therapeutic effect of Mlt on metabolic disorders and Alzheimer's disease (AD)-like neuropsychiatric injuries in T2DM mice and to explore the possible underlying molecular mechanism involved.