Numerical study on the effect of pile shape and loading direction on the lateral response of displacement pile in sand.

Several finite element numerical simulations were conducted on laterally loaded piles in Geba sand to investigate how pile shape, loading direction, and pile length impact lateral load transfer curves and pile-soil interaction. The stress-strain behavior of Geba sand was defined using the hypoplastic constitutive model within the finite element software via the UMAT subroutine. The accuracy of the finite element model was confirmed through a comparison with the centrifuge test findings from prior research.

Overexpression of the ABA-responsive transcription factor PtrMYBH alters shoot apical meristem, xylem, root, and leaf development in Populus.

Abscisic acid (ABA) mediates stress responses and growth regulation in plants, but the roles of ABA-responsive transcription factors (TFs) in Populus development remain poorly characterized. Here, we identified an ABA-upregulated TF and investigated its function through overexpression in transgenic poplar. The PtrMYBH is upregulated during ABA treatment, and its overexpression in transgenic poplar leads to leaf malformations, including reduced size and curling, with severity correlating with PtrMYBH-overexpression levels in three independent transgenic lines.

ICAM-1-mediated Src signaling pathway plays a pivotal role in encephalomyocarditis virus entry.

Encephalomyocarditis virus (EMCV), a potential zoonotic pathogen with a broad host range, has been reported to enter cells via caveolin-mediated endocytosis (CavME), a process initiated by caveolae detachment from the plasma membrane. However, the molecular mechanism underlying EMCV's cellular entry remains incompletely characterized. Here, we identified for the first time that the overexpression of Src kinase and Fyn kinase individually enhanced the replication and proliferation of EMCV.

A candidate multi-epitopes vaccine against encephalomyocarditis virus confers protection in mice.

Encephalomyocarditis virus (EMCV) is a widely distributed RNA virus that causes diseases in animals with zoonotic potential. Infection with this pathogen in animals, especially in pigs, can lead to encephalitis, myocarditis, and reproductive disorders. The virus causes a febrile disease in humans and is thus not only a potential threat to the swine industry but also to human health and society.

Linker histone H1.3 promotes IFN-β production targeting MDA5 signaling to inhibit EMCV infection.

Multiple core histones play pivotal roles in viral infection process, as evidenced in influenza virus and other viruses. Recent findings indicate that linker histone H1.2 regulates the interferon signaling pathway to modulate influenza and EMCV infections, while H1.

Formation Mechanism and Antibacterial Activity of Natural Antimicrobial Lysozyme with Antibiotics Doxycycline and Tigecycline.

The combination of lysozyme with antibiotics has emerged as a promising strategy to combat bacterial resistance; however, the lack of mechanistic understanding regarding their molecular-level interactions remains a critical knowledge gap, hindering the rational design of optimized synergistic therapies. Through integrated spectroscopic techniques (FTIR, CD, UV-vis, fluorescence), thermodynamic analyses, molecular docking, and 200-ns molecular dynamics simulation, we established that doxycycline/tigecycline bind lysozyme via moderate-affinity hydrophobic interactions (K ∼ 10 L mol) but with distinct binding patterns-tigecycline forms additional hydrogen bonds through its glycylamino side chain, yielding higher stability and lower free drug fraction (f) compare to doxycycline. Additionally, doxycycline and tigecycline have a relatively mild effect on lysozyme conformation, but doxycycline has a greater impact on lysozyme conformation than tigecycline.

Wide-Temperature Electrolyte Design via Cation-Anion Solvation Engineering for 4.6 V Lithium-Ion Batteries.

Conventional lithium-ion batteries (LIBs) employing ethylene carbonate (EC)-based electrolytes and thermally unstable LiPF face dual challenges: sluggish Li-ion transport at low temperatures (≤-20 °C) and severe decomposition at elevated temperatures (≥45 °C). Herein, a synergistic cation-anion solvation engineering strategy is presented for wide-temperature electrolytes, combining EC-free carbonate solvents with a thermally stable ternary lithium salt system. By fine-tuning solvent-salt interactions, the designed electrolyte exhibits facilitated desolvation kinetics and superior ionic conductivity under subzero temperatures (0.

Encephalomyocarditis virus non-structural protein 2C induces the degradation of NDP52 autophagy protein to promote its own survival.

EMCV is a significant zoonotic pathogen that causes severe encephalitis and myocarditis, particularly in pigs, posing substantial economic and public health challenges. Nuclear dot protein (NDP) 52 is an important autophagy adaptor protein known to target microbial pathogens, including viruses into autophagosomes to facilitate the selective autophagy process. Here, we investigated the interaction between EMCV and NDP52.

The interaction mechanism of papain and bromelain with apigenin and luteolin in binary and ternary systems.

In this study, the interaction mechanism of papain and bromelain with apigenin and luteolin in binary and ternary systems was compared and analyzed by spectroscopic methods, molecular docking, and molecular dynamics simulation. Additionally, the effects of apigenin and/or luteolin on the conformational changes and enzymatic activity of papain and bromelain were investigated. The results show that apigenin and/or luteolin exhibit a moderate binding affinity with papain/bromelain, with the binding constants K in the range of 10-10 L mol.

Aqueous high-energy-density capacitor with 2 V output voltage for alternating current line filtering.

High-energy-density aqueous filtering capacitors are essential for the conversion of alternating current to direct current in contemporary integrated circuits. Yet their energy density is limited by the 1.23 V decomposition voltage of water.

Extracellular vesicles engineered to directly target encephalomyocarditis virus ameliorates multi-organ viremia in a lethal infection model.

The outbreak and prevalence of encephalomyocarditis virus (EMCV) causes significant global mortality and morbidity to the pig industry. Though the current and most effective approach to control EMCV outbreak are done through inactivated vaccines, we have yet to see an effective antiviral agent that directly targets EMCV. Here, we present a molecular therapy consisting of extracellular vesicles (EVs) decorated with EMCV-specific single-chain variable fragment (scFv), engineered on the external loop of the EVS transmembrane domain CD63.

MicroRNA-155 rs767649 polymorphism is associated with susceptibility to peri-implantitis.

Objective: To examine how the miR-155 rs767649 polymorphism affects miR-155 expression and to investigate its association with peri-implantitis susceptibility.

Design: One hundred and eighty-seven peri-implantitis patients and 196 healthy implant subjects were enrolled. The expression level of miR-155 in the subjects' serum was tested using qRT-PCR.

Porcine epidemic diarrhea virus nsp14 inhibited IFN-Ⅰ production by targeting RIG-I for degradation.

Porcine epidemic diarrhea virus (PEDV) is enteropathogenic coronavirus, and mainly damages intestines, causing diarrhea, vomiting, anorexia, and depression. PEDV highly pathogenic strains spread rapidly and pose significant economic and public health concerns in our country. After virus invasion, RIG-I detects viral double-stranded RNA to activate antiviral innate immunity, inducing IFN responses.

Functionalized UiO-66 induces shallow electron traps in heterojunctions with InN for enhanced photocathodic water splitting.

Indium nitride (InN) exhibited significant potential as a photoelectrode material for photoelectrochemical (PEC) water splitting, attributed to its superior light absorption, high electron mobility, and direct bandgap. However, its practical application was constrained by rapid carrier recombination occurring within the bulk and at the surface. To address these limitations, researchers developed InN/UiO-66 heterojunction photoelectrodes, which markedly enhanced PEC water splitting for hydrogen production.

Determination of the appropriate extraction method for bound aroma compounds from strawberry and analysis of aroma substances in strawberry fruits of different varieties and developmental stages.

Strawberries are rich in unique aroma substances, which include bound and free aroma compounds. Unlike the free aroma, the appropriate extraction and analytical methods for bound aroma compounds in strawberries remain unclear. In the present study, we compared three extraction methods for bound aroma compounds of strawberries and performed the single factor experiment for optimizing the hydrolysis method, process parameters, and response surface analysis.

Probing the interaction mechanism of tigecycline with γ-globulin and hemoglobin in the absence and presence of amikacin.

The interaction mechanism of tigecycline with γ-globulin and hemoglobin in the absence and presence of amikacin was investigated through multipectral, molecular docking and molecular dynamics simulation. The results show that tigecycline and γ-globulin/hemoglobin forms a ground state complex without or with amikacin. The presence of amikacin slightly increases the binding constant of tigecycline to γ-globulin/hemoglobin, but all are of moderate binding affinity, at 10 L mol.

EMCV VP2 degrades IFI16 through Caspase-dependent apoptosis to evade IFI16-STING pathway.

Interferon (IFN)-γ inducible protein 16 (IFI16), a key DNA sensor, triggers downstream STING-dependent type I interferon (IFN-I) production and antiviral immunity. However, how the IFI16-STING signaling pathway is regulated by EMCV infection is still not well elucidated. In this study, we investigated the interaction between IFI16 and EMCV.

Comparative analysis of the interaction mechanism of γ-globulin and hemoglobin with spherical and rod-shaped gold nanoparticles.

The interaction mechanism of spherical gold nanoparticles (AuNPs) and rod-shaped gold nanoparticles (AuNRs) with γ-globulin and hemoglobin was comprehensively and comparatively analyzed. γ-Globulin and hemoglobin have high affinity with AuNPs, and with two different types of binding sites on AuNRs surface. Except hemoglobin interaction with the first binding site of AuNRs, the interaction between γ-globulin/hemoglobin and AuNPs/AuNRs is the spontaneous, endothermic and entropy-driven process, and hydrophobic interaction plays a dominant role.

Acetylation of TIR domains in the TLR4-Mal-MyD88 complex regulates immune responses in sepsis.

Activation of the Toll-like receptor 4 (TLR4) by bacterial endotoxins in macrophages plays a crucial role in the pathogenesis of sepsis. However, the mechanism underlying TLR4 activation in macrophages is still not fully understood. Here, we reveal that upon lipopolysaccharide (LPS) stimulation, lysine acetyltransferase CBP is recruited to the TLR4 signalosome complex leading to increased acetylation of the TIR domains of the TLR4 signalosome.

Effect of Polymer Encapsulation on the Mechanoluminescence of Mn-Doped CaZnOS.

Rare earth and transition metal ion-doped CaZnOS has garnered significant attention for its exceptional mechanoluminescence (ML) performance under mild mechanical stimuli and its capability for multicolor emissions. Since powdered phosphors are not directly usable, they require encapsulation within with polymers to create stable structures. This study investigates Mn-doped CaZnOS (CaZnOS:Mn) as the ML phosphor, optimizing its performance by varying the Mn content, resulting in bright orange-red emissions from the d-d transitions of the Mn activator.

Mesoporous polydopamine/copper sulfide hybrid nanocomposite for highly efficient NIR-triggered bacterial inactivation.

Polydopamine has gained considerable attention in the biomaterial domain owing to its excellent biocompatibility, antioxidant activity, photothermal effect and adhesion property. Herein, copper sulfide (CuS) wrapped in mesoporous polydopamine (MPDA) was synthesized through in-situ polymerization, followed by the surface modification with cationic polyethyleneimine (PEI). The mussel-inspired MPDA matrix successfully prevented the oxidation and agglomeration of CuS nanoparticles, and regulated the release of copper ions and reactive oxygen species (ROS) levels.