Salicylic acid reduces ELF3 phase separation and suppresses thermomorphogenic growth in Arabidopsis.

Salicylic acid (SA), a long-characterized defense hormone, is increasingly recognized for its roles in plant growth and development. However, its involvement in mediating plant growth responses to environmental cues remains less understood. Here, we show that SA negatively affects thermomorphogenic growth in Arabidopsis thaliana.

Optimized thermoelectric properties of SnSe through the joint strategies of Sn-content fine-tuning and CuAgSe alloying.

Polycrystalline SnSe has been widely explored due to its relatively low thermal conductivity. Yet its thermoelectric performance is limited due to its poor electrical conductivity. In this work, polycrystalline SnSe alloyed with different contents of CuAgSe were fabricated by a high-energy balling and melting method, combined with spark plasma sintering.

Peiyuan Ningshen-ointment alleviates learning and memory impairment induced by sleep deprivation through activating the cAMP/PKA signaling pathway.

Background: Sleep Deprivation (SD) seriously impacts the brain's learning and memory functioning, and the treatment methods and their efficacy are limited. PYNS ointment (Peiyuan Ningshen-ointment) is an herbal ointment consisting of eleven Chinese herbs, which have been proven to have positive therapeutic effects on learning and memory impairment. However, its mechanism of action is still unclear.

Tunable Hierarchical Pore Structure of Nickel-Cobalt Bimetallic Organic Framework Materials for High-Performance Supercapacitor.

In this work, a series of Ni/Co-MOFs with high specific capacitances were synthesized as anode materials using a one-step hydrothermal reaction method. NaOH in different amounts (3, 4, 5, and 6 mmol) was added during the synthesis to tune the pore structure of Ni/Co-MOFs. It was found that the Ni/Co-MOF-3 with a NaOH amount of 5 mmol exhibits the largest specific surface area and pore volume, which provides more active sites for the electrochemical reaction and facilitates ion diffusion at the interface of the electrolyte solution/active material, thus increasing the capacitance of the electrode material.

Shuhe granule for insomnia: study protocol for a double-blind, randomized, placebo-controlled trial.

Introduction: Modern medical treatment of insomnia is often associated with issues like addiction, drug resistance, and a high risk of relapse post drug withdrawal. To tackle these challenges, the Chinese medicine formula Shuhe granule (SHG) has been employed in insomnia treatment at Guangdong Provincial Hospital of Chinese Medicine. Despite this, there is currently a lack of reliable evidence from evidence-based trials to support the widespread use of SHG in insomnia treatment.

Extremely Low Lattice Thermal Conductivity and Significantly Enhanced Near-Room-Temperature Thermoelectric Performance in α-CuSe through the Incorporation of Porous Carbon.

In this work, a series of CuSe/ wt % porous carbon (PC) ( = 0, 0.2, 0.4, 0.

Unraveling the Emerging Photocatalytic, Thermoelectric, and Topological Properties of Intercalated Architecture MZX (M = Ga and In; Z = Si, Ge and Sn; X = S, Se, and Te) Monolayers.

The continuous advancements in studying two-dimensional (2D) materials pave the way for groundbreaking innovations across various industries. In this study, by employing density functional theory calculations, we comprehensively elucidate the electronic structures of MZX (M = Ga and In; Z = Si, Ge, and Sn; X = S, Se, and Te) monolayers for their applications in photocatalytic, thermoelectric, and spintronic fields. Interestingly, GaSiS, GaSiSe, InSiS, and InSiSe monolayers are identified to be efficient photocatalysts for overall water splitting with band gaps close to 2.

Balanced High Thermoelectric Performance in n-Type and p-Type CuAgSe Realized through Vacancy Manipulation.

As a liquid-like material, CuAgSe has high carrier mobility and ultralow lattice thermal conductivity. It undergoes an n-p conduction-type transition during β- to α-phase transition with increasing temperature. Moreover, optimization of the thermoelectric performance of CuAgSe is rather difficult, owing to the two-carrier conduction in this material.

Withdrawn: MiR-101-3p inhibits MTX-mediated HMGB1 release and reduces recruitment and M1-like polarization of macrophages through the UBE2D1/KAT2B pathway.

The above article, published online on 5 December 2022, on Wiley Online Library (https://onlinelibrary.wiley.com/doi/abs/10.

A Rapid PCR-Free Next-Generation Sequencing Method for the Detection of Copy Number Variations in Prenatal Samples.

Next-generation sequencing (NGS) is emerging as a new method for the detection of clinically significant copy number variants (CNVs). In this study, we developed and validated rapid CNV-sequencing (rCNV-seq) for clinical application in prenatal diagnosis. Low-pass whole-genome sequencing was performed on PCR libraries prepared from amniocyte genomic DNA.

Topological Abnormalities of Functional Brain Network in Early-Stage Parkinson's Disease Patients With Mild Cognitive Impairment.

Recent studies have demonstrated structural and functional alterations in Parkinson's disease (PD) with mild cognitive impairment (MCI). However, the topological patterns of functional brain networks in newly diagnosed PD patients with MCI are unclear so far. In this study, we used functional magnetic resonance imaging (fMRI) and graph theory approaches to explore the functional brain network in 45 PD patients with MCI (PD-MCI), 22 PD patients without MCI (PD-nMCI), and 18 healthy controls (HC).

Evaluating the Potential of T Cell Receptor Repertoires in Predicting the Prognosis of Resectable Non-Small Cell Lung Cancers.

For resectable cancer patients, a method that could precisely predict the risk of postoperative recurrence would be crucial for guiding adjuvant treatment. Since T cell receptor (TCR) repertoires had been shown to be closely related to the dynamics of cancers, here we enrolled a cohort of patients to evaluate the potential of TCR repertoires in predicting the prognosis of resectable non-small cell lung cancers. Specifically, TCRβ repertoires were analyzed in surgical tumor tissues and matched adjacent non-tumor tissues from 39 patients enrolled with resectable non-small cell lung cancer, through target enrichment and high-throughput sequencing.

T Cell Receptor Repertoire Sequencing.

The status of T cell receptors (TCRs) repertoire is associated with the occurrence and progress of various diseases and can be used in monitoring the immune responses, predicting the prognosis of disease and other medical fields. High-throughput sequencing promotes the studying in TCR repertoire. The chapter focuses on the whole process of TCR profiling, including DNA extraction, library construction, high-throughput sequencing, and how to analyze data.

Male mammary gland development after apatinib therapy in advanced gastric cancer: A case report.

Rationale: Most gastric cancer patients are diagnosed at mid- to late-stage and lose the chance of radical surgery, medical treatment is especially important to prolong the survival of patients. Apatinib mesylate, which is a small molecule vascular endothelial growth factor receptor 2 tyrosine kinase inhibitor, could be used as antiangiogenesis therapy for gastric cancer.

Patient Concerns: A 67-year-old man sought medical care for upper abdominal discomfort.

Biosynthesis of functional polyhydroxyalkanoates by engineered Halomonas bluephagenesis.

Polyhydroxyalkanoates (PHA) have found widespread medical applications due to their biocompatibility and biodegradability, while further chemical modification requires functional groups on PHA. Halomonas bluephagenesis, a non-model halophilic bacterium serving as a chassis for the Next Generation Industrial Biotechnology (NGIB), was successfully engineered to express heterologous PHA synthase (PhaC) and enoyl coenzyme-A hydratase (PhaJ) from Aeromonas hydrophila 4AK4, along with a deletion of its native phaC gene to synthesize the short chain-co-medium chain-length PHA copolymers, namely poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), poly(3-hydroxybutyrate-co-3-hydroxyhex-5-enoate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyhex-5-enoate). After optimizations of the expression cassette and ribosomal binding site combined with introduction of endogenous acyl-CoA synthetase (fadD), the resulting recombinant strain H.

Prevalence of chromosomal abnormalities identified by copy number variation sequencing in high-risk pregnancies, spontaneous abortions, and suspected genetic disorders.

Objective: High-throughput sequencing based on copy number variation (CNV-seq) is commonly used to detect chromosomal abnormalities including aneuploidy. This study provides evidence for the prevalence of chromosomal abnormalities in target populations.

Methods: A total of 160 samples, including 83 high-risk pregnancies, 37 spontaneous abortions, and 40 suspected genetic disorders, were analyzed by CNV-seq.

Engineering Pseudomonas entomophila for synthesis of copolymers with defined fractions of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates.

Polyhydroxyalkanoates (PHA) composed of both short-chain-length (SCL) and medium-chain-length (MCL) monomers (SCL-co-MCL PHA) combine the advantages of high strength and elasticity provided by SCL PHA and MCL PHA, respectively. Synthesis of SCL-co-MCL PHA, namely, copolymers of 3-hydroxybutyrate (3HB) and MCL 3-hydroxyalkanoates (3HA) such as 3-hydroxydecanoate (3HD) and longer chain 3HA, has been a challenge for a long time. This study aims to engineer Pseudomonas entomophila for synthesizing P(3HB-co-MCL 3HA) via weakening its β-oxidation pathway combined with insertion of 3HB synthesis pathway consisting of β-ketothiolase (phaA) and acetoacetyl-CoA reductase (phaB).

Halomonas and Pathway Engineering for Bioplastics Production.

Traditional microbial chassis, including Escherichia coli, Bacillus subtilis, Ralstonia eutropha, and Pseudomonas putida, are grown under neutral pH and mild osmotic pressure for production of chemicals and materials. They tend to be contaminated easily by many microorganisms. To address this issue, next-generation industrial biotechnology employing halophilic Halomonas spp.

Reprogramming Halomonas for industrial production of chemicals.

Halomonas spp. are able to grow under a high salt concentration at alkali pH, they are able to resist contamination by other microbes. Development of Halomonas spp.

Modelling of microbial polyhydroxyalkanoate surface binding protein PhaP for rational mutagenesis.

Phasins are unusual amphiphilic proteins that bind to microbial polyhydroxyalkanoate (PHA) granules in nature and show great potential for various applications in biotechnology and medicine. Despite their remarkable diversity, only the crystal structure of PhaP from Aeromonas hydrophila has been solved to date. Based on the structure of PhaP , homology models of PhaP from Azotobacter sp.

Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate).

Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), short as P(3HB-co-4HB), was successfully produced by engineered Halomonas bluephagenesis TD01 grown in glucose and γ-butyrolactone under open non-sterile conditions. Gene orfZ encoding 4HB-CoA transferase of Clostridium kluyveri was integrated into the genome to achieve P(3HB-co-4HB) accumulation comparable to that of strains encoding orfZ on plasmids. Fed-batch cultivations conducted in 1-L and 7-L fermentors, respectively, resulted in over 70g/L cell dry weight (CDW) containing 63% P(3HB-co-12mol% 4HB) after 48h under non-sterile conditions.

Novel T7-like expression systems used for Halomonas.

To engineer non-model organisms, suitable genetic parts must be available. However, biological parts are often host strain sensitive. It is therefore necessary to develop genetic parts that are functional regardless of host strains.

A novel cell autolysis system for cost-competitive downstream processing.

The industrial production of low value-added biological products poses significant challenges due to cost pressures. In recent years, it has been argued that synthetic biology approaches will lead to breakthroughs that eliminate price bottlenecks for the production of a wide range of biological products including bioplastics and biofuels. One significant bottleneck lies in the necessity to break the tough cell walls of microbes in order to release intracellular products.