Revealing the different effects induced by antipsychotic drugs using an electrochemical microsensor to measure exocytosis in living cells.

Real-time monitoring of neurotransmitter release in living cells is crucial for understanding neural functions and the efficacy of drug actions. Here, we developed an electrochemical microsensor chip using a multidimensional nanosensitive material (MNG-1) that detects dopamine (DA) with high sensitivity, enabling real-time analysis of exocytosis in living cells. Our sensor-based technique does not require advanced equipment and can detect single exocytotic events using a standard electrochemical workstation and a small Faraday cage, allowing for rapid and statistically significant data collection.

Ultrasound treatment improves the quality and nutritional stability of tender coconut water during storage: Mechanistic insights into enzymatic inhibition.

This study investigated the effects of ultrasound (US) treatment on tender coconut water (TCW), focusing on its quality, nutritional properties, antioxidant capacity, and enzymatic activity during storage. The inhibitory mechanisms of US on enzymatic reactions were further explored through structural characterization and molecular docking analysis. The results showed that US-treated TCW exhibited improved physicochemical stability, higher polyphenol content, and enhanced antioxidant activity during storage at 25 °C for 15 days.

Risk Factors for Cage Subsidence After Transforaminal Lumbar Interbody Fusion: A Systematic Review and Meta-Analysis.

Study Design: A systematic review and meta-analysis.

Background Summary: Cage subsidence (CS) is a common complication following transforaminal lumbar interbody fusion (TLIF) surgery.

Objective: This study aimed to identify and analyze risk factors associated with CS following TLIF.

Dynamic comparative analysis of moisture and flavour characteristics in green peppers (Piper nigrum L.) during thermal and non-thermal drying processes.

Dynamic changes in moisture and volatile organic compounds (VOCs) of green pepper (GP) during hot-air drying (HAD), far-infrared drying (FIRD), and freeze drying (FD) were investigated. Results showed HAD increased terpenoids peak area by 44.72 %, the drying time of FIRD was only 5 h, and the FD piperine retention was 135 %.

Combined bactericidal effect of linalool and pulsed light on Vibrio parahaemolyticus and its application to black tiger prawns.

Vibrio parahaemolyticus (V. parahaemolyticus) is a foodborne pathogen from the sea that has a significant negative impact on the dietary safety and quality of aquatic products. In this study, the effects and mechanisms of the combined sterilization of V.

. Polyphenol Extract Alleviates NAFLD via Regulating Gut Microbiota and AMPK/AKT/mTOR Signaling Pathway.

With the drastic changes in lifestyle, nonalcoholic fatty liver disease (NAFLD) has become a widespread health problem. Natural actives such as polyphenols have multi-target, multi-mechanism characteristics, and offer new opportunities for NAFLD treatment. This study established a high-fat diet (HFD)-induced NAFLD model in mice to investigate the molecular mechanism of polyphenol extract (PAE) in alleviating NAFLD.

Improving the Sensory Quality of Black Tea by Blending Varieties During Processing.

Tea blending technology is based on finished tea. Blending fresh leaves during processing has not been proposed and investigated anywhere. This study investigates the impact of blending fresh leaves from different varieties on the flavor quality of black tea.

Impact of ultrasound on the quality of tender coconut water during storage revealed by LC-MS-based metabolomics, HS-SPME-GC-MS and E-tongue.

Tender coconut water (TCW) refers to the liquid endosperm of coconut developed in 6-9 months, which is prone to deterioration during postharvest storage. This study explored the impact of ultrasound (20 kHz, 2400 w and 20 min) on the quality of TCW during postharvest storage. Results showed that ultrasound was effective in preserving the quality of TCW.

Comprehensive evaluation of the nutritional value and contaminants of alfalfa ( L.) in China.

Introduction: Alfalfa is used as a feed source for animals and plays an important role in animal nutrition. Nutritional value analysis and contamination evaluation are essential for sustainable utilisation to ensure the quality and safety of alfalfa.

Aim: We aimed to evaluate the nutritional value and safety of alfalfa in five major regions in China-the Northeast Plain (NP), Inner Mongolia Plateau (IMP), Northwest Desert Oasis (NO), Loess Plateau (LP), and Huanghuaihai Plain (HP).

Maillard reaction inducing amino acids degradation can adjust the flavour characteristic of black tea.

Drying is the step that is to be used to adjust and control the formation of flavour and quality in black tea processing. In the present work, the comprehensive two-dimensional gas chromatography with mass spectrometry (GC × GC-MS) and gas chromatography olfactometry with mass (GC-O-MS) were used to determine the dynamic change of the volatile compounds in black tea during drying at 90, 120, 150 °C for 1 h. Results showed that the ratio of esters and aldehydes largely declined when temperature was elevated from 90 °C to 150 °C, while the ratio of heterocycles was increased to 22.

Evaluation of key taste components in Huangjin green tea based on electronic tongue technology.

A random forest algorithm combined with correlation analysis, was employed to identify key taste compounds in Huangjin green tea by using an electronic tongue. A total of 45 commercial Huangjin green teas were analysed for their amino acids, catechins, gallic acid, and caffeine using an amino acid analyser and HPLC. In this study, taste compounds of 30 were quantified, and 16 of these compounds exhibited taste activity values greater than 1 in the tea samples, including 6 amino acids, 8 catechins, as well as gallic acid and caffeine.

Non-targeted metabonomics reveals the effect of linalyl alcohol on Brochothrix thermophile and its potential application.

Brochothrix thermophcta (B. thermophcta) is a pathogenic microorganism associated with food contamination. Linalyl alcohol, owing to its broad spectrum and exceptional antibacterial properties, is regarded as a potent natural antimicrobial agent.

Structural and physicochemical properties of pea starch dual-treated with dry heating and galactomannans.

The research on the combination of starch and galactomannans (GM) with dry heat treatment (DHT) is currently insufficient, which hinders the starch application. In this study, the impacts of dry heat treatment and GM complex on the structural, gelatinization properties, and digestibility of pea starch (PS) were investigated. The gelatinization viscosity and gel hardness of dry heated-PS were decreased.

Safety and efficacy of intracardiac echocardiography-guided zero-fluoroscopy ablation in atrial fibrillation patients: a comparative study of high-power short-duration and low-power long-duration strategies.

Introduction: In atrial fibrillation (AF) ablation, fluoroscopy has been a standard tool for catheter guidance. However, the combination of electroanatomic mapping systems (EAMs) and intracardiac echocardiography (ICE) now allows for minimal or zero-fluoroscopy procedures. Concurrently, high-power short-duration (HPSD) ablation has emerged as a promising technique, offering enhanced resistive heating while reducing conductive heating.

Membrane Damage and Metabolic Disruption as the Mechanisms of Linalool against : An Amino Acid Metabolomics Study.

() is usually detected in low-temperature meat products, and seriously threatens food safety and human health. Therefore, the study investigated the antibacterial mechanism of linalool against from membrane damage and metabolic disruption. Results from field-emission transmission electron microscopy (FETEM) and atomic force microscopy (AFM) showed that linalool damage membrane integrity increases surface shrinkage and roughness.

Polysaccharides from Alpinia oxyphylla fruit prevent hyperuricemia by inhibiting uric acid synthesis, modulating intestinal flora and reducing renal inflammation.

Hyperuricemia (HUA) is one of the most common chronic diseases today, with a prevalence exceeding 14 % in both the United States and China. Current clinical treatments for HUA focus on promoting uric acid (UA) excretion and inhibiting UA production, but often neglect the strain on the liver and kidneys. The fruit of Alpinia oxyphylla (A.

Disruption of Cell Membranes and Redox Homeostasis as an Antibacterial Mechanism of Dielectric Barrier Discharge Plasma against .

Direct barrier discharge (DBD) plasma is a potential antibacterial strategy for controlling () in the food industry. The aim of this study was to investigate the inhibitory effect and mechanism of action of DBD plasma on . The result of the antibacterial effect curve shows that DBD plasma has a good inactivation effect on .

Ultralong luminescence lifetime imaging of edible plant tissue for humidity sensing in food packaging by a smartphone.

The safety of luminescence sensors and probes used in food packaging should be seriously considered, while most luminescence sensors were artificially synthesized with unclear toxicity, and cannot be directly used as indicators that were in contact with food. To overcome this problem, a humidity indicator based on an edible plant tissue was developed without any chemical processing. We found that garlic bulbs could emit significant persistent luminescence after drying at room temperature.

Constructing artificial gap junctions to mediate intercellular signal and mass transport.

Natural gap junctions are a type of channel protein responsible for intercellular signalling and mass communication. However, the scope of applications for these proteins is limited as they cannot be prepared at a large scale and are unable to spontaneously insert into cell membranes in vitro. The construction of artificial gap junctions may provide an alternative strategy for preparing analogues of the natural proteins and bottom-up building blocks necessary for the synthesis of artificial cells.

Mechanism for improving the in vitro digestive properties of coconut milk by modifying the structure and properties of coconut proteins with monosodium glutamate.

In this paper, the effect of monosodium glutamate (MSG) on coconut protein (CP) solubility, surface hydrophobicity, emulsification activity, ultraviolet spectroscopy and fluorescence spectroscopy was investigated. Meanwhile, the changes in the in vitro digestive properties of coconut milk were also further analyzed. MSG treatment altered the solubility and surface hydrophobicity of CP, thereby improving protein digestibility.

Photocatalytic inactivation mechanism of nano-BiPO against Vibrio parahaemolyticus and its application in abalone.

Vibrio parahaemolyticus (V. parahaemolyticus) is the main pathogenic bacteria in seafood that can cause serious food-borne illness. The annual incidence of V.

Effects of protein concentration, ionic strength, and heat treatment on the interfacial and emulsifying properties of coconut ( L.) globulins.

This research aimed to investigate the effects of protein concentration (0.2 %-1.0 %), ionic strength (100-500 mM NaCl), and heat treatment (temperature: 80 and 90℃; time: 15 and 30 min) on the interfacial and emulsifying properties of coconut globulins (CG).

Effect of steam explosion on the morphological, textural, and compositional characteristics of betel nut.

To reduce the adverse physical effects on the oral mucosa caused by excessive hardness of betel nut fibers, steam explosion was used to soften betel nuts. The effect of three operating parameters (pressure holding time, explosion pressure, and initial moisture content) on the morphology, texture, and chemical composition of the betel nuts was investigated. The fiber hardness and Shore hardness decreased by 56.