Specific Pathogen Free Ten Gene-Edited Donor Pigs for Xenotransplantation.

Xenotransplantation has entered the clinical phase in an effort to address the global organ shortage. However, recent clinical studies have revealed that current xenografts from gene-edited (GE) pigs still pose risk of immune rejection and biosafety concerns. In this study, we successfully produced a large batch of 582 GE cloned donor (GEC) pigs with 10-(GTKO/CMAHKO/β4GalNT2KO/hCD46/hCD55/ hCD59/hTBM/ hEPCR/hCD39/hCD47) gene edits via gene editing and somatic cell cloning technologies, and successfully obtained the F1 generation.

Histological Evaluation of Mandibular Bone After Mandibular Outer Cortex Osteotomy: The Role of the Periosteum.

Objective: This study investigates the role of the periosteum in the repair and reconstruction process post-osteotomy using histological analysis to provide insights for pediatric craniofacial surgery.

Methods: Twenty-four 3-month-old miniature pigs were divided into 2 groups. Group I: left mandibular outer cortex and periosteum resection + right mandibular outer cortex resection with periosteum preserved.

MicroRNA analysis of porcine muscle tissue involved in phosphoinositol metabolism.

Introduction: To elucidate the role of miRNAs in porcine muscle tissues, this study used Solexa high-throughput sequencing to identify and compare differentially expressed miRNAs in Landrace pigs and the Diannan small ear pigs.

Methods: Small RNA libraries were constructed from high-quality RNAs extracted from the Longissimus dorsi muscle (LDM) muscles of the two breeds of pigs and high-throughput sequencing was performed to identify and compare differentially expressed of miRNAs (DEmiRNAs). The target genes corresponding to the differentially expressed miRNAs were subjected to functional annotation, Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genomes (KEGG) pathway enrichment analysis.

The role of adenoid hypertrophy in obstructive sleep apnea outcomes post-mandibular distraction osteogenesis in patients with craniofacial microsomia.

Study Objectives: Mandibular distraction osteogenesis (MDO) is recommended for children with craniofacial microsomia (CFM) and obstructive sleep apnea (OSA). However, its efficacy remains limited, with success rates of 36.4%-60%.

A Comparative Study of ChatGPT-4o and DeepSeek Responses to Mandibular Angle Osteotomy Questions.

Background: Mandibular angle osteotomy (MAO) is one of the most effective ways to correct square facial contours. With the development of Artificial Intelligence (AI) technology, particularly in medicine, more patients are seeking medical queries from online websites. This study compared the performance of 2 AI platforms, ChatGPT-4o and DeepSeek in answering questions about MAO.

PIK-III-Mediated Elevation of Thiamine Re-Sensitises Renal Cell Carcinoma to Cuproptosis via Activating PDHA1.

Cuproptosis, a copper-dependent cell death mechanism driven by tricarboxylic acid (TCA) cycle collapse, shows limited efficacy in hypoxic or glycolytic renal cell carcinoma (RCC). Here, through systematic screening of 688 glycolysis inhibitors combined with elesclomol (ES), we identified PIK-III as a potent cuproptosis sensitiser. Multi-omics analysis revealed that PIK-III restores sensitivity by rewiring thiamine metabolism.

Effects of Stems and Leaves of on mRNA Expression of TLR Signaling Pathway in Hepatic Tissue of Duzang Pigs.

: stems and leaves (PNSLs) have shown limited adoption as a forage component in commercial livestock operations due to low utilization rates. : This study was designed to add 10% and 20% PNSLs in the diet of Duzang pigs. Hepatic tissues were collected to investigate the expression levels of Toll-like receptor (), , genes, and downstream cytokines within the TLR signaling pathway.

Spongiotic Psoriasiform Dermatitis with Dual Features of Eczema and Psoriasis: JAK Inhibition as a Potential Therapeutic Option.

Background: Psoriasis and eczema are inflammatory skin disorders with distinct immune mechanisms. Psoriasis is mainly driven by Th17 immune responses, while eczema, notably atopic dermatitis (AD), is Th2-mediated. Spongiotic psoriasiform dermatitis (SD/PSO), a hybrid phenotype of both, lacks precise immunophenotypic characterization and treatment strategies.

The Role of Heparan Sulfate in Bone Repair and Regeneration.

Heparan sulfate (HS) is synthesized in majority of cells in the human body and is typically released as an extension of polysaccharide chains, which then covalently bind to the protein core of proteoglycans to form heparan sulfate proteoglycans (HSPG). Research has shown that HS plays a crucial role in various cellular pathways of bone repair and regeneration and this review aims to summarize the role of HS in the above process. We found that the specific role of HS in bone repair and regeneration may depend on its location by analyzing the published research.

Bile acids affect intestinal barrier function through FXR and TGR5.

Bile acids play a dual role by aiding lipid absorption and acting as signaling molecules by interacting with various receptors. Bile acids are perpetually recycled via enterohepatic circulation and are biotransformation by gut microbiota, making bile acid metabolism a critical regulator of intestinal homeostasis. The intestinal epithelium prominently expresses two key bile acid receptors - the farnesoid X receptor (FXR) and G protein-coupled bile acid receptor 1 (TGR5) - which play indispensable roles in maintaining bile acid homeostasis and intestinal barrier function.

Fine-grained image generation with EEG multi-level semantics.

Background And Objective: Decoding visual information from electroencephalography (EEG) signals is crucial in neuroscience and artificial intelligence. While existing methods have been able to extract high-level features such as object categories, the capability of extracting fine-grained attributes, such as color distribution, remains insufficient. In this work, we propose EEG2IM, a novel framework that integrates multi-level EEG semantic features to guide a diffusion model for fine-grained image generation.

Investigation of Infection of and in Beef Cattle in Yunnan, China.

and are major zoonotic pathogens that often cause diarrhea in immunocompromised humans or animals. Beef cattle are important reservoirs for these two pathogens. Yunnan Province is a major region for beef cattle farming, and its suitable climatic conditions facilitate the transmission of the pathogens.

First successful treatment of epidermolytic Ichthyosis with Vunakizumab: A Case Report.

Ichthyoses, a group of skin cornification disorders caused by protein and lipid abnormalities that disrupt epidermal functions, are mainly characterized by generalized scaling. This study is the first to report the use of Vunakizumab, China's first self-developed anti-IL-17A monoclonal antibody, in treating ichthyosis. We presented a case of a 4-year-old boy with epidermolytic ichthyosis (EI) due to a gene mutation.

MT-RCAF: A Multi-Task Residual Cross Attention Framework for EEG-based emotion recognition and mood disorder detection.

Background And Objective: Prolonged abnormal emotions can gradually evolve into mood disorders such as anxiety and depression, making it critical to study the relationship between emotions and mood disorders to explore the causes of mood disorders. Existing research on EEG-based emotion recognition and mood disorder detection typically treats these two tasks separately, missing potential synergies between them. The purpose is to reveal the relationship between emotions and mood disorders and propose a Multi-Task Residual Cross Attention Framework (MT-RCAF) to enhance both classification performances.

Comparative Effectiveness of Infliximab vs Ustekinumab for Endoscopic and Transmural Remission in Biologic Naïve Crohn's Disease.

Background & Aims: Active-comparator studies are important to clinical decision-making. We compared the effectiveness of infliximab vs ustekinumab for endoscopic and transmural remission in biologic-naïve Crohn's disease (CD).

Methods: This was a prospective real-world cohort study that included biologic-naïve patients with CD initiating infliximab or ustekinumab therapy.

Association Between Dietary Soy Isoflavones Intake and the Risk of Hyperemesis Gravidarum: A Cross-Sectional Study in Chinese Pregnant Women.

(1) Background: Diet plays a crucial role in the intake of phytoestrogens, which are closely related to the pathogenesis of some pregnancy complications. However, no studies have explored the potential association between soy isoflavones, a type of phytoestrogen, and the risk of hyperemesis gravidarum (HG). This study aims to investigate the correlation between dietary intake of soy isoflavones and the risk of HG.

Comparative Analysis of Short-Chain Fatty Acids and the Immune Barrier in Cecum of Dahe Pigs and Dahe Black Pigs.

The intestinal immune barrier is a developed and complex immune system, and there is a fine synergy between it and the induced immune response. Short-chain fatty acids (SCFAs) are the main metabolites of intestinal microbial fermentation. In the cecum of pigs, SCFAs not only provide energy for the host but also participate in regulating the function of the intestinal immune system.

The stems and leaves of reduce the abundance of antibiotic resistance genes by regulating intestinal microbiota in Duzang pigs.

In order to study the distribution characteristics of intestinal microbiota and antibiotic resistance genes (ARGs) in Duzang pigs after adding stems and leaves of to the feed, the characteristics of intestinal microbiota were explored by metagenomic sequencing, and 14 ARGs and 2 integrase genes were detected by qPCR. The results showed that the addition of stems and leaves of increased the relative abundance of Firmicutes, and in the cecum of Duzang pigs. A total of 10 ARGs and 2 integrase genes were detected in the cecal contents of pigs.

Mechanism of PAVA-induced toxicity and inflammation in a cocultured skin cell model.

Background: Pelargonic acid vanillyl amide (PAVA), a stable synthetic analog of capsaicin, exhibits potential for therapeutic applications; however, it may present cytotoxic and pro-inflammatory risks. This study aims to investigate the injury effects of PAVA on a cocultured skin cell model .

Methods: Human keratinocytes and dermal fibroblasts were co-cultured and exposed to PAVA at concentrations ranging from 12.

Melanin deposition and key molecular features in Xenopus tropicalis oocytes.

Background: Melanin pigmentation in oocytes is a critical feature for both the esthetic and developmental aspects of oocytes, influencing their polarity and overall development. Despite substantial knowledge of melanogenesis in melanocytes and retinal pigment epithelium cells, the molecular mechanisms underlying oocyte melanogenesis remain largely unknown.

Results: Here, we compare the oocytes of wild-type, tyr and mitf Xenopus tropicalis and found that mitf oocytes exhibit normal melanin deposition at the animal pole, whereas tyr oocytes show no melanin deposition at this site.

Association of Antioxidant Diet with Risk of Hyperemesis Gravidarum Among Chinese Pregnant Women: A Population-Based Cross-Sectional Study.

(1) Background: Oxidative stress plays a pivotal role in the pathophysiologic of hyperemesis gravidarum (HG). Epidemiological studies have explored the associations of specific antioxidant foods and nutrients with HG. However, evidence regarding the relationship between an antioxidant-rich diet and the risk of HG remains limited.

The Role of Pyroptosis in the Progression and Targeted Therapeutic Approaches for Urological Malignancies.

The prevalence of urological malignancies continues to pose a significant global health challenge, particularly due to the poor prognosis associated with advanced stages of these diseases. Consequently, there is an urgent need to deepen our understanding of the molecular mechanisms governing the development of urological malignancies to facilitate breakthroughs in diagnosis and treatment. Pyroptosis, a novel and specific form of programmed cell death, plays a crucial role in regulating inflammatory responses, cell development, tissue homeostasis, and stress responses.

A research on improving the precision of laser tracker in alignment based on Pareto improvement principle.

The accuracy of laser trackers in large-scale dimensional metrology is subject to various influencing factors, with instrument positioning being a primary source of error. To address this, a multi-objective optimization algorithm, grounded in the Pareto improvement principle, is proposed. This algorithm is designed to optimize instrument placement, thereby minimizing measurement errors and enhancing the algorithm's efficacy in error reduction.

Targeted metabolomics of muscle amino acid profles and hepatic transcriptomics analyses in grass carp () fed with broad beans.

While tissue amino acid compositions reflect that of the dietary protein source, and the liver orchestrates amino acid metabolism. In this study, we investigated the muscle amino acid profiles in ordinary and crisp grass carp. The 22 amino acids were measured, and seventeen showed significant concentration differences.