Specific Pathogen Free Ten Gene-Edited Donor Pigs for Xenotransplantation.

Xenotransplantation has entered the clinical phase in an effort to address the global organ shortage. However, recent clinical studies have revealed that current xenografts from gene-edited (GE) pigs still pose risk of immune rejection and biosafety concerns. In this study, we successfully produced a large batch of 582 GE cloned donor (GEC) pigs with 10-(GTKO/CMAHKO/β4GalNT2KO/hCD46/hCD55/ hCD59/hTBM/ hEPCR/hCD39/hCD47) gene edits via gene editing and somatic cell cloning technologies, and successfully obtained the F1 generation.