Single-Cell RNA Sequencing Reveals Potential Mechanism of RUNX3 Reshaping Tumor Microenvironment in Non-small-cell Lung Cancer.

Background: RUNX3 acts as a tumor suppressor gene in non-small-cell lung cancer (NSCLC), yet its specific biological mechanism is still unclear. This study aimed to uncover tumor microenvironment (TME) changes in NSCLC with varying RUNX3 expression statuses through single-cell RNA sequencing.

Patients And Methods: In total, seven patients with NSCLC with detailed pathological data were involved, with three both paracancerous and cancerous tissue samples.

Correction: Dual roles of proteins in macrophage immune dynamics.

[This corrects the article DOI: 10.3389/fimmu.2025.

Dual roles of proteins in macrophage immune dynamics.

Background: Anisakis infections have become a significant public health concern primarily caused by consuming raw or undercooked seafood. This in is due to a shift in global eating habits, and seafood consumption is becoming increasingly popular.

Materials And Methods: This study explores how body proteins (ABP) and glycoproteins affect macrophage polarization.

Transcriptome-based analysis of the immune response of the four-finger threadfin (Eleutheronema tetradactylum) to Vibrio harveyi infection.

The four-finger threadfin (Eleutheronema tetradactylum), an economically important fish species, has faced significant economic losses due to bacterial diseases, particularly Vibrio harveyi, a Gram-negative bacterium. In this study, we used RNA sequencing (RNA-seq) to analyze transcriptomic responses in the intestine and liver of E. tetradactylum post-V.

Aptamer as a Molecular Tethering Agent Induces PrP Aggregation and Degradation to Inhibit Melanoma Proliferation.

Melanoma, a malignant tumor originating from melanocytes, is the most aggressive and deadly form of skin cancer. Previous studies have revealed that the cellular prion protein (PrP) is frequently overexpressed in melanoma, contributing to tumor progression. This study presents the first proof of concept evidence that nucleic acid aptamers can be used to construct a molecular tethering agent that regulates PrP protein levels by inducing membrane-bound PrP aggregation for antimelanoma therapy.

Current Status and Factors Influencing Nutrition Literacy in Stroke Patients: A Cross-Sectional Study.

Aims: This study investigated the current status of nutrition literacy and related influencing factors in stroke patients, with a view to providing a reference for the development of targeted interventions.

Design: Cross-sectional study.

Methods: A convenience sampling method was used to select 342 stroke patients from June to November 2024 as the study population, and a cross-sectional survey was conducted using the General Information Questionnaire, Nutrition Literacy Scale, Herth Hope Scale, Chronic Disease Self-Efficacy Scale and Social Support Rating Scale.

Origin, Evolution, and Diversification of the Expansin Family in Plants.

The cell wall is a crucial feature that allows ancestral streptophyte green algae to colonize land. Expansin, an extracellular protein that mediates cell wall loosening in a pH-dependent manner, could be a powerful tool for studying cell wall evolution. However, the evolutionary trajectory of the expansin family remains largely unknown.

Combined Use of Magnetization Transfer Ratio and T2-Mapping to Evaluate Extraocular Muscle Pathophysiology in Myasthenia Gravis with Ophthalmoparesis.

Myasthenia gravis (MG) is an autoimmune neuromuscular disorder that most frequently affects the extraocular muscles (EOMs), which causes symptoms such as ptosis and restricted eye movement. The EOMs in MG patients are representative of autoimmune inflammatory changes in muscle tissue. Currently, there is no reliable, and sensitive imaging technique for monitoring EOM changes to assist in the evaluation of underlying pathological changes.

Circulating CD45RAFoxp3 Treg cells serve as a biomarker for predicting minimal clinical manifestations status of myasthenia gravis.

Aims: Regulatory T cells (Tregs) are key mediators of the induction of immune tolerance; however, the mechanisms by which they regulate myasthenia gravis (MG) are not fully understood. This study aimed to explore the characteristics of Tregs and their subpopulations in the peripheral blood of patients with minimal clinical manifestations (MM) of MG and identify biomarkers that predict MM-MG for treatment guidance.

Materials And Methods: The clinical data of patients with general MG who visited our hospital were retrospectively analyzed.

Conventional dendritic cells are more activated in the hyperplastic Thymus of myasthenia gravis patients.

Introduction: Dendritic cells (DCs) are crucial to form ectopic germinal centers (GCs) in the hyperplastic thymus (HT), which are typically found in anti-acetylcholine receptor autoantibody-positive myasthenia gravis (MG) patients. However, the characteristics of such DCs in the HT and their roles in thymic hyperplasia formation remain unclear.

Methods: We collected thymic tissue from MG patients and patients who underwent cardiac surgery.

DesTrans: A medical image fusion method based on Transformer and improved DenseNet.

Medical image fusion can provide doctors with more detailed data and thus improve the accuracy of disease diagnosis. In recent years, deep learning has been widely used in the field of medical image fusion. The traditional method of medical image fusion is to operate by superimposing and other methods of pixels.

gene is essential for the larval growth and development of silkworm, .

The genetic male sterile line () of the silkworm is a recessive mutant that is naturally mutated from the wild-type strain. One of the major characteristics of the mutant is its small larvae. Through positional cloning, candidate genes for the mutant were located in a region approximately 800.

Genetic and transcriptome analysis of the smb mutant in Bombyx mori.

More than 600 mutations have been discovered in the history of silkworm domestication. It is important to study the formation mechanism of these mutations to further understand the life and development process of silkworms and agricultural pest control. The silkworm mutant smb was isolated from silkworm strain NCV, and transcriptome analysis was performed on the silkworm mutant.

Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.

Background: Cells and tissues in an inflammatory state are usually hypoxic. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α). Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Predictive Value of Post-Percutaneous Coronary Intervention Quantitative Flow Ratio for Vessel-Oriented Composite Endpoint.

At present, there is a lack of indicators, which can accurately predict the post-percutaneous coronary intervention (post-PCI) vessel-oriented composite endpoint (VOCE). Recent studies showed that the post-PCI quantitative flow ratio (QFR) can predict post-PCI VOCE. PubMed, Embase, and Cochrane were searched from inception to March 27, 2022, and the cohort studies about that the post-PCI QFR predicts post-PCI VOCE were screened.

Long-term efficacy and safety of leflunomide combined with low-dose prednisone in treatment of myasthenia gravis: a retrospective study.

Background: Leflunomide and low-dose prednisone (0.25 mg/kg/day) (LEF + Pred) rapidly improved the clinical symptoms of myasthenia gravis (MG) patients. Here, we aimed to analyze the long-term efficacy and safety of LEF + Pred in MG patients.

Single-cell spatial transcriptome reveals cell-type organization in the macaque cortex.

Elucidating the cellular organization of the cerebral cortex is critical for understanding brain structure and function. Using large-scale single-nucleus RNA sequencing and spatial transcriptomic analysis of 143 macaque cortical regions, we obtained a comprehensive atlas of 264 transcriptome-defined cortical cell types and mapped their spatial distribution across the entire cortex. We characterized the cortical layer and region preferences of glutamatergic, GABAergic, and non-neuronal cell types, as well as regional differences in cell-type composition and neighborhood complexity.

Corrigendum: Leflunomide combined with low-dose prednisone inhibits proinflammatory T cells responses in myasthenia gravis patients.

[This corrects the article DOI: 10.3389/fneur.2022.

Alcoholic cardiomyopathy in patients with alcoholic liver cirrhosis: a study across 10 years.

Background And Objectives: Available data regarding cardiomyopathy in patients with alcoholic liver cirrhosis (ALC) are very limited because it often requires multidisciplinary assessments. The study aims to evaluate the prevalence of alcoholic cardiomyopathy in ALC and their clinical correlations.

Methods: Adult ALC patients without a previous diagnosis of cardiovascular diseases between January 2010 and December 2019 were included in the study.