Hypoxia Triggers ALYREF-Mediated mC Methylation of KIF20A to Activate KIF20A/BUB1 for Generating Ferroptosis Resistance in Cervical Cancer Cells.

Ferroptosis resistance is a key player in cervical cancer (CC) development. Hypoxia is a negative factor affecting CC treatment by inducing ferroptosis resistance. Our study aimed to investigate the detailed mechanisms of hypoxia-induced ferroptosis resistance in CC cells.

Towards Biosignals-Free Autonomous Prosthetic Hand Control via Imitation Learning.

Limb loss affects millions globally, impairing physical function and reducing quality of life. Most traditional surface electromyographic (sEMG) and semi-autonomous methods require users to generate myoelectric signals for each control, imposing physically and mentally taxing demands. This study aims to develop a fully autonomous control system that enables a prosthetic hand to automatically grasp and release objects of various shapes using only a camera attached to the wrist.

Uniform Cu-Based Metal-Organic Framework Micrometer Cubes with Synergistically Enhanced Photodynamic/Photothermal Properties for Rapid Eradication of Multidrug-Resistant Bacteria.

: The rapid emergence of multidrug-resistant bacterial infections demands innovative non-antibiotic therapeutic strategies. Dual-modal photoresponse therapy integrating photodynamic (PDT) and photothermal (PTT) effects offers a promising rapid antibacterial approach, yet designing single-material systems with synergistic enhancement remains challenging. This study aims to develop uniform Cu-based metal-organic framework micrometer cubes (Cu-BN) for efficient PDT/PTT synergy.

In vivo detection of macrophage-like cells at the vitreoretinal interface in retinal diseases using optical coherence tomography angiography.

Retinal diseases represent a group of vision-threatening disorders with a complex pathogenesis that involves interactions among various cellular and molecular factors. Inflammation and immune cells play a pivotal role in the initiation, progression, and repair of retinal diseases. Recently, advancements in optical coherence tomography angiography (OCTA) technology have enabled the in vivo detection of macrophage-like cells (MLCs) at the vitreoretinal interface and allowed for assessment of retinal inflammation, representing a landmark advancement for understanding the pathophysiology of retinal diseases, particularly regarding the role of MLCs.

Effects of COVID-19 vaccination on irAEs and prognosis in lung cancer patients receive PD-(L)1 inhibitors.

The influence of COVID-19 vaccination on the efficacy and safety of immune checkpoint inhibitors (ICIs) in lung cancer treatment is not well understood. A cohort of 394 lung cancer patients treated with PD-1/PD-L1 inhibitors was analyzed. The incidence and types of irAEs were recorded, and the relationship between COVID-19 vaccination and progression-free survival (PFS) was assessed using univariate and multivariate analyses.

Machine learning and network toxicology reveal arsenic as a key driver of non-carcinogenic health risks from heavy metal residues in Chinese medicinal plants.

Heavy metal (HM) residues in Chinese medicinal plants (CMPs) threaten their quality and human health. This study investigated anthropogenic drivers of HMs accumulation, evaluated key HMs contributing to the non-carcinogenic hazard index (HI), and explored health risks. Arsenic (As), lead (Pb), mercury (Hg), cadmium (Cd), and copper (Cu) residues correlated with energy consumption, industrial activities, and agricultural practices.

Function and Regulatory Mechanism of a Two-Component Signal Transduction System cgtRS5 in Corynebacterium glutamicum.

The putative two-component system (TCS) CgtRS5, encoded by the ncgl2572-ncgl2573 gene cluster, plays a critical role in environmental adaptation in Corynebacterium glutamicum. Comparative transcriptomic analysis between the wild-type strain and a ΔcgtRS5 mutant revealed three upregulated and 107 downregulated genes in the mutant. Among these, genes involved in iron metabolism (ncgl1959), aromatic compound degradation (ncgl2320), and drug resistance (cssR) were significantly downregulated.

Robust and efficient MS@CTFs for simultaneous removal of enrofloxacin and cationic dye.

A novel adsorbent named as MS@CTFs, was successfully synthesized through the Schiff base reaction by immobilizing a covalent triazine framework (CTF) constructed from 2,4,6-tris(4-aminophenyl)-1,3,5-triazine (TAPT) and trimethylformyl resorcinol (TP) onto a melamine sponge (MS) substrate. The innovative design integrated robust triazine-based CTF with an easy-to-use melamine sponge, simplifying the sample preparation procedure and also enhancing the adsorption performance. After optimizing the adsorption parameters, the MS@CTFs demonstrated robust adsorption for enrofloxacin (ENR) from milk matrices, the adsorption capacity was 155.

Effect of active mind-body movement therapies on older osteoarthritis: a systematic review and meta-analysis of randomized controlled trials.

Objective: To systematically review the literature to quantify and compare the effects of active mind-body movement therapies (AMBMTs) on pain, stiffness, and joint function in older adults with osteoarthritis (OA).

Methods: We searched PubMed, Embase, Cochrane Register of Controlled Trials, Web of Science, ScienceDirect, CINAHL, and PEDro. The outcome measures included the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and the 36-item Short Form Health Survey (SF-36).

ATP1A1-Driven Intercellular Contact Between Dental Pulp Stem Cell and Endothelial Cell Enhances Vasculogenic Activity.

Aim: The interaction between dental pulp stem cells (DPSCs) and vascular endothelial cells (ECs) is crucial to the speedy establishment of functional blood circulation within the transplanted pulp tissue. It is a complex process involving direct cell contact and paracrine signalling. The transmembrane domains of α1-Na/K-ATPase (ATP1A1) have been shown to influence tumour angiogenesis.

Cathepsin H increases the risk of diabetic retinopathy: evidence from Mendelian randomization and bioinformatic analysis.

Background: While lysosomal cathepsins are crucial in cellular homeostasis and may contribute to diabetic chronic complications, their precise causal relationships remain insufficiently characterized.

Methods: This study employed comprehensive multidimensional Mendelian randomization (MR) analyses to investigate potential causal associations between nine cathepsins (B, E, F, G, H, L2, O, S, and Z) and six diabetic chronic complications encompassing both microvascular (nephropathy, retinopathy, proliferative retinopathy, maculopathy, neuropathy) and macrovascular manifestations (peripheral angiopathy). The analytical framework incorporated generalized summary-data-based MR (GSMR), univariable MR, multivariable MR, summary-data-based MR (SMR), and cis-expression quantitative trait locus (cis-eQTL) MR approaches, utilizing data derived from publicly available genome-wide association studies (GWAS).

Fabrication of ultra-stable boronic acid-functionalized quinoline-linked COF for selective adsorption of quercetin.

Background: Quercetin, a flavonoid with cis-diol moiety, is widely found in plants and exhibits significant pharmacological properties, including anti-inflammatory, antioxidant, and anti-tumor activities. Efficient adsorption and separation of quercetin from complex matrices are crucial for advancing health and nutrition research. However, the structural diversity of flavonoids poses challenges for their selective separation.

Medulla oblongata dominated synaptic density network degeneration in amyotrophic lateral sclerosis.

Background: Amyotrophic lateral sclerosis (ALS) is a brain network disorder closely associated with synaptic loss in the upper and lower motor neurons. However, the in vivo synaptic network changes and their progressive processes remain unclear. Here, we aim to investigate the synaptic density network connectivity and the likely sequences of synaptic loss in patients with ALS.

Biomimetic Delivery of Cisplatin for Efficient Immunotherapy of Gasdermin E-Positive Hepatocellular Carcinoma via a Pt Concentration-Dependent Minimalist Pyroptosis Strategy.

Compared to the classical Gasdermin D (GSDMD)-mediated pyroptosis that has been extensively explored for cancer immunotherapy, the direct induction of Gasdermin E (GSDME) pyroptosis has been rarely reported, likely due to the well-recognized reduced GSDME levels in solid tumors. This study reports the use of a milestone chemotherapeutic drug, cisplatin (CDDP), for effectively inducing inflammatory pyroptosis that depends on the intracellular platinum (Pt) concentration in GSDME-positive hepatocellular carcinoma identified by bioinformatic analyses. Biomimetic delivery of CDDP via an internalizing arginine-glycine-aspartic acid (iRGD)-functionalized plasma exosome (iRGD-Pla-Exo) guarantees an effective tumor intracellular Pt concentration above the threshold for inducing prompt and potent pyroptosis with significantly compromised systematic side effects.

Efficacy and safety of Shuxuening injection in patients with unstable angina: a multicenter, randomized, placebo-controlled trial.

Objective: To determine the efficacy and safety of adjunct Shuxuening Injection (SXN) on conventional therapy in patients with unstable angina (UA) which provides a complementary choice and its evidence for clinical medication for treating UA.

Methods: In this multicenter, randomized, double-blind, placebo-controlled trial, 469 patients with UA from 25 hospitals in China who had an episode of angina within 48 hours were randomly assigned 3:1 to SXN and placebo group for 10 days in addition to conventional treatment. The primary outcome is the frequency of angina pectoris attacks after 10 days of intervention.

5-O-Methylvisammioside inhibits HMGB1-induced Angiogenesis of hepatocellular carcinoma through RAGE/MEK/ERK signaling pathway.

5-O-Methylvisammioside (5OMV), a flavonol compound derived from the traditional Chinese medicine plant Saposhnikovia divaricat, has been shown to inhibit vasospasm induced by High Mobility Group Box 1 (HMGB1) protein. However, its therapeutic potential and molecular mechanisms in HMGB1-induced tumor angiogenesis remain unexplored. Through comprehensive in vitro assays, we demonstrated that 5OMV significantly attenuates HMGB1-induced proliferation, migration, tube formation, and angiogenic activity in human umbilical vein endothelial cells (HUVECs).

Association between physical activity levels and mild cognitive impairment in Chinese older adults: a cross-sectional study from the China health and retirement longitudinal study.

Background: Research indicates that mild cognitive impairment (MCI) in older adults is linked to physical activity; however, the relationship between varying levels of physical activity (PAL) and the risk of MCI needs further exploration.

Objective: This study explores the association and dose-response relationship between different levels of physical activity and MCI in older adults.

Methods: Using data from the 2020 China Health and Retirement Longitudinal Study (CHARLS), this cross-sectional analysis included 5,373 older adults aged 60 and above.

OsMYB103 targets and interacts with AMD1 to form a feed-forward loop for pollen development in rice.

The process of male reproductive development in plants involves a series of biological events. These events are governed by complex transcriptional regulatory networks, which are composed of numerous transcriptional regulatory components (TRCs). ABERRANT MICROSPORE DEVELOPMENT1 (AMD1) is a recently identified TRC that is essential for rice pollen production.

The influence of SARS-CoV-2 spike protein exposure on retinal development in the human retinal organoids.

Background: Pregnant women are considered a high-risk population for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, as the virus can infect the placenta and embryos. Recently, SARS-CoV-2 has been widely reported to cause retinal pathological changes and to infect the embryonic retina. The infection of host cells by SARS-CoV-2 is primarily mediated through spike (S) protein, which also plays a crucial role in the pathogenesis of SARS-CoV-2.

A Metformin-Based Multifunctional Nanoplatform as a DNA Damage Amplifier for Maximized Radio-Immunotherapy to Overcome Radiotherapy Resistance.

Radiotherapy (RT) has been highlighted to be an effective strategy for antitumor immunity activation by causing direct DNA damages, but it generally suffers from low response rates due to the compromised cytosolic DNA (cDNA) recognition by cyclic GMP-AMP synthase (cGAS). Simultaneous DNA repair and clearance system regulation for enhanced cDNA accumulation is a useful approach to improve immune response rates, which remains seldom reported to our knowledge. Here, we report the construction of a metformin (MET)-based multifunctional nanocomplex, CS-MET/siTREX1 (CSMT), consisting of biguanide-decorated CS (CS-MET) as the vector and 3'-5' DNA exonuclease TREX1 siRNA (siTREX1) as the therapeutic gene for RT-induced antitumor immunity enhancement by amplifying the initial DNA damage signals.

Functional cobalt-doped hydrogel scaffold enhances concurrent vascularization and neurogenesis.

Achieving functional tissue regeneration hinges on the coordinated growth of intricate blood vessels and nerves within the defect area. However, current strategies do not offer a reliable and effective way to fulfill this critical need. To address this challenge, a three-dimensional (3D) gelatin methacryloyl-multi-walled carbon nanotube/cobalt (GelMA-MWCNTs/Co) hydrogel with controlled release of cobalt (Co) ions was developed for hypoxia-mimicking and dual beneficial effects on promoting vasculogenesis and neurogenesis.

Visible Light-Promoted Radical Cascade Bicyclization to Access Partially Saturated Pyrrolo[2,3-b]Pyridines.

A visible light-promoted cascade [2+2+1] bicyclization reaction of N-cyanamide alkenes and activated alkyl bromides has been successfully established. This reaction exhibits mild conditions, metal-free characteristics, and excellent atom- and step-economy, along with environmental friendliness. It demonstrates broad substrate tolerance and scalability to gram-scale synthesis, underscoring its potential for practical applications.

Artificial intelligence models for periodontitis classification: A systematic review.

Objectives: The graded diagnosis of periodontitis has always been a difficulty for dentists. This systematic review aimed to investigate the performance of artificial intelligence (AI) models for periodontitis classification.

Data: This review includes original studies that explore the application of AI in periodontitis classification systems.

Implicit learning of unfamiliar tone sandhi patterns in lexical recognition.

Introduction: This study investigates whether unfamiliar tone sandhi patterns in Tianjin Mandarin can be implicitly learned through an artificial language learning experiment, and if the acquired knowledge is rule-based and generalizable.

Methods: Participants were trained to learn monosyllabic words and disyllabic phrases with their attention focused on a word-order rule, while unknowingly being exposed to unfamiliar tone sandhi patterns. A judgement test with trial-to-trial confidence ratings was conducted to assess the learning outcomes and participants' awareness.