PCAT-1' s role in wound healing impairment: Mitochondrial dysfunction and bone marrow stem cell differentiation inhibition via PKM2/β-catenin pathway and its impact on implant osseo-integration.

This study focused on unravelling the role of PCAT-1 in wound-healing process, particularly its impact on regenerative and osteogenic abilities of mesenchymal stem cells (MSCs). We delved into how PCAT-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) and interacts with pivotal molecular pathways, especially β-catenin and PKM2, using human bone marrow-derived MSCs. MSCs were cultured under specific conditions and PCAT-1 expression was modified through transfection.

Treatment of Advanced Gingival Recession Secondary to Surgical Failure with Large Size Deepithelized Gingival Graft Associated with a Modified Tunnel Flap.

Objective: To describe the use of a large size deepithelized gingival graft (DGG) associated with full-split tunnel technique in a clinical case of advanced gingival recession secondary to surgical failure (GRSF). . The presented case report helped to achieve satisfactory root coverage, ideal keratinized tissue gain, improvement in soft tissue quality and esthetics, scar deformity correction, and vestibular depth deepening with a one-step procedure of large size DGG associated with full-split tunnel technique for a condition of deep gingival recessions of 7-11 mm caused by a failed bone implantation surgery.

Fracture behaviors of maxillary central incisors with flared root canals restored with CAD/CAM integrated glass fiber post-and-core.

The objective was to evaluate the fracture resistance properties of maxillary incisors with flared canals restored with computer aided design and computer aided manufacture (CAD/CAM) integrated glass fiber post-and-core. Thirty prepared flared root canals were selected in vitro and restored with CAD/CAM integrated fiber post-and-core (Group A), prefabricated fiber posts (Group B), and cast gold alloy (Group C), respectively. After submitted to fatigue loading, each specimen was subjected to a static loading until fracture.

MiR-143 and miR-135 inhibitors treatment induces skeletal myogenic differentiation of human adult dental pulp stem cells.

Objective: Dental pulp stem cells (DPSCs) possess pluripotent properties that allow them to differentiate into multiple cell lineages, which can be potentially used in tissue regeneration. The aim of this in vitro study is to explore the effect of miRNAs on the myogenic differentiation of human adult DPSCs and seek for some potential biological factors for stable and feasible application in DPSC myogenic differentiation.

Methods: Human adult DPSCs were isolated from normal impacted third molars were treated with 5-Aza-2'-deoxycytidine to induce to myogenic differentiation in vitro.

A cross-sectional survey of dental caries, oral hygiene, and Helicobacter pylori infection in adults.

We explored the epidemiological risk factors for dental caries to help explain differences in the prevalence of adult dental caries. We examined 841 people for the presence of Helicobacter pylori in their dental plaque and for dental caries. Of the 841 subjects, 574 (68.

The protective roles of mitochondrial ATP-sensitive potassium channels during hypoxia-ischemia-reperfusion in brain.

The role of ATP-sensitive potassium (K(ATP)) channels in cerebral ischemia-reperfusion has been well documented. K(ATP) channel openers protect neuron by mimicking ischemic preconditioning. However, the different protection between the mitochondrial and sarcolemma K(ATP) openers has been seldom studied.

Association of three bacterial species and periodontal status in Chinese adults: an epidemiological approach.

Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia are oral pathogens associated with periodontitis. The association between these three bacteria and periodontal disease has been reported in populations of many countries. However, corresponding data in Chinese populations are still lacking.

Establishment of three-dimensional tissue-engineered bone constructs under microgravity-simulated conditions.

Bone constructs have been grown in vitro with use of isolated cells, biodegradable polymer scaffolds, and bioreactors. In our work, the relationships between the composition and mechanical properties of engineered bone constructs were studied by culturing bone marrow mesenchymal stem cells (BMSCs) on ceramic bovine bone scaffolds in different environments: static flasks and dynamic culture system in rotating vessels-which was a National Aeronautics and Space Administration-recommended, ground-based, microgravity-simulating system. After 15 days of cultivation, osteogenicity was determined according to DNA and alkaline phosphatase (ALP) analysis.

Multifunctional implantable particles for skin tissue regeneration: preparation, characterization, in vitro and in vivo studies.

The transplantation of cell-polymer constructs has been developed as a novel approach to curing tissue defects. However, a number of methodological problems remain to be solved, including the loss of a proper cellular milieu, the relatively long period of culture time and the complexity of the application. The aim of the present article is to evaluate the feasibility of porous gelatin-based implantable particles as a novel strategy for delivery of cultured cells and bioactive molecules to correct dermal defects.

Cartilage regeneration using a novel gelatin-chondroitin-hyaluronan hybrid scaffold containing bFGF-impregnated microspheres.

Cartilage engineered from chondrocytes requires a scaffold to keep the cells in the cartilage defect and to act as a support for inducing hyaline cartilage formation on occasion. In this study, we developed a novel three-dimensional special scaffold in combination with a controlled release of bFGF, which provided structural support and stimulated repair. Gelatin microspheres loaded with bFGF (GM-bFGF) showed a fast release at the initial phase (28.

Wound dressings containing bFGF-impregnated microspheres.

The primary objective was to synthesize a novel wound dressing containing basic fibroblast growth factor (bFGF)-loaded microspheres for promoting healing and tissue regeneration. Gelatin sponge was chosen as the underlying layer and elastomeric polyurethane membranes were used as the external layer. To achieve prolonged release, bFGF addition was loaded in microspheres.

[Promotion of wound healing through incorporation of bFGF-impregnated microspheres into collagen membrane].

Objective: To investigate the promotion effects of the collagen membrane incorporating bFGF-impregnated microspheres on the wound healing of the pig skin losing its full-thickness layers.

Methods: The bFGF containing microspheres was added into the dry microspheres. The collagen membranes were prepared by incorporating bFGF-impregnated microspheres, and 6 York pig models of skin wounds with loss of their full-thickness layers were established for the observation of the effects on the wound healing.