Ace2 mutation disrupts amino acid absorption, impairs growth, and alters microbiota dynamics in zebrafish.

Angiotensin-converting enzyme 2 (Ace2) is a well-studied enzyme with important physiological functions in mammals. However, its roles in non-mammalian animals remain largely unexplored. This study investigates the function of Ace2 in zebrafish using CRISPR/Cas9-generated ace2 mutants (-/-).

Bottom-Up Regulation of Perovskite Growth and Energetics via Oligoether Functionalized Self-Assembling Molecules for High-Performance Solar Cells.

Self-assembling molecules (SAMs) are widely used as interfacial layers to optimize the surface properties of nickel oxides (NiO) in inverted perovskite solar cells (PSCs). However, less attention is paid to the effect of SAMs on the regulation of perovskite growth and energetics. Here, based on the donor-acceptor molecular backbone, an oligoether chain is introduced with different chain lengths to endow two novel SAMs, namely, EPA and MEPA, with good capability of bottom-up regulation of perovskite formation and energetics.

Study on the mass transfer behavior of single CO bubbles in sodium silicate solution.

This study investigated in depth the mass transfer behavior of a single CO bubble in sodium silicate solution. The dynamics of the bubble were accurately tracked using high-speed camera technology, and key parameters such as velocity, drag coefficient and mass transfer coefficient during the bubble rising process were analyzed in detail with the data processing ability of MATLAB. The results showed that the concentration of sodium silicate solution had a significant impact on the dissolution behavior of the CO bubble.

Molecular Dynamics Analysis of the Solid-State Bonding Mechanism and High Strain Rate Response for (1 1 1)-Oriented Nanotwinned Silver.

This study employs molecular dynamics simulations to investigate the bonding mechanism and high strain rate response of (1 1 1)-oriented nanotwinned silver (NT-Ag). By constructing one idealized model with atomic-level flatness and one more realistic model considered surface roughness, it uncovers the atomic-level bonding process, revealing that the realistic model proposed more closely replicates real experimental characteristics, thereby demonstrating strong validity. High strain rate tensile tests show NT-Ag possessing a great impact resistance, with toughening mechanisms such as strain-induced amorphization.

The impact of admission modes on the treatment outcome and in-hospital mortality rate of STEMI patients undergoing PPCI.

The current research on ST elevation myocardial infarction (STEMI) patients has been mostly limited to Door-to-Balloon (D-to-B) time. This study aimed to compare the effects of different hospital admission modes to on the time metrics of patients undergoing primary percutaneous coronary intervention (PPCI). It also examined the effects of these modes on in-hospital mortality and other influencing factors.

Size Effects of Au/Ni-Coated Polymer Particles on the Electrical Performance of Anisotropic Conductive Adhesive Films under Flexible Mechanical Conditions.

In soft electronics, anisotropic conductive adhesive films (ACFs) are the trending interconnecting approach due to their substantial softness and superior bondability to flexible substrates. However, low bonding pressure (≤1 MPa) and fine-pitch interconnections of ACFs become challenging while being extended in advanced device developments such as wafer-level packaging and three-dimensional multi-layer integrated circuit board assembly. To overcome these difficulties, we studied two types of ACFs with distinct conductive filler sizes (ACF-1: ~20 μm and ACF-2: ~5 μm).

Hsa_circ_0008673 Promotes Breast Cancer Progression by MiR-578/GINS4 Axis.

Background: Circular RNAs (circRNAs) play a crucial role in breast cancer (BC) development. This study aimed to explore the new potential mechanism of hsa_circ_0008673 in BC.

Materials And Methods: Hsa_circ_0008673, microRNA-578 (miR-578) and recombinant human GINS complex subunit 4 (GINS4) abundances were measured via quantitative real-time PCR or western blot.

Quantitative Determination of Four Potential Genotoxic Impurities in the Active Pharmaceutical Ingredients in TSD-1 Using UPLC-MS/MS.

A novel method of ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for the identification and quantification of four potential genotoxic impurities (PGIs) in the active pharmaceutical ingredients of TSD-1, a novel P2Y12 receptor antagonist. Four PGIs were named, 4-nitrobenzenesulfonic acid, methyl 4-nitrobenzenesulfonate, ethyl 4-nitrobenzenesulfonate, and isopropyl 4-nitrobenzenesulfonate. Following the International Conference of Harmonization (ICH) guidelines, this methodology is capable of quantifying four PGIs at 15.

Learning-Based Rate Control for Video-Based Point Cloud Compression.

Due to limited transmission resources and storage capacity, efficient rate control is important in Video-based Point Cloud Compression (V-PCC). In this paper, we propose a learning-based rate control method to improve the rate-distortion (RD) performance of V-PCC. A low-latency synchronous rate control structure is designed to reduce the overhead of pre-coding.

Low-Complexity Error Resilient HEVC Video Coding: A Deep Learning Approach.

Intra/inter switching-based error resilient video coding effectively enhances the robustness of video streaming when transmitting over error-prone networks. But it has a high computation complexity, due to the detailed end-to-end distortion prediction and brute-force search for rate-distortion optimization. In this article, a Low Complexity Mode Switching based Error Resilient Encoding (LC-MSERE) method is proposed to reduce the complexity of the encoder through a deep learning approach.

A structural and functional analysis of Nna1 in Purkinje cell degeneration (pcd) mice.

The axotomy-inducible enzyme Nna1 defines a subfamily of M14 metallocarboxypeptidases, and its mutation underlies the Purkinje cell degeneration (pcd) mouse. However, the relationship among its catalytic activity, substrate specificities, and the critical processes of neurodegeneration/axon regeneration is incompletely understood. Here we used a transgenic rescue strategy targeting expression of modified forms of Nna1 to Purkinje cells in pcd mice to determine structure-activity relationships for neuronal survival and in parallel characterized the enzymatic properties of purified recombinant Nna1.

The carboxypeptidase-like substrate-binding site in Nna1 is essential for the rescue of the Purkinje cell degeneration (pcd) phenotype.

The Purkinje cell degeneration (pcd) phenotype is characterized by adult onset neurodegeneration resulting from mutations in Nna1, a gene encoding an intracellular protein with a putative metallocarboxypeptidase domain. As Nna1 is also induced in axotomized motor neurons, the elucidation of its function can shed light on previously unsuspected mechanisms common to degenerative and regenerative responses. Structural modeling revealed that Nna1 and three related gene products constitute a new subfamily of metallocarboxypeptidases with a distinctive substrate-binding site.

The Purkinje cell degeneration (pcd) mouse: an unexpected molecular link between neuronal degeneration and regeneration.

The spontaneous autosomal recessive mouse mutation, Purkinje cell degeneration (pcd), was first identified through its ataxic behavior. Since its discovery in the 1970s, the strain has undergone extensive investigation, although another quarter century elapsed until the mutant gene (agtpbp1 a.k.

Identification of candidate Purkinje cell-specific markers by gene expression profiling in wild-type and pcd(3J) mice.

The identification of mRNAs that have restricted expression patterns in the brain represents powerful tools with which to characterize and manipulate the nervous system. Here, we describe a strategy using microarray technology (Affymetrix Mouse Genome 430 2.0 Arrays) to identify mRNA transcripts that are candidate markers of cerebellar Purkinje neurons.