Base editors in zebrafish: a new era for functional genomics and disease modeling.

Base editing has revolutionized genome engineering by enabling precise single-nucleotide modifications without inducing double-strand breaks. As a powerful and efficient gene-editing tool, base editors (BEs) have been widely applied in various model organisms, including zebrafish (), to facilitate functional genomic studies and disease modeling. Zebrafish, with its genetic similarity to humans and rapid development, provides an excellent platform for testing and optimizing emerging base editing technologies.

Exploiting the Modulation Effects of Epitaxial Vanadium Film in a Quasi-BIC-Based Terahertz Metamaterial.

Terahertz (THz) metamaterials based on phase-change materials (PCMs) offer promising approaches to the dynamic modulation of electromagnetic responses. In this study, we design and experimentally demonstrate a tunable THz metamaterial composed of a symmetric split-ring resonator (SRR) pair, with the left halves covered by a 35 nm thick epitaxial vanadium dioxide (VO) film, enabling the simultaneous exploitation of both permittivity- and conductivity-induced modulation mechanisms. During the metal-insulator transition (MIT) of VO, cooperative changes in permittivity and conductivity lead to the excitation, redshift, and eventual disappearance of a quasi-bound state in the continuum (QBIC) resonance.

New insights into Gremlin-1: A tumour microenvironment landscape re-engineer and potential therapeutic target.

Gremlin-1 (GREM1), a well-known bone morphogenetic protein (BMP) antagonist, is highly expressed in various malignant tumours. However, the specific role of GREM1 in tumours remains controversial and may be attributed to the heterogeneity and complexity of the tumour microenvironment (TME). It is currently believed that GREM1 regulates the complex landscape of the TME, primarily by antagonising BMP signalling or BMP-independent pathways.

The first specific probe for pyrrolidine with multifunction by the interaction mechanism of atomic economic reaction.

Pyrrolidine (PyD) has an important impact on the environment and human health. However, there is currently no method for trace detection of PyD. Here, we successfully designed diaminomethylene-4H-pyran (1) as the first specific fluorescent probe for PyD.

Fluorinated benzothiadiazole fluorescent probe based on ICT mechanism for highly selectivity and sensitive detection of fluoride ion.

Excessive fluoride ion (F) in the environment can affect health and even endanger life when ingested by the human body. However, most fluoride probes have the disadvantages of low sensitivity and long detection time. Herein, fluorescent probe 3a is successfully synthesized by linking two acetylenyltrimethylsilyl groups at both ends of the fluorinated benzothiadiazole core.

Candidate molecules as alternative nitric oxide donors with better antibacterial property against Escherichia coli and Staphylococcus aureus.

Aims: Four nitric oxide (NO) donors, S-nitrosoglutathione (GSNO), S-nitrosocysteine (CySNO), S-nitroso-N-acetylcysteine (SNAC), and 2-(2-S-nitroso propionamide) acetic acid (GAS) were prepared and their physicochemical characteristics were analyzed. Besides, the antibacterial properties of NO donors were investigated against Escherichia coli and Staphylococcus aureus.

Methods And Results: UV-visible absorption spectrum and Fourier transform infrared spectrum verified the successful preparation of RSNOs.

Trace water in a BF·OEt system: a facile access to sulfinyl alkenylsulfones from alkynes and sodium sulfinates.

A highly efficient and operationally simple method for the synthesis of β-sulfinyl alkenylsulfones through a BF·OEt-promoted reaction of alkynes and sodium sulfinates is developed, successfully avoiding the complicated anhydrous treatment before the reaction and greatly simplifying the reaction conditions. As a facile and selective route to the targets, it features good functional group compatibility, mild conditions, easily available starting materials, and excellent yields. Notably, the trace water in solvent plays a key role in promoting the reaction, which provides a more practical pathway for the utilization of the BF·OEt catalytic system.

Fine-tuning benzazole-based probe for the ultrasensitive detection of Hg in water samples and seaweed samples.

Developing potentially toxic metal ion probes is significant for environment and food safety. Although Hg probes have been extensively studied, small molecule fluorophores that can integrate two applications of visual detection and separation into one unit remain challenging to access. Herein, by incorporating triphenylamine (TPA) into tridentate skeleton with an acetylene bridge, 2,6-bisbenzimidazolpyridine-TPA (4a), 2,6-bisbenzothiazolylpyridine-TPA (4b) and 2,6-bisbenzothiazolylpyridine-TPA (4c) were first constructed, expectably showing distinct solvatochromism and dual-state emission properties.

PatchNet: Maximize the Exploration of Congeneric Semantics for Weakly Supervised Semantic Segmentation.

With the increase in the number of image data and the lack of corresponding labels, weakly supervised learning has drawn a lot of attention recently in computer vision tasks, especially in the fine-grained semantic segmentation problem. To alleviate human efforts from expensive pixel-by-pixel annotations, our method focuses on weakly supervised semantic segmentation (WSSS) with image-level labels, which are much easier to obtain. As a considerable gap exists between pixel-level segmentation and image-level labels, how to reflect the image-level semantic information on each pixel is an important question.

Regulating donor-acceptor system toward highly efficient dual-state emission for sensitive response of nitroaromatic explosives.

Dual-state emission luminogens (DSEgens) as fluorophores emit efficiently in solution and solid forms have gained increasing concern in the field of chemical sensing. Recent efforts by our group led to the identification of DSEgens as an easy-to-visualize nitroaromatic explosives (NAEs) detection platform. However, none of the previously studied NAEs probes show effective improvement in sensitivity.

Multivariate models of commodity futures markets: a dynamic copula approach.

We apply flexible multivariate dynamic models to capture the dependence structure of various US commodity futures across different sectors between 2004 and 2022; particular attention is paid to the 2008 financial crisis and the COVID-19 pandemic. Our copula-based models allow for time-varying nonlinear and asymmetric dependence by integrating elliptical and skewed copulas with dynamic conditional correlation (DCC) and block dynamic equicorrelation (Block DECO). Flexible copula models that allow for multivariate asymmetry and tail dependence are found to provide the best performance in characterizing co-movements of commodity returns.

The emerging therapeutic target of dynamic and reversible N6-methyladenosine modification during cancer development.

As a reversible and dynamic epigenetic modification, N6-methyladenosine (m6A) modification is ubiquitous in eukaryotic cells. m6A methylation is prevalent in almost all RNA metabolism processes that affect the fate of cells, including cancer development. As indicated by the available evidence, targeting m6A regulators may play a crucial role in tumor therapy and multidrug resistance.

The Medical Segmentation Decathlon.

International challenges have become the de facto standard for comparative assessment of image analysis algorithms. Although segmentation is the most widely investigated medical image processing task, the various challenges have been organized to focus only on specific clinical tasks. We organized the Medical Segmentation Decathlon (MSD)-a biomedical image analysis challenge, in which algorithms compete in a multitude of both tasks and modalities to investigate the hypothesis that a method capable of performing well on multiple tasks will generalize well to a previously unseen task and potentially outperform a custom-designed solution.

An investigation on catalytic nitrite reduction reaction by bioinspired Cu complexes.

Catalytic nitrite reductions by Cu complexes containing anionic Tp, neutral Tpm, or neutral TIC ligands in the presence of L-ascorbic acid, which served as an electron donor and proton source, were investigated. The results showed that auxiliary ligands are important for copper-mediated catalytic nitrite reduction. Furthermore, the electronic effects of the ligand govern the nitrite reduction efficiency, which should be considered at two control points: one is the susceptibility of the LCu-nitrite species to protonation and the other is the susceptibility of LCu to reduction giving LCu.

Epigenetic enzyme mutations as mediators of anti-cancer drug resistance.

Despite the rapid advancement in the introduction of new drugs for cancer therapy, the frequent emergence of drug resistance leads to disease progression or tumor recurrence resulting in dismal prognosis. Given that genetic mutations are thought to be important drivers of anti-cancer drug resistance, it is of paramount importance to pin-point mutant genes that mediate drug resistance and elucidate the underlying molecular mechanisms in order to develop novel modalities to surmount chemoresistance and achieve more efficacious and durable cancer therapies. Cumulative evidence suggests that epigenetic alterations, especially those mediated by epigenetic enzymes with high mutation rates in cancer patients, can be a crucial factor in the development of chemoresistance.

Adversarial Learning With Cost-Sensitive Classes.

It is necessary to improve the performance of some special classes or to particularly protect them from attacks in adversarial learning. This article proposes a framework combining cost-sensitive classification and adversarial learning together to train a model that can distinguish between the protected and unprotected classes, such that the protected classes are less vulnerable to adversarial examples. We find in this framework an interesting phenomenon during the training of deep neural networks, called the Min-Max property, that is, the absolute values of most parameters in the convolutional layer approach 0 while the absolute values of a few parameters are significantly larger, becoming bigger.

A programmable and skin temperature-activated electromechanical synergistic dressing for effective wound healing.

Mechanical regulation and electric stimulation hold great promise in skin tissue engineering for manipulating wound healing. However, the complexity of equipment operation and stimulation implementation remains an ongoing challenge in clinical applications. Here, we propose a programmable and skin temperature-activated electromechanical synergistic wound dressing composed of a shape memory alloy-based mechanical metamaterial for wound contraction and an antibacterial electret thin film for electric field generation.

Rational Design and Facile Synthesis of Dual-State Emission Fluorophores: Expanding Functionality for the Sensitive Detection of Nitroaromatic Compounds.

Six novel benzimidazole-based D-π-A compounds 4 a-4 f were concisely synthesized by attaching different donor/acceptor units to the skeleton of 1,3-bis(1H-benzimidazol-2-yl)benzene on its 5-position through an ethynyl link. Due to the twisted conformation and effective conjugation structure, these dual-state emission (DSE) molecules show intense and multifarious photoluminescence, and their fluorescence quantum yields in solution and solid state can be up to 96.16 and 69.

A self-powered implantable and bioresorbable electrostimulation device for biofeedback bone fracture healing.

Electrostimulation has been recognized as a promising nonpharmacological treatment in orthopedics to promote bone fracture healing. However, clinical applications have been largely limited by the complexity of equipment operation and stimulation implementation. Here, we present a self-powered implantable and bioresorbable bone fracture electrostimulation device, which consists of a triboelectric nanogenerator for electricity generation and a pair of dressing electrodes for applying electrostimulations directly toward the fracture.

High-Affinity Sulfate Transporter Sultr1;2 Is a Major Transporter for Cr(VI) Uptake in Plants.

Chromate (Cr[VI]) is a highly phytotoxic contaminant that is ubiquitous in soils. However, how Cr(VI) is taken up by plant roots remains largely unknown. Here, we show that the high-affinity sulfate transporter Sultr1;2 is responsible for Cr(VI) uptake by the roots of .

Mechanical Terahertz Modulation by Skin-Like Ultrathin Stretchable Metasurface.

The modulation of terahertz plays a key role in realizing the tunable terahertz devices. The concept of flexible and stretchable electronics provides the possibility to dynamically modulate the terahertz with mechanical strain rather than additional electrical components. Here, the mechanical modulation of the terahertz transmission with a freestanding, skin-like, and highly stretchable metasurface is experimentally illustrated.

Self-Activated Electrical Stimulation for Effective Hair Regeneration a Wearable Omnidirectional Pulse Generator.

Hair loss, a common and distressing symptom, has been plaguing humans. Various pharmacological and nonpharmacological treatments have been widely studied to achieve the desired effect for hair regeneration. As a nonpharmacological physical approach, physiologically appropriate alternating electric field plays a key role in the field of regenerative tissue engineering.

Tailoring the energy band in flexible photodetector based on transferred ITO/Si heterojunction via interface engineering.

Interface engineering is an important method to modulate electronic structures for improving the physical properties of semiconductors as well as designing novel devices. Recently, development of flexible electronic devices based on inorganic thin films on flexible substrates, which provides solutions to meet the emerging technological demands, may also expend the methodology of interface engineering. Herein, a semitransparent photodetector based on an indium-tin oxide (ITO)-on-silicon (Si) heterojunction was fabricated on a flexible substrate and investigated under mechanical bending strains.