WATER NEWS: a field approach for sustainable detection of pathogens in wastewater.

Wastewater surveillance is a critical tool in responding to the COVID-19 pandemic, yet traditional centralized methods are unsustainable due to their high costs and complex implementation requirements. Here, we introduce Wastewater Analysis and Tracking for Epidemiological Recognition: Necessary Early Warning System (WATER NEWS)-a field clustered regularly interspaced short palindromic repeats-diagnostic approach designed for robust and cost-effective pathogen detection in wastewater treatment plants (WWTPs). WATER NEWS combined a one-pot assay, an optimized reporter probe, on-site nucleic acid extraction, an improved freeze-drying process and a user-friendly, low-cost, battery-operated device, thereby overcoming the limitations of field-deployable wastewater surveillance.

Case Report: A Chinese family with MYH9-RD caused by p.E1841K mutation exhibiting widespread may-hegglin inclusions.

Introduction: MYH9-related disease (MYH9-RD) is a rare autosomal dominant genetic syndrome characterized by congenital thrombocytopenia, with a risk of developing progressive nephropathy, sensorineural deafness, and presenile cataract. Due to its presentation of isolated thrombocytopenia, it is frequently misdiagnosed as immune thrombocytopenic purpura (ITP).

Methods: A 10-year-old girl with an initial diagnosis of ITP was evaluated, based on isolated thrombocytopenia and intermittent epistaxis.

Environmental policy and corporate green innovation: The role of penalties, taxes, and subsidies in China.

Environmental penalties (EP), environmental protection taxes (ET), and environmental subsidies (ES) are key components of China's environmental regulatory framework, yet their combined and individual effects on corporate green innovation (CGI) remain poorly understood. This study investigates the direct and interactive effects of these policy instruments using panel data from A-share listed firms between 2008 and 2021. We find that: (1) ET and ES significantly stimulate both substantive and strategic green innovation, while EP has a counterproductive effect on substantive innovation; (2) EP exacerbates corporate financing constraints, ET promotes environmental investments, and ES strengthens internal green governance practices, each influencing innovation outcomes differently; (3) There is a substitution effect between EP and ET, while no significant interaction is observed between other policy pairs; (4) Heterogeneity tests show that technology-intensive firms are more responsive to ET and ES, while non-technology-intensive firms are more sensitive to EP.

Endothelial cells secrete small extracellular vesicles to promote neuron endoplasmic reticulum stress injury via miR-146a-5p/Eif4g2 axis in ischemic stroke.

Introduction: Ischemic stroke (IS) ranks among the top global causes of mortality and disability, with existing treatments proving inadequate because of its complex pathological process. Cell communication mediated by extracellular vesicle (EV)-containing miRNAs between vascular endothelial cells and neurons is essential for IS formation.

Objectives: This research sought to explore the effects and molecular mechanisms of endothelial cell EV-derived miR-146a-5p on neuron injury in IS.

Development and validation of an innovative approach to estimating FVIII levels and designing personalised doses in the prophylactic treatment of haemophilia A: based on the principle of the two-compartment model.

Objective: To predict the level at a specified time and design personalised dosing, we proposed a method (Method 1) for estimating coagulation factor VIII (FVIII) pharmacokinetic (PK) parameters using 4 levels within 48 hours after administering a test dose.

Design: A retrospective study based on virtual populations and real patients.

Setting: A comprehensive hospital in China.

Circulating mucosa-associated invariant T cells are decreased and have impaired function in patients with diffuse large B-cell lymphoma.

Mucosa-associated invariant T (MAIT) cells are associated with tumor immunity. However, their role in diffuse large B-cell lymphoma (DLBCL) remains unclear. Therefore, this study aimed to elucidate frequency and functional characteristics of circulating MAIT cells in DLBCL patients.

The Multi-Dimensional Role of Vitamin D in the Pathophysiology and Treatment of Diabetic Foot Ulcers: From Molecular Mechanisms to Clinical Translation.

Diabetic foot ulcers (DFUs) constitute a severe and debilitating complication of diabetes, imposing a substantial global health burden due to their intricate pathophysiology and impaired wound healing processes. Vitamin D deficiency is highly prevalent among diabetic populations, and accumulating evidence indicates its potential involvement in the pathogenesis and prognosis of DFUs. This review comprehensively explores the diverse roles of vitamin D in DFUs, encompassing its molecular mechanisms such as immunomodulation, promotion of angiogenesis, neuroprotection, and induction of antimicrobial peptides, as well as the metabolic characteristics associated with various vitamin D forms and compromised vitamin D receptor (VDR) signaling pathways.

Terrestrial Nocturnal Roosting Behavior of Black-necked Cranes () on the Yunnan-Guizhou Plateau: Active Choice or Forced Environmental Adaptation.

Nocturnal roosting sites are integral to bird habitats, with their use and selection by birds serving as indicators of behavioral adaptations to environmental pressures. Black-necked Cranes (), which typically roost in shallow water, have exhibited an unexpected "terrestrialization" of nocturnal roosting sites within their eastern wintering population of southwest China. Despite this phenomenon being documented since the late 20th century, research on terrestrial nocturnal roosting behavior remains limited, hindered by technological challenges.

AI-based pelvic floor surface electromyography reference ranges and high-precision pelvic floor dysfunction diagnosis.

Background: Pelvic floor surface electromyography (sEMG) is widely used to evaluate and treat pelvic floor dysfunctions (PFDs). Based on sEMG, the Glazer protocol was developed over 20 years ago with a limited sample size, making it challenging to accurately diagnose PFDs across diverse populations and conditions. This study aims to establish a multidimensional database for monitoring pelvic floor sEMG, derive more reasonable reference ranges for sEMG parameters, and achieve accurate diagnosis of PFDs through artificial intelligence (AI).

FB1 causes barrier damage to vascular endothelial cells through ferroptosis by a PINK1/Parkin mediated mitophagy-dependent mechanism.

Fumonisin B1 (FB1) is an environmental mycotoxin produced mainly by fungi of the genus Fusarium. Exposure to FB1 can lead to pulmonary edema in pigs, likely caused by damage to vascular endothelial cells, but the mechanism of FB1-induced damage was unknown. Here, we found that FB1 damages vascular endothelial cells through ferroptosis, marked by iron-dependent membrane lipid peroxidation, and through mitophagy, a selective autophagy that targets mitochondria.

Construction and Immune Strategy Optimization of a Vaccine Strain for Influenza A (H5N8) Subtype.

Multiple subtypes of avian influenza virus (AIV), including H5N1, H5N6, and H5N8 viruses, are currently co-circulating in wild birds and poultry and causing sporadic human infections. Vaccine development is essential for pandemic preparedness. In this study, we constructed a candidate vaccine virus (CVV) using reverse genetics (RG) based on the sequence of the first human-infected H5N8 subtype AIV, A/Astrakhan/3212/2020 (H5N8).

Comprehensive pan-cancer analysis identifies PLAG1 as a key regulator of tumor immune microenvironment and prognostic biomarker.

Background: The literature on the role of pleomorphic adenoma gene 1 (PLAG1) in malignant tumors is limited. This study aimed to perform pan-cancer analysis of PLAG1.

Methods: The expression of PLAG1 was analyzed by Human Protein Atlas (HPA).

Abnormal proportions and functions of myeloid-derived suppressor cells in peripheral blood of patients with diffuse large B-cell lymphoma.

Background: Myeloid-derived suppressor cells (MDSCs) are a subset of immature myeloid cells with immunosuppressive properties. Evidence suggests that abnormal immune system can lead to immune dysfunction and increase the risk of developing diffuse large B-cell lymphoma (DLBCL). This study investigated the abnormality of MDSCs in the peripheral blood of patients with DLBCL.

A microprotein encoded by LINC00263 promotes breast cancer osteolytic bone metastasis by inducing osteoclastogenesis and inhibiting osteoclast ferroptosis.

Currently, there are no effective prevention or therapeutic methods for breast cancer bone metastasis (BC-BM), which leading to severe skeletal complications and increased mortality. Understanding the mechanisms underlying BC-BM could provide potential strategies for its prevention and treatment. In this study, we identified a new microprotein encoded by lncRNA LINC00263, which we named LINC00263-encoded protein (LINC00263-P), was significantly upregulated in bone metastatic breast cancer tissues and correlated with BC-BM.

Fumonisin B1 induces oxidative stress, inflammation and necroptosis in IPEC-J2 cells.

Fumonisin B1 (FB1), an important mycotoxin, poses a significant threat to public health and livestock production due to its widespread contamination. Furthermore, the gastrointestinal tract is particularly vulnerable to FB1 exposure given its frequent contamination of staple crops such as corn. Although necroptosis has been recognized as a critical mechanism underlying intestinal damage caused by certain environmental toxins, whether FB1 specifically triggers necroptosis in intestinal epithelial cells remains to be fully elucidated.

A pumpkin heat shock factor CmHSF30 positively regulates thermotolerance in transgenic plants.

Heat shock factors (HSFs) play a central role in regulating the responses of plants to various stresses. However, the function and regulation of HSFs in pumpkins remains largely unknown. In this study, an HSF, CmHSF30 was identified in Cucurbiamoschata, which belongs to the HSFA subfamily.

Study on hangfire failure mechanism in firearm firing-ignition systems.

To investigate the hangfire failure mechanism in firearm firing-ignition systems, gas pressure responses were experimentally analyzed using a firing-ignition simulation test device. Pressure characteristic parameters were statistically evaluated using the "3σ" criterion to establish the hangfire failure thresholds. A finite element model simulating the mechanical-thermal-chemical multi-mechanism coupling process during primer mixture ignition was developed and validated against experimental results.

Proteasome inhibition induces DNA methylation alteration by attenuating the synthesis of DNA methyltransferase 1 and 3B in colorectal cancer.

Proteasome is an essential organelle responsible for maintaining cellular protein homeostasis, but its relationship with DNA methylation remains unknown. In this study, we assessed DNA methylation of colorectal cancer (CRC) cells following treatment with proteasome inhibitors, and investigated the underlying mechanism of DNA methylation changes and the biological effects on CRC cells. We established that inhibition of proteasome leads to significant alterations in DNA methylation profile in CRC by suppressing the synthesis of DNA methyltransferases (DNMTs).

RBMS3-loss impedes TRIM21-induced ubiquitination of ANGPT2 in an RNA-independent manner and drives sorafenib resistance in hepatocellular carcinoma.

Sorafenib, a first-line targeted drug for advanced hepatocellular carcinoma (HCC), has limited clinical application due to intrinsic/acquired resistance. In this study, we have identified the RNA-binding protein RBMS3 as a pivotal regulator involved in sorafenib resistance among patients with HCC. Loss- and gain-of-function experiments further demonstrate that downregulation of RBMS3 promotes angiogenesis and confers resistance to sorafenib by augmenting the capacity of HCC cells to express and secrete ANGPT2, while upregulation of RBMS3 reverse these phenotypes.

Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection.

The CRISPR-based detection methods have been widely applied, yet they remain limited by the non-universal nature of one-pot diagnostic approaches. Here, we report a universal one-pot fluorescent method for the detection of epidemic pathogens, delivering results within 15-20 min. This method uses heparin sodium to precisely tunes the cis-cleavage capability of Cas12 via interference with the Cas12a-crRNA binding process, thereby generating significant fluorescence due to the accumulation of isothermal amplification products.

SQSTM1/p62 predicts prognosis and upregulates the transcription of CCND1 to promote proliferation in mantle cell lymphoma.

Mantle cell lymphoma (MCL) is a rare, highly invasive non-Hodgkin's lymphoma. The main pathogenesis of MCL is associated with the formation of the IgH/CCND1 fusion gene and nuclear overexpression of cyclin D1, which accelerates the cell cycle, leading to tumorigenesis. The prognosis with current standard chemotherapy is still unsatisfactory.

Thyroid cancer survival and prognostic factors in Yantai, China (2012-2022): a population-based study.

Background: Although thyroid cancer is associated with low mortality rates, significant racial disparities in thyroid cancer outcomes have not been adequately studied in Asia. Moreover, the Asian population consists of different ethnic groups that are not homogeneous. This study aimed to perform a population-based analysis of survival outcomes and prognostic factors in thyroid cancer patients.

A pan-cancer analysis of the oncogenic role of N-acetyltransferase 8 like in human cancer.

Background: N-Acetyltransferase 8 Like (NAT8L) inhibits natural killer (NK)/T-cell cytotoxicity by impairing the formation of the immunological synapse via N-acetylaspartate (NAA). Existing research has predominantly focused on the metabolic functions of NAT8L, particularly in adipose tissues and myelination in the brain. However, in contrast to other N-acetyltransferases such as NAT1 and NAT2, the role of NAT8L in cancer has been less extensively studied.