Resolution assessment of super-resolution microscopy imaging: structural and technical dependencies for cell biology.

Super-resolution fluorescence microscopy (SRM) has enabled visualization of nanoscale cellular structures, but systematic evaluation of resolution assessment methods across diverse biological structures and SRM modalities remains lacking. Here, we comparatively assessed three resolution metrics-Full Width at Half Maximum (FWHM), decorrelation analysis, and Fourier Ring Correlation (FRC)-across two SRM techniques (Super-resolution Radial Fluctuation, SRRF; Stimulated Emission Depletion, STED) using key subcellular structures: microtubules (filaments), mitochondria (membranes), and nuclear pore protein Nup98 (single particles) in HeLa/U2OS cells. Our results showed decorrelation analysis provided robust resolution estimates across all structures and modalities (confocal/SRRF/STED), exhibiting superior performance for dense nuclear pore complexes where FWHM failed due to overlapping point spread functions.

Cloud-integrated microfluidic fluorescence sensing platform using UCNP/FeO@Cu nanoprobes for simultaneous and on-site multi-pesticide residue detection with smartphone assistance.

Rapid and efficient detection of pesticide residues is essential for ensuring food safety. Herein, we develop a smartphone-assisted multi-channel microfluidic paper-based device (MCPD) biosensor integrated with a cloud platform for the simultaneous detection of glyphosate (Gly), malathion (Mal) and acetamiprid (Ace). Detection zones are functionalised using nanoprobes fabricated via coupling up-conversion nanoparticles (UCNPs) with FeO@Cu.

Parameter optimization of NanoJ-SRRF for live-cell microtubule imaging.

Super-Resolution Radial Fluctuation (SRRF) enables live-cell super-resolution imaging, but requires careful parameter selection. Here, we quantify the impact of NanoJ-SRRF parameters on microtubule imaging using FWHM and SQUIRREL-based error mapping. Ring radius proved most critical, with values >1.

Risk factors for infections in systemic lupus erythematosus: a meta-analysis.

Immune dysfunction in patients with systemic lupus erythematosus (SLE) increases susceptibility to infections, complicating their management. Identifying risk factors for infections is essential for improving clinical outcomes. A meta-analysis was conducted to evaluate factors associated with infection risk in patients with SLE.

Glycoproteomics Analysis to Identify Potential Biomarkers and Investigate CD14 Monocyte Activation Mechanisms via Exosomal Proteins in Cerebral Infarction Using a Novel Glycogen-Functionalized Nanoprobe.

Cerebral infarction (CI) is a leading cause of disability and mortality, with activated plasma monocytes and altered protein glycosylation identified as critical contributors to its pathology. However, the mechanisms linking peripheral monocyte activation and glycoproteins in CI remain inadequately understood. In this study, we developed a glycogen-functionalized nanoprobe (mSiO@FeO@Glycogen) to profile plasma N-glycosylated proteins in healthy controls, CI patients, and patients in the rehabilitation phase (CIR).

Chemotherapeutic drug scavenger-based combination therapy toward treating triple-negative breast cancer.

Triple-negative breast cancer (TNBC) is resistant to most antitumor treatments, leaving chemotherapy as the primary option. Although doxorubicin (Dox) in combination with other therapies is promising for TNBC management, the combined effect is still compromised by the dose-limiting toxicities of Dox. Here, we developed a chemotherapeutic drug scavenger (CDS) by encapsulating GC-rich DNA-preferred binding targets of Dox-within an erythrocyte membrane functionalized with a normal tissue-targeting (NTT) peptide.

Research progress on the mechanisms of pain empathy.

Recent research has highlighted the indispensability of traditional molecular biology and imaging techniques in pain research. However, the mechanisms underlying pain empathy remain unclear. Consequently, a deeper understanding of these mechanisms would greatly enhance pain management.

Copper(I)-Catalyzed Synthesis of 1-Amino-1,3-diene Derivatives through a Formal 1,3-Amino Migration Process.

We present a highly efficient method utilizing a CuBr/DTBP (di--butyl peroxide) catalyst system for the synthesis of 1-amino-1,3-diene derivatives from propargylamines and activated methylene compounds. This transformation efficiently assembles a wide range of substrates with good functional group tolerance and high atomic efficiency. Mechanistic investigations reveal that this reaction proceeds through a copper-catalyzed C-C bond formation followed by a formal 1,3-amino group migration.

Super-resolution radial fluctuations (SRRF): a versatile and accessible tool for live-cell nanoscopy.

Super-resolution radial fluctuation (SRRF) microscopy is a novel computational imaging technique that bypasses the optical diffraction limit (lateral resolutions of 200-300 nm), achieving lateral resolutions of approximately 50-100 nm while being compatible with live-cell imaging. Unlike traditional super-resolution methods such as stimulated emission depletion (STED) and single molecule localization microscopy (SMLM), SRRF minimizes phototoxicity and hardware complexity by analyzing fluorescence intensity fluctuations in standard wide-field microscopy data. This is achieved by calculating local gradient convergence ("radiality") across time-series images, enabling the reconstruction of sub-diffraction structures without specialized fluorophores or high-intensity illumination.

Enhanced Activity and Thermal Stability of Hydroxyl-Modified Bis(imino)pyridylcobalt Catalysts for Ethylene Polymerization.

Bulky N-aryl substituents in bis(imino)pyridylmetal catalysts enhance the ethylene polymerization, though maintaining activity at higher temperature remains a challenging point. Herein, the newly synthesized symmetrical and unsymmetrical 2-(1-(2,6-dibenzhydryl-4-hydroxyphenylimino)ethyl)-6-(1-(arylimino)ethyl)pyridylcobalt chloride complexes have been systematically and sterically tailored and further explored for their performances in ethylene polymerization. Employing the bulky N-aryl unit modified through dibenzhydryl and hydroxyl substituents, the cobalt complexes show a significant improvement in both activity and thermal stability.

Removal of emerging organic contaminants in a subsurface wastewater infiltration system: A preliminary study of microbial mechanism.

Subsurface wastewater infiltration systems (SWISs) have been widely used for rural decentralized wastewater treatment, but their performance in removing emerging organic contaminants (EOCs) from wastewater remains unclear. We investigated EOC removal and related microbial mechanism in an intermittently aerated SWIS operated at a hydraulic loading rate of 0.5 m/m/day under field conditions.

Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections.

Antivirulence vaccination represents a promising strategy for infection prevention, but achieving both safety and efficacy in toxoid vaccine preparation remains a challenge. Cell membrane-based nanotoxoids offer a safe delivery platform for bacterial virulence factors in antivirulence vaccination, but limited absorption capacity hampers their efficacy. Here, we develop a lipid-based toxoid vaccine platform, PSV-CNP, comprising a CpG-loaded polymeric core coated with phosphatidylcholine/sphingomyelin (PS) liposomes enriched with bacterial virulence factors.

Short Videos Turn Everyone into Bearers of Traditional Sports and Games: A Mixed-Methods Study from China.

The emergence of short video applications ("apps") has facilitated the dissemination, inheritance, and protection of traditional sports and games (TSGs). However, the effectiveness of these apps in raising public awareness and responsibility toward the preservation and heritage of TSGs has received insufficient research attention. This study constructs a theoretical model based on value-belief-norm theory and the theory of planned behavior, employing both PLS-SEM and fsQCA methods to empirically analyze 417 questionnaires.

Astrocytic mGluR5-dependent calcium hyperactivity promotes amyloid-β pathology and cognitive impairment.

Astrocytic dysfunction is a crucial factor for the pathogenesis of Alzheimer's disease. Metabotropic glutamate receptor 5 (mGluR5) is ubiquitously expressed in the brain and is a key molecule that regulates synaptic transmission and plasticity. It has been shown that mGluR5 is elevated in astrocytes in Alzheimer's disease.

Seismic performance analysis of braced steel structures based on vibration experiments and finite element simulation.

The study analyzes the seismic performance of spatial steel frame structures by creating a three-dimensional nonlinear finite element model and designing vibration table model experiments. This is done to ensure the seismic performance of the steel framing-bracing system in spatial steel frame structures and to raise the level of seismic and disaster prevention technology of steel structures. The outcomes revealed that under the experimental load consisting of simulated seismic waves generated by the shaker, the initial natural frequencies of the bidirectional bracing arrangement in white noise in the X and Y-directions were larger, 53.

Engineering Spores to Display Nicotine Oxidase: In Situ Specific and Sensitive Nicotine Detection.

Nicotine exposure poses a significant public health challenge, necessitating highly sensitive and real-time detection methods. This study presents a novel live electrochemical biosensor (EN@FeSe@MHCS/GCE) by integrating synthetic biology and materials engineering. A genetically engineered NOX mutant-with 9.

Sorption and mechanisms of legacy and emerging per- and polyfluoroalkyl substances (PFASs) on different particle size fractions of marine sediments.

PFASs are ubiquitous in various environmental and biological media due to their extensive application and stability. However, the sorption of PFASs, especially emerging PFASs, on different particle size fractions of marine sediments remains unknown. Here, we investigated the sorption kinetics, isotherms, and mechanisms of six legacy and emerging PFASs on five different particle size fractions of marine sediments (F1 (69.

Treating and Protecting against Recurrent Vulvovaginal Candidiasis Using the Vaginal Epithelial Cell Membrane-Based Photoimmunotherapeutic Nanoplatform.

Recurrent vulvovaginal candidiasis (RVVC) is an opportunistic infection predominantly caused by () and is particularly prevalent among individuals on immunosuppressants. Currently, there are no FDA-approved therapies for specifically controlling RVVC, mainly due to the need for therapeutics against RVVC that require both antifungal treatments to resolve active infections and strategies to prevent recurrence. This study introduces a biomimetic photoimmunotherapeutic nanoplatform consisting of an adjuvant-encapsulated polymeric core stabilized by a photosensitizer-loaded vaginal epithelial cell membrane coating to treat and protect against RVVC.

Meplazumab, a CD147 antibody, for severe COVID-19: a double-blind, randomized, placebo-controlled, phase 3 clinical trial.

Meplazumab, a humanized CD147 antibody, showed favorable safety and clinical benefits in phase 1 and phase 2/3 seamless clinical studies. Further evaluation of its therapeutic efficacy in patients with severe COVID-19 is needed. In this phase 3 add-on study, we randomized patients with severe COVID-19 in a 1:1 ratio to receive 0.

Computer-Aided Drug Design in Research on Chinese Materia Medica: Methods, Applications, Advantages, and Challenges.

Chinese materia medica (CMM) refers to the medicinal substances used in traditional Chinese medicine. In recent years, CMM has become globally prevalent, and scientific research on CMM has increasingly garnered attention. Computer-aided drug design (CADD) has been employed in Western medicine research for many years, contributing significantly to its progress.

Sub pulse length event measurement in BOTDR system using slope assisted Brillouin frequency shift.

To overcome the limitations by pulse width on the event zone measurement capability, a novel temperature demodulation scheme was proposed and validated to measure sub-pulse length event zones based on the slope-assisted Brillouin frequency shift (SA-BFS). Unlike the conventional BFS-based methods, this approach leverages the BFS slope for temperature demodulation, enabling precise measurement of sub-pulse-length event zones. We developed a theoretical model that established a linear relationship between the BFS slope and temperature, validated through simulations and experiments across various event zone lengths and pulse widths.

Population genetic structure and historical demography of Saccostrea echinata in the Northern South China sea and Beibu Gulf.

Marine invertebrates, such as oysters, were once thought to form large, panmictic populations with little genetic differentiation due to their high reproductive capacity and dual life stages. However, recent studies have shown significant genetic structuring and moderate gene flow across populations, influenced by factors like ocean currents, historical climate events, and environmental changes. The black-lip oyster (Saccostrea echinata), with its extensive dispersal potential, is ideal for population genetics studies.

Aquatic plants dominate spatiotemporal dynamics of NO fluxes in small urban lake by regulating nutrient distribution and emission path.

Small urban lakes are recognized as significant sources of nitrous oxide (NO) to the atmosphere. Despite the crucial role of aquatic plants in landscape construction and pollutant removal within urban lakes, the modulation of NO emission dynamics and associated mechanisms by these plants remains elusive. This study investigated the NO concentrations and fluxes from aquatic habitats covered with seven species of aquatic plants in a small urban lake, and estimated the contribution of plant-mediated NO emissions.

Ultrasensitive detection of chloramphenicol using a performance-enhanced Fe/Zn-MIL-88B driven dual-mode biosensing platform.

Chloramphenicol (CAP) used in aquaculture poses a great threat to human health. In this study, a novel biosensing platform was proposed by Fe/Zn-MIL-88B with performance-enhanced for fluorescent/colorimetric dual-mode detection of CAP. Zinc was incorporated into the metal-organic framework to modulate its internal electronic structure, resulting in the formation of Fe/Zn-MIL-88B.

Biomimetic rhCOL17-P334 Conjugate for Enhanced Wound Healing.

Wound healing remains a significant global health challenge, affecting millions annually and imposing substantial economic burdens. Most commercially available biomaterials for wound management primarily address external symptoms, including hemostasis, exudation, scarring, and infection. Advanced biomaterials derived from endogenous molecules aim to better replicate the native wound microenvironment, promoting enhanced repair.