Mechanistic Insights into Recovery of Partial Denitrification/Anammox under Continuous Flow: Balancing Nitrite Supply and Microbial Competition.

Partial denitrification coupled with anammox (PD/A) has emerged as a promising low-carbon strategy for energy-efficient nitrogen removal from municipal wastewater. However, the reactivation of PD/A systems following operational disturbances remains challenging, particularly under continuous-flow conditions, where microbial interactions and process stability are more complex than in sequencing batch reactors. This study systematically and first evaluated the recovery dynamics of a continuous-flow PD/A process seeded with low-activity granular sludge stored at 4 °C for three months.

Transcriptome Analysis of Tear-Derived Extracellular Vesicles as Biomarkers for Diabetic Retinopathy.

Diabetic retinopathy (DR) represents the predominant etiology of visual impairment and blindness on a global scale. At present, there is still a dearth of robust biomarkers for DR, which substantially limits its diagnosis, particularly at an early stage. Tears, owing to their inherent proximity to the ocular microenvironment, offer a unique advantage as a source of potential biomarkers for retinal pathologies.

Surveillance of tick-borne viruses in the border regions of the Tumen River Basin: Co-circulation in ticks and livestock.

Background: The unique eco-geographical patterns and climatic conditions of the China-Tumen River border region, combined with frequent cross-border tourism and trade activities, collectively establish this area as a recognized hotspot for tick-borne disease outbreaks. However, critical knowledge gaps persist regarding the eco-epidemiology of emerging tick-borne viruses and the distribution of their potential reservoir hosts within this trinational ecosystem spanning China, North Korea, and Russia.

Methods: We collected a total of 2,004 ticks from the study area, along with blood samples obtained from 42 sheep and 45 cattle.

Promotion of Bone Defect Repair Using Decellularized Antler Cancellous Bone Loaded with Deer Osteoglycin.

The combination of scaffold materials and bioactive factors is a promising strategy for promoting bone defect repair in tissue engineering. Previous studies have shown that osteoglycin (OGN) is highly expressed in the bone repair process using deer antler as an animal model of bone defects. It suggests that OGN may be a key active component involved in the bone repair process.

Mechanisms of action of traditional Chinese medicine in the treatment of myocardial injury: A comprehensive review.

Background: Myocardial Injury (MI) is a condition in which there is an adverse reaction to the function of the heart, which can seriously damage health in some severe cases, producing symptoms such as shock and sudden death. The causes of MI are varied, as are the methods of recovery. To pursue a faster therapeutic effect, western drugs with antiviral and antiplatelet similar effects are usually adopted in clinical practice, but such drugs mostly have side effects including allergic reaction, liver function damage, drug dependence and so on.

Deep Learning-Enabled Ultrasound for Advancing Anterior Talofibular Ligament Injuries Classification: A Multicenter Model Development and Validation Study.

Rationale And Objectives: Ultrasound (US) is the preferred modality for assessing anterior talofibular ligament (ATFL) injuries. We aimed to advance ATFL injuries classification by developing a US-based deep learning (DL) model, and explore how artificial intelligence (AI) could help radiologists improve diagnostic performance.

Materials And Methods: Consecutive healthy controls and patients with acute ATFL injuries (mild strain, partial tear, complete tear, and avulsion fracture) at 10 hospitals were retrospectively included.

Therapeutic potential of Chinese herbal medicine for coronary heart disease patients with cerebral ischemic stroke: a systematic review and meta-analysis.

Objective: This research sought to demonstrate potential therapeutic strategies for coronary heart disease (CHD) patients with cerebral ischemic stroke (CIS) by rigorously evaluating the efficacy and safety of Chinese Herbal Medicine (CHM) through meta-analysis.

Methods: A broad search approach was applied to obtain pertinent articles from both domestic and international databases, covering publications up to 31 December 2024. Using RevMan software (Version 5.

Association between coffee intake and serum α-Klotho levels in adults: a population-based study.

Background: Coffee is widely consumed in the US, linked to various health benefits, including reduced adiposity. α-Klotho is a circulating protein linked to the aging processes, associated with greater longevity, and confers protective effects against cardiovascular disease, chronic kidney disease, and neurodegenerative disorders. This study investigates the association between coffee (total, caffeinated, and decaffeinated) intake and serum α-Klotho (SαKl) levels.

Protective Effects of Deer Antler Peptides on D-Galactose-Induced Brain Injury.

The aim of this study was to investigate the role and potential mechanism of deer antler peptides (DAP) in D-galactose (D-gal)-induced brain injury. In the in vivo study, C57BL/6J mice were intraperitoneally injected with 400 mg/kg D-gal and gavaged with DAP (50 and 200 mg/kg) for 5 weeks. In vitro studies, D-gal (30 μg/mL) induced senescent BV2 cells were used for further research.

Sex differences in the association between atherogenic index of plasma and α-klotho: insights from a community-based cohort.

Background: The Atherogenic Index of Plasma (AIP) is a marker of lipid imbalance and cardiovascular risk, while α-Klotho is an anti-aging protein involved in oxidative stress and inflammation regulation. Although both are linked to cardiovascular and metabolic health, the relationship between AIP and α-Klotho, particularly regarding sex differences, remains unclear.

Methods: We performed a cross-sectional analysis of a community-based cohort drawn from five consecutive cycles of the NHANES (January 2007 - December 2016).

Effects of polypeptides derived from velvet antler on D-gal induced brain aging model.

Aim: We aimed to explore the protective effects and mechanism of velvet antler polypeptides (VAP) against D-galactose (D-gal)-induced aging model in vivo and in vitro.

Methods: The mouse model of mild cognitive impairment was established by intraperitoneal injection of D-gal (400 mg/kg). After gavage of VAP (50 and 200 mg/kg) for 4 weeks, the water maze behavior test was performed.

Preparation, Characterization, and In Vitro Stability Analysis of Deer Sinew Peptide-Zinc Chelate.

Novel peptide-zinc chelates (DSPs-Zn) with a zinc content of 186.94 mg/g were synthesized from deer tendon peptides at pH 6, 60 °C, 60 min, and peptide-zinc mass ratio of 1:3. Ultraviolet-visible absorption spectroscopy (UV) and Fourier transform infrared spectroscopy (FTIR) demonstrated that the chelation sites of the deer tendon polypeptides (DSPs) with zinc ions were located at the carboxyl oxygen and amino nitrogen atoms of the peptides.

Adaptation of pilot-scale one-stage partial nitritation and anammox process to kitchen waste digestion liquid and mature landfill leachate.

This study, for the first time at pilot scale, demonstrated efficient nitrogen removal from kitchen waste digestion liquid (KWDL), mature landfill leachate (MLL), and their mixtures using a one-stage partial nitrification/anammox (PN/A) process. Nitrite-oxidizing bacteria (NOB) were effectively suppressed under low dissolved oxygen (DO) and in-situ generated high free ammonia. During KWDL treatment, the PN/A system achieved ∼ 90 % total nitrogen (TN) and 70 % chemical oxygen demand removal.

Integrating multi-omics data to optimize immunotherapy in endometrial cancer: a comprehensive study.

Immunotherapy represents a pivotal therapeutic modality in endometrial cancer (EC). Nevertheless, the efficacy of this treatment is limited to a subset of patients. The present investigation endeavors to amalgamate multi-omics data in order to elucidate the determinants impacting individual immune responsiveness and enhance the optimization of immunotherapy for EC.

Simultaneous Effects of C-N-V Bonds and Multiple Sulfides Anchoring Active V and Na Enables Honeycomb-Like Porous NaV(PO) with Non-Attenuation Characteristics for Na-Ion Batteries.

For NaV(PO) (NVP) cathode, the activity and utilization rate of Na and V is the key to affecting its electrochemical properties. However, few studies can simultaneously optimize both. Currently, a facile hydrothermal route is proposed to successfully synthesize the honeycomb-like porous NVP@CHSLS cathodes possessing anchoring effects to enhance the immobilization of active Na/V through in situ carbonation with chitosan and sodium lignosulfonate.

Predicting Sleeping Disorders After mTBI: A Role for Inflammation and Brain Network Biomarkers.

Purpose: To predict the occurrence of sleeping disorders (SD) in patients with mild traumatic brain injury (mTBI) 3 months after injury.

Methods: This study recruited a total of 232 patients with mTBI and underwent a three-month follow-up period. Demographic information, MRI images, and inflammatory factor levels were collected one month after injury and PSQI (Pittsburgh Sleep Quality Index) scores were collected three times respectively on admission, 1 month and 3 months after injury.

AKI prediction model in acute aortic dissection surgery: nomogram development and validation.

Objectives: This multicenter study developed and internally validated a biomarker-enhanced risk prediction nomogram integrating hemodynamic parameters and novel urinary biomarkers to stratify postoperative acute kidney injury (AKI) risks in patients undergoing emergency surgical repair for acute Stanford Type A aortic dissection (ATAAD).

Methods: A cohort of 1,277 patients from the China Aortic Dissection Alliance (CADA) registry was chronologically split into derivation (70%,  = 894) and validation (30%,  = 383) sets. LASSO regression with 10-fold cross-validation (λ1SE criterion) was applied to identify non-redundant predictors from 34 candidate variables (e.

Sanghuangporus vaninii polysaccharides alleviates the effects of chlorpyrifos exposure on mouse liver: role of the gut- liver axis.

Chlorpyrifos-induced oxidative stress is associated with intestinal barrier damage. Although some studies have suggested that the liver is the main target organ of chlorpyrifos, the underlying mechanisms remain incompletely understood. Initially, we determined the nontoxic effects of Sanghuangporus vaninii polysaccharide (SVP-60) by acute toxicity experiments (2000 mg/kg).

Osteocyte-derived extracellular vesicles mediate the bone-to-cartilage crosstalk and promote osteoarthritis progression.

Osteoarthritis is a common degenerative joint disease, in which mechanical overloading disrupts subchondral bone remodeling before cartilage degeneration and the osteocytes in the subchondral bone are mainly responsible for mechanosensing. However, their functional role in the early osteoarthritis is still unclear. Here we show that mechanical stress induces osteocytes in subchondral bone to secrete extracellular vesicles that accelerate cartilage metabolic dysregulation in patients with both sexes and male mice.

Ginsenoside Rg3 alleviates brain damage caused by chlorpyrifos exposure by targeting and regulating the microbial-gut-brain axis.

Introduction: The utilization of organophosphorus pesticides (OPs) has been demonstrated to exert a substantial positive influence on crop yield enhancement. However, due to the multitude of exposure routes and the persistence of these compounds, humans are routinely exposed to pesticides on a daily basis through dermal contact, inhalation, and ingestion. This has serious consequences for the health of living organisms.

Hematological and Biochemical Parameters of Subadult Captive Siberian Tigers ().

The conservation of Siberian tigers, the apex predators in the Asia-Pacific region, hinges on a thorough understanding of their physiological statuses and adaptive mechanisms. In order to fill a critical gap in our knowledge of this endangered species, we conducted comprehensive analyses of Siberian tiger whole blood and serum using modern hematology and biochemical analyzers. The blood samples were collected from subadult (2-3 years old) Siberian tigers ( = 15 for hematological analysis and = 7 for biochemical analysis).