AS2863619 boosts osteogenesis in periodontal ligament stem cells and mitigates inflammatory impairment.

Aim: Periodontal ligament stem cells (PDLSCs) represent a promising source for the regeneration of periodontal tissues. However, these cells may experience functional alterations during in vitro expansion and culture, as well as under inflammatory conditions. In this study, we investigated AS2863619 (AS), a synthetic small molecule with potential applications in immune disease research, to evaluate its impact on the osteogenic differentiation potential of PDLSCs and to elucidate its underlying mechanisms.

A novel osteopontin biomimetic polypeptide induces collagen fiber mineralization in vitro.

Collagen fibers reinforced by hydroxyapatite (HA) constitute the fundamental structures of natural bone and dentin, where non-collagenous proteins (NCPs) play a regulatory role in collagen fiber mineralization. However, the precise mechanism underlying this mineralization process remains controversial. Moreover, achieving mineralized materials with high-purity, high-mimetic, and high-efficiency, as well as elucidating the essential state of mineralized precursor, are key issues in making a breakthrough in collagen fiber mineralization.

Cyborg microbe biohybrids with metal-organic coating layers: Strategies, functionalisation and potential applications.

The integration of living microbes, specifically bacteria and fungi, with metal-organic nanocoatings has led to the recent development of cyborg microbe biohybrids, which show excellent adaptability and functionality for a wide range of potential applications in biotechnology and medicine. This review discusses the strategies, functionalisation, and applications of these biohybrids, which are categorised into two types of coatings: metal-organic frameworks (MOFs) and metal-phenolic networks (MPNs). Key advances in their synthetic approaches and pre-synthesised coatings are crucially addressed, and yet the methodology details and specific advantages are highlighted.

A multifunctional semi-interpenetrating polymer network hydrogel dressing for wound healing.

Hydrogels have exhibited significant application potential in the field of new wound dressings due to their unique physicochemical properties and biological functions. However, traditional hydrogels possess limitations regarding mechanical properties, adhesion, and the promotion of wound healing. Herein, a multifunctional polyvinyl alcohol-tannic acid/polyacrylamide-polydopamine (PVA-TA/PAM-PDA) hydrogels are developed.

Multi-active phlorotannins boost antimicrobial peptide LL-37 to promote periodontal tissue regeneration in diabetic periodontitis.

The bidirectional correlation between diabetes and periodontitis positions the latter as the most prevalent complication of the former. Rehabilitation of the periodontal tissues damaged by diabetic periodontitis presents a significant clinical challenge. The multifaceted nature of the pathogenesis of diabetic periodontitis necessitates a comprehensive approach in its treatment to mitigate its adverse effects.

LL-37 regulates odontogenic differentiation of dental pulp stem cells in an inflammatory microenvironment.

Background: Inflammation often causes irreversible damage to dental pulp tissue. Dental pulp stem cells (DPSCs), which have multidirectional differentiation ability, play critical roles in the repair and regeneration of pulp tissue. However, the presence of proinflammatory factors can affect DPSCs proliferation, differentiation, migration, and other functions.

Constructing the Enamel-Like Dentin Adhesion Interface to Achieve Durable Resin-Dentin Adhesion.

Enamel adhesion is acknowledged as durable; however, achieving long-lasting dentin adhesion remains a formidable challenge due to degradation of exposed collagen matrix after acid-etching of dentin. The idea of developing an enamel-like adhesion interface holds great promise in achieving enduring dentin adhesion. In this study, we constructed an enamel-like adhesion interface using a rapid remineralization strategy comprising an acidic primer and a rapid remineralization medium.

Nocardamine mitigates cellular dysfunction induced by oxidative stress in periodontal ligament stem cells.

Background: The role of periodontal ligament stem cells (PDLSCs) in repairing periodontal destruction is crucial, but their functions can be impaired by excessive oxidative stress (OS). Nocardamine (NOCA), a cyclic siderophore, has been shown to possess anti-cancer and anti-bacterial properties. This study aimed to investigate the protective mechanisms of NOCA against OS-induced cellular dysfunction in PDLSCs.

Extrafibrillar demineralization: Yes or no?

Objective: Extrafibrillar demineralization is considered to be an ideal solution for addressing the durability of resin-dentin bonding interfaces. However, its theoretical basis is contradictory to ionization equilibrium of hydroxyapatite dissolution. In this study, various calcium chelators were selected as dentin conditioners to explore the essence of dentin demineralization with chelators and its effect on resin-dentin adhesion.

Bottom-Up Assembling Hierarchical Enamel-Like Bulk Materials with Excellent Optical and Mechanical Properties for Tooth Restoration.

Enamel has good optical and mechanical properties because of its multiscale hierarchical structure. Biomimetic construction of enamel-like 3D bulk materials at nano-, micro-, mesh- and macro-levels is a challenge. A novel facile, cost-effective, and easy large-scale bottom-up assembly strategy to align 1D hydroxyapatite (HA) nanowires bundles to 3D hierarchical enamel structure with the nanowires bundles layer-by-layer interweaving orientation, is reported.

Clinical influencing factors of vital pulp therapy on pulpitis permanent teeth with 2 calcium silicate-based materials: A randomized clinical trial.

Background: Compared with traditional root canal therapy (RCT), vital pulp therapy (VPT) is a personalized and minimally invasive method for the treatment of pulpitis caused by dental caries. However, there are still no clear guidelines for VPT because high-quality randomized clinical trials are scarce. This prospective cohort study evaluated the clinical efficacy of VPT with the light-curable calcium silicate-based material TheraCal LC (TH) and bioceramic material iRoot BP Plus (BP) in reversible and irreversible pulpitis permanent teeth with carious exposures.

Colloidal Phenol-Amine Coating on Implants for Improved Anti-Inflammation and Osteogenesis.

Phenol-amine coatings have attracted significant attention in recent years owing to their adjustable composition and multifaceted biological functionalities. The current preparation of phenol-amine coatings, however, involves a chemical reaction within the solution or interface, resulting in lengthy preparation times and necessitating specific reaction conditions, such as alkaline environments and oxygen presence. The facile, rapid, and eco-friendly preparation of phenol-amine coatings under mild conditions continues to pose a challenge.

Er:YAG Laser Physical Etching and Ultra-High-Molecular-Weight Cross-Linked Sodium Polyacrylate Chemical Etching for a Reliable Dentin Dry Bonding.

Dentin bond interface stability is the key issue of dental adhesion in present clinical dentistry. The concept of selective extrafibrillar demineralization has opened a new way to maintain intrafibrillar minerals to prevent interface degradation. Here, using ultra-high-molecular-weight sodium polyacrylate [Carbopol (Carbo) > 40 kDa] as a calcium chelator, we challenge this concept and propose a protocol for reliable dentin dry bonding.

A programmed surface on dental implants sequentially initiates bacteriostasis and osseointegration.

Osteogenesis surrounding dental implants is initiated by a series of early physiological events, including the inflammatory response. However, the persistence of an anti-infection surface often results in compromised histocompatibility and osseointegration. Here, we presented a programmed surface containing both silver nanoparticles (AgNPs) and silver ions (Ag) with a heterogeneous structure and time-dependent functionalities.

Built-up sodium alginate/chlorhexidine multilayer coating on dental implants with initiating anti-infection and cyto-compatibility sequentially for soft-tissue sealing.

Soft-tissue sealing at transmucosal sites is very important for preventing the invasion of pathogens and maintaining the long-term stability and function of dental implants. However, the colonization of oral pathogens on the implant surface and surrounding soft tissues can disturb the early establishment of soft-tissue sealing and even induce peri-implant infection. The purpose of this study was to construct two antibacterial coatings with 5 or 10 sodium alginate/chlorhexidine bilayers on titanium surfaces using layer-by-layer self-assembly technology to promote soft-tissue sealing.

Is Etiology a Key Factor for Regenerative Endodontic Treatment Outcomes?

Introduction: This study aimed to evaluate treatment outcomes of regenerative endodontic treatment (RET) in nonvital immature permanent teeth due to developmental malformation and trauma, and to analyze the influence of etiology on the prognosis.

Methods: Fifty-five cases were included and divided into a malformation group (n = 33) and a trauma group (n = 22). Treatment outcomes were classified as healed, healing, and failure.

Oxidized Dopamine Acrylamide Primer to Achieve Durable Resin-Dentin Bonding.

The durability of the resin-dentin bonding interface is a key issue in clinical esthetic dentistry. Inspired by the extraordinary bioadhesive properties of marine mussels in a wet environment, we designed and synthetized N-2-(3,4-dihydroxylphenyl) acrylamide (DAA) according to the functional domain of mussel adhesive proteins. DAA's properties of collagen cross-linking, collagenase inhibition, inducing collagen mineralization in vitro, and as a novel prime monomer for clinical dentin adhesion use, its optimal parameters, and effect on the adhesive longevity and the bonding interface's integrity and mineralization, were evaluated in vitro and in vivo.

A homogeneous dopamine-silver nanocomposite coating: striking a balance between the antibacterial ability and cytocompatibility of dental implants.

Silver has been widely used for surface modification to prevent implant-associated infections. However, the inherent cytotoxicity of silver greatly limited the scope of its clinical applications. The construction of surfaces with both good antibacterial properties and favorable cytocompatibility still remains a challenge.

Synthesis of a novel injectable alginate impression material and impression accuracy evaluation.

Objectives: This work aimed to synthesize a novel injectable alginate impression material and evaluate its accuracy.

Methods: Certain proportions of sodium alginate, trisodium phosphate dodecahydrate, potassium fluorotitanate, diatomaceous earth, and other ingredients were dissolved in water and mixed evenly with a planetary centrifugal mixer to obtain a certain viscosity base paste. Certain proportions of calcium sulfate hemihydrate, magnesium oxide, glycerin, and polyethylene glycol (PEG) 400 were mixed evenly with a planetary centrifugal mixer to obtain the reactor paste with the same viscosity as the base paste.

Immature permanent incisors with complicated crown fractures treated with partial pulpotomy using white mineral trioxide aggregate and IRoot BP plus-a retrospective long-term study.

Background/aims: Calcium silicate cements have been widely used for pulpotomies in immature permanent teeth with complicated crown fractures due to their superior properties. However, few studies have evaluated the long-term outcomes of white mineral trioxide aggregate (WMTA) and iRoot BP Plus for partial pulpotomies. The aim of this study was to investigate the long-term clinical and radiographic outcomes of WMTA and iRoot BP Plus for partial pulpotomies in immature permanent incisors with complicated crown fractures.

Efficacy of the dual-action GA-KR12 peptide for remineralising initial enamel caries: an in vitro study.

Objective: To investigate the antibiofilm and remineralising effects of the dual-action peptide GA-KR12 on artificial enamel caries.

Materials And Methods: Enamel blocks with artificial caries were treated with sterilised deionised water as control or GA-KR12. The blocks underwent biochemical cycling with Streptococcus mutans for 3 weeks.