One-Step Labeling and Purification: Accelerating FAHFA Detection in Vegetable Oils via Chemoselective Probe-Assisted LC-MS.

Fatty acid esters of hydroxy fatty acids (FAHFAs) are emerging bioactive lipids in edible oils, but their trace-level presence and matrix complexity hinder accurate analysis. Here, we designed a novel chemoselective probe piperazine-modified silica () that specifically captures FAHFAs while removing almost totally triglycerides in a one-step labeling reaction. The probe enabled single-batch sample pretreatment within 30 min, integrating capture, purification, and signal amplification.

HBP21 Alleviates Sepsis-Induced Acute Kidney Injury by Targeting PI3K/AKT-Mediated M1 Macrophage Polarization.

Sepsis-induced acute kidney injury (S-AKI), a life-threatening complication of systemic infection, is driven by macrophage-mediated inflammatory dysregulation. This study explores the role of heat shock binding protein 21 (HBP21) in attenuating renal injury through PI3K/AKT pathway modulation, employing cellular and animal models to dissect its therapeutic mechanisms and clinical relevance. In vitro, RAW264.

Screening of proteins interacting with infectious hypodermal and hematopoietic necrosis virus in Pacific white shrimp Litopenaeus vannamei.

Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a major pathogen that severely impacts the shrimp aquaculture industry, and has lead to significant economic losses. Understanding the molecular response mechanisms of shrimp against IHHNV infection is crucial for developing effective disease control strategies. In order to deeply explore the molecular mechanism of Litopenaeus vannamei responds to IHHNV infection, this study screened the shrimp proteins interacting with IHHNV via the yeast two-hybrid system.

Machine learning combining external validation to explore the immunopathogenesis of diabetic foot ulcer and predict therapeutic drugs.

Diabetic foot ulcer (DFU) is a severe complication of diabetes, often leading to amputation due to poor wound healing and infection. The immune-related pathogenesis of DFU remains unclear, and therapeutic drugs are limited. This study aimed to explore the immune mechanisms of DFU and identify potential therapeutic drugs using machine learning and single-cell approaches.

Screening of proteins interacting with PirA and PirB from Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in Pacific white shrimp Litopenaeus vannamei.

Acute hepatopancreatic necrosis disease (AHPND) is a bacterial disease that primarily affects crustaceans such as shrimp. The causative agent is certain type of Vibrio parahaemolyticus (Vp) carrying the plasmid (pVA1) encoding the binary toxin proteins PirA and PirB. The purpose of this study is to explore the interaction mechanisms between PirA and PirB and the proteins of Litopenaeus vannamei.

Berberine Ameliorates Atrial Remodeling and Inhibits the Atrial Fibrillation of Mice via Regulating NLRP3 Inflammasome.

Increased cardiomyocyte-specific activation of the NLRP3 inflammasome contributes to the development of atrial fibrillation (AF). Berberine (BBR) exhibits numerous beneficial effects on the cardiovascular system. This study investigated how the NLRP3 inflammasome regulates susceptibility to hypertension-induced AF and the effects of BBR on this susceptibility.

The effect of the biofilm-forming F3-2 on improving lipid accumulation .

Obesity correlates with gut microbiota dysbiosis, with beneficial microbial intervention emerging as a promising therapeutic strategy for obesity-associated metabolic disorders. In previous studies, we screened F3-2 ( F3-2) with the ability to inhibit lipid accumulation, but its biofilm-forming capacity and consequent impacts on probiotic functionality remain uncharacterized. Based on this, this study characterized the biofilm formation capacity of F3-2, analyzing its structural organization along with extracellular matrix composition.

Oncogenic roles of young human de novo genes and their potential as neoantigens in cancer immunotherapy.

Young human de novo genes, recently emerging from non-coding regions, are expected to contribute to human-specific traits and diseases. However, systematic explorations of this connection have been lacking. Here, we report 37 recently originated de novo genes in humans, with their evolution and characteristics defined within an updated genomic context.

Effect of aromatherapy massage with lavender essential oil on sleep quality, pain, and mental and psychiatric disorders among breast cancer patients undergoing chemotherapy: a randomized controlled trial.

Objectives: To assess the effects of aromatherapy massage on sleep quality and mental and psychological disorders in breast cancer survivors (BCS) undergoing chemotherapy.

Methods: The study was a prospective, double-blind, randomized, placebo-controlled trial. Eligible breast cancer patients were enrolled in this study.

Different management methods for tunnel abscess in tunneled cuffed catheters with catheter-related bloodstream infections and central venous stenosis: two case reports.

Background: Tunneled cuffed catheters (TCCs) are critical vascular access tools for hemodialysis patients, but they are associated with complications such as tunnel infections (TIs) and catheter-related bloodstream infections (CRBSIs). Current guidelines recommend removing infected TCCs, but this can exacerbate central venous stenosis (CVS) and complicate subsequent catheter reinsertion. This case series proposes a staged management protocol for complex TCC tunnel abscess infections complicated by CVS.

ZFHX3 is integral to androgen/AR signaling involving protein association with AR in prostate cancer cells.

The androgen receptor (AR) signaling drives prostatic development and carcinogenesis, whereas the zinc finger homeobox 3 (ZFHX3) transcription factor modulates these processes. AR upregulates ZFHX3 transcription, but whether and how ZFHX3 plays a role in the AR signaling is unknown. RNA-seq was used to identify AR target genes that were also affected by ZFHX3 loss.

An integrated machine learning framework for developing and validating diagnostic models and drug predictions based on ulcerative colitis genes.

Ulcerative colitis (UC) is a long-lasting inflammatory bowel disease that causes inflammation in the intestines and triggers autoimmune responses. This study aims to identify immune-related biomarkers for ulcerative colitis (UC) and explore potential therapeutic targets. First, we downloaded the expression profiles of datasets GSE87466, GSE87473, and GSE92415 from the GEO database.

Medicaid Costs and Outcomes for Patients Treated in an Outpatient Telepsychiatry Clinic.

Importance: Evidence of cost savings attributable to indicated and timely care remain rare in psychiatry. Interventions to provide evidence-based psychiatric care to Medicaid patients that lower total costs of care are particularly challenging.

Objective: To investigate Medicaid costs and care outcomes associated with use of an outpatient telepsychiatry clinic.

Derivatization-Assisted Acoustic Ejection Mass Spectrometry for High-Throughput FAHFA Prescreening.

Fatty acid esters of hydroxy fatty acids (FAHFAs) are bioactive lipids with significant structural diversity and potential health benefits, playing crucial roles in metabolic regulation, inflammation, and insulin sensitivity. However, the low abundance of FAHFAs in biological samples (0.1% to 0.

Comparison of robotic, conventional, and endoscopic nipple-sparing mastectomy with immediate prosthetic breast reconstruction for breast cancer: A systematic review and meta-analysis.

In this network meta-analysis (NMA), we aimed to evaluate the relative efficacy of robotic nipple-sparing mastectomy (RNSM), conventional nipple-sparing mastectomy (CNSM), and endoscope-assisted nipple-sparing mastectomy (ENSM), each combined with immediate prosthetic breast reconstruction (IPBR), for the treatment of breast cancer. Relevant studies published up to June 15, 2024, were identified through searches of PubMed, Embase, the Cochrane Library, and Web of Science. Data extracted from these studies were analyzed using Stata 15.

Metabolic Profiling Reveals Potential Prognostic Biomarkers for SFTS: Insights into Disease Severity and Clinical Outcomes.

Severe fever with thrombocytopenia syndrome (SFTS) is a viral infection primarily found in Asia, with a case fatality rate of about 10%. Despite its increasing prevalence, the underlying pathogenic mechanisms remain poorly understood, limiting the development of effective therapeutic interventions. We employed an untargeted metabolomics approach using liquid chromatography-mass spectrometry (LC-MS) to analyze serum samples from 78 SFTS patients during the acute phase of their illness.

Exploration and practice of comprehensive clinical rotation internship for undergraduate dental students.

Background: University-based programs should aim to teach dental students about practice procedures to gain insight into day-to-day activities. This study introduces a newly established comprehensive dental professional curriculum, including an additional 9-week comprehensive rotation internship program.

Methods: All dental students from the Department of General Dentistry II under Peking University School and Hospital of Stomatology (PKUSS) who completed a specialized internship in the five research areas (endodontics, periodontics, prosthodontics, oral and maxillofacial surgery, and pediatric dentistry), including an additional 9-week comprehensive rotation, were invited to participate in the survey (total n = 253 in years 2022-2024).

An adaptive reclosing scheme for flexible DC grid based on residual voltage S-transformation.

When a fault occurs on a line, it is necessary to quickly isolate the fault to improve the safety and reliability of the transmission system. Due to the lack of effective ability to identify the nature of faults, the traditional automatic reclosing scheme is highly likely to cause the circuit breaker to reclose onto a permanent fault in practical applications. This will then cause a secondary impact on the power transmission system, seriously threatening the stable operation of the system.

The transition between M1 and M2 macrophage phenotypes is associated with the disease status following CD19 CAR-T therapy for B cell lymphoma/leukemia.

Although anti-CD19 chimeric antigen receptor (CAR-T) cells demonstrate high response rates in relapsed/refractory B-cell lymphomas, a considerable proportion of patients eventually encounter disease progression or relapse. The short-term and long-term outcomes of CAR-T treatment are intricately linked to the tumor microenvironment (TME), wherein macrophages with polarized characteristics can exhibit either anti-tumorigenic or pro-tumorigenic roles. Despite evidence implicating the crucial involvement of macrophages in CAR-T cell-treated lymphoma, their dynamic distribution and immune function related to lymphoma progression remain poorly understood.

Comprehensive analysis of CMTM family and immune infiltration in esophageal carcinoma.

Objective: Esophageal carcinoma (ESCA) is one of the most common malignant diseases and contributes to the annual burden of death worldwide. A better understanding of the underlying molecular changes is urgently required to identify early diagnostic biomarkers and effective therapeutics. The chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (CMTMs) is reported to be entangled in many human cancers.

HeuSMA: A Multigradient LC-MS Strategy for Improving Peak Identification in Untargeted Metabolomics.

Metabolomics, which involves the comprehensive analysis of small molecules within biological systems, plays a crucial role in elucidating the biochemical underpinnings of physiological processes and disease conditions. However, current coverage of the metabolome remains limited. In this study, we present a heuristic strategy for untargeted metabolomics analysis (HeuSMA) based on multiple chromatographic gradients to enhance the metabolome coverage in untargeted metabolomics.

Unraveling the Metabolic and Microbiome Signatures in Fecal Samples of Pregnant Women with Prenatal Depression.

: Prenatal depression (PND) poses a significant threat to the health of both the mother and the developing fetus. Despite its increasing prevalence, the pathophysiology of PND is not yet fully elucidated. : In this study, we aimed to investigate the fecal metabolites and gut microbiota in PND patients compared to healthy controls and to explore potential correlations between these factors.

Rational design, optimization, and biological evaluation of novel pyrrolo-pyridone derivatives as potent and orally active Cbl-b inhibitors.

Casitas B-lineage lymphoma proto-oncogene-b (Cbl-b), a member of the Cbl family of RING finger E3 ubiquitin ligases, plays a critical role in negatively regulating T-cell, natural killer (NK) cell, and B-cell activation. Inhibiting Cbl-b has emerged as a promising immuno-oncology strategy to enhance immune cell function. Here, we describe the rational design and optimization of pyrrolo-pyridone derivatives as potent Cbl-b inhibitors.