Radiographic parameters and feasibility analysis of dual sacral-2 alar-iliac screw fixation using three-dimensional digital technology.

Objective: The purpose of this study was to evaluate the feasibility and the trajectories of dual sacral-2 alar-iliac (S2AI) screw fixation with three-dimensional digital technology simulation analysis.

Methods: The pelvic computed tomography scan data of 60 (30 men and 30 women, age: 25-86 years) individuals were selected and reconstructed. The trajectories of dual S2AI screws were plotted using three-dimensional reconstruction software.

Predicting Stroke-Associated Pneumonia in Acute Ischemic Stroke: A Machine Learning Model Development and Validation Study with CBC-Derived Inflammatory Indices.

Purpose: Stroke-associated pneumonia (SAP), a critical complication of ischemic stroke, significantly worsens outcomes. Our aim was to identify SAP risk factors and develop a machine learning (ML) model for early risk stratification.

Methods: This retrospective study analyzed 574 ischemic stroke patients, divided into training (75%) and testing (25%) sets.

Molecular insights and clinical implications of DNA methylation in sepsis-associated acute kidney injury: a narrative review.

Sepsis-induced acute kidney injury (S-AKI) is a life-threatening complication of sepsis, marked by dysregulated inflammation, metabolic derangements, and immune dysfunction, driving high mortality. Its multifactorial pathogenesis increasingly implicates DNA methylation-a core epigenetic mechanism-as a critical disease modulator. This review synthesizes current knowledge of DNA methylation in S-AKI, covering molecular mechanisms, cellular dysfunction, and translational potential.

Optimal control analysis for the transmission of Nipah infection with imperfect vaccination.

This paper presents an innovative mathematical model for assessing the dynamics and optimal control of Nipah virus (NiV) with imperfect vaccination. The model formulation considers transmissions through contaminated food and human-to-human contacts. It also incorporates the potential virus transmission through contact with a deceased body infected with NiV.

Development and validation of a prediction model for in-hospital mortality in patients with sepsis.

Background: Sepsis, a life-threatening condition marked by organ dysfunction due to a dysregulated host response to infection, involves complex physiological and biochemical abnormalities.

Aim: To develop a multivariate model to predict 4-, 6-, and 8-week mortality risks in intensive care units (ICUs).

Study Design: A retrospective cohort of 2389 sepsis patients was analysed using data captured by a clinical decision support system.

Simultaneous variable selection and estimation for a partially linear Cox model.

We consider simultaneous variable selection and estimation for a deep neural network-based partially linear Cox model and propose a novel penalized approach. In particular, a two-step iterative algorithm is developed with the use of the minimum information criterion to ensure sparse estimation. The proposed method circumvents the curse of dimensionality while facilitating the interpretability of linear covariate effects on survival, and the algorithm greatly reduces the computational burden by avoiding the need to select the optimal tuning parameters that is usually required by many other popular penalties.

Cholesteric liquid crystal based polymer anti-counterfeit lamination films with broadband hyper-reflective properties and effective shielding against infrared laser beams.

We innovatively propose broadband hyper-reflective bilayer polymer dispersed cholesteric liquid crystal (PDCLC) films with difficult diffusion between the upper and lower films. Single-layer PDCLC films with broadband reflective properties were prepared using light-induced molecular diffusion and a polymer network-anchored pitch. Then, bilayer PDCLC films with broadband hyper-reflective properties were prepared by filling liquid crystal mixtures of opposite rotations into a specially designed bilayer liquid crystal cassette, and further broadened the reflective broadband by constructing a bilayer liquid crystal system.

The protein Mhp274 elicits mucosal and systemic immune responses in mice.

Background: is the etiological agent of mycoplasmal pneumonia of swine (MPS). Commercial vaccines provide partial protection and do not prevent the colonization and transmission of . The bottleneck in the development of more effective vaccines for MPS is the stimulation of effective immune responses in the host.

Conditional modeling of recurrent event data with terminal event.

Recurrent event data with a terminal event arise in follow-up studies. The current literature has primarily focused on the effect of covariates on the recurrent event process using marginal estimating equation approaches or joint modeling approaches via frailties. In this article, we propose a conditional model for recurrent event data with a terminal event, which provides an intuitive interpretation of the effect of the terminal event: at an early time, the rate of recurrent events is nearly independent of the terminal event, but the dependence gets stronger as time goes close to the terminal event time.

Advanced stability analysis of a fractional delay differential system with stochastic phenomena using spectral collocation method.

In recent years, there has been a growing interest in incorporating fractional calculus into stochastic delay systems due to its ability to model complex phenomena with uncertainties and memory effects. The fractional stochastic delay differential equations are conventional in modeling such complex dynamical systems around various applied fields. The present study addresses a novel spectral approach to demonstrate the stability behavior and numerical solution of the systems characterized by stochasticity along with fractional derivatives and time delay.

Serovar and sequence type distribution and phenotypic and genotypic antimicrobial resistance of originating from pet animals in Chongqing, China.

A total of 334 isolates were recovered from 6,223 pet rectal samples collected at 50 pet clinics, 42 pet shops, 7 residential areas, and 4 plazas. Forty serovars were identified that included all strains except for one isolate that did not cluster via self-agglutination, with Typhimurium monophasic variant, Kentucky, Enteritidis, Pomona, and Give being the predominant serovars. Fifty-one sequence types were identified among the isolates, and ST198, ST11, ST19, ST451, ST34, and ST155 were the most common.

Finite Element Analysis of Six Internal Fixations in the Treatment of Pauwels Type III Femoral Neck Fracture.

Objective: The current investigation sought to utilize finite element analysis to replicate the biomechanical effects of different fixation methods, with the objective of establishing a theoretical framework for the optimal choice of modalities in managing Pauwels type III femoral neck fractures.

Methods: The Pauwels type III fracture configuration, characterized by angles of 70°, was simulated in conjunction with six distinct internal fixation methods, including cannulated compression screw (CCS), dynamic hip screw (DHS), DHS with de-rotational screw (DS), CCS with medial buttress plate (MBP), proximal femoral nail anti-rotation (PFNA), and femoral neck system (FNS). These models were developed and refined using Geomagic and SolidWorks software.

Interferon-regulatory factor-1 boosts bevacizumab cardiotoxicity by the vascular endothelial growth factor A/14-3-3γ axis.

Aim: Myocardial injury is a significant cause of death. This study investigated the role and underlying mechanism of interferon-regulatory factor-1 (IRF1) in bevacizumab (BVZ)-induced cardiomyocyte injury.

Methods And Results: HL-1 cells and C57BL/6 mice receiving BVZ treatment were used to establish in vitro and in vivo models of myocardial injury.

Unlocking the potential of resuscitation-promoting factor for enhancing anaerobic microbial dechlorination of polychlorinated biphenyls.

Microbial dechlorination of polychlorinated biphenyls (PCBs) is limited by the slow growth rate and low activity of dechlorinators. Resuscitation promoting factor (Rpf) of Micrococcus luteus, has been demonstrated to accelerate the enrichment of highly active PCB-dechlorinating cultures. However, it remains unclear whether the addition of Rpf can further improve the dechlorination performance of anaerobic dechlorination cultures.

-positive serovars Agona and Derby are prevalent among food-producing animals in Chongqing, China.

is one of the most important foodborne zoonotic pathogens, causing global morbidity and mortality in both humans and animals. Due to the extensive use of antimicrobials in food-producing animals, the antimicrobial resistance of has attracted increasing attention globally. There have been many reports concerning the antimicrobial resistance of from food-producing animals, meats and the environment.

Altered intestinal microbiome and metabolome correspond to the clinical outcome of sepsis.

Background: The gut microbiome plays a pivotal role in the progression of sepsis. However, the specific mechanism of gut microbiota and its metabolites involved in the process of sepsis remains elusive, which limits its translational application.

Method: In this study, we used a combination of the microbiome and untargeted metabolomics to analyze stool samples from patients with sepsis enrolled at admission, then microbiota, metabolites, and potential signaling pathways that might play important roles in disease outcome were screened out.

Resuscitation-Promoting Factor Accelerates Enrichment of Highly Active Tetrachloroethene/Polychlorinated Biphenyl-Dechlorinating Cultures.

The anaerobic bioremediation of polychlorinated biphenyls (PCBs) is largely impeded by difficulties in massively enriching PCB dechlorinators in short periods of time. Tetrachloroethene (PCE) is often utilized as an alternative electron acceptor to preenrich PCB-dechlorinating bacteria. In this study, resuscitation promoting factor (Rpf) was used as an additive to enhance the enrichment of the microbial communities involved in PCE/PCBs dechlorination.

High-dose vitamin C on sepsis: Protocol of a prospective, multi-centered, double-blinded, randomized, and placebo-controlled superiority study.

Background: Sepsis is an inflammatory syndrome with life-threatening organ dysfunction and high mortality. In the recent 10 years, high-dose intravenous injection of vitamin C, the first-line antioxidant of humans, has received highlighted attention in the field of critical care. The study aims to examine the efficacy and safety of high-dose intravenous injection of vitamin C in the treatment of sepsis.

Multiple cannulated screw fixation of femoral neck fractures with comminution in young- and middle-aged patients.

Objective: To investigate the distribution and influence of comminution in femoral neck fracture (FNF) patients after cannulated screw fixation (CSF).

Methods: From January 2019 to June 2020, a total of 473 patients aged 23-65 years with FNF treated by CSF were included in the present study. Based on location of the cortical comminution, FNF patients were assigned to two groups: the comminution group (anterior comminution, posterior comminution, superior comminution, inferior comminution, multiple comminutions) or the without comminution group.

Optimization of bacterial cytokine protein production by response surface methodology for environmental bioremediation.

In natural and engineered systems, most microorganisms would enter a state of dormancy termed as "viable but non-culturable" (VBNC) state when they are exposed to unpredictable environmental stress. One of the major advances in resuscitating from such a state is the discovery of a kind of bacterial cytokine protein called resuscitation-promoting factor (Rpf), which is secreted from . In this study, the optimization of Rpf production was investigated by the response surface methodology (RSM).

Oxidative dehalogenation and mineralization of polychlorinated biphenyls by a resuscitated strain Streptococcus sp. SPC0.

Most functional microorganisms cannot be cultivated due to entering a viable but non-culturable (VBNC) state, which limits the characterization and application of polychlorinated biphenyl (PCB)-degrading strains. Resuscitating VBNC bacteria could provide huge candidates for obtaining high-efficient PCB degraders. However, limited studies have focused on the ability of resuscitated strains for PCBs degradation.

Sex Disparities of Genomic Determinants in Response to Immune Checkpoint Inhibitors in Melanoma.

Background: Despite the acknowledged sex-related differences in immune response and immune checkpoint inhibitor (ICI) efficacy, little is known about the sex disparities in melanoma of novel genomic determinants for ICI therapies.

Methods: Pretreatment genomic profiles and clinical characteristics of 631 melanoma patients treated with ICIs (i.e.

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish ().

This study aimed to determine the effect of the growth stage of on their intestinal microbiota. Intestinal samples of five different growth stages of (first instar, second instar, third instar, juvenile, and adult) from laboratory culture were analyzed through the Illumina MiSeq high-throughput sequencing platform to determine the intestinal microbiome of crayfish. The alpha diversity decreased along with the growth of the crayfish, with the relative abundance of the microbiota changing among stages; crayfish at closer development stages had a more comparable intestinal microbiota composition.

Viable but Nonculturable State of Yeast sp. Strain LN1 Induced by High Phenol Concentrations.

Microbial degradation plays an important role in environmental remediation. However, most microorganisms' pollutant-degrading capabilities are weakened due to their entry into a viable but nonculturable (VBNC) state. Although there is some evidence for the VBNC state of pollutant-degrading bacteria, limited studies have been conducted to investigate the VBNC state of pollutant degraders among fungi.