Resistomic features and novel genetic element identified in hospital wastewater with short- and long-read metagenomics.

The global spread of antimicrobial resistance (AMR) poses a serious threat to public health, with hospital wastewater treatment plants (WWTPs) recognized as a key hotspot for resistant pathogens and antibiotic resistance genes (ARGs). This study employed advanced hybrid sequencing platforms to provide a comprehensive resistomic analysis of a Qingdao WWTP in China, revealing previously uncovered AMR transmission risks. We identified 175 ARG subtypes conferring resistance to 38 antimicrobials, including the last-resort antibiotics, highlighting the extensive and concerning resistance reservoir within this environment.

A network analysis of gender differences in unresolved attachment, post-traumatic stress following COVID-19, psychiatric comorbidity, and contamination fear in adolescents: Implications for psychotherapy.

Objectives: This study used a network approach to examine gender differences in adolescents regarding unresolved attachment styles, COVID-19 PTSD symptoms, comorbid psychiatric symptoms, and contamination fear. The rationale for the study stems from the suggestion that parents' relationships with adolescents (i.e.

Polarity-switching self-calibrated MIP-PEC sensor based on core-shell S-TiO/CDs/PCN-224 for accurate detection of tetracycline.

Polarity switching photoelectrochemical (PEC) sensing effectively addresses the issue of false positive or negative results that arise from single signal on or off in PEC systems. In this study, a novel core-shell ternary composite material of S-TiO/CDs/PCN-224 was constructed by growing PCN-224 on the surface of spherical titanium dioxide (S-TiO) while simultaneously confining carbon quantum dots (CDs). This ternary composite can produce cathodic and anodic PEC signals under visible (420-800 nm) and ultraviolet (UV)-Vis (320-800 nm) excitation, respectively.

Healthy Lifestyle Matters: A Network Analysis of Urban Chinese Pre-School Children's Adiposity, Sleep Health, Mental Health, Child Functioning and Health Behaviours.

Background: In the Chinese context, a greater emphasis is placed on academic skills rather than healthy lifestyles among pre-school children. To promote well-being as a whole, a comprehensive interpretation of multivariant relationships between health, functioning and well-being is necessary for children in this age group.

Methods: The current study adopted a network analysis among 422 urban Chinese pre-school children (57.

Mn doped Fe-gallate acid MOFs loaded on the surface of biomass cotton fibers: Fenton-like degradation of tetracycline.

In this study, the novel composite material was synthesized by loading Mn-doped Fe-based gallic acid metal-organic frameworks (MOFs) onto biomass cotton fibers (Mn/Fe-GA-MOFs@CF). Mn doping improved the catalytic efficiency of Fenton-like reactions. Moreover, the MOFs loading onto cotton fibers enhanced the recoverability and practical usability.

Unraveling ancient wisdom: The molecular mechanism of Gujin Luyan Xuming decoction in combating cerebral ischemia-reperfusion injury.

Background: Cerebral infarction significantly impairs the quality of life of affected patients, emphasizing the need for research into novel targeted therapeutic agents. For nearly 2000 years, Gujin Luyan Xuming decoction (GJLYXMD) has been a classical treatment for this condition; however, the precise molecular mechanism remains unclear.

Purpose: This study aimed to examine the mechanism of GJLYXMD in middle cerebral artery occlusion/reperfusion (MCAO/R) rats via the integration of multi-omics approaches, network pharmacology, and molecular dynamics simulation.

Pressure-Modulated Quantum Melting of Spin Order in the Kondo Lattice MnTaS.

Controlling and understanding the quantum phase transitions (QPTs) of the magnetically ordered phases of strongly correlated systems, such as heavy-Fermion materials, cuprate superconductors, is essential for uncovering the new physical phenomena in condensed matter physics. This study reports the observation of pressure-modulated magnetic QPTs (MQPTs) and heavy-Fermion-like behavior in a manually constructed Kondo lattice─MnTaS. At low temperatures, pressure application induces quantum melting of magnetic order within the helical magnetic configuration of MnTaS.

The relationship between ageism, loneliness, and anxiety in widowed older adults: cognitive function as a moderator in the mediated model.

Objective: As the global population ages and traditional family support structures decline, mental health issues-particularly anxiety-among widowed older adults have become increasingly prevalent. Studies have shown a positive correlation between ageism and anxiety disorders in older bereaved individuals, but the underlying mechanism by which ageism affects anxiety in this population has not yet been clarified. This study aimed to examine whether loneliness mediates the relationship between age discrimination and anxiety disorders, and whether cognitive functioning moderates this mediation.

Somatostatin receptor 2 targeting peptide modifications for peptide-drug conjugate treatment of small cell lung cancer.

Peptide-drug conjugate (PDC) represents a special therapeutic strategy to enhance drug delivery by targeting tumor cell receptors while minimizing off-target effects. Comparing the antibody-drug conjugate (ADC), the targeting peptide constitutes the pivotal component of PDC, especially with easy optimization of peptides to promote their in vivo stability, and with the agonist stimulated GPCR internalization to facilitate drug distribution into tumor cell plasma. Herein, we have optimized a highly stable peptide molecule LanTC targeting somatostatin receptor 2 (SSTR2), through amino acid substitution and disulfide bond modification from an FDA proved peptide drug Lanreotide.

Photoplethysmography parameters in monitoring nociception during general anesthesia.

Background: Validated monitoring methods for evaluating the balance of nociception and anti-nociception (BNAN) are needed in general anesthesia. This study assessed six photoplethysmography (PPG) parameters, computed from finger photoplethysmographic waveforms in patients undergoing gynecological surgery under general anesthesia.

Methods: A total of 20 participants were included, each undergoing general anesthesia with propofol and remifentanil.

Cognitive function moderates the mediation of resilience in the relationship between perceived receipt of filial piety and loneliness among community-dwelling older adults with multiple long-term conditions.

Given the rapidly aging population and the changes in traditional family bonds, new challenges arise for filial piety in the loneliness among older adults with multiple long-term conditions (MLTCs). This study aimed to examine the PRFP-loneliness relationship, explore whether resilience mediates the association between PRFP and loneliness, and investigate whether cognitive function moderates the indirect or direct effects of the mediation model among Chinese older adults with MLTCs. A total of 635 older adults with MLTCs were recruited in Shenyang City, China.

Post-traumatic stress disorder and risk of systemic lupus erythematosus: Meta-analysis and Mendelian randomization study.

Objective: We conducted a meta-analysis and Mendelian randomization study to analyze the association and causal relationship of genetic prediction between post-traumatic stress disorder and the risk of systemic lupus erythematosus.

Methods: A meta-analysis was conducted using hazard ratio, relative risk, odds ratio, and 95 % confidence intervals as effect measures. Summary data from genome-wide association studies on post-traumatic stress disorder and systemic lupus erythematosus were utilized to assess the causal relationship of genetic prediction between post-traumatic stress disorder and systemic lupus erythematosus using Mendelian randomization.

Pulling nutrients from Mo-polluted soil by arbuscular mycorrhizal fungi extraradical mycelia is quenching thirsty with poison.

As an extension of plant root system, arbuscular mycorrhizal fungi (AMF) extraradical mycelium (ERM) can break the limitation of rhizosphere and play an important role in plant nutrient acquisition. However, it remains unclear whether ERM is smart enough to pick out nutrients while avoiding poison, or is unable to pick out nutrients and have to absorb poisons together. Therefore, the present study employed a compartment device to separate the mycelia from roots, aiming to explore the nutrient absorption pathways of mycelia in molybdenum (Mo) pollution soil after inoculation with AMF in maize and vetch plants.

Mechanisms of tigecycline resistance in Gram-negative bacteria: A narrative review.

Tigecycline serves as a critical "final-resort" antibiotic for treating bacterial infections caused by multidrug-resistant bacteria for which treatment options are severely limited. The increasing prevalence of tigecycline resistance, particularly among Gram-negative bacteria, is a major concern. Various mechanisms have been identified as contributors to tigecycline resistance, including upregulation of nonspecific Resistance Nodulation Division (RND) efflux pumps due to mutations in transcriptional regulators, enzymatic modification of tigecycline by monooxygenase enzymes, and mutations affecting tigecycline binding sites.

Synergistic effect of 2D covalent organic frameworks confined 0D carbon quantum dots film: Toward molecularly imprinted cathodic photoelectrochemical platform for detection of tetracycline.

The development of high photoactive cathode materials combined with the formation of a stable interface are considered important factors for the selective and sensitive photoelectrochemical (PEC) detection of tetracycline (TC). Along these lines, in this work, a novel type II heterostructure composed of two-dimensional (2D) covalent organic frameworks confined to zero-dimensional (0D) carbon quantum dots (CDs/COFs) film was successfully synthesized using the rapid in-situ polymerization method at room temperature. The PEC signal of CDs/COFs was significantly amplified by improving the light absorption and electron transfer capabilities.

CHK1 controls zygote pronuclear envelope breakdown by regulating F-actin through interacting with MICAL3.

CHK1 mutations could cause human zygote arrest at the pronuclei stage, a phenomenon that is not well understood at the molecular level. In this study, we conducted experiments where pre-pronuclei from zygotes with CHK1 mutation were transferred into the cytoplasm of normal enucleated fertilized eggs. This approach rescued the zygote arrest caused by the mutation, resulting in the production of a high-quality blastocyst.

Exposure to polystyrene microplastics during lactational period alters immune status in both male mice and their offspring.

The widespread use of microplastics and their harmful effects on the environment have emerged as serious concerns. However, the effect of microplastics on the immune system of mammals, particularly their offspring, has received little attention. In this study, polystyrene microplastics (PS-MPs) were orally administered to male mice during lactation.

Lactating exposure to microplastics at the dose of infants ingested during artificial feeding induced reproductive toxicity in female mice and their offspring.

Microplastics (MPs) pollution poses a global environmental challenge with significant concerns regarding its potential impact on human health. Toxicological investigations have revealed multi-system impairments caused by MPs in various organisms. However, the specific reproductive hazards in human contexts remain elusive, and understanding the transgenerational reproductive toxicity of MPs remains limited.

Effect of exercise on postoperative recovery of patients with non-small cell lung cancer: a systematic review and meta-analysis.

Unlabelled: Patients with non-small cell lung cancer (NSCLC) in the postoperative recovery period often experience reduced exercise capacity and impaired lung function, which affects their overall quality of life. This paper investigated the effect of exercise interventions on exercise capacity, lung function, quality of life, and symptoms in these patients.

Methods: We performed a literature search across Cochrane, Embase, PubMed, Web of Science, and EBSCO databases were comprehensively searched for randomized controlled trials (RCTs) from inception to September 2023, all English RCTs were eligible if they assessed the effects of exercise interventions on postoperative NSCLC patients.

Geniposide ameliorates psoriatic skin inflammation by inhibiting the TLR4/MyD88/NF-κB p65 signaling pathway and MMP9.

Psoriasis is an incurable immune-mediated disease affecting the skin or the joints. There are continuing studies on drugs for psoriasis prevention and treatment. This research found that Geniposide (GE) significantly thinned IMQ mice's skin lesions, reduced the scales, and lowered the presence of inflammatory cells in the pathology in a dose-dependent manner.

Abnormalities of retinal function in type 2 diabetes mellitus patients without clinical diabetic retinopathy detected by multifocal electroretinogram.

Objective: To study the changes of retinal function in type 2 diabetes mellitus(DM) patients without apparently diabetic retinopathy via multifocal electroretinogram.

Methods: Thirty-six type 2 DM patients (72 eyes) without visible diabetic retinopathy were selected as the experimental group, and thirty-five healthy subjects (70 eyes) were selected as the control group. All subjects were underwent multifocal electroretinogram (mf- ERG).

High-fat and high-sucrose diet impairs female reproduction by altering ovarian transcriptomic and metabolic signatures.

Background: Excessive energy intake in modern society has led to an epidemic surge in metabolic diseases, such as obesity and type 2 diabetes, posing profound threats to women's reproductive health. However, the precise impact and underlying pathogenesis of energy excess on female reproduction remain unclear.

Methods: We established an obese and hyperglycemic female mouse model induced by a high-fat and high-sucrose (HFHS) diet, then reproductive phenotypes of these mice were evaluated by examing sexual hormones, estrous cycles, and ovarian morphologies.

Metal-organic-framework-confined quantum dots enhance photocurrent signals: A molecularly imprinted photoelectrochemical cathodic sensor for rapid and sensitive tetracycline detection.

Background: Tetracycline (TC), a cost-effective broad-spectrum antibacterial drug, has been excessively utilized in the livestock and poultry industry, leading to a serious overabundance of TC in livestock wastewater. However, conventional analytical methods such as liquid chromatography and gas chromatography face challenges in achieving sensitive detection of trace amounts of TC in complex substrates. Therefore, it is imperative to develop a highly sensitive and anti-interference analytical method for the detection of tetracycline in livestock wastewater.

Preclinical and early clinical studies of a novel compound SYHA1813 that efficiently crosses the blood-brain barrier and exhibits potent activity against glioblastoma.

Glioblastoma (GBM) is the most common and aggressive malignant brain tumor in adults and is poorly controlled. Previous studies have shown that both macrophages and angiogenesis play significant roles in GBM progression, and co-targeting of CSF1R and VEGFR is likely to be an effective strategy for GBM treatment. Therefore, this study developed a novel and selective inhibitor of CSF1R and VEGFR, SYHA1813, possessing potent antitumor activity against GBM.