Identification and characterization of the first G8P[1] sheep rotavirus strain in China: Genetic similarity to human rotavirus and zoonotic potential.

Rotavirus (RV) is a major cause of gastroenteritis in both humans and many mammals, including livestock. However, information regarding RV in sheep remains limited, particularly in China. This study reports the first isolation and characterization of the G8-P[1]-I2-R2-C2-M2-A11-N2-T6-E2-H3 type sheep RV strain (GS13) in China.

HIF-1α-mediated inhibition of the sFlt-1/sENG/TNF-α pathway promotes angiogenesis to ameliorate pre-eclampsia.

Pre-eclampsia (PE) is a common pregnancy complication, closely associated with endothelial dysfunction and inhibition of angiogenesis. This study aims to explore the pathological mechanisms causing endothelial dysfunction and suppressed angiogenesis in PE, with the aim of identifying potential drug targets. Human umbilical vein endothelial cells (HUVECs) were exposed to angiotensin II (Ang-II) to mimic PE-related endothelial dysfunction.

Research Progress on the Gc Proteins of Akabane Virus.

The Akabane virus (AKAV) is a significant member of the Orthobunyavirus genus, with its envelope glycoprotein Gc, focusing on its molecular structural features, immunoregulatory mechanisms, and application value in pathogen diagnosis and vaccine design. As a key structural protein of AKAV, Gc mediates virus adsorption and neutralizing antibody recognition through the N-terminal highly variable region (HVR), while the C-terminal conserved region (CR) dominates the membrane fusion process, and its glycosylation modification has a significant regulatory effect on protein function. In clinical diagnostics, serological assays based on Gc proteins (e.

Ultrasound-assisted alkaline pH-shifting fabrication of rice bran protein-tea saponin complexes: Mechanistic insights into the role of saponin concentration on structural characteristics and emulsion stability.

To enhance the emulsion stability of rice bran protein (RBP), complexes of RBP and tea saponin (TS) were prepared via ultrasound-assisted alkaline pH-shifting, and effects of TS concentration (0-20 mg/mL) on the structural characteristics and emulsion stability of RBP-TS complex were analyzed. As TS concentration increased, the free amino group content, disulfide bond content, and surface hydrophobicity of complexes decreased, while the absolute ζ-potential of complexes enhanced from 28.84 to 33.

Comprehensive bioinformatics analysis of EXOSC family genes in head and neck squamous cell carcinoma.

Head and neck squamous cell carcinoma (HNSC) is a highly aggressive malignancy with poor prognosis, necessitating the identification of novel biomarkers for improved diagnosis and treatment. The exosome complex (EXOSC) family plays a crucial role in RNA metabolism, but its significance in HNSC remains poorly understood. We performed a comprehensive multi-omics analysis integrating data from TCGA, GEO, CPTAC, and the Human Protein Atlas to investigate the expression, prognostic value, and immune relevance of EXOSC genes in HNSC.

Multiple synchronous primary malignant neoplasms of renal, ureter and urinary bladder: a case report.

Synchronous multiple primary malignant neoplasms (MPMNs) involving distinct histopathological entities within the upper urinary tract represent a rare clinical phenomenon. We present a novel case of a 57-year-old male presenting with asymptomatic gross hematuria, subsequently diagnosed with three concurrent malignancies: papillary renal cell carcinoma(T1bN0M0), verrucous carcinoma of the renal pelvis (T1N0M0), and low-grade invasive urothelial carcinoma of the ureter (T2N0M0). Comprehensive imaging evaluation revealed a 5.

A visible-light-promoted perfluoroalkylation/cyclization cascade towards perfluoroalkyl-substituted iminoisobenzofurans using the EDA complex.

Perfluoroalkyl-functionalized heterocycles exhibit profoundly modified bioactivities, motivating the development of efficient methods for precise perfluoroalkyl group installation. A new metal-free photoinduced radical-initiated cascade reaction was developed for the rapid synthesis of perfluoroalkyl-substituted iminoisobenzofurans by reacting various perfluoroalkyl iodides (R-I) under mild reaction conditions. In this reaction system, the formation of electron donor-acceptor (EDA) complexes is pivotal for the visible-light promoted transformation in the absence of additional photocatalytic species.

Serum metabolomics in pulmonary sarcoidosis: metabolic signatures across prognoses.

Background: Sarcoidosis is a systemic inflammatory disease, primarily affecting the lungs, with a prognosis that varies widely among patients. While some patients recover spontaneously after diagnosis, others experience disease progression. Currently, the metabolomic profile associated with pulmonary sarcoidosis and its different clinical outcomes remains poorly understood.

The Relationship Between Childhood Abuse and Bullying Victimization: A Three-Level Meta-Analysis.

The link between childhood abuse and the likelihood of experiencing bullying victimization is well-established; yet, empirical research has presented divergent findings on this connection, with the moderating influences remaining obscure. Utilizing a three-tiered meta-analytic framework, this investigation aims to provide a thorough synthesis and quantitative assessment of the relationship between childhood abuse and bullying victimization. The analysis encompassed 35 studies, comprising 84 effect sizes across a participant pool of 72,691 individuals.

Efficacy of an optimal vaccination strategy for H120 and NNA vaccines against the novel HX strain of the IBV GVI-1 genotype.

The infectious bronchitis virus (IBV) causes significant economic losses to the global poultry industry. Recently, there has been a rapid spread of the GVI-1 lineage of IBV in Asia, particularly in China. However, to date there have been few studies that have assessed the immune protection efficacy of commonly used IB vaccines against the GVI-1 lineage strains.

Pyroptosis, a double-edged sword during pathogen infection: a review.

Pyroptosis, a distinctive form of programmed cell death (PCD) characterized by its inflammatory nature, is triggered by the activation of pore-forming proteins known as gasdermins (GSDMs). This process is marked by progressive expansion of a pore within the cell, ultimately leading to cellular membrane disruption and the substantial release of intracellular contents. Pyroptosis plays a pivotal role in the eradication of intracellular pathogen replication niches and in the modulation of the immune system through the release of danger signals.

Synthesis of quinazoline derivatives and their in vitro inhibition activity against MDA-MB-231 cells and A549 cells.

A series of novel 4-aniline quinazoline derivatives (Y1-Y26) were synthesised from 2-amino-6-nitrobenzoic acid based on the quinazoline parent nucleus via trifluoroacetylation, ring-closing and chlorination; The CCK-8 method was used to assess the in vitro inhibitory activities of the resulting compounds against two distinct cell lines: breast cancer cells (MDA-MB-231) and non-small cell lung cancer (A549). The results demonstrated that most of the compounds exhibited in vitro proliferation inhibitory activity against both MDA-MB-231 and A549 cells. Among these, compound Y22 exhibited the strongest inhibitory effect on MDA-MB-231 cells (IC = 4.

Isolation and identification of an AKAV strain in dairy cattle in China.

Akabane disease is an arthropod-borne disease caused by Akabane virus (AKAV), which is characterized by abortion, premature birth, stillbirth, congenital arthrosis, and hydrocephalic anencephalic syndrome in pregnant cattle and sheep. The occurrence of AKAV was proved by RT-PCR amplification based on AKAV S fragment, virus isolation, cells inoculation, cytopathy, transmission electron microscopy, and gene sequencing. The PCR amplicon was approximately 850 bp and was sequenced, and molecular identification of AKAV was conducted through phylogenetic analysis of S gene sequence.

Recombination and Genetic Diversity Analysis of Porcine Reproductive and Respiratory Syndrome 1 Nonstructural Protein 2 Genes in China.

Background: Porcine reproductive and respiratory syndrome (PRRS) has been present in China for about 30 years, and because of the high mutability of PRRSV, it causes huge economic losses to pig enterprises every year. PRRSV-2 is widely prevalent in China, and the detection rate of PRRSV-1 is also on the rise. Nonstructural protein 2 (NSP2) is a highly variable protein with multiple biological functions, such as PRRSV replication, which plays an important role in understanding PRRSV variation and epidemic alerts.

Genetic variation in NSP4 of type 1 porcine reproductive and respiratory syndrome virus in China.

Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is characterized by a high mutation rate. Although research has largely focused on PRRSV-2, the detection rate of PRRSV-1 in China is increasing. To investigate the prevalence and genetic variation of NSP4, a key gene in the regulation of host immunity, in PRRSV-1 in China, publicly available whole-genome sequence data and NSP4 sequences were obtained for analyses of nucleotide and amino acid homology, substitutions, and phylogenetic relationships.

A Rare Case of Small Bowel Ulceration Induced by COVID-19.

Background: COVID-19 can affect multiple organ systems beyond the respiratory tract, including the gastrointestinal tract, where gastrointestinal symptoms include nausea, vomiting, diarrhea, abdominal pain, and even serious manifestations such as ulcers, perforation, or gastrointestinal bleeding.

Case Presentation: We report a case of a 45-year-old male patient with small bowel ulcers caused by chronic COVID-19 infection. Initially presenting with fever and transient unconsciousness, he developed ischemic necrosis and required a mid-thigh amputation.

Optimization of Short-Range Order in Amorphous AlO Nanosheets for Enhanced Methane Oxidation.

Heterogeneous catalysts often undergo dynamic evolution during catalysis, forming true active sites. Amorphous materials, due to their inherent structural flexibility, are particularly prone to evolution and self-adaptation under catalytic conditions. Herein, we demonstrate that the short-range order of an Al-O polyhedron in amorphous aluminum oxide nanosheets undergoes a transformation from a mixed AlO, AlO, and AlO configuration to a randomly connected AlO structure during both hydrothermal treatment and direct methane oxidation, confirmed by time-series Al solid-state NMR spectroscopy.

Carvedilol sensitizes chemotherapy by targeting STING to boost anti-tumor immunity.

The stimulator of interferon genes (STING)-mediated type I interferon (IFN) response is critical for mounting anti-tumor immunity and sensitizing chemotherapy by remodeling the tumor immune microenvironment. However, no clinically available drugs have been applied for STING activation. Based on high-throughput screening of small-molecule microarrays, we found that carvedilol, an adrenergic receptor blocker used to treat essential hypertension and symptomatic heart failure, is a STING activator.

Effect of Wheat Varieties and Cultivation Environments on Grain Endophytes, Microbial Communities, and Quality of Medium-High Temperature Daqu in Chinese Baijiu.

Wheat grain serves as the primary raw material for producing medium-high temperature (MT)-Daqu, a fermentation starter crucial for Chinese Baijiu production, characterized by spontaneous fermentation without the inoculation of exogenous substances. However, the interactions among wheat varieties, cultivation environments, and the resulting Daqu quality remain poorly understood. This study evaluates three wheat varieties harvested from three distinct cultivation environments, examining wheat grain quality, grain-associated endophytes, and physicochemical properties and microbial communities of MT-Daqu at 0, 9, and 90 days of fermentation.

Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 6 Ameliorates Pressure Overload-Induced Cardiac Hypertrophy by Regulating Cardiomyocyte Metabolic Reprogramming.

Metabolic reprogramming is a pivotal mechanism in the pathogenesis of pathological cardiac hypertrophy. Leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6) has emerged as a significant player in cardiovascular diseases. In this study, the potential of Lgr6 to counteract pressure overload (PO)-induced cardiac hypertrophy is investigated, and the underlying mechanisms involved are elucidated.

A study on the functional design and comfort of knee pads for delivery workers.

In this study, four types of functional knee pads with structure partitions were designed for delivery workers based on leg movement patterns during delivery. By selecting three characteristic points of the knee, experiments were conducted with the subjects to objectively analyze the effects of the knee pads with different fabrics on thermal and pressure comfort. The results showed that the structural partition of the knee pads with different fabrics had a significant effect on temperature; sample 3 had the highest temperature at the end of the experiment, which increased by 0.

A multiomics dataset of paired CT image and plasma cell-free DNA end motif for patients with pulmonary nodules.

Diagnosing lung cancer at a curable stage offers the opportunity for a favorable prognosis. The emerging epigenomics analysis on plasma cell-free DNA (cfDNA), including 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) modifications, has acted as a promising approach facilitating the identification of lung cancer. And, integrating 5mC biomarker with chest computed tomography (CT) image features could optimize the diagnosis of lung cancer, exceeding the performance of models built on single feature.