Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.

Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) are important pathogens that are closely associated with hospital-acquired and community-acquired infections.

One-step narrow-thermal-cycling strand exchange amplification for sensitive detection of porcine reproductive and respiratory syndrome virus.

Porcine reproductive and respiratory syndrome (PRRS), which is primarily characterized by respiratory and reproductive dysfunction, is an epidemic disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) that has the potential to economically devastate the global swine industry. Rapid and accurate detection of PRRSV is critical for effective control of PRRS in swine. In this study, a novel one-step, highly sensitive and specific accelerated strand exchange amplification (ASEA) method for the detection of PRRSV was developed.

Scalable Production of Recombinant Adeno-Associated Virus Vectors Expressing Soluble Viral Receptors for Broad-Spectrum Inhibition of Porcine Reproductive and Respiratory Syndrome Virus Type 2.

Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a major threat to the global swine industry, causing significant economic losses. To address this, we developed a scalable recombinant adeno-associated virus (rAAV)-based strategy for the delivery of soluble viral receptors (SVRs) to treat and potentially eliminate PRRSV infections. This strategy involves fusing the virus-binding domains of two key cellular receptors, sialoadhesin (Sn4D) and CD163 (SRCR5-9), with an Fc fragment.

Metal and metal oxide nanoparticle-assisted molecular assays for the detection of Salmonella.

This paper provides a comprehensive overview of the diverse applications and innovations of nanoparticles in the detection of Salmonella. It encompasses a comprehensive range of novel methods, including efficient enrichment, nucleic acid extraction, immunoassays, nucleic acid tests, biosensors, and emerging strategies with the potential for future applications. The surface modification of specific antibodies or ligands enables nanoparticles to achieve highly selective capture of Salmonella, while optimizing the nucleic acid extraction process and improving detection efficiency.

Recent advances in metallic and metal oxide nanoparticle-assisted molecular methods for the detection of .

The detection of is of irreplaceable importance for the maintenance of public health and food safety. In the field of molecular detection, metal and metal oxide nanoparticles have demonstrated significant advantages due to their unique physicochemical properties, and their application in detection has become a cutting-edge focus of scientific research. This review systematically introduces the innovative applications of these nanoparticles in detection, including the use of magnetic nanoparticles for efficient enrichment of bacteria and precise purification of nucleic acids, as well as a variety of nanoparticle-assisted immunoassays such as enzyme-linked immunosorbent assays, lateral flow immunoassays, colorimetric methods, and fluorescence strategies.

Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.

Porcine epidemic diarrhea (PED) is a highly contagious intestinal disease caused by the porcine epidemic diarrhea virus (PEDV). The economic impact of PEDV on the global pig industry has been significant, resulting in considerable losses. This paper presents a review of the latest research progress on PEDV genome, molecular epidemiology, vaccine development, and molecular detection methods.

Advances in molecular epidemiology and detection methods of pseudorabies virus.

Pseudorabies (PR), a highly contagious disease caused by the pseudorabies virus (PRV), represents a significant threat to the global swine industry. Despite the success of developed countries in controlling the PRV epidemic through swine pseudorabies eradication programs, wild boars, as a potential source of infection, still require sustained attention and effective control measures. Concurrently, there has been considerable global attention directed towards cases of PRV infection in humans.

Efficient differentiation between Pullorum and Gallinarum by a -based PCR-HRM.

Pullorum (. Pullorum) and Gallinarum (. Gallinarum) are the biovars of serovar Gallinarum that are responsible for pullorum disease and fowl typhoid, respectively, in poultry.

Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.

Salmonella, the prevailing zoonotic pathogen within the Enterobacteriaceae family, holds the foremost position in global bacterial poisoning incidents, thereby signifying its paramount importance in public health. Consequently, the imperative for expeditious and uncomplicated detection techniques for Salmonella in food is underscored. After more than two decades of development, loop-mediated isothermal amplification (LAMP) has emerged as a potent adjunct to the polymerase chain reaction, demonstrating significant advantages in the realm of isothermal amplification.

Progress in methods for the detection of viable .

() is a prevalent enteric bacterium and a necessary organism to monitor for food safety and environmental purposes. Developing efficient and specific methods is critical for detecting and monitoring viable due to its high prevalence. Conventional culture methods are often laborious and time-consuming, and they offer limited capability in detecting potentially harmful viable but non-culturable in the tested sample, which highlights the need for improved approaches.

Rapid diagnosis of acute myocardial infarction based on reverse transcription-accelerated strand exchange amplification of miR-208a.

Acute myocardial infarction (AMI) is a prevalent cardiovascular disease associated with high morbidity and mortality, posing a significant threat to human health. Therefore, early diagnosis of AMI has become a focal point of research. MiR-208 is specifically expressed in the heart and is involved in the regulation of cardiomyocyte hypertrophy, cardiac fibrosis, and other myocardial gene expressions.

siRNA targeting gene encoding ATPase α, the ligand of Peg fimbriae, reduced Enteritidis adhesion.

enterica serovar Enteritidis (. Enteritidis) is a zoonotic pathogen that can infect both humans and animals. Among the 13 types of fimbrial operons in .

Bifunctional electrochemical biosensor based on PB-MXene films for the real-time analysis and detection of living cancer cells.

Circulating tumor cells (CTCs) are important prognostic markers for cancer diagnosis and metastasis, and their detection is an important means to detect cancer metastasis. Herein, we construct a novel bifunctional electrochemical biosensor based on the PB-MXene composite films. A simple electrostatic self-assembly approach was employed to prepare a film composed of PB nanocubes on the MXene substrates.

Advances in detection methods for viable Salmonella spp.: current applications and challenges.

Salmonella is a common intestinal pathogen that can cause food poisoning and intestinal disease. The high prevalence of Salmonella necessitates efficient and sensitive methods for its identification, detection, and monitoring, especially of viable Salmonella. Conventional culture methods need to be more laborious and time-consuming.

High-Performance Detection of Exosomes Based on Synergistic Amplification of Amino-Functionalized FeO Nanoparticles and Two-Dimensional MXene Nanosheets.

Exosomes derived from cancer cells have been recognized as a promising biomarker for minimally invasive liquid biopsy. Herein, a novel sandwich-type biosensor was fabricated for highly sensitive detection of exosomes. Amino-functionalized FeO nanoparticles were synthesized as a sensing interface with a large surface area and rapid enrichment capacity, while two-dimensional MXene nanosheets were used as signal amplifiers with excellent electrical properties.

A comparison of special intrauterine balloons and intrauterine contraceptive devices in the treatment of intrauterine adhesions.

Purpose: This study aimed to compare the efficacy of a special kind of intrauterine balloon (IUB) and that of an intrauterine contraception device (IUD) for patients with intrauterine adhesions (IUAs) after transcervical resection of adhesion (TCRA).

Methods: In this retrospective cohort study, after TCRA, 31 patients received a special IUB, and 38 patients received an IUD. The Fisher exact test, logistic regression method, Kaplan-Meier method and Cox proportional hazards regression model were used for statistical analysis.

Graphene oxide-assisted optimized narrow-thermal-cycling amplification for accurate detection of Salmonella spp.

Salmonella is a rod-shaped, Gram-negative zoonotic pathogen that poses a serious global socioeconomic and public health threat. Rapid and accurate detection of Salmonella spp. is critical for effective control of its infection.

Hierarchical Au nanoarrays functionalized 2D TiCT MXene membranes for the detection of exosomes isolated from human lung carcinoma cells.

Exosome is considered an important biomarker of liquid biopsy in early cancer screening, which can reflect the physiological and pathological status of cancer cells. Herein, we construct a novel electrochemical biosensor based on hierarchical Au nanoarray-modified 2D TiCT MXene membranes for sensitive detection of exosomes. TiCT MXene nanosheets were fabricated as the building blocks for preparing 2D membranes as the sensing platform via vacuum filtration.

Accurate, rapid and highly sensitive detection of African swine fever virus graphene oxide-based accelerated strand exchange amplification.

African swine fever is an acute, severe and highly contagious infectious disease caused by African swine fever virus (ASFV), posing a huge threat to the global swine industry. Rapid and accurate diagnostic methods are of great significance for the effective prevention and control of ASFV transmission. In this work, we established and evaluated a graphene oxide-based accelerated strand exchange amplification (GO-ASEA) method for rapid, highly sensitive, and quantitative detection of ASFV.

Tri-primer-enhanced strand exchange amplification combined with rapid lateral flow fluorescence immunoassay to detect SARS-CoV-2.

The novel coronavirus disease 2019 caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been surging rapidly around the world, which has exposed humanity to unprecedented economic, social and health impacts. To achieve efficient and accurate detection of SARS-CoV-2 on site, we developed and verified a rapid and sensitive fluorescence lateral flow immunoassay based on the innovative enhanced strand exchange amplification (ESEA-LFIA) in this study. With good amplification efficiency for short-sequence targets, ESEA is an ideal choice for the point-of-care testing of SARS-CoV-2 with a high mutation rate.

Lateral flow fluorescent immunoassay based on isothermal amplification for rapid quantitative detection of Salmonella spp.

Salmonella spp. are zoonotic pathogens of substantial public health concern. To enable detection in the field or under instrument-free conditions, we developed a rapid and robust lateral flow fluorescent immunoassay based on strand exchange amplification (SEA-LFIA) for the quantitative detection of Salmonella spp.

Rapid and sensitive detection of using fluorescent microspheres as label for immunochromatographic test strip.

Background: Bacillary dysentery caused by genus is a major cause of morbidity and mortality worldwide. In China, the popular strain was mainly (). Therefore, fluorescent microspheres (FMs)-based immunochromatographic test strip (ICTS), as a novel, reliable, sensitive and uncomplicated method, was evaluated to detect .

Virulence Gene Distribution of Pullorum Isolates Recovered from Chickens in China (1953-2015).

subspecies serovar Gallinarum biovar Pullorum ( Pullorum) has strict host specificity for poultry, and pullorum disease seriously threatens the poultry industry. Virulence genes play a central role in pathogenicity, but very few reports are available on the distribution of virulence genes in Pullorum. In this study, we investigated 304 Pullorum isolates recovered from chickens in China between 1953 and 2015 for the presence of 25 virulence genes (, , , , , , , , , , , , , , , , , , , , , , , , and ), including pathogenicity island genes, fimbriae genes, and virulence plasmid genes.

Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2.

Background: Canine parvovirus 2 (CPV-2) is one of the most common etiological agents that cause severe gastroenteritis in puppies. Early accurate diagnosis is important for infected dogs. In recent years, magnetic separation has become an efficient and useful tool for bioassays.

Serovar-Specific Polymerase Chain Reaction for Detection of Serovar Indiana.

serovar Indiana ( Indiana) is a newly emerging pathogen with high levels of drug resistance. It has become one of the most common serovars in China with a worldwide distribution, posing significant public health concerns. Detection of .