Biomedical Applications of Functionalized Composites Based on Metal-Organic Frameworks in Bone Diseases.

Every year, millions of people worldwide suffer from bone tissue damage caused by bone trauma and surgical operations, as well as diseases such as osteoporosis, osteoarthritis, osteomyelitis, and periodontitis. Bone defect repair is one of the major challenges in the field of regenerative medicine. Although bone grafts are the gold standard for treating bone defects, factors such as donor sources and immune responses limit their application.

Identification of the Glyceraldehyde-3-Phosphate Dehydrogenase () Gene Family in Revealing Its Response Characteristics to Low-Temperature and Pathogen Stress.

The glyceraldehyde-3-phosphate dehydrogenase () gene plays a pivotal role in the glycolysis/gluconeogenesis process, contributing significantly to glycosyl donor synthesis, plant growth and development, and stress responses. Bl., a heterotrophic plant in the Orchidaceae family, has its dried tubers used as the traditional Chinese medicine.

RFID-Based Real-Time Salt Concentration Monitoring with Adaptive EKF.

Salt concentration monitoring is crucial for industrial process control and wastewater management, yet existing methods often lack real-time capability or require invasive sampling. This paper presents a novel RFID wireless sensing system for noninvasive solution concentration monitoring, combining physical modeling with advanced estimation algorithms. By combining the Cole-Cole model and the slit cylindrical capacitor (SCC) model, the system establishes physics-based state-space models to characterize concentration-dependent RFID signal variations.

Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease in Rats via Gut Microbiota Remodeling and Inflammatory Suppression.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a highly prevalent chronic liver condition linked to obesity and metabolic imbalance. Alterations in the gut microbiota are increasingly recognized as contributors to its progression. , a core member of the human gut microbiota, has been linked with metabolic health, but its functional role in MASLD remains unclear.

Effect of Hot Isostatic Pressure on the Microstructure Evolution of Ti-22Al-25Nb Alloy Formed by Selective Laser Melting.

The density of SLMed (Selective Laser Melting) Ti-22Al-25Nb alloy was improved through hot isostatic pressing (HIP) treatment, and the influence of HIP and solution aging on the microstructure of Ti-22Al-25Nb alloy in the as-deposited state was examined. The results indicate that following (1100 °C + 300 MPa)/3 h-HIP, the specimen densities have risen to 99.71%, porosity has markedly decreased, and internal flaws have been eradicated.

Isolation, Characterization, and Preliminary Application of Staphylococcal Bacteriophages in Sichuan Fermentation.

Sichuan , a microbial food predominantly fermented by lactic acid bacteria and hosting a complex and diverse microbial ecosystem, serves as an ideal habitat for bacteriophages. However, relatively few studies have been conducted on isolating bacteriophages from fermented vegetables and their application in vegetable fermentation. In this study, three staphylococcal bacteriophages, ΦSx-2, ΦSs-1, and ΦSs-2, were isolated and purified from Sichuan using the spot test method.

Consensus on the Treatment of Severe Ocular Trauma and Silicone Oil-Dependent Eyes Using Foldable Capsular Vitreous Body.

Aim: To establish evidence-based guidelines for the application of the foldable capsular vitreous body (FCVB) in managing severe ocular trauma and silicone oil-dependent eyes.

Methods: We conducted a comprehensive search across multiple databases, screening, extracting, and evaluating research evidence. Experts in ocular trauma and vitreoretinal surgery provided practical insights and formulated key questions.

Deciphering key residues governing thermostability in Thermomyces lanuginosus lipase through Gibbs free energy-guided engineering.

Lipases serve as indispensable biocatalysts in many industrial applications due to their versatile catalytic abilities. To ensure their thermal resilience of the harsh biological treatment in industry, it is crucial to identify key residues which might impact thermostability. Here, computational design was adopted to decode the stability-determining residues in Thermomyces lanuginosus lipase (TLL).

Network meta-analysis of different modalities of intense pulsed light therapy in the treatment of dry eye disease induced by meibomian gland dysfunction.

Purpose: This network meta-analysis aimed to evaluate the comparative effect of five different protocols of IPL treatment in treating MGD-related DED, including optimal pulse technology [OPT]-based IPL, E > eye-based IPL, OPT-based IPL plus meibomian gland expression (MGX), E > eye-based IPL plus MGX, Eyesis-based IPL plus MGX.

Methods: PubMed, Embase, Cochrane Library, SCOPUS and China National Knowledge Infrastructure (CNKI) were searched for eligible studies published before November 10, 2022.

Results: Random network meta-analysis was conducted to estimate the differences between different protocols of IPL treatment in ocular surface disease index (OSDI), standard patient evaluation of eye dryness (SPEED), tear break-up time (TBUT), meibomian gland quality (MGQ), meibomian gland evaluation (MGE), and corneal fluorescein staining (CFS).

Contrasting patterns in diversity and community assembly of bacterioplankton and three size fractions of protists in the South China Sea.

The microbial food web plays a critical role in marine ecosystems, composed of various cell sizes of microbial organisms. Here, high-throughput sequencing of the 16S and 18S rRNA genes was conducted to detect the community structure and distribution patterns of bacterioplankton (0.2 µm-2 µm) and three size fractions of protist communities, i.

Eubacterium limosum modulates tumor microenvironments and produces antitumor metabolites active against colorectal cancer.

None declared.Conflicts of interestGut microbiota play a key role in ameliorating colorectal cancer (CRC). Eubacterium limosum is a potential probiotic with anti-CRC functions.

Gene expression profiling of SPIN1 in gastric cancer: insights into tumorigenesis and potential therapeutic targets.

Background: Gastric cancer (GC) is a prevalent malignant tumor globally, posing a significant threat to human health. The histone code reader Spindlin1 (SPIN1) has been implicated in tumorigenesis and tumor progression, however, the exact molecular mechanisms underlying these processes remain incompletely understood. The biological function and regulatory mechanisms of SPIN1 in GC remain ambiguous.

The ESCRT component FYVE4 modulates salt-stress response by strengthening the SOS1-SOS2 interaction in Arabidopsis.

The plant-specific FYVE-domain-containing protein FYVE4, a component of the endosomal sorting complex required for transport III (ESCRT-III), participates in membrane protein sorting. However, the mechanism by which FYVE4 coordinates plant growth responses to environmental stress remains unclear. In this study, we reveal a novel function of FYVE4 in positively regulating plant salt resistance by modulating the Salt Overly Sensitive (SOS) signaling pathway.

Strategic Approaches for Co-Encapsulation of Bioactive Compounds: Technological Advances and Mechanistic Insight.

In food science, the application of bioactive compounds is promising, but the deficiencies in their solubility, stability, and bioavailability seriously limit their efficacy. Co-delivery can control the release of bioactive substances and achieve synergistic effects to enhance efficacy. For this reason, co-delivery systems have emerged as a novel strategy that has attracted much attention from researchers in recent years.

Comparative and Phylogenetic Analysis of the Complete Chloroplast Genomes of Species (Fagaceae) in South China.

: In South China, species dominate mixed evergreen broadleaf forests, forming symbiotic relationships with ectomycorrhizal fungi and serving as food resources for diverse fauna, including frugivorous birds and mammals. The limited understanding of chloroplast genomes in this genus restricts our insights into its species diversity. This study investigates the chloroplast genome (cp genome) sequences from seven species, aims to elucidate their structural variation, evolutionary relationships, and functional gene content to provide effective support for future genetic conservation and breeding efforts.

Auto-Segmentation and Auto-Planning in Automated Radiotherapy for Prostate Cancer.

The objective of this study was to develop and assess the clinical feasibility of auto-segmentation and auto-planning methodologies for automated radiotherapy in prostate cancer. : A total of 166 patients were used to train a 3D Unet model for segmentation of the gross tumor volume (GTV), clinical tumor volume (CTV), nodal CTV (CTVnd), and organs at risk (OARs). Performance was assessed by the Dice similarity coefficient (), the , , , the 95% Hausdorff distance (%), and the volumetric revision degree ().

AI-Driven Transcriptome Prediction in Human Pathology: From Molecular Insights to Clinical Applications.

Gene expression regulation underpins cellular function and disease progression, yet its complexity and the limitations of conventional detection methods hinder clinical translation. In this review, we define "predict" as the AI-driven inference of gene expression levels and regulatory mechanisms from non-invasive multimodal data (e.g.

Physiological Mechanisms and Core Genes in Response to Saline-Alkali Stress in Foxtail Millet ( L.).

Soil salinization and alkalization are becoming increasingly severe in recent decades, which poses serious threats to crop production and food security in the world. Foxtail millet ( L.) is an important cereal crop in China, and it is important to elucidate its saline-alkali tolerance mechanisms for the breeding of new saline-alkali tolerant varieties.

Stress Management: How the Endoplasmic Reticulum Mitigates Protein Misfolding and Oxidative Stress by the Dual Role of Glutathione Peroxidase 8.

The endoplasmic reticulum mediates essential processes such as protein folding, transport, and post-translational modifications. Disruptions in endoplasmic reticulum function can lead to the accumulation of unfolded or misfolded proteins, initiating endoplasmic reticulum stress. This stress activates the unfolded protein response, a multifaceted signaling pathway aimed at restoring proteostasis, which is crucial for cellular survival and fate determination.

A cost-effective all-in-one halide material for all-solid-state batteries.

All-solid-state batteries require advanced cathode designs to realize their potential for high energy density and economic viability. Integrated all-in-one cathodes, which eliminate inactive conductive additives and heterogeneous interfaces, hold promise for substantial energy and stability gains but are hindered by materials lacking sufficient Li/e conductivity, mechanical robustness and structural stability. Here we present LiFeCl, a cost-effective halide material that overcomes these challenges.

Preclinical and first-in-human of purinostat mesylate, a novel selective HDAC I/IIb inhibitor, in relapsed/refractory multiple myeloma and lymphoma.

Simultaneously targeting key pathogenic drivers and remodeling of the tumor microenvironment represents a critical therapeutic strategy for relapsed or refractory (r/r) multiple myeloma (MM) and lymphoma. Purinostat mesylate (PM), a highly selective HDAC I/II binhibitor, exhibits excellent antitumor activity in MM and lymphoma cell lines and mouse models, outperforming the pan-HDAC inhibitor panobinostat or first-line/second-line multi-drug combinations. Different from panobinostat, bulk RNA-seq analysis revealed that PM suppressed essential tumor survival factors and triggered inflammation and interferon responses.

Trichinella spiralis excretory-secretory protein alleviates autoimmune thyroiditis by modulating Th17/Treg balance via the STAT3/STAT5 pathway.

Hashimoto's thyroiditis (HT) is an organ-specific autoimmune disease mediated by T cells. Patients with HT are generally treated with lifelong thyroid hormone therapy. Trichinella spiralis is recognized as a "master" of immunomodulation, but the immunomodulatory effect of its excretory-secretory (ES) proteins on HT remains unclear.

Rho GAPase protein 1 (RACGAP1) affects the proliferation and metastasis of esophageal squamous cell carcinoma cells by regulating signaling pathways: Thermal infrared medical image inspection.

This study aimed to investigate the effect of RACGAP1 on the proliferation and metastasis of esophageal squamous cell cancer cells by regulating the signaling pathway, and to evaluate the potential value of RACGAP1 in tumor diagnosis and treatment through thermal infrared medical examination. To investigate the expression level of RACGAP1 in esophageal squamous cell carcinoma and normal esophageal tissues by immunohistochemistry, and analyze the relationship between RACGAP1 expression and tumor stage, differentiation degree and prognosis of patients. The tumor cells with different expression levels of RACGAP1 were analyzed by thermal imaging with thermal infrared medical examination technique to evaluate the application value of RACGAP1 in tumor diagnosis.

Pulmonary papillary adenoma: comprehensive clinicopathologic analysis of 10 cases and molecular genetic analysis of 7 cases.

Pulmonary papillary adenoma (PPA) constitutes a relatively uncommon type of lung neoplasm, which gives rise to a diagnostic intricacy. Owing to the paucity, our understanding of this disease remains far from comprehensive, and a thorough molecular investigation around PPA has been conspicuously lacking. The current study amassed a group of 10 cases of PPA that had been precisely diagnosed.

ProtTeX: Structure-In-Context Reasoning and Editing of Proteins with Large Language Models.

Large language models (LLMs) have made remarkable progress in the field of molecular science, particularly in understanding and generating functional small molecules. This success is largely attributed to the effectiveness of molecular tokenization strategies. In protein science, the amino acid sequence serves as the sole tokenizer for LLMs.