Triple signal biosensing platform for ultrasensitive and wide-range detection of HLA gene targets.

Hyperthyroidism, a multifaceted endocrine disorder, is strongly associated with specific human leukocyte antigen (HLA) alleles, which also play a critical role in drug-induced complications such as agranulocytosis. Conventional HLA genotyping methods often face limitations in simultaneously achieving high accuracy, rapidity, and operational simplicity. To address these challenges, we developed a multimodal biosensing platform for the highly sensitive detection of alleles.

Intermediate Anthropogenic Disturbances Boost Taxonomic and Phylogenetic Diversities but Reduce Functional Diversity in Subtropical Forests of Eastern China.

Anthropogenic disturbances significantly impact plant biodiversity in subtropical forests. While prior research has primarily concentrated on taxonomic diversity, other dimensions of biodiversity, such as phylogenetic and functional diversities, remain insufficiently explored. This study simultaneously investigated these three facets of plant diversity in subtropical forests with two distinct disturbance histories in eastern China, aiming to elucidate the effects of intermediate anthropogenic disturbances on biodiversity.

Clinical Features of Chinese Patients with Thyroid Eye Disease: A Multicenter Retrospective Study.

Thyroid eye disease (TED) is a debilitating autoimmune disorder linked to thyroid dysfunction. There is limited knowledge of TED in Asian populations. This multicenter study characterizes the clinical features and treatment response of TED in a large Chinese cohort.

A rare case report of idiopathic renal infarction initially misdiagnosed as acute gastroenteritis.

Rationale: Idiopathic renal infarction refers to the sudden necrosis of kidney tissue due to a loss of blood supply, without an identifiable or specific underlying cause. It is a rare and often misdiagnosed condition owing to its nonspecific symptoms, with clinical manifestations similar to gastrointestinal or urinary disorders.

Patient Concerns: A 40-year-old male with no significant past medical history presented with sudden-onset right lower abdominal pain, nausea, vomiting, and diarrhea.

Cost-effectiveness analysis of four glucagon-like peptide-1 or glucose-dependent insulinotropic polypeptide/glucagon-like peptide-1 receptor agonists for the treatment of adult patients with overweight and obesity in China.

Aims: To evaluate the cost-effectiveness of liraglutide, semaglutide, tirzepatide, benaglutide, and lifestyle management for the treatment of obesity from the perspective of the Chinese healthcare system.

Materials And Methods: This study gathered clinical trial data from literature reviews of each treatment strategy for patients with obesity without diabetes, and efficacy data were synthesized using network meta-analysis. The data were subsequently incorporated into an constructed Markov model to simulate the lifetime treatment trajectory of patients, with a cycle length of one year.

Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice.

Objective: This study aimed to investigate the effects of PAItrap3, a novel PAI-1 inhibitor, on lipid metabolism, and autophagy pathways in diabetic mice.

Methods: db/db diabetic mice were administered PAItrap3 (5.7 mg/kg/day, IV) for 21 consecutive days, and its impact on metabolic, gene expression, and lipidomic profiles was assessed.

Risk factors for corneal nerve fiber loss in Chinese patients with type 2 diabetes mellitus.

Aims: The length of corneal nerve fibers (CNFL), a sensitive parameter for predicting early diabetic peripheral neuropathy (DPN), is influenced by abnormal glucose and lipid metabolism. We examined related glucose and lipid factors affecting CNFL in patients with type 2 diabetes mellitus (T2DM) and their diagnostic value in detecting abnormal CNFL.

Methods: Corneal nerve fiber and branch densities were evaluated in 150 patients with T2DM using corneal confocal microscopy and 80 patients underwent continuous blood glucose monitoring for 3 days.

Gut microbiota mediates semaglutide attenuation of diabetes-associated cognitive decline.

Diabetes-associated cognitive decline (DACD), characterized by cognitive impairment, is a serious complication of diabetes mellitus (DM). Research has shown that semaglutide, a novel glucagon-like peptide-1 receptor agonist, has neurotrophic and neuroprotective properties. However, a comprehensive understanding of the specific effects and underlying mechanisms of semaglutide treatment in patients with DACD remains lacking.

Thyroid Function Reference Intervals by Age, Sex, and Race : A Cross-Sectional Study.

Background: Current clinical practice uses a one-size-fits-all approach to define reference intervals for the results of diagnostic tests about thyroid function. This approach does not recognize subgroup differences according to age, sex, or race.

Objective: To identify age-, sex-, and race-specific reference intervals for the common diagnostic tests that measure thyroid function and to examine how these new reference intervals reclassify persons into disease categories when compared with current reference intervals.

Alleviating abnormal stress on compressed cranial sutures: a potential mechanism for treating and preventing deformational plagiocephaly.

Background: Abnormal stress in cranial sutures is strongly related to deformational plagiocephaly (DP) and is responsible for morphological changes in the skull, facial asymmetry, and abnormal facial tension. To evaluate the effect of biomechanical therapy on cranial sutures of DP we performed in silico analysis of normal skull in the supine position (Model 1), DP in the supine position (Model 2), and DP wearing moulding helmet (Model 3).

Methods: By utilizing 3D point cloud data, we generated finite element models (FEMs) and developed 14 cranial sutures.

Diagnosis of Tuberculous Pericarditis in Zhejiang, China: A Diagnostic Prediction Model Based on LASSO Logistic Regression.

Background And Aims: Tuberculous pericarditis (TBP) is a severe, life-threatening complication, yet its diagnosis is highly challenging due to the lack of sufficient diagnostic tools. The aim of this study was to develop and validate a diagnostic prediction model suitable for primary healthcare institutions to predict the risk of TBP.

Methods: We collected detailed medical histories, imaging examination results, laboratory test data, and clinical characteristics of patients and used the Least Absolute Shrinkage and Selection Operator (LASSO) technique combined with logistic regression analysis to construct a predictive model.

The diagnostic value of citrullinated antigens with multiple citrulline similar motif in patients with rheumatoid arthritis.

Background And Aims: Anti-cyclic citrulline peptide (anti-CCP) antibodies are among the most critical biomarkers for the diagnosis of rheumatoid arthritis (RA), with citrullinated antigens being pivotal in triggering their production. This study aimed to explore the potential diagnostic value of multiple citrulline similar-motif antigen (MCSM) in RA.

Material And Methods: A retrospective study was conducted on 135 patients with RA, 112 patients with other joint diseases (non-RA group), and 67 healthy controls from the Hangzhou Red Cross Hospital.

Effect of PAI-1 inhibitor on pancreatic islet function and hepatic insulin resistance in db/db mice.

Type 2 diabetes mellitus (T2DM) is a global health challenge, necessitating innovative antidiabetic treatments. Levels of plasminogen activator inhibitor-1 (PAI-1) are elevated in patients with T2DM and may be an important but underappreciated risk factor for diabetes. However, its relationship with T2DM remains unclear.

A comparison of the acute effects of high intensity interval training and moderate intensity continuous training on working memory and emotional state in adolescent women with subthreshold depression.

Objective: Various guidelines emphasize the cognitive and emotional benefits of physical activity. However, it is not yet clear what kind of exercise intensity is suitable for individuals with subthreshold depression. Therefore, this study aims to examine the effects of high intensity interval training (HIIT) and moderate intensity continuous training (MICT) on the working memory and emotional state.

Correlation of local and serum CircHIPK3 expressions with the progression of liver fibrosis/cirrhosis.

Background And Study Aims: This study was aimed to validate the correlation of circular RNA HIPK3 (CircHIPK3) expression in serum and tissues with the progression of liver fibrosis (LF) and liver cirrhosis (LC).

Patients And Methods: Serum CircHIPK3 expressions were detected in 120 patients with LF/LC and 120 healthy controls (HCs). CircHIPK3 expression in tissues was detected in 120 fibrotic liver tissues and compared to 57 healthy liver tissues from patients with hepatic hemangioma.

Effects of open-skill and closed-skill exercise on subthreshold depression in female adolescents: A randomized controlled trial.

Background: Subthreshold depression (SD) affects a significant proportion of adolescent females, posing a risk of major depression in later life. This study examines the effects of open-skill exercise (OSE) and closed-skill exercise (CSE) on SD, executive function (EF), and emotional states in female adolescents.

Methods: A double-blind randomized controlled trial involved 95 female adolescents (mean age = 16.

The maize gene encoding an SBP transcription factor confers osmotic resistance in transgenic .

Among the major abiotic stresses, salt and drought have considerably affected agricultural development globally by interfering with gene expression profiles and cell metabolism. Transcription factors play crucial roles in activating or inhibiting the expression of stress-related genes in response to abiotic stress in plants. In this study, the Zea mays L.

Lactate supplementation after hypoglycemia alleviates cognitive dysfunction induced by recurrent non-severe hypoglycemia in diabetic mice.

Recurrent non-severe hypoglycemia (RH) in diabetes is an independent risk factor for cognitive dysfunction. However, the mechanisms and potential therapeutic strategies remain poorly understood. In this study, we aimed to elucidate the mechanisms underlying RH-induced diabetic cognitive impairment.

Effectiveness of the frailty index in predicting recurrent pneumonia and death in long-term hospitalized patients with vascular cognitive impairment.

Objective: The aim was to predict the effectiveness of using frailty, defined by the frailty index (FI), for predicting recurrent pneumonia and death in patients 50 years and older with vascular cognitive impairment (VCI) during long-term hospitalization.

Measurements: This retrospective cohort study was conducted at a teaching hospital in western China and included VCI patients aged ≥50 years undergoing long-term hospitalization. The relevant data were collected from the electronic medical record system.

Effectiveness of sarcopenia screening markers in predicting out-of-hospital death in the oldest (≥80 years) older.

Objective: The goal of this investigation was to elucidate the correlation between sarcopenia screening indicators (aspartate transaminase/alanine transaminase (AST/ALT) and creatinine/cystatin C*100 (Cr/CysC*100)) and the risk of out-of-hospital (OFH) death among the very advanced age (≥80 years) population.

Methods: We conducted a retrospective cohort investigation, involving internal medicine inpatients aged ≥80 years of age, who sought treatment at a teaching hospital in western China. We obtained OFH mortality information from telephonic interviews.

Establishment and Validation of a Nomogram for Identifying False Positives in Xpert MTB/RIF Rifampicin Resistance Test.

Purpose: This study aimed to establish and validate a diagnostic nomogram for identifying false positives in the Xpert MTB/RIF (Xpert) for detection of rifampicin resistance (RIF-R).

Patients And Methods: In this retrospective study, we collected basic patient characteristics and various clinical information from the electronic medical record database. Patients were randomly divided into training and validation groups in a 7:3 ratio.

Advancements in understanding substantia nigra hyperechogenicity via transcranial sonography in Parkinson's disease and its clinical implications.

Amidst rising Parkinson's disease (PD) incidence in an aging global population, the need for non-invasive and reliable diagnostic methods is increasingly critical. This review evaluates the strategic role of transcranial sonography (TCS) in the early detection and monitoring of PD. TCS's ability to detect substantia nigra hyperechogenicity offers profound insights into its correlation with essential neuropathological alterations-namely, iron accumulation, neuromelanin depletion, and glial proliferation-fundamental to PD's pathophysiology.

Anionic Hofmeister Effect Regulated Conductivity in Polyelectrolyte Hydrogels for High-Performance Supercapacitor.

The Hofmeister effect not only affects the stability and solubility of protein colloids but also has specific effects on the polymer molecules. Here, the impact of the Hofmeister effect on the electrochemical properties of polyelectrolyte hydrogels at room temperature and subzero temperature studied for the first time. Polyelectrolyte hydrogels exhibit an anti-polyelectrolyte effect in low concentrations of ammonium salt, while they exhibit an obvious Hofmeister effect in high concentrations of ammonium salt.

Factors influencing false-positive results of rifampicin resistance detected by Xpert MTB/RIF: A retrospective study in Zhejiang, China.

Objective: This study aimed to explore the factors influencing false-positive results for rifampicin resistance (RIF-R) detected using Xpert MTB/RIF (Xpert).

Methods: This retrospective analysis included the clinical data of patients from September 2019 to February 2023. The chi-square and rank sum tests were used to compare differences in patient characteristics between the true-positive and false-positive groups.