Metabolic mechanism of a novel carboxylesterase YvaK in Priestia aryabhattai DPX-1 for carbamate insecticide indoxacarb detoxification.

Carbamates, organophosphates, pyrethroids, and other ester bond-containing insecticides are widely present in agricultural fields and aquatic environments, posing residue risks and threatening human health. Microbial degradation represents the primary metabolic pathway for these insecticides, yet it often generates highly toxic metabolites. In this study, we isolated a high-efficiency indoxacarb-degrading strain, Priestia aryabhattai DPX-1, which can metabolize 68 % of 5 mg/L indoxacarb within 24 h without producing the high-toxicity N-decarbomethoxylated metabolite (DCJW).

Polyvinyl chloride microplastic triggers bidirectional transmission of antibiotic resistance genes in soil-earthworm systems.

The diffusion and distribution of ubiquitous microplastics and antibiotic resistance genes (ARGs) in soil ecosystems are easily influenced by earthworm activity. However, minimal research exists on the bidirectional dissemination of ARGs in the soil-earthworm ecosystems under microplastic stress. Focusing on the typical microplastic polyvinyl chloride (PVC) microspheres in simulated soil-earthworm (Eisenia fetida) systems, we characterized the PVC-triggered interactive transmission of ARGs between earthworm guts and their dwelling soils using shotgun metagenomics and qPCR methodologies.

Molecular interaction mechanisms on (-)-epigallocatechin-3-gallate improving activities of inhibited acetylcholinesterase by selected organophosphorus pesticides in vitro & vivo.

(-)-Epigallocatechin-3-gallate (EGCG) is reported to have benefits for the treatment of Alzheimer's disease by binding with acetylcholinesterase (AChE) to enhance the cholinergic neurotransmission. Organophosphorus pesticides (OPs) inhibited AChE and damaged the nervous system. This study investigated the combined effects of EGCG and OPs on AChE activities in vitro & vivo.

Efficient and harmless removal of insecticide diazinon via the stepwise combination of biodegradation and photodegradation.

Diazinon, an organophosphorus insecticide, is predominantly removed through photodegradation and biodegradation in the environment. However, photodegradation can generate diazoxon, a highly toxic oxidation byproduct, while biodegradation is hard to complete mineralize diazinon, showing limitations in both methods. In this study, we provided an efficient strategy for the complete and harmless removal of diazinon by synergistically employing biodegradation and photodegradation.

Significantly enhanced biodegradation of profenofos by Cupriavidus nantongensis X1 mediated by walnut shell biochar.

The feasibility of using walnut shell biochar to mediate biodegradation of Cupriavidus nantongensis X1 for profenofos was investigated. The results of scanning electron microscopy, classical DLVO theory and Fourier transform infrared spectroscopy indicated that strain X1 was stably immobilized on biochar by pore filling, van der Waals attraction, and hydrogen bonding. Profenofos degradation experiments showed that strain X1 immobilized on biochar significantly decomposed profenofos (shortened the half-life by 5.

Complete biodegradation of fungicide carboxin and its metabolite aniline by Delftia sp. HFL-1.

Fungicide carboxin was commonly used in the form of seed coating for the prevention of smut, wheat rust and cotton damping-off, leading carboxin and its probable carcinogenic metabolite aniline to directly enter the soil with the seeds, causing residual pollution. In this study, a novel carboxin degrading strain, Delftia sp. HFL-1, was isolated.

Efficient Knocking Out of the Organophosphorus Insecticides Degradation Gene in X1 via CRISPR/ with Red System.

X1 is a type strain of the genus , that can degrade eight kinds of organophosphorus insecticides (OPs). Conventional genetic manipulations in species are time-consuming, difficult, and hard to control. The clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 () system has emerged as a powerful tool for genome editing applied in prokaryotes and eukaryotes due to its simplicity, efficiency, and accuracy.

Comparative Analysis of Complete Mitochondrial Genome of (Jordan and Snider, 1900), Revealing Gene Rearrangement and the Phylogenetic Relationships of Anguilliformes.

The mitochondrial genome structure of a teleostean group is generally considered to be conservative. However, two types of gene arrangements have been identified in the mitogenomes of Anguilliformes. In this study, we report the complete mitochondrial genome of (Anguilliformes (Congridae)).

Efficient biodegradation characteristics and detoxification pathway of organophosphorus insecticide profenofos via Cupriavidus nantongensis X1 and enzyme OpdB.

Profenofos residues in the environment pose a high risk to mammals and non-target organisms. In this study, the biodegradation and detoxification of profenofos in an efficient degrading strain, Cupriavidus nantongensis X1, was investigated. Strain X1 could degrade 88.

Resistance properties and adaptation mechanism of cadmium in an enriched strain, Cupriavidus nantongensis X1.

Bacterial adaption to heavy metal stress is a complex and comprehensive process of multi-response regulation. However, the mechanism is largely unexplored. In this study, cadmium (Cd) resistance and adaptation mechanism in Cupriavidus nantongensis X1 were investigated.

Enantioselective degradation of the organophosphorus insecticide isocarbophos in Cupriavidus nantongensis X1: Characteristics, enantioselective regulation, degradation pathways, and toxicity assessment.

The chiral pesticide enantiomers often show selective efficacy and non-target toxicity. In this study, the enantioselective degradation characteristics of the chiral organophosphorus insecticide isocarbophos (ICP) by Cupriavidus nantongensis X1 were investigated systematically. Strain X1 preferentially degraded the ICP R isomer (R-ICP) over the S isomer (S-ICP).

Enhanced biodegradation of organophosphorus insecticides in industrial wastewater via immobilized Cupriavidus nantongensis X1.

Chlorpyrifos is an important organophosphorus insecticide. It is highly toxic to mammals and can pollute the environment. Cupriavidus nantongensis X1 can efficiently degrade chlorpyrifos.

Enantioselective Uptake Determines Degradation Selectivity of Chiral Profenofos in X1.

Organophosphorus insecticides account for approximately 28% of the global commercial insecticide market, while 40% of them are chiral enantiomers. Chiral enantiomers differ largely in their toxicities. Enantiomers that are less active or inactive do not offer the needed efficacy but pollute the environment and cause toxicities to non-target species.

Ortho and para oxydehalogenation of dihalophenols catalyzed by the monooxygenase TcpA and NAD(P)H:FAD reductase Fre.

Dihalophenols such as dichlorophenols (DCPs) are important industrial chemical intermediates, but also persistent pollutants in the environment. Oxidative dehalogenation by microbes is an efficient biological method to degrade halophenols, but the mechanism is unclear yet. Cupriavidus nantongensis X1 was a type strain of genus Cupriavidus, and could degrade 2,4-dichlorophenol of 50 mg/L within 12 h.

Rapid Biodegradation of the Organophosphorus Insecticide Chlorpyrifos by X1.

Chlorpyrifos was one of the most widely used organophosphorus insecticides and the neurotoxicity and genotoxicity of chlorpyrifos to mammals, aquatic organisms and other non-target organisms have caused much public concern. X1, a type of strain of the genus , is capable of efficiently degrading 200 mg/L of chlorpyrifos within 48 h. This is ~100 fold faster than B-14, a well-studied chlorpyrifos-degrading bacterial strain.

Minute-Speed Biodegradation of Organophosphorus Insecticides by X1.

Organophosphorus insecticides (OPs) have been widely used to control agricultural pests, which has raised concerns about OP residues in crops and the environment. In this study, we investigated the degradation kinetics and pathways of 8 OPs by X1 and identified the enzyme via gene cloning and in vitro assays. The degradation half-life of methyl parathion, triazophos, and phoxim was only 5, 9, and 43 min, respectively.

Kinetics and Catabolic Pathways of the Insecticide Chlorpyrifos, Annotation of the Degradation Genes, and Characterization of Enzymes TcpA and Fre in Cupriavidus nantongensis X1.

Chlorpyrifos is one of the most used organophosphorus insecticides. It is commonly degraded to 3,5,6-trichloro-2-pyridinol (TCP), which is water-soluble and toxic. Bacteria can degrade chlorpyrifos and TCP, but the biodegradation mechanism has not been well-characterized.

Complete genome sequence of a novel chlorpyrifos degrading bacterium, Cupriavidus nantongensis X1.

Cupriavidus nantongensis X1 is a chlorpyrifos degrading bacterium, which was isolated from sludge collected at the drain outlet of a chlorpyrifos manufacture plant. It is the first time to report the complete genome sequence of C. nantongensis species, which has been reported as a novel species of Cupriavidus genus.

Cupriavidus nantongensis sp. nov., a novel chlorpyrifos-degrading bacterium isolated from sludge.

A Gram-stain-negative, aerobic, coccoid to small rod-shaped bacterium, designated X1T, was isolated from sludge collected from the vicinity of a pesticide manufacturer in Nantong, Jiangsu Province, China. Based on 16S rRNA gene sequence analysis, strain X1T belonged to the genus Cupriavidus, and was most closely related to Cupriavidus taiwanensis LMG 19424T (99.1 % 16S rRNA gene sequence similarity) and Cupriavidus alkaliphilus LMG 26294T (98.