Exploring Cuproptosis-Related Prognostic Signature for Hepatocellular Carcinoma: Bioinformatics and In Vitro Analyses.

Background: Hepatocellular carcinoma (HCC) is a prevalent malignant tumor with a poor prognosis. Cuproptosis, a newly identified form of programmed cell death, holds potential prognostic value in cancers, but its significance in HCC remains limited.

Methods: Differentially expressed cuproptosis-related genes (CRGs) were identified.

MAP: Masked Adversarial Perturbation for Boosting Black-Box Attack Transferability.

The transferability of adversarial examples is vital for black-box attacks, as it enables the adversary to deceive the target model without knowing its internals. Despite numerous methods focusing on transferability, they still struggle with transferring across models with distinct architectural components (e.g.

Discovery of Novel Inhibitors of DHODH via Virtual Screening, MD Simulation, and In Vitro Activity Assay.

To address the surge in infections among immunosuppressed patients and azole resistance, this study focused on developing novel inhibitors targeting dihydroorotate dehydrogenase (AfDHODH), a key enzyme in fungal pyrimidine synthesis. The three-dimensional structure of AfDHODH was constructed via homology modeling. Molecular docking, dynamics simulations, and binding free energy calculations systematically elucidated the mechanisms of existing inhibitors.

Clinical effectiveness of total alkaloids of Sophora alopecuroides L. topical solution administrated pour on against ticks in sheep.

The dermatitis could be caused by parasites infested in skin. The aim of this study was to evaluate the clinical acaricidal activity of a total alkaloid solution from Sophora alopecuroides L. The parasites in sheep were collected, photographed and initially identified, then the histology of parasites were examined through paraffin sections as ticks species.

Proteome-Wide Data Guides the Discovery of Lysine-Targeting Covalent Inhibitors Using DNA-Encoded Chemical Libraries.

Broadening the application of covalent inhibitors requires the exploration of nucleophilic residues beyond cysteine. The covalent DNA-encoded chemical library (CoDEL) represents an advanced technology for covalent drug discovery. However, its application in lysine-targeting inhibitors remains uncharted territory.

Intermediate Atrial Tachycardia During Persistent Atrial Fibrillation Ablation: A Sub-analysis of the Extent-AF Trial.

Background: The evidence supporting additional linear ablation for persistent atrial fibrillation (PerAF) remains limited. This study investigates the mechanisms of intermediate atrial tachycardia (AT), a transient state between PerAF and sinus rhythm (SR), during PerAF termination by catheter ablation, and provides evidence for these mechanisms.

Methods: We analyzed 136 patients who converted to organized AT after PerAF termination in the Extent-AF study.

LangLoc: Language-Driven Localization via Formatted Spatial Description Generation.

Existing localization methods commonly employ vision to perceive scene and achieve localization in GNSS-denied areas, yet they often struggle in environments with complex lighting conditions, dynamic objects or privacy-preserving areas. Humans possess the ability to describe various scenes using natural language, effectively inferring their location by leveraging the rich semantic information in these descriptions. Harnessing language presents a potential solution for robust localization.

Role of the admission muscle injury indicators in early coagulopathy, inflammation and acute kidney injury in patients with severe multiple injuries.

Backgrounds: Coagulopathy, inflammation and organ failure are common complications in trauma patients. This study aimed to explore the possible role of muscle injury indicators in early coagulopathy, systemic inflammatory response syndrome (SIRS), and acute kidney injury (AKI) in patients with severe multiple trauma.

Methods: A retrospective analysis was performed using trauma center patient data from 2020 to 2023.

Aggressive ablation vs. regular ablation for persistent atrial fibrillation: a multicentre real-world cohort study.

Aims: Current guidelines for the optimal ablation strategy for persistent atrial fibrillation (PerAF) remain unclear. While our previous RCT confirmed the favourable prognosis of aggressive ablation, real-world evidence is still lacking.

Methods And Results: Among 4833 PerAF patients undergoing catheter ablation at 10 centres, two groups were defined: regular ablation (PVI-only or PVI plus anatomical ablation) and aggressive ablation (anatomical plus electrogram-guided ablation), with 1560 patients each after propensity score (PS) matching.

Discovery Small-Molecule p300 Inhibitors Derived from a Newly Developed Indazolone-Focused DNA-Encoded Library.

The DNA-encoded library (DEL) is a robust tool for chemical biology and drug discovery. In this study, we developed a DNA-compatible light-promoted reaction that is highly efficient and plate-compatible for DEL construction based on the formation of the indazolone scaffold. Employing this high-efficiency approach, we constructed a DEL featuring an indazolone core, which enabled the identification of a novel series of ligands specifically targeting E1A-binding protein (p300) after DEL selection.

Discovery and Optimization of WDR5 Inhibitors via Cascade Deoxyribonucleic Acid-Encoded Library Selection Approach.

The DNA-encoded library (DEL) is a powerful hit generation tool for chemical biology and drug discovery; however, the optimization of DEL hits remained a daunting challenge for the medicinal chemistry community. In this study, hit compounds targeting the WIN binding domain of WDR5 were discovered by the initial three-cycle linear DEL selection, and their potency was further enhanced by a cascade DEL selection from the focused DEL designed based on the original first run DEL hits. As expected, these new compounds from the second run of focused DEL were more potent WDR5 inhibitors in the protein binding assay confirmed by the off-DNA synthesis.

H2H rehabilitation care promotes high quality recovery of patients with lung cancer comorbid with chronic obstructive pulmonary disease.

H2H is a patient and family-centered approach that integrates hospital and home care, emphasizing continuity, individualized rehabilitation training, and the active participation of patients and their families. However, it is still unclear whether H2H improves the efficacy for patients with COPD and lung cancer. This study investigated the efficacy of Hospital-to-Home (H2H) rehabilitation nursing for lung cancer patients with Chronic Obstructive Pulmonary Disease (COPD).

Superior reduction and immobilization of Cr(VI) in soil utilizing sulfide nanoscale zero-valent iron supported by phosphoric acid-modified biochar: Efficiency and mechanism investigation.

A novel strategy was proposed to remediate Cr(VI)-contaminated soil via phosphoric acid-modified biochar supported sulfide nanoscale zero-valent iron (SnZVI@PBC). Results of characterizations revealed that FeS shell existed in outer layer of nZVI to prevent its oxidation after sulfidation modification, and SnZVI was effectively dispersed owing to the support of PBC, accelerating the electron transport for Cr(VI) reduction. The SnZVI@PBC presented pH-dependence and fast capture for Cr(VI) with outstanding binding amount of 335.

Context and Spatial Feature Calibration for Real-Time Semantic Segmentation.

Context modeling or multi-level feature fusion methods have been proved to be effective in improving semantic segmentation performance. However, they are not specialized to deal with the problems of pixel-context mismatch and spatial feature misalignment, and the high computational complexity hinders their widespread application in real-time scenarios. In this work, we propose a lightweight Context and Spatial Feature Calibration Network (CSFCN) to address the above issues with pooling-based and sampling-based attention mechanisms.

Multi-centre, prospective randomized comparison of three different substrate ablation strategies for persistent atrial fibrillation.

Aims: The optimal strategy for persistent atrial fibrillation (PerAF) is poorly defined. We conducted a multicentre, randomized, prospective trial to compare the outcomes of different ablation strategies for PerAF.

Methods And Results: We enrolled 450 patients and randomly assigned them in a 1:1:1 ratio to undergo pulmonary vein isolation and subsequently undergo the following three different ablation strategies: anatomical guided ablation (ANAT group, n = 150), electrogram guided ablation (EGM group, n = 150), and extensive electro-anatomical guided ablation (EXT group, n = 150).

Usage of procalcitonin and sCD14-ST as diagnostic markers for postoperative spinal infection.

Objective: Identifying biomarkers for early diagnosis of postoperative spinal infection is essential to avoid complications after spine surgery. The presented study evaluated serum levels of procalcitonin (PCT), C-reactive protein (CRP), and soluble CD14 subtype (sCD14-ST) in patients who underwent spinal surgery to assess the diagnosis values of PCT and sCD14-ST.

Methods: Serum levels of PCT, CRP, and sCD14-ST were measured in 490 (289 male/201 female) patients who underwent spinal surgery (SS) before and 1 day after surgery.

Management of catheter ablation in arrhythmia patients during the coronavirus disease 2019 epidemic.

Aims: Health care providers have reduced outpatient services and delayed elective cardiac surgeries during the coronavirus disease 2019 (COVID-19) epidemic, while a large number of patients face more cardiovascular risks compared with those of the pandemic. The present study attempted to explore the management of catheter ablation in arrhythmia patients during the pandemic.

Methods And Results: From 1 January 2020 to 24 March 2020, a total of 269 arrhythmia patients hospitalized for catheter ablation under strict risk assessments and careful infection prevention were retrospectively analysed and considered for our analysis at our centre.

Five-year outcomes after catheter ablation for atrial fibrillation in patients with hypertrophic cardiomyopathy.

Background: Catheter ablation (CA) is a promising option in most patients with refractory atrial fibrillation (AF). However, data on over 5 years' outcomes with larger numbers in hypertrophic cardiomyopathy (HCM) patients with AF have not been reported. We assessed the outcome of 120 HCM patients following CA compared with a non-CA group and general patients without AF matched by HCM type with a 61.

ECNet: Efficient Convolutional Networks for Side Scan Sonar Image Segmentation.

This paper presents a novel and practical convolutional neural network architecture to implement semantic segmentation for side scan sonar (SSS) image. As a widely used sensor for marine survey, SSS provides higher-resolution images of the seafloor and underwater target. However, for a large number of background pixels in SSS image, the imbalance classification remains an issue.

RT-Seg: A Real-Time Semantic Segmentation Network for Side-Scan Sonar Images.

Real-time processing of high-resolution sonar images is of great significance for the autonomy and intelligence of autonomous underwater vehicle (AUV) in complex marine environments. In this paper, we propose a real-time semantic segmentation network termed RT-Seg for Side-Scan Sonar (SSS) images. The proposed architecture is based on a novel encoder-decoder structure, in which the encoder blocks utilized Depth-Wise Separable Convolution and a 2-way branch for improving performance, and a corresponding decoder network is implemented to restore the details of the targets, followed by a pixel-wise classification layer.

Effect of Electroacupuncture at Different Acupoints on the Expression of NMDA Receptors in ACC and Colon in IBS Rats.

Objective: To observe the effects of electroacupuncture (EA) at different acupoints on the expression of N-methyl-D-aspartate receptor (NMDA receptor ) and behaviors in irritable bowel syndrome (IBS) rats.

Methods: Wistar rats were randomly divided into blank control group (blank group, n=10) and model preparation group (n=50); experimental rat model of IBS was established by the "neonatal maternal separation and acetic acid enema" combined with "colorectal distension stimulation" method. A total of 50 IBS rats were randomly assigned to five groups of 10 each: model group, Yintang (GV29) group, Neiguan (PC6) group, Tianshu (ST25) group, and Zusanli (ST36) group.