Metabolite Profiling of Clerodendranthus spicatus by UHPLC-Q-Orbitrap HRMS and Evaluation of Antioxidant and Tyrosinase Activation Activities.

Clerodendranthus spicatus (Thunb.) C.Y.

TTLL4 mediates the PI3K/AKT/MDM2 pathway to promote hepatocellular carcinoma progression and predict patient prognosis.

Hepatocellular carcinoma (HCC) is a highly lethal and heterogeneous tumor driven by the dysregulation of multiple genes. Tubulin tyrosine ligase-like 4 (TTLL4) has been linked to tumor progression, but its specific role in HCC pathogenesis remains unclear. RNA sequencing data, somatic mutation profiles, and clinical characteristics were analyzed from TCGA, GEO, and TIMER databases.

2-Dodecyl-6-methoxycyclohexa-2,5-dien-1,4-dione alleviates liver fibrosis and improves intestinal flora and bile acid metabolism.

Background: The bioactive compound 2-Dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD), derived from the horn root of star fruit, exhibits therapeutic promise through its modulation of the TGF-β1 pathway and regulation of bile acids.

Methods: In this study, a liver fibrosis model was established in Kunming mice (KM) induced by carbon tetrachloride (CCL4), and DMDD (50 mg/kg) was administered intragastrically. HE staining, Masson staining, and Sirius staining were used to evaluate the effect of DMDD on liver fibrosis.

Mechanically Exfoliated Multilayer Graphene-Supported Ni-MOF Parallelogram Nanosheets for Enhanced Supercapacitor Performance.

Metal-organic frameworks (MOFs) are regarded as advanced supercapacitor materials owing to their high surface area, redox-active sites, and porosity. However, their insufficient charge carrier mobility remains a critical limitation for practical application. Integrating MOFs with conductive carbon substrates is an effective strategy to break through this limitation.

Leveraging Bluetooth and GPS Sensors for Route-Level Passenger Origin-Destination Flow Estimation.

Accurate estimation of passenger origin-destination (OD) matrices is critical for optimizing public transportation systems, yet conventional methods face challenges, such as incomplete alighting data, high infrastructure costs, and privacy concerns. With existing GPS sensors and the additional deployment of a single low-cost Bluetooth sensor (10-20 US dollars) per bus, the proposed method can derive passenger OD flow without requiring passengers to tap in or tap out. The GPS sensor updates the bus locations, and the Bluetooth sensor receives signals from surrounding devices, including those onboard devices and nearby external devices.

Effect of regorafenib combined with immunotherapy and arterial chemoembolization on the survival of patients with advanced hepatocellular carcinoma: a retrospective study.

Purpose: To evaluate the effect of combining regorafenib with immunotherapy, and further adding transarterial chemoembolization (TACE), on the survival rates of patients suffering from advanced hepatocellular carcinoma (HCC).

Methods: A retrospective cohort study was conducted on clinical data from 219 patients with advanced HCC treated from January 2019 to December 2020 at Zhangzhou Affiliated Hospital of Fujian Medical University. Patients were divided into two groups: regorafenib combined with immunotherapy (Group A; n = 106) and regorafenib combined with immunotherapy plus TACE (Group B; n = 113).

Machine learning predicting the effects microstructures of biomass hard carbon have on the electrochemical performance of SIBs anodes.

This study involved the development of machine learning models to investigate how the microstructures of biomass-based hard carbon (HC) influence sodium storage mechanisms and performance. The goal was to pinpoint the key microstructural features of biomass-based HC that impact the initial coulombic efficiency (ICE) and reversible capacity (RC) of sodium-ion batteries (SIBs). To achieve this, a database was established to correlate structural parameters of HC with essential sodium storage performance metrics (referred to as the Hard Carbon-SIBs, HCSs database).

TDO2 Deficiency Exacerbates the Immune Rejection Response in Rat Liver Transplantation via the Kyn-AHR Axis.

Background: The role of tryptophan 2,3-dioxygenase2 (TDO2), a key enzyme in the L-tryptophan (Trp)-kynurenine (Kyn) pathway, in liver transplant immunity is unclear. This study aims to explore the role of TDO2 in liver transplant rejection.

Methods: We used clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 to construct a TDO2 knockout rat model for liver transplant rejection.

Study on the Macroscopic Properties and Microstructure of High Fly Ash Content Alkali-Activated Fly Ash Slag Concrete Cured at Room Temperature.

Fly ash and granulated blast furnace slag are both bulk industrial solid wastes. Using these two raw materials to completely replace cement and prepare alkali-activated fly ash slag concrete (AAFSC) at room temperature can not only efficiently utilize industrial solid waste and reduce the carbon footprint, but also reduce the economic cost and technical difficulty of construction, which is of great significance for promoting the sustainable development of the concrete industry. In this article, the content of fly ash accounted for 80% of the total precursor (fly ash + slag), and a mixed solution of sodium silicate and sodium hydroxide was used as alkali activator to prepare AAFSC by curing at room temperature.

Study on the Improvement Effect of Polypropylene Fiber on the Mechanical Properties and Freeze-Thaw Degradation Performance of High Fly Ash Content Alkali-Activated Fly Ash Slag Concrete.

This article systematically investigated the improvement effect of polypropylene fiber (PPF) on the mechanical and freeze-thaw properties of alkali-activated fly ash slag concrete (AAFSC) with high fly ash content and cured at room temperature. Fly ash and slag were used as precursors, with fly ash accounting for 80% of the total mass. A mixed solution of sodium hydroxide and sodium silicate was used as alkali activator, and short-cut PPF was added to improve the performance of AAFSC.

Generation of vascularized pancreatic progenitors through co-differentiation of endoderm and mesoderm from human pluripotent stem cells.

Background: The simultaneous differentiation of human pluripotent stem cells (hPSCs) into both endodermal and mesodermal lineages is crucial for developing complex, vascularized tissues, yet poses significant challenges. This study explores a method for co-differentiation of mesoderm and endoderm, and their subsequent differentiation into pancreatic progenitors (PP) with endothelial cells (EC).

Methods: Two hPSC lines were utilized.

Multicenter Study on Transarterial Chemoembolization Combined with Radiofrequency Ablation for Early-Stage Hepatocellular Carcinoma: Primary versus Recurrent HCC.

Purpose: To evaluate the efficacy of transarterial chemoembolization (TACE) combined with radiofrequency ablation (RFA) for both primary and recurrent early-stage hepatocellular carcinoma (HCC) and to analyze the significant prognostic factors.

Patients And Methods: Data from patients with early-stage primary or recurrent HCC who underwent TACE plus RFA between August 2019 and May 2024 were collected from three major general hospitals. 158 patients were divided into a primary group and a recurrent group on the basis of their baseline characteristics.

Design of trot gait parameters planning system for parallel quadruped robot based on virtual model controller and fuzzy neural network.

The capability to achieve fast motion in varying road conditions is a crucial research aspect in the dynamic control of quadruped robot. In this study, a gait parameters planning system for quadruped robot based on virtual model controller (VMC) and fuzzy neural network controller (FNNC) is proposed. According to the expert knowledge, the FNNC is designed to help optimize the parameters in the central pattern generator and virtual model controller (CPG-VMC).

Cashew Phenol Modified Lignosulfonate Strategy for the Preparation of High-performance Dye Dispersants.

Lignin-based dye dispersants (LDD), as one of the most common lignin resource end-application product, is difficult to meet the rapidly developing needs of modern fiber dyeing due to their inherent structural defects. The efficient upgrading and modification of LDD is of great significance to the value-added application of lignin resources and the expansion of LDD sinking market. In this study, using industrial kraft lignin as raw material, cashew phenol was introduced into lignosulfonate in the form of CC bonds by ingenious utilizing the structural characteristics of lignin disordered condensation.

MBCN: A novel reference database for Effcient Metagenomic analysis of human gut microbiome.

Metagenomic shotgun sequencing data can identify microbes and their proportions. But metagenomic shotgun data profiling results obtained from multiple projects using different reference databases are difficult to compare and apply meta-analysis. Our work aims to create a novel collection of human gut prokaryotic genomes, named Microbiome Collection Navigator (MBCN).

Ultrasonic-Assisted Biphasic Aqueous Extraction of Polyphenols from Vaccinium Dunalianum Leaves: Optimization, Antioxidant, and Tyrosinase Inhibition Activities.

To optimize the ultrasonic-assisted biphasic aqueous extraction conditions for polyphenolic compounds from Vaccinium dunalianum Wight leaves and investigate their antioxidant and tyrosinase inhibition activities, single-factor experiments were conducted to investigate the effects of ethanol volume fraction (%), ammonium sulfate mass fraction (%), solid-liquid ratio (g/mL), ultrasonic temperature (°C), and ultrasonic time (min) on polyphenolic content during extraction. Based on these experiments, three key factors influencing extraction were selected for response surface methodology (RSM) optimization. The results indicated that under conditions of 26 % ethanol, 20 % ammonium sulfate, a solid-liquid ratio of 1 : 30, and extraction for 35 minutes at 50 °C, the polyphenol content reached 61.

Non-invasive early detection on esophageal squamous cell carcinoma and precancerous lesions by microbial biomarkers combining epidemiological factors in China.

Background: Microbiota may be associated with esophageal squamous cell carcinoma (ESCC) development. However, it is not known the predictive value of microbial biomarkers combining epidemiological factors for the early detection of ESCC and precancerous lesions.

Methods: A total of 449 specimens (esophageal swabs and saliva) were collected from 349 participants with different esophageal statuses in China to explore and validate ESCC-associated microbial biomarkers from genes level to species level by 16S rRNA sequencing, metagenomic sequencing and real-time quantitative polymerase chain reaction.

Adhesion Coefficient Identification of Wheeled Mobile Robot under Unstructured Pavement.

Because of its uneven and large slope, unstructured pavement presents a great challenge to obtaining the adhesion coefficient of pavement. An estimation method of the peak adhesion coefficient of unstructured pavement on the basis of the extended Kalman filter is proposed in this paper. The identification accuracy of road adhesion coefficients under unstructured pavement is improved by introducing the equivalent suspension model to optimize the calculation of vertical wheel load and modifying vehicle acceleration combined with vehicle posture data.

Design, synthesis, and inhibitory activity of hydroquinone ester derivatives against mushroom tyrosinase.

Tyrosinase is a widely distributed copper-containing enzyme found in various organisms, playing a crucial role in the process of melanin production. Inhibiting its activity can reduce skin pigmentation. Hydroquinone is an efficient inhibitor of tyrosinase, but its safety has been a subject of debate.

6'--Caffeoylarbutin from Quezui Tea: A Highly Effective and Safe Tyrosinase Inhibitor.

Tyrosinase is vital in fruit and vegetable browning and melanin synthesis, crucial for food preservation and pharmaceuticals. We investigated 6'--caffeoylarbutin's inhibition, safety, and preservation on tyrosinase. Using HPLC, we analyzed its effect on mushroom tyrosinase and confirmed reversible competitive inhibition.

FEN1 Promotes Hepatocellular Carcinoma Progression by Activating Cell Cycle Transition from G2 To M Phase.

Flap endonuclease 1 (FEN1) is a structure-specific nuclease that is involved in the occurrence and development of various types of tumors. Previous studies have shown that FEN1 plays an important role in the development of hepatocellular carcinoma, however, the molecular mechanisms remain fully elucidated, especially its effect on the cell cycle of hepatocellular carcinoma has not been investigated. In this study, via bioinformatics prediction and clinical specimen verification, we confirmed that FEN1 was highly expressed in HCC and correlated with poor prognosis.

Cuproptosis-related molecular patterns and gene (ATP7A) in hepatocellular carcinoma and their relationships with tumor immune microenvironment and clinical features.

Background: Cuproptosis has been studied in various aspects as a new form of cell death.

Aims: We hope to explore the molecular patterns and genes related to cuproptosis in evaluating and predicting the prognosis of hepatocellular carcinoma (HCC), as well as the impact of tumor immune microenvironment.

Methods And Results: Sixteen cuproptosis related gene (CRGs) and cuproptosis related molecular and gene characteristics were comprehensively analyzed from 492 HCC samples.

From imaging to clinical outcome: dual-region CT radiomics predicting FOXM1 expression and prognosis in hepatocellular carcinoma.

Background: Liver cancer, especially hepatocellular carcinoma (HCC), remains a significant global health challenge. Traditional prognostic indicators for HCC often fall short in providing comprehensive insights for individualized treatment. The integration of genomics and radiomics offers a promising avenue for enhancing the precision of HCC diagnosis and prognosis.

Prediction of prognosis, immune infiltration, and personalized treatment of hepatocellular carcinoma by analysis of cuproptosis-related long noncoding RNAs and verification .

Background: The correlations between cuproptosis and long noncoding RNAs (lncRNAs) with the tumor microenvironment (TME), immunotherapy, and some other characteristics of hepatocellular carcinoma (HCC) remain unclear.

Methods: Sixteen cuproptosis regulators and 356 cuproptosis-related lncRNAs (CRLnc) were identified from 374 HCC profiles in The Cancer Genome Atlas (TCGA) database. Six differentially expressed CRLnc were selected, and a prognostic risk model based on the CRLnc signature (CRLncSig) was constructed.