Integrated transcriptomic and metabolomic analysis reveals stage-associated molecular profiles in Ophiocordyceps sinensis.

Unlabelled: is globally recognized for its exceptional nutritional and medicinal properties. Variations in the edible qualities and tonic values of at different harvesting stages remain poorly understood in terms of compositional changes and regulatory mechanisms. Utilizing UPLC-MS/MS and transcriptome sequencing (RNA-seq), this study unveiled discrepancies in metabolite accumulation and gene expression of across various harvesting stages.

Discovery and biochemical characterization of prenyltransferases in the biosynthetic pathway of hericenones from Hericium erinaceus.

Hericenones, neurotrophic meroterpenoids from the medicinal mushroom Hericium erinaceus, exhibit remarkable nerve growth factor-enhancing properties with therapeutic potential for neurodegenerative disorders. Biosynthesis of these compounds requires prenyltransferase (PT)-mediated geranylation of orsellinic acid (OA, 1), though the fungal enzymes catalyzing this pivotal step remain uncharacterised. Through comprehensive genome mining of H.

Genomic Sequencing and Characterization of Two Species from the Qinling Region: Insights into Evolutionary Dynamics and Secondary Metabolite Potential.

mushrooms, common bulk edible fungi, have considerable culinary and medicinal value. The Qinling region, represented by Zhashui County, is the main production area of mushrooms in China. In this study, two wild strains, M12 and M13, selected from the Qinling region for their desirable horticultural traits after domestication, were sequenced and characterized.

Trichaptum biforme (Pallidohirschioporus biformis) genome decoding provides insights into carbohydrate degradation and polysaccharide synthesis.

Trichaptum biforme is a white-rot fungus that plays a key role in the process of cellulose degradation. In this study, we employed a suite of techniques to sequence the genome of T. biforme, and achieved high-quality assembly and detailed annotation.

The complete mitochondrial genome of medicinally important wood-decaying fungus within the family Incrustoporiaceae, Polyporales.

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Exploring the molecular tapestry of secondary metabolites: chemical structures, activities, and biosynthesis.

mushrooms are esteemed as a rare and highly valuable resource for both culinary and medicinal purposes. Ancient medical classics have documented their beneficial effects on conditions such as indigestion, loss of appetite, and neurological disorders. Modern phytochemical research into their secondary metabolites has led to the discovery of numerous bioactive compounds with significant biological activities.

Haplotype-Phased Chromosome-Level Genome Assembly of , a Typical Traditional Chinese Medicine Fungus.

This study presents the first comprehensive genomic analysis of , a classical folk medicine and newly identified macrofungus from the Qinling Mountains. Utilizing advanced sequencing technologies, including PacBio HiFi and Hi-C, we achieved a high-quality chromosome-level genome assembly. The genome, sized at 39.

Metabolomics and Transcriptomics Reveal the Effects of Different Fermentation Times on Antioxidant Activities of .

is a fungus that is cultured through fermentation from wild Chinese cordyceps. While studies have examined its metabolites, the evaluation of its antioxidant capacity remains to be conducted. The antioxidant results of indicate that the ferric ion-reducing antioxidant power (FRAP), antioxidant capacity (2.

Enhancing Mushroom Yield and Quality Through the Amendment of Soil Physicochemical Properties and Microbial Community with Wood Ash.

mushroom is a nutritionally rich and rare edible fungus. The traditional cultivation model, which relies on expanding the cultivation area to meet market demand, is no longer sufficient to address the rapidly growing market demand. Enhancing the yield and quality of without increasing the cultivation area is an intractable challenge in the development of the mushroom industry.

Systematic analysis of natural topoisomerase I inhibitors from by ultrafiltration-UPLC-ESI-MS/MS, pharmacophore modelling, and molecular dynamics simulation.

This study conducted a systematic analysis to explore natural DNA topoisomerase I (topo I) inhibitors from (). Crude extract of exhibited notable toxic and anti-proliferative effects on A549 cells. A total of 36 components were identified using bioaffinity ultrafiltration UPLC-ESI-MS/MS.

Chemical structures, biological activities, and biosynthetic analysis of secondary metabolites of the Diatrypaceae family: A comprehensive review.

The family Diatrypaceae is a less well-known group within the order Xylariales (Ascomycota). Initially, the focus on its metabolites was related to the pathogenicity of one of its members, . To date, a total of 254 natural products have been identified from Diatrypaceae strains.

The chemical structures, biosynthesis, and biological activities of secondary metabolites from the culinary-medicinal mushrooms of the genus Hericium: a review.

Fungal phytochemicals derived from higher fungi, particularly those from the culinary-medicinal genus Hericium, have gained significant attention in drug discovery and healthcare. This review aims to provide a comprehensive analysis of the chemical structures, biosynthetic pathways, biological activities, and pharmacological properties of monomeric compounds isolated from Hericium species. Over the past 34 years, 253 metabolites have been identified from various Hericium species, including cyathane diterpenes, alkaloids, benzofurans, chromenes, phenols, pyrones, steroids, and other miscellaneous compounds.

Evaluation of Phenazine Derivatives from the Lichen-Associated as Potent Antineuroinflammatory Agents In Vitro and In Vivo.

Eighteen nitrogen-containing compounds (-) were isolated from cultures of the lichen-associated collected from the Qinghai-Tibet Plateau, including seven phenazine derivatives with three new ones, named subphenazines A-C (-), two new furan pyrrolidones (-), and nine known alkaloids. The structures were elucidated by spectroscopic data analysis, and absolute configurations were determined by single-crystal X-ray diffraction and ECD calculations. The phenazine-type derivatives, in particular compound , exhibited significantly better antineuroinflammatory activity than other isolated compounds (-).

Genome-wide characterization and metabolite profiling of Cyathus olla: insights into the biosynthesis of medicinal compounds.

Cyathus olla, belonging to the genus Cyathus within the order Agaricales, is renowned for its bird's nest-like fruiting bodies and has been utilized in folk medicine. However, its genome remains poorly understood. To investigate genomic diversity within the genus Cyathus and elucidate biosynthetic pathways for medicinal compounds, we generated a high-quality genome assembly of C.

Evaluation of Quality in 15 Production Areas Using Metabolomics and the Membership Function Method.

is a precious medicinal and edible fungus, which is widely used in body health care and disease prevention. The current research focuses on the comparison of metabolite characteristics between a small number of samples and lacks a comprehensive evaluation of the quality of in a large-scale space. In this study, LC-MS/MS, principal component analysis (PCA), hierarchical cluster analysis (HCA), and the membership function method were used to comprehensively evaluate the characteristics and quality of metabolites in 15 main producing areas of in China.

Chemical Structures, Biological Activities, and Biosynthetic Analysis of Secondary Metabolites from Mushrooms: A Review.

mushrooms are an important genus in the Agaricaceae family, belonging to the order Agaricales of the class Basidiomycota. Among them, is a common mushroom for mass consumption, which is not only nutritious but also possesses significant medicinal properties such as anticancer, antibacterial, antioxidant, and immunomodulatory properties. The rare edible mushroom, , contains unique agaricol compounds with significant anticancer activity against liver cancer.

Functional Characterization of Sesquiterpene Synthases and P450 Enzymes in for Biosynthesis of Spiro [4.5] Decane Terpene.

, a popular edible mushroom, contains sesquiterpenes with potential health benefits. We characterized 12 sesquiterpene synthases and one P450 enzyme in using as a heterologous expression system, culminating in the biosynthesis of 16 distinct sesquiterpene compounds. An enzyme encoded by the gene responsible for the synthesis of the spiro [4.

Biochemical characterization of a multiple prenyltransferase from Tolypocladium inflatum.

Prenylation plays a pivotal role in the diversification and biological activities of natural products. This study presents the functional characterization of TolF, a multiple prenyltransferase from Tolypocladium inflatum. The heterologous expression of tolF in Aspergillus oryzae, coupled with feeding the transformed strain with paxilline, resulted in the production of 20- and 22-prenylpaxilline.

Natural and engineered xylosyl products from microbial source.

Glycosylation is a prevalent post-modification found in natural products and has a significant impact on the structural diversity and activity variation of natural products. Glucosylation is the most common type of glycosylation, whereas xylosylation is relatively rare. Despite their unique chemical structures and beneficial activities, xylosylated natural products from microorganisms have received little attention.

Effect of Air Drying on the Metabolic Profile of Fresh Wild and Artificial .

Fresh and dried are widely used by the public for medicinal and health purposes. However, the differences between them have not been examined. In this study, fresh wild and artificial (WFC and AFC) were dried to obtain dried wild and artificial (WDC and ADC).

The alkynyl-containing compounds from mushrooms and their biological activities.

Mushrooms have been utilized by humans for thousands of years due to their medicinal and nutritional properties. They are a crucial natural source of bioactive secondary metabolites, and recent advancements have led to the isolation of several alkynyl-containing compounds with potential medicinal uses. Despite their relatively low abundance, naturally occurring alkynyl compounds have attracted considerable attention due to their high reactivity.

Comparative Genomic Analysis and Metabolic Potential Profiling of a Novel Culinary-Medicinal Mushroom, (Basidiomycota).

is an emerging species in the genus with few members. Despite being highly regarded due to its rarity, knowledge about remains limited. In this study, we sequenced, de novo assembled, and annotated the complete genome of NPCB A08, isolated from the Qinling Mountains in Shaanxi, China, using the Illumina NovaSeq and Nanopore PromethION technologies.

Molecular properties, structure, neurotrophic and anti-inflammatory activities of cultured secondary metabolites from the cultures of the mushroom CBPFE A06.

Two previously undescribed natural cyathane diterpenoids Me-dentifragilin A () and Epi-neocyathin O (), and three known cyathane diterpenoids -, cyathin O, neocyathin P, and cyathin I, were isolated from the rice medium of the CBPFE A06. Their structures were established by NMR spectra, and HR-ESI-MS. Compounds - displayed encouraging neurotrophic activity in PC-12 cells at doses of 5 µM.

Combination of bioaffinity ultrafiltration-UFLC-ESI-Q/TOF-MS/MS, in silico docking and multiple complex networks to explore antitumor mechanism of topoisomerase I inhibitors from Artemisiae Scopariae Herba.

Background: Artemisiae Scopariae Herba (ASH) has been widely used as plant medicine in East Asia with remarkable antitumor activity. However, the underlying mechanisms have not been fully elucidated.

Methods: This study aimed to construct a multi-disciplinary approach to screen topoisomerase I (topo I) inhibitors from ASH extract, and explore the antitumor mechanisms.