Integrated transcriptomic and metabolomic analysis reveals stage-associated molecular profiles in Ophiocordyceps sinensis.

Unlabelled: is globally recognized for its exceptional nutritional and medicinal properties. Variations in the edible qualities and tonic values of at different harvesting stages remain poorly understood in terms of compositional changes and regulatory mechanisms. Utilizing UPLC-MS/MS and transcriptome sequencing (RNA-seq), this study unveiled discrepancies in metabolite accumulation and gene expression of across various harvesting stages.

Integrated Amino Acid Profiling and 4D-DIA Proteomics Reveal Protein Quality Divergence and Metabolic Adaptation in Species.

To explore the differences in protein quality among classic medicinal entomopathogenic fungi and to evaluate their metabolic adaptability, we analyzed the amino acid composition and proteomic characteristics of (CS), (CM), and (CC). Quantitative analysis showed CM contained the highest crude protein and lysine, methionine, threonine, and valine. CS adapted to high-altitude hypoxia and exhibited lower protein but elevated leucine, isoleucine, and histidine contents, which may contribute to membrane stabilization and oxidative stress resistance.

Characteristics of lipid accumulation induced by high-altitude environment improve the total antioxidant capacity of Ophiocordyceps sinensis.

Ophiocordyceps sinensis is a traditional Chinese herbal medicine and nutritional supplement, which is rich in functional components beneficial to health. The biosynthesis of these components is affected by environment factors, especially lipids. This study analyzed the lipid mass spectrometry characteristics and total antioxidant capacity (TAC) of O.

Electronic vascular conduit for in situ identification of hemadostenosis and thrombosis in small animals and nonhuman primates.

Patients suffering from coronary artery disease (CAD) or peripheral arterial disease (PAD) can benefit from bypass graft surgery. For this surgery, arterial vascular grafts have become promising alternatives when autologous grafts are inaccessible but suffer from numerous postimplantation challenges, particularly delayed endothelialization, intimal hyperplasia, high risk of thrombogenicity and restenosis, and difficulty in timely detection of these subtle pathological changes. We present an electronic vascular conduit that integrates flexible electronics into bionic vascular grafts for in situ, real-time and long-term monitoring for hemadostenosis and thrombosis concurrent with postoperative vascular repair.

Uncovering proteome variations and concomitant quality changes of different drying methods by 4D-DIA structural proteomics.

Introduction: is a fungus, serves dual purposes as both a medicinal herb and a food source. Due to its high water content, fresh is difficult to preserve, necessitating the drying necessary to process .

Methods: Using 4D-DIA proteomics, researchers analyzed the proteome profiles of fresh (CK) under three different drying conditions: vacuum freeze-drying (FD), oven-drying (OD), and air-drying (AD).

Influence of Drying Methods on the Morphological Features, Microstructural Properties, and Antioxidant Performance of : A Metabolomic Analysis.

() has garnered increasing attention as an ingredient in both the pharmaceutical and food industries. Depending on the drying method, the accumulation of metabolites can greatly affect the quality. This research employed an untargeted metabolomics (LC-MS/MS) strategy to elucidate the similarities and differences in the morphological characteristics, microstructure, antioxidant capacity, and metabolic profiles of subjected to three distinct drying methods: natural air-drying (YG), oven-drying (HG), and vacuum freeze-drying (DG).

Metabolomics and Transcriptomics Reveal the Effects of Different Fermentation Times on Antioxidant Activities of .

is a fungus that is cultured through fermentation from wild Chinese cordyceps. While studies have examined its metabolites, the evaluation of its antioxidant capacity remains to be conducted. The antioxidant results of indicate that the ferric ion-reducing antioxidant power (FRAP), antioxidant capacity (2.

Evaluation of Lipid Changes During the Drying Process of by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)-Based Lipidomics Technique.

Comprehensive analysis of the lipid content in samples is essential for optimizing their effective use. Understanding the lipid profile can significantly enhance the application of this valuable fungus across various fields, including nutrition and medicine. However, to date, there is limited knowledge regarding the effects of different drying methods on the quality of lipids present in .

Analysis of Metabolic Profiles and Antioxidant Activity of Chinese Cordyceps, and Based on Untargeted Metabolomics.

Chinese cordyceps (GL) is a traditional medicinal fungus, with (, BL) and (, JSB) being fungi isolated from wild Chinese cordyceps. These three species share similar chemical composition and pharmacological effects. Existing studies have primarily compared the metabolites of Chinese cordyceps and , overlooking the assessment of antioxidant capacity in Chinese cordyceps, , and .

Effects of Different Drying Methods on Amino Acid Metabolite Content and Quality of by LC-MS/MS Combined with Multivariate Statistical Methods.

, a medicinal fungus utilized in traditional Chinese medicine, exhibits a range of biological activities and pharmacological functions. In this study, we determined the amino acid composition of 94 amino acids in using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Fresh samples of were analyzed under three different drying methods: vacuum freeze drying (DG), oven drying (HG), and air drying (YG).

Accumulation Characteristics of Natural Metabolites Driven by Environmental Factors.

The environment is an important factor affecting the composition and abundance of metabolites in , which indirectly determines its edible function and medicinal potential. This study integrated metabolomics and redundancy analysis (RDA) to analyze the metabolite profile characteristics and key environmental factors influencing in various production areas. A total of 700 differentially accumulated metabolites (DAMs) were identified, primarily comprising lipids, organic acids, and organoheterocyclic compounds.

Analysis of metabolic spectrum characteristics of naturally and cultivated Ophiocordyceps sinensis based on non-targeted metabolomics.

The analysis of the differences in metabolic profiles between naturally Ophiocordyceps sinensis (NO) and cultivated Ophiocordyceps sinensis (CO) is an essential process for the medicinal value mining of Ophiocordyceps sinensis. Non-targeted metabolomics was used to compare the differences in metabolite composition and abundance between NO and CO. Total metabolite composition found that NO is rich in organic acids and derivatives, and CO is rich in lipids and lipid-like molecules.

Metabolomics Combined with Physiology and Transcriptomics Reveal the Response of to Key Metabolic Pathways and Its Degradation Mechanism during Subculture.

During the subculture of filamentous fungi, obvious signs of degradation occur which affect the growth and development of the strain, change the content of metabolites, and interfere with gene expression. However, the specific molecular mechanism of filamentous fungi degradation is still unclear. In this study, a filamentous fungus was used as the research object, and it was continuously subcultured.

Complete chloroplast genomes and phylogenetic relationships of Pedicularis chinensis and Pedicularis kansuensis.

The complete cp genomes of Pedicularis chinensis (GenBank accession number: OQ587614) and Pedicularis kansuensis (GenBank accession number: OQ587613) were sequenced, assembled, and annotated. Their chloroplast (cp) genome lengths were 146,452 bp, and 146,852 bp, respectively; 120 and 116 genes were identified, comprising 75 and 72 protein-coding genes (PCGs), 37 and 36 transfer RNA (tRNA) genes, and 8 and 8 ribosomal RNA (rRNA) genes, for P. chinensis and P.

Effects of Drying Methods on Morphological Characteristics, Metabolite Content, and Antioxidant Capacity of .

is a rare and endangered medicinal herb in China and a typical medicinal and food plant. Most of the research related to focuses on its pharmacological effects, artificial cultivation and clinical applications. However, there are few comprehensive evaluations on the quality of under different drying methods.

Evaluation of Quality in 15 Production Areas Using Metabolomics and the Membership Function Method.

is a precious medicinal and edible fungus, which is widely used in body health care and disease prevention. The current research focuses on the comparison of metabolite characteristics between a small number of samples and lacks a comprehensive evaluation of the quality of in a large-scale space. In this study, LC-MS/MS, principal component analysis (PCA), hierarchical cluster analysis (HCA), and the membership function method were used to comprehensively evaluate the characteristics and quality of metabolites in 15 main producing areas of in China.

Revealing Medicinal Constituents of Based on Non-Targeted Metabolomics and 16S rDNA Gene Sequencing Technology.

is a medicinal plant with a long history, but there are few studies on the effects of its medicinal components and endophytic bacteria on the accumulation of secondary metabolites. Therefore, in this study, non-targeted metabolomics techniques and 16s rDNA techniques were used to study from different regions. A total of 1290 metabolites and 437 differential metabolites were identified from all samples.

Effect of Air Drying on the Metabolic Profile of Fresh Wild and Artificial .

Fresh and dried are widely used by the public for medicinal and health purposes. However, the differences between them have not been examined. In this study, fresh wild and artificial (WFC and AFC) were dried to obtain dried wild and artificial (WDC and ADC).

Characterization of Metabolite Landscape Distinguishes Medicinal Fungus and other by UHPLC-Q Exactive HF-X Untargeted Metabolomics.

represent a valuable class of medicinal fungi with potential utilization. The overexploitation and resource scarcity of (CS) have led to the emergence of such as (CM) and (CC) as substitutes. The medicinal value of CS is often considered superior to other , potentially owing to differences in active ingredients.

Complete Chloroplast Genomes of , , and : Genome Structures and Comparative and Phylogenetic Analyses.

plants are widely distributed in Asia and Europe; however, their complex phylogenetic relationships have led to many difficulties in phylogenetic studies and interspecific identification. In this study, we assembled, annotated, and analyzed the chloroplast genomes of three plants: , , and . The results showed that the full-length sequences of the three plants were 152,561 bp, 151,452 bp, and 152,293 bp, respectively, which represent the typical quadripartite structure, and the genomes were relatively conserved.

Phylogenetic relationship and characterization of the complete chloroplast genome of subsp. Maximowicz 1877, an ornamental and medicinal plant in China.

As one of the top 10 famous flowers in Chinese tradition, subsp. known as 'beauty in flowers,' which has high ornamental and medicinal value. The complete chloroplast (cp) genome of subsp.

Unconstrained Piezoelectric Vascular Electronics for Wireless Monitoring of Hemodynamics and Cardiovascular Health.

The patient-centered healthcare requires timely disease diagnosis and prognostic assessment, calling for individualized physiological monitoring. To assess the postoperative hemodynamic status of patients, implantable blood flow monitoring devices are highly expected to deliver real time, long-term, sensitive, and reliable hemodynamic signals, which can accurately reflect multiple physiological conditions. Herein, an implantable and unconstrained vascular electronic system based on a piezoelectric sensor immobilized is presented by a "growable" sheath around continuously growing arterial vessels for real-timely and wirelessly monitoring of hemodynamics.

Self-Driven Electrical Stimulation-Promoted Cancer Catalytic Therapy and Chemotherapy Based on an Implantable Nanofibrous Patch.

The efficacy of cancer catalytic therapy is still hindered by the inefficient generation of reactive oxygen species (ROS). Herein, we report a self-driven electrical stimulation-promoted cancer catalytic therapy and chemotherapy by integrating a human-driven triboelectric nanogenerator (TENG) with an implantable and biodegradable nanofibrous patch. The gelatin/polycaprolactone nanofibrous patch incorporates doxorubicin (DOX) and graphitic carbon nitride (g-CN), in which the peroxidase (POD)-like activity of g-CN to produce hydroxyl radical (OH) can be distinctly enhanced by the self-driven electrical stimulation for 4.

Medium optimization for high mycelial soluble protein content of using response surface methodology.

is widely utilized due to its pharmaceutical value. Mycelial protein forms a key active component of and determines the medicinal potential of fungus. Here, we describe the development of an optimized fermentation medium to obtain more mycelial soluble protein from using response surface methodology (RSM) and investigate the increased mycelial protein content using transcriptomics.