Coalition Resection and Extra-Osseous Talotarsal Stabilization for Talocalcaneal Coalition With Pes Planus Deformity: A Retrospective Study.

Objective: The treatment of talocalcaneal coalition (TCC) with pes planus deformity in adolescent patients still presents challenges. With the pes planus deformity untreated, the final clinical outcome would be compromised. Therefore, simultaneous correction of pes planus deformity seems to be of great importance.

Deacetylation of ATG16L1 is required for LC3-associated lysosomal microautophagy.

Microautophagy is a selective cellular process in which endolysosomes directly engulf cytoplasmic cargo through membrane invagination. The regulatory mechanisms governing microautophagy remain poorly understood. Here, we identified the deacetylation of ATG16L1 as a critical regulator of LC3-associated lysosomal microautophagy.

Prior Effective Sample Size When Borrowing on the Treatment Effect Scale.

With the robust uptick in the applications of Bayesian external data borrowing, eliciting a prior distribution with the proper amount of information becomes increasingly critical. The prior effective sample size (ESS) is an intuitive and efficient measure for this purpose. The majority of ESS definitions have been proposed in the context of borrowing control information.

Heterogeneity and co-occurrence of resistance mechanisms in EGFR-TKI-induced NSCLC to SCLC transformation: a case report.

Background: Transformation into small-cell lung carcinoma (SCLC) is a common acquired resistance mechanism to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs). Re-tumor biopsy is crucial for identifying the definite tumor resistance mechanism. However, multiple mechanisms may occur simultaneously during TKI treatment.

Immune cell landscape in a human decedent receiving a pig liver xenograft.

Recent advancements in genetically engineered pigs have spurred research into pig-to-human xenotransplantation, particularly involving heart, kidney and liver. Here we characterize immune cell populations cells in both peripheral blood and transplanted liver in a human decedent who received a pig liver xenograft and who was monitored over the course of 10 days. Using single-cell RNA sequencing and spatial RNA sequencing, we found that T cells were progressively activated in the peripheral blood, whereas γδT cells and exhausted T cells infiltrated the pig liver extensively, indicating impaired adaptive immunity.

Correction: Baicalin-modifed polyethylenimine for miR-34a efficient and safe delivery.

[This corrects the article DOI: 10.3389/fbioe.2023.

An injectable calcium sulfate-monetite biphasic cement for the treatment of critical-sized calvarial defects.

The critical bone defect is a common clinical challenge worldwide, characterized by long recovery times, a substantial risk of infection, and high disability rates. There remains a significant demand for synthetic bone-repairing materials to address this issue. In this study, porous monetite was synthesized through the reaction between monocalcium phosphate monohydrate and β-tricalcium phosphate, using paraffin microspheres as pore-forming agents.

DLP 3D printing of hyperelastic photocurable perivascular scaffolds enabling patient-specific vascular remodeling.

Photopolymerization-based 3D printing, enabled the fabrication of complex, patient-specific scaffolds with high resolution and spatial precision. However, most photocurable biomaterials exhibited mechanical mismatch with soft tissues such as vascular, cartilage, and tendon tissues. To address this limitation, we developed a biodegradable and elastomeric resin (A-PLCL/4SH), composed of methacrylate-functionalized poly(L-lactide-co-ε-caprolactone) (A-PLCL) and pentaerythritol tetra(3-mercaptopropionate) (PETA-4SH), which enabled high-fidelity digital light processing (DLP) 3D printing.

[Heat-reinforcing needling inhibits oxidative stress injury via modulating the Sirt1/FoxO1 pathway in rheumatoid arthritis rats with cold syndrome].

Objectives: To observe the effects of heat-reinforcing needling on inflammation and oxidative stress in rheumatoid arthritis (RA) model rats with cold syndrome, so as to analyze the mechanism of heat-reinforcing needling in improving RA.

Methods: Male SD rats were randomly divided into blank group, model group, heat-reinforcing needling group and resveratrol group according to random number table method, with 12 rats in each group. The rat model of RA with cold syndrome was established by subcutaneous injection of bovine type II collagen and complete Freund adjuvant combined with the stimulation of cold and wet environment.

Comprehensive Assessment and Freeze-Thaw Durability Prediction of Wet-Sprayed Concrete for Cold-Region Tunnels.

This study examines freeze-thaw deterioration patterns and predicts the service life of wet-sprayed concrete with composite cementitious materials in cold-region tunnels. The microstructure and particle size distribution of four materials (cement, fly ash, silica fume, and mineral powder) were analyzed. Subsequent tests evaluated the rebound rate, mechanical properties, and durability of wet-sprayed concrete with various compositions and proportions of cementitious materials, emphasizing freeze-thaw resistance under cyclic freezing and thawing.

Dose and local efficacy analysis of iodine-125 seed implantation therapy for lung tumors.

Objective: To explore the dose and local efficacy of iodine-125 seed implantation therapy for lung tumors.

Methods: The clinical data of 85 patients with lung tumors who underwent iodine-125 seed implantation therapy were retrospectively analyzed. The impact of prescription dose D90 (minimum peripheral dose received by the 90% target volume) on the local treatment effect six months after seed implantation was analyzed, and the critical value for predicting efficacy was determined.

Soluble programmed cell death ligand-1 as a predictive biomarker for severity and poor prognosis in pulmonary tuberculosis.

Background: We aimed to assess whether soluble programmed death-ligand 1 (sPD-L1) could serve as a new biomarker for PTB.

Methods: Plasma sPD-L1 levels in the discovery cohort were analyzed through flow cytometry and validated by sandwich ELISA. Pleural effusion sPD-L1 levels were measured using ELISA.

A Cellular Coupling Strategy Leveraging Pathway Modularization and Cofactor Regeneration for the Biosynthesis of 3'-Sialyllactose.

3'-Sialyllactose is a major sialylated human milk oligosaccharide that plays an important role in promoting infant development and supporting various physiological functions critical for early life. In this study, -acetylneuraminic acid and 3'-sialyllactose were successfully synthesized utilizing four genes across three modules through a whole-cell catalysis. Among these, Module III was employed to facilitate the conversion of cytidine-5'-monophosphate to cytidine 5'-triphosphate utilizing the intrinsic enzyme system of .

Aberrant angiogenic signaling in HCC: therapeutic targeting and drug resistance.

Liver cancer ranks as the sixth most prevalent malignancy globally, with Hepatocellular Carcinoma (HCC) constituting the predominant subtype, thereby imposing a significant burden on public health and presenting limited therapeutic options. Despite ongoing efforts to innovate treatment modalities, anti-angiogenesis therapy continues to be the primary strategy for managing HCC. Angiogenesis is a pivotal process within the tumor microenvironment, characterized by the formation of new blood vessels that provide essential nutrients and oxygen to proliferating tumors, thereby facilitating their growth and potential metastasis.

Risk factors associated with health literacy among community residents in China based on multiple correspondence analysis and ordinal logistic regression.

Health literacy is crucial in individuals' ability to make informed health decisions and manage chronic conditions. This study aimed to assess the levels of health literacy and identify socio-demographic factors associated with health literacy among residents aged 15-69 years in Baiyun District, Guangzhou. A cross-sectional survey was conducted among 2630 participants using the China Health Literacy Survey (CHLS) questionnaire between 2019 and 2024.

Facile Synthesis of Non-Noble CuFeCo/C Catalysts with High Stability for ORR in PEMFC.

Proton exchange membrane fuel cells (PEMFCs) have been widely studied as an efficient and environmentally friendly energy conversion technology in recent years. However, the high cost, easy poisoning and complex synthesis methods of noble metal catalysts have hindered their commercialization. Therefore, in this paper, a non-noble metal composite catalyst CuFeCo/C for the oxygen reduction reaction (ORR) was prepared by using a facile liquid-phase reduction method.

Cerebral Perfusion Features in Takayasu Arteritis: Insights From pCASL MRI.

Objective: This study aimed to investigate the cerebral perfusion features in patients with Takayasu arteritis (TA) involving the brain blood supply arteries with pseudo-continuous arterial spin labeling magnetic resonance (pCASL) imaging.

Methods: pCASL-MRI was utilized to measure the cerebral perfusion in the whole brain and various brain regions of healthy individuals and TA patients. The differences in cerebral blood flow (CBF) and arterial transit time (ATT) were compared between TA patients and healthy controls, as well as within TA patients based on the history of cerebral infarction, disease duration, disease activity, and the extent of vascular lesions.

Multifunctional MeHA hydrogel for living materials delivery with enhanced cartilage regeneration.

Particulated juvenile articular cartilage (PJAC) has emerged as a promising living material for articular defect treatment. However, the fragile nature of PJAC hinders its wide clinical application. Here, inspired by the chemical composition and hierarchical structure of natural cartilage, we developed a novel hydrogel carrier system for PJAC delivery.

Berberine as a multi-target therapeutic agent for obesity: from pharmacological mechanisms to clinical evidence.

Obesity, clinically defined by pathological adipose tissue accumulation disrupting metabolic homeostasis, has reached pandemic proportions. The World Obesity Atlas 2024 reports over 1.5 billion projected cases by 2035, highlighting its growing threat among pediatric and adult populations globally.

Exploring the characteristics of retrograde trans-synaptic degeneration from retinal ganglion cells loss in patients with homonymous hemianopia caused by acquired cerebral lesions.

Aim: The existence of retrograde trans-synaptic degeneration (RTD) had been a controversial due to no structural continuity of two neurons in human. The study aimed to detect the macular retinal ganglion cell layer (mRGCL) loss in homonymous hemianopia (HH) patients caused by acquired cerebral lesions using optical coherence tomography (OCT) to explore RTD characteristics.

Methods: A total of 40 HH patients (80 eyes) were enrolled this study.

Lian Qiao Wen Dan Decoction attenuates gastric carcinogenesis by alleviating oxidative phosphorylation dysfunction.

Background: Wen Dan Decoction (WDD) is primarily utilized as an "expectorant" in clinical practice. The combination of WDD and Lian Qiao (LQWDD) exhibits synergistic effects on phlegm resolution, qi regulation, and detoxification. In preliminary clinical practice, we observed that LQWDD effectively alleviates the severity of gastric precancerous lesions.

Salt recovery from a reverse osmosis brine by eutectic freeze crystallization method for waste brine management.

Freeze desalination (FD) is a promising method for the treatment of desalination brines due to its ability to recover clean water and solid salts with a low energy compared to traditional techniques. In this work, eutectic freeze crystallization (EFC) for a reverse osmosis brine was conducted to investigate the feasibility of using EFC for the recovery/removal of different salts such as sodium sulfate (NaSO), magnesium sulfate (MgSO), and sodium chloride (NaCl). An indirect freeze crystallization experiment was conducted on the effect of temperature reduction on multi-component ions of the brine.

Knockdown of MIF-AS1 Inhibits Pain and Inflammation in Lumbar Disc Herniation by Modulating the miR-185-5p/VEGFA Axis.

Study DesignProspective Study.ObjectiveThe objective of this investigation was to explore the abnormal expression of migration inhibitory factor antisense RNA 1 (MIF-AS1) in lumbar disc herniation (LDH) patients and its relationship to the degree of pain and inflammatory response in LDH, as well as the molecular mechanism of its involvement in LDH.MethodsThis study included 50 patients with LDH.

Molecular hydrogen mitigates traumatic brain injury-induced lung injury via NLRP3 inflammasome inhibition.

Introduction: Hydrogen gas has demonstrated significant antioxidant and anti-inflammatory properties, suggesting potential therapeutic benefits in TBI.

Methods: We subjected to controlled cortical impact in mice to construct TBI model. They received an intraperitoneal injection of MCC950, a selective NLRP3 inhibitor, at 10 mg/kg 30 min before TBI.

+RNA Viruses Enhance Plant Drought Tolerance Through Modulation of Phospholipase Dα1 (PLDα1)-Derived Phosphatidic Acid (PA).

Numerous studies suggest that virus infections can improve drought resistance in host plants, but the underlying mechanisms remain unclear. In this study, we used Turnip mosaic virus (TuMV), Potato virus Y (PVY), Tomato bushy stunt virus (TBSV) and the host Nicotiana benthamiana as model systems to investigate these mechanisms. Our findings reveal that the abscisic acid (ABA) signalling pathway is strongly and durably induced by +RNA virus infection and is essential for virus-induced drought tolerance.