Biomimetic lymph node-like scaffolds for optimized CAR-T cell expansion and potentiated antitumor efficacy.

Chimeric antigen receptor T cell (CAR-T) therapy has shown remarkable promise in treating hematological malignancies. However, the expansion of CAR-T cells is time-consuming, potentially impairing CAR-T cell function. Physiologically, T cell activation and proliferation occur within the lymph node (LN) paracortex, a dynamic environment structured by a three-dimensional (3D) reticular network (RN) that promotes cell migration and mediator delivery.

Alteration in conformation and emulsibility of lactoferrin-low methoxyl pectin complexes induced by non-covalent interactions with rosmarinic acid.

Protein-polysaccharide-phenolic acids ternary complexes exhibit unique structural and functional properties, yet the impact of phenolic acids on protein-polysaccharide complexes remains underexplored. This study investigates the non-covalently regulatory effect of rosmarinic acid (RA) on lactoferrin (LF)-low methoxyl pectin (LMP) complexes under various pH conditions. The alterations in particle size and zeta potential are pH-dependent and multi-spectral analysis indicates that the addition of RA leads to a more ordered protein structure.

Enhanced thermal stability of lactoferrin through electrostatic complexation with low-methoxyl pectin.

Lactoferrin (LF) possesses diverse bioactivities, but its application in food systems is limited due to environmental sensitivity and heat-induced denaturation. To improve its thermal stability, electrostatic complexes of LF with low-methoxyl pectin (LMP) were prepared, and their heat resistance was systematically investigated. Based on titration curves, soluble LF-LMP complexes at various mass ratios of 6:1, 4:1, and 2:1 (pH 6 and 7) were created.

The Red Blood Cell Distribution Width-to-Albumin Ratio's Role in Parkinson's Disease: A Cross-Sectional Study.

: The red blood cell distribution width-to-albumin ratio (RAR) serves as an indicator of systemic inflammation and nutritional status. The precise relationship between the RAR and Parkinson's disease (PD) prevalence remains unclear. : This study examines the association between the RAR and PD in U.

Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Alleviates the Defective Epididymis and Sperm Function in Lipopolysaccharide (LPS) Induced Acute Mouse Epididymitis.

This study investigated the protective effects of pituitary adenylate cyclase-activating polypeptide (PACAP) against lipopolysaccharide (LPS)-induced acute epididymitis in mice, with a particular emphasis on its antioxidant and anti-inflammatory mechanisms within the epididymal microenvironment responsible for sperm maturation. Acute epididymitis was triggered by injecting LPS intraperitoneally, with concurrent PACAP administration. Epididymal tissues were collected for histological, immunofluorescence, RT-PCR, and in vitro fertilization analyses.

Effects of ferrous ions partitioning at the oil-water interface on the oxidative stability of Antarctic krill (Euphausia superba) oil: Reverse micelles alter the physical location of ferrous ions.

This study investigates the role of ferrous ion partitioning in promoting lipid oxidation in Antarctic krill oil (AKO) containing reverse micelles. Cryogenic transmission electron microscopy and small-angle X-ray scattering confirmed the presence of reverse micelles in AKO. AKO with ferrous sulfate (FeSO, water-soluble iron) and ferrous fumarate (CHFeO, oil-soluble iron) were stored at 60 °C.

MOTS-c-modified functional self-assembly peptide hydrogels enhance the activity of nucleus pulposus-derived mesenchymal stem cells of intervertebral disc degeneration.

Unlabelled: Intervertebral disc degeneration (IDD) is characterized by oxidative-stress driven progressive apoptosis and senescence of nucleus pulposus mesenchymal stem cells (NP-MSCs). MOTS-c, a 16-amino acid peptide encoded by the mitochondrial 12S rRNA open reading frame, has emerged as a key regulator of cellular metabolism, oxidative stress, and senescence. This study investigated the therapeutic potential of MOTS-c in countering tert-butyl hydroperoxide (TBHP)-induced oxidative damage in NP-MSCs, and we developed a novel biomaterial strategy for IDD treatment.

Addressing the inhibition of Au-S bonding on glucose oxidase-like activity: BSA@Au/MoS-COOH for tandem enzyme-based colorimetric detection of glucose.

Colorimetric/surface-enhanced Raman scattering (SERS) dual-mode detection offers richer detection information and higher accuracy. However, achieving "non-enzymatic" one-step catalytic colorimetric/SERS glucose detection using molybdenum disulfide (MoS)-based nanozymes remains challenging. Here, we propose a tandem enzyme system, BAMC, composed of bovine serum albumin-modified gold nanoparticles (BSA@Au) integrated with carboxylated molybdenum disulfide (MoS-COOH).

CMTM3 Promotes Colitis-associated Carcinogenesis via CLTC Stabilization and Modulation of VE-cadherin.

Background & Aims: Inflammatory bowel disease leads to increased risk of developing colitis-associated colon cancer (CAC). CMTM3 has a higher methylation level in colon cancer, and accumulating evidence suggests that chemokine-like factor-like MARVEL transmembrane domain-containing member 3 (CMTM3) participates in inflammation and cancer development.

Methods: We explored the signs of azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced CAC in wild-type (WT) and Cmtm3 deficiency (Cmtm3) mice.

Expression Patterns and Functional Implications of CMTM2 in Human Testis and Sperm.

The CMTM family plays pivotal roles in various physiological and pathological processes, including those associated with the male reproductive system. Among them, CMTM2 exhibits specific expression in testicular tissue; however, its relationship with spermatogenesis, sperm function, and the underlying molecular mechanisms remains unclear. This study systematically investigated the localization of CMTM2 in human testis and sperm, as well as its correlation with spermatogenesis and sperm quality.

Biomaterial-based strategies: a new era in spinal cord injury treatment.

Enhancing neurological recovery and improving the prognosis of spinal cord injury have gained research attention recently. Spinal cord injury is associated with a complex molecular and cellular microenvironment. This complexity has prompted researchers to elucidate the underlying pathophysiological mechanisms and changes and to identify effective treatment strategies.

Network pharmacology and experimental verification in vivo reveal the mechanism of Zhushao Granules against ulcerative colitis.

Background: Zhushao Granules (ZSG) had exhibited beneficial effects in the treatment of ulcerative colitis (UC) as an effective herbal prescription in Traditional Chinese Medicine. However, the underlying anti-inflammatory mechanism of ZSG remains unclear. This study aimed to decipher the mechanism of ZSG against UC combining network pharmacology and animal-based experiments.

The Systemic Immune-Inflammation Index and the Risk of Parkinson's Disease in the U.S.: A Cross-Sectional Study.

: Inflammation is reportedly related to Parkinson's disease (PD). However, the relationship between the systemic immune-inflammation index (SII) and PD remains unexplored. This study aimed to explore the potential relationship between the SII and PD.

Distribution of different forms of metal ions in Antarctic krill (Euphausia superba) oil: A mechanism of their pro-oxidant effects relating to association colloids.

Due to the fact that association colloids were formed in krill oil, the oxidation mechanism of krill oil was more complicated. In this study, water-soluble ferrous sulfate (Fe(SO)), oil-soluble ferrous fumarate (CHFeO) and insoluble ferric oxide (FeO) were added to krill oil and stored at 60 °C for accelerated oxidation. Peroxide value, thiobarbituric acid reactive substances and aldehyde content showed that Fe(SO) had a stronger pro-oxidative effect.

Non-invasive vagus nerve stimulation in anti-inflammatory therapy: mechanistic insights and future perspectives.

Non-invasive vagus nerve stimulation (VNS) represents a transformative approach for managing a broad spectrum of inflammatory and autoimmune conditions, including rheumatoid arthritis and inflammatory bowel disease. This comprehensive review delineates the mechanisms underlying VNS, emphasizing the cholinergic anti-inflammatory pathway, and explores interactions within the neuro-immune and vagus-gut axes based on both clinical outcomes and pre-clinical models. Clinical applications have confirmed the efficacy of VNS in managing specific autoimmune diseases, such as rheumatoid arthritis, and chronic inflammatory conditions like inflammatory bowel disease, showcasing the variability in stimulation parameters and patient responses.

Molecular mechanisms and behavioral relevance underlying neural correlates of childhood neglect.

Background: Childhood neglect is associated with brain changes, yet the molecular mechanisms and behavioral relevance underlying such associations remain elusive.

Methods: We calculated fractional amplitude of low-frequency fluctuations (fALFF) using resting-state functional MRI and tested their correlation with childhood neglect across a large sample of 510 healthy young adults. Then, we investigated the spatial relationships of the identified neural correlates of childhood neglect with gene expression, neurotransmitter, and behavioral domain atlases.

Physicochemical Properties, Stability, and Functionality of Non-Covalent Ternary Complexes Fabricated with Pea Protein, Hyaluronic Acid and Chlorogenic Acid.

The aim of this study was to prepare and characterize stable non-covalent ternary complexes based on pea protein (PP, 0.5%), hyaluronic acid (HA, 0.125%), and chlorogenic acid (CA, 0~0.

Trace topological doping strategy and deep learning to reveal high-rate sodium storage regulation of barium-doped NaV(PO).

The urgent development of sodium ion batteries has stimulated the rapid innovation of sodium super ionic conductor-type NaV(PO) materials with high energy density and ultra-high charge/discharge rates, where the bottlenecks are the activation of multi-electron reactions and the utilization of the third sodium ion. Herein, we design a trace topological doping strategy to introduce barium ions into crystal domains of NaV(PO) to partially replace vanadium sites. Deep learning demonstrates that the violation of the inversion symmetry of vanadium by barium substitution can improve the structural stability and change the charge density distribution of vanadium, resulting in the re-distribution of surface electrons and supplying more possible migration paths for sodium ions.

Dess-Martin oxidation of hydroxypropyl and hydroxyethyl cellulose, and exploration of their polysaccharide/polypeptide hydrogels.

Oxidation of polysaccharides can provide biomaterials with aldehyde and ketone functional groups, which are particularly useful in biomedical applications, like drug delivery, tissue adhesion and hydrogel preparation. However, despite their potential, only a few such methods have been reported, and achieving selective, quantitative oxidation of polysaccharides remains challenging. Herein we report utilization of a mild oxidant, Dess-Martin periodinane, for the chemoselective oxidation of hydroxypropyl cellulose (HPC) and hydroxyethyl cellulose (HEC).

CMTM6 promotes hepatocellular carcinoma progression through stabilizing β-catenin.

CMTM6, a regulator of PD-L1 stability, has been implicated in the development of various cancers. However, the expression and role of CMTM6 in hepatocellular carcinoma (HCC) remains controversial. Our study revealed a negative correlation between CMTM6 expression and HCC prognosis through bioinformatics analysis and immunofluorescence staining.