A rapid imaging-based screen for induced-proximity degraders identifies a potent degrader of oncoprotein SKP2.

Targeted protein degraders hold potential as therapeutic agents to target conventionally 'undruggable' proteins. Here, we develop a high-throughput screen, DEath FUSion Escaper (DEFUSE), to identify small-molecule protein degraders. By conjugating the protein of interest to a fast-acting triggerable death protein, this approach translates target protein degradation into a cell survival phenotype to illustrate the presence of degraders.

Value of temporal muscle thickness in predicting outcomes of stroke: a systematic review and meta-analysis.

Objective: This systematic review examined if temporal muscle thickness (TMT) as a surrogate marker of sarcopenia was a predictor of outcomes after stroke.

Methods: We explored the PubMed, Embase, Scopus, and Web of Science databases till 18th January 2025 for studies reporting stroke prognosis based on baseline TMT. Pooled analyses examined associations with post-stroke mortality, functional outcomes, and dysphagia.

Ultra-rapid start-up biological nitrification for nutrient recovery from source-separated urine.

Biological nitrification presents a sustainable approach for urine resource recovery. However, high salinity and ammonium concentrations in urine inhibit or even damage microorganisms, causing delayed start-up and unstable. This study first introduces betaine (150 mg·L⁻¹) to enhance urine nitrification by improving microbial salt tolerance and metabolic.

ED and ED of hypobaric ropivacaine during unilateral spinal anesthesia in older patients undergoing hip replacement surgery.

Objective: The spinal block was limited to the operative side during unilateral spinal anesthesia, which has less physiological interference and fewer complications for the patient. The optimal dose of ropivacaine for unilateral spinal anesthesia is still unclear. The aim of this trial was to investigate the ED and ED of hypobaric ropivacaine during unilateral spinal anesthesia in older patients undergoing hip arthroplasty.

Electrocatalytic CO Reduction to Chemicals and Fuels: From Single-Atom to Dual-Atom.

In recent years, single-atom catalysts (SACs) have emerged as a prominent research focus in electrochemical CO reduction reactions (CORR), owing to their exceptional atomic utilization efficiency and superior catalytic performance. Nevertheless, their practical implementation may be constrained by inherently low metal loading and the presence of linear relationships imposed by their structurally simplistic active sites. Atomic-level engineering of active sites represents a transformative strategy to overcome the intrinsic limitations of SACs.

Comparison of postoperative analgesic effects of ultrasound-guided intercostal nerve block and transversus abdominis plane block in patients undergoing laparoscopic cholecystectomy: randomized clinical trial.

Background: The aim of this study was to compare the postoperative analgesic effects of ultrasound-guided intercostal nerve block and transversus abdominis plane block in patients undergoing laparoscopic cholecystectomy.

Methods: Patients undergoing laparoscopic cholecystectomy for chronic cholecystitis with gallstones were randomly allocated to ultrasound-guided T7-11 intercostal nerve block or subcostal transversus abdominis plane block (both with 40 ml 0.3% ropivacaine).

Effects of Dyclonine Mucilage and Compound Lidocaine Cream as Tracheal Catheter Lubricant on Postoperative Pharyngeal Complications After General Anesthesia.

Background: The aim of this study was to observe the effects of dyclonine mucilage and compound lidocaine cream on postoperative pharyngeal complications after general anaesthesia.

Methods: Ninety patients were randomly divided into a dyclonine mucilage group (D group), a compound lidocaine cream group (L group) and a normal saline group (NS group). The primary outcomes were the incidence and severity of postoperative sore throat (POST).

Effect of dexmedetomidine on the median effective concentration of ropivacaine for postoperative analgesia in transversus abdominis plane block: an up-down sequential allocation study.

Background: This study aimed to observe the effect of dexmedetomidine on the median effective concentration (EC) of ropivacaine for postoperative analgesia in ultrasound-guided transversus abdominis plane block.

Methods: Patients undergoing elective laparoscopic cholecystectomy were randomly divided into the RD group and the R group. In the RD group, 40 mL of ropivacaine with 1 μg/kg dexmedetomidine was injected into the transverse abdominis plane, while subjects in the R group received equal volumes of ropivacaine with normal saline.

Ultraviolet Photodissociation Mass Spectrometry Captures the Acyl Chain Length-Dependent Conformation Dynamics of Acyl Carrier Protein.

Capturing the acyl chain dependent conformation dynamics of acyl carrier protein (ACP) is critical for understanding the molecular mechanism of acyl chain stabilization and elongation, providing structural insights for ACP evolution. Herein, we utilize native mass spectrometry (nMS) and 193 nm ultraviolet photodissociation (UVPD) to systematically interrogate the structural details in activation and interactions of ACP with C4-C18 acyl chains. The unstable acyl-ACP intermediates can be isolated and subjected to high-sensitivity UVPD analysis individually without matrix interference.

Fabrication of a CuS-cocatalyst-supported g-CN nanosheet composite photocatalyst with improved performance in the photocatalytic reduction of CO.

Carbon dioxide (CO) is not only a greenhouse gas but also an abundant carbon resource. By using solar energy to reduce CO into high-value hydrocarbons photocatalysis, we can mitigate the greenhouse effect and enable energy recycling. In this paper, a two-step calcination process was employed to thermally exfoliate graphite-phase carbon nitride (g-CN) into ultrathin nanosheets, after which the CuS co-catalyst was loaded onto the g-CN surface using a one-step hydrothermal method.

Gold Cluster-Based Profiling of Early Stage Acute Kidney Injury through Second Near-Infrared Fluorescence Imaging and Mass Spectrometry Imaging.

Acute kidney injury (AKI) poses a major challenge due to the rapid deterioration of renal function, for which the optimal intervention timing has already passed until clinical diagnostics sense abnormalities. Meanwhile, the lack of means to visually understand early-stage AKI at the molecular level hinders the development of interventive strategies. Herein, we developed a high-orthogonal dual-mode imaging strategy integrating second near-infrared (NIR-II) fluorescence imaging and mass spectrometry imaging (MSI) based on gold clusters for profiling early-stage AKI.

Diagnostic accuracy of rapid shallow breathing index based on diaphragm ultrasound predicting successful weaning from mechanical ventilation: A systematic review and meta-analysis.

Objectives: This meta-analysis aimed to evaluate the effectiveness of the rapid shallow breathing index based on diaphragm ultrasound, specifically the diaphragmatic excursion-rapid shallow breathing index (DE-RSBI) and the diaphragmatic thickness fraction- rapid shallow breathing index (DTF-RSBI), in predicting successful weaning from mechanical ventilation.

Method: Two researchers independently searched four databases, PubMed, Embase, Cochrane Library and Web of Science, from their inception until 2 November 2024, and conducted literature screening and data extraction. The QUADAS-2 (Quality Assessment of Diagnostic Accuracy Studies 2) was employed to evaluate the methodological quality of the included studies.

In-Cell Mass Spectrometry and Ultraviolet Photodissociation Navigates the Intracellular Protein Heterogeneity.

Directly probing the heterogeneous conformations of intracellular proteins within their native cellular environment remains a significant challenge in mass spectrometry (MS). Here, we establish an in-cell MS and ultraviolet photodissociation (UVPD) strategy that directly ejects proteins from living cells into a mass spectrometer, followed by 193 nm UVPD for structural analysis. Applying this approach to calmodulin (CaM), we reveal that it adopts more extended conformations within living cells compared with purified samples , highlighting the unique influence of intracellular environments on protein folding.

Spatial multi-omics characterizes GPR35-relevant lipid metabolism signatures across liver zonation in MASLD.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a metabolic disease that can progress to metabolic dysfunction-associated steatohepatitis (MASH), cirrhosis, and cancer. The zonal distribution of biomolecules in the liver is implicated in mediating the disease progression. Recently, G-protein-coupled receptor 35 (GPR35) has been highlighted to play a role in MASLD, but the precise mechanism is not fully understood, particularly, in a liver-zonal manner.

[Vacuum ultraviolet laser dissociation and proteomic analysis of halogenated peptides].

Chemical modifications are widely used in research fields such as quantitative proteomics and interaction analyses. Chemical-modification targets can be roughly divided into four categories, including those that integrate isotope labels for quantification purposes, probe the structures of proteins through covalent labeling or cross-linking, incorporate labels to improve the ionization or dissociation of characteristic peptides in complex mixtures, and affinity-enrich various poorly abundant protein translational modifications (PTMs). A chemical modification reaction needs to be simple and efficient for use in proteomics analysis, and should be performed without any complicated process for preparing the labeling reagent.

Effect of Dexmedetomidine on the ED and ED of Sufentanil in Patient-Controlled Intravenous Analgesia After Cesarean Section: A Randomized, Controlled, Double-Blind Trial.

Purpose: To determine the effect of dexmedetomidine on the ED and ED of sufentanil in patient-controlled intravenous analgesia (PCIA) after cesarean section.

Patients And Methods: Parturients who underwent elective cesarean section (n = 80) were randomly assigned to either the sufentanil group (S group) or the dexmedetomidine-sufentanil combination group (DS group). Patients in the S group received a combination of sufentanil, 5 mg of tropisetron, and saline, whereas patients in the DS group were administered 1.

Randomized controlled trial on effect of different routes of dexmedetomidine on Haemodynamics in patients undergoing saphenectomy under epidural anaesthesia.

The effect of epidural infusion of dexmedetomidine on haemodynamics is unclear. This study aimed to explore the effects of epidural or intravenous infusion of dexmedetomidine on haemodynamics during lower extremity varicose veins surgery (saphenectomy) under epidural anaesthesia. Ninety patients were randomly allocated to three groups: ED group (epidural: 0.

Postoperative analgesic effect of intravenous coinjection of lidocaine and dexmedetomidine in gynaecological surgery: a systematic review and meta-analysis.

Objectives: Hysterectomy or myomectomy is a common gynaecological procedure that results in moderate to severe acute postoperative pain, which can cause many adverse effects. This study aimed to compare the postoperative analgesic efficacy, opioid consumption, quality of postoperative recovery (QOR) and adverse reactions of intravenous coinjection of lidocaine and dexmedetomidine versus lidocaine or dexmedetomidine alone in gynaecological surgery.

Design: Systematic review and meta-analysis was performed.

Effect of different doses of esketamine on the median effective concentration of propofol for inhibiting body movement during hysteroscopy.

The objective of this study is to investigate the effects of various doses of esketamine on the median effective concentration (EC) of propofol required for inhibiting body movement during hysteroscopy. Additionally, this research aims to explore the pharmacodynamic interactions between esketamine and propofol. Prospective, double-blind, up-down sequential allocation study.

Evaluating heavy metals-related risk in staple crops and making financing strategy for corresponding soil remediation across China.

China's staple crops face heavy metal (HMs) contamination, a widespread issue lacking a national assessment. We used machine learning (ML) to assess risks of 8 HMs in rice, wheat, and maize, and estimated a financing strategy for soil remediation via linear optimization and computable general equilibrium (CGE). The accumulation of HMs in crops depends on Soil-HMs, climate, soil properties, and crop types.

Protective effects of fructose-1,6-bisphosphate postconditioning on myocardial ischaemia-reperfusion injury in patients undergoing valve replacement: a randomized, double-blind, placebo-controlled clinical trial.

Objectives: Pharmacological postconditioning can protect against myocardial ischaemia-reperfusion injury during cardiac surgery with extracorporeal circulation. The aim of this study was to observe the protective effects of fructose-1,6-bisphosphate (FDP) postconditioning on myocardial ischaemia-reperfusion injury in patients undergoing cardiac valve replacement with extracorporeal circulation.

Methods: Patients undergoing elective mitral valve replacement and/or aortic valve replacement were divided into normal saline postconditioning group (NS group) and FDP postconditioning group (FDP group).

The optimal dose of dexmedetomidine as a 0.59% ropivacaine adjuvant for epidural anesthesia in great saphenous varicose vein surgery, based on hemodynamics and anesthesia efficacy: a randomized, controlled, double-blind clinical trial.

Objective: This study aimed to explore the optimal dose of dexmedetomidine as a 0.59% ropivacaine adjuvant for epidural anesthesia on perioperative hemodynamics and anesthesia efficacy in patients undergoing great saphenous varicose vein surgery.

Methods: A total of 90 patients were randomly divided into three groups: 0.

[Applications of native mass spectrometry and ultraviolet photodissociation in protein structure and interaction analysis].

Dynamic changes in the structures and interactions of proteins are closely correlated with their biological functions. However, the precise detection and analysis of these molecules are challenging. Native mass spectrometry (nMS) introduces proteins or protein complexes into the gas phase by electrospray ionization, and then performs MS analysis under near-physiological conditions that preserve the folded state of proteins and their complexes in solution.