Selection and characterization of novel DNA aptamers targeting colorectal cancer cells with malignant potential.

Colorectal cancer (CRC) is associated with high morbidity and mortality rates worldwide. Therefore, early diagnosis and treatment are of great significance for improving the survival rate of CRC patients. Herein, we performed subtractive cell-SELEX using two cell lines (LS174T and CL187) with the same genetic background but different malignant potentials for the target and negative cells.

A novel technique for posterior internal fixation of acetabular fractures.

Purpose: To evaluate the surgical technique and clinical outcomes of the Universal Self-locking Anatomic Plate for Acetabulum (USAPA).

Methods: A retrospective analysis of 155 patients with complete follow-up data from December 2014 to December 2020 was conducted. The study included 119 males and 36 females, aged 18–82 (mean: 40.

Effects of α-amylase supplementation on production performance, blood metabolites, nutrient digestibility, and rumen fermentation parameters of Holstein dairy cows in late lactation.

Current research on dairy cows primarily focuses on peak lactation, with limited exploration of late lactation. This study investigated the effects of α-amylase supplementation on production performance, blood metabolites, nutrient digestibility, and rumen fermentation in late-lactation Holstein cows. Thirty cows (average milk yield: 37.

Experimental study on mechanical response characteristics of gassy sand under constant shear stress paths.

The stress state changes in shallow gassy sand strata under anthropogenic disturbances can be simplified as failure problems of gassy sand under constant shear stress drained (or undrained) stress paths. However, related research remains relatively limited. To investigate the mechanical properties of gassy sand, this study utilized a high-pressure gas dissolution saturator and employed a CO-saturated aqueous solution degassing method to prepare high-saturation gassy sand specimens.

Unraveling α-synuclein and amylin co-aggregation: pathological insights and biomarker development for Parkinson's disease.

Patients with diabetes have a higher morbidity in Parkinson's disease (PD) than others, but the mechanism underlying this link remains controversial. The co-aggregation of α-synuclein (α-syn) and amylin has been hypothesized as a key contributor. Molecular interaction analysis and co-immunoprecipitation were conducted to assess the feasibility of co-aggregation.

Translation and psychometric evaluation of the Chinese version of the arterial puncture self-efficacy scale.

Background: Arterial blood gases (ABG) analysis is an indispensable yet difficult-to-perform skill in nursing practice. In the acquisition of this skill, nurses' self-efficacy plays a crucial role. Nevertheless, there is presently a shortage of a dependable tool for assessing the self-efficacy of Chinese nurses when it comes to performing arterial punctures for ABG analysis.

Proteomics reveals crosstalk between calcium disturbance and multiple stresses facilitating lethal ventricular arrhythmias-sudden cardiac death under the co-morbidity of acute myocardial ischemia and type II diabetes mellitus.

Aims: Diabetes mellitus (DM) often co-exists with myocardial ischemia (MI). Whether DM increases sudden cardiac death (SCD) susceptibility in early MI remains unclear, and the underlying mechanism needs to be further explored.

Materials And Methods: We induced type II DM in mice to produce a co-morbidity model of DM and MI.

Clinically Accurate Diagnosis of Alzheimer's Disease via Single-Molecule Bioelectronic Label-Free Profiling of Multiple Blood Extracellular Vesicle Biomarkers.

Alzheimer's disease (AD) is a progressive neurodegenerative disorder with no cure, making early diagnosis critical for mitigating its impact. Blood extracellular vesicles (EVs) hold promises as biomarkers for AD diagnosis, but current detection technologies lack the sensitivity and multiplexing capabilities needed for efficient diagnosis. Here, a novel label-free bioelectronic platform is presented based on an organic electrochemical transistor (OECT) integrated with a microelectrode array (MEA) for ultrasensitive detection of AD biomarkers in blood EVs, including amyloid-β (Aβ and Aβ), total tau (t-tau), and phosphorylated tau (p-tau).

Clinical indicators associated with tuberculous meningitis using multiple correspondence analysis.

Objectives: Tuberculous meningitis (TBM), the most severe extrapulmonary manifestation of Mycobacterium tuberculosis (Mtb) infection, contributes to 5-10 % of global tuberculosis-related mortality. This study aimed to delineate clinical indicators predictive of microbiologically confirmed TBM to facilitate early diagnosis in resource-limited settings.

Methods: A retrospective cohort analysis was conducted on 146 TBM cases admitted to Shenzhen Third People's Hospital (2018-2020), undergoing cerebrospinal fluid (CSF) culture and Xpert.

Rethinking the Prognostic Role of Necrosis in Soft-Tissue Sarcoma: Multidisciplinary Insights from the Sarcoma Academy.

Soft-tissue sarcomas (STSs) represent a heterogeneous group of malignancies with widely varying treatment responses and biological behaviors. While spontaneous necrosis (present at diagnosis) is recognized in established sarcoma grading systems, the prognostic significance of therapy-induced necrosis remains uncertain. Inconsistent definitions, methodological variability, and clinical confounders further complicate the interpretation of necrosis as an independent prognostic marker.

Supplementation of lentinan improves lactation performance by altering ruminal microorganisms and metabolites in dairy cows.

Lentinan (LNT) is a bioactive polysaccharide that has been proven to improve the production performance of animals. The objective of this study was to investigate the effects of LNT on lactation performance and rumen fermentation in dairy cows. Through random block design, 80 Holstein cows were randomly assigned to four treatments (CON, control group, fed a basal diet; and 5LNT, 10LNT, 15LNT groups, fed a basal diet with 5, 10, 15 g/d LNT, respectively) according to the days in milk (57 ± 21.

Integrating phosphoproteome and proteome to reveal key processes through which apocynin protects from lethal ventricular arrhythmias-sudden cardiac death in early myocardial ischemia.

The specific role of NADPH oxidases (NOX)-dependent reactive oxygen species (X-ROS) in the development of lethal ventricular arrhythmias (LVA)-sudden cardiac death (SCD) during the early stage of myocardial ischemia (MI) remains poorly understood. Herein, we investigated the impact of X-ROS on LVA-SCD in early MI. The mouse model of MI-induced LVA-SCD and the apocynin (a NOX inhibitor)-pretreated control groups were established.

Differences in DHA bioaccessibility and fat digestion of DHA-enriched milks: An in vitro infant digestion model.

Docosahexaenoic acid (DHA) is essential for infant neurodevelopment, and milk is an effective vehicle for delivering DHA to infants. However, it remains unclear how different production and processing methods affect the bioavailability of milk study evaluated the bioaccessibility of DHA in different DHA-enriched milks using a simulated infant digestive model. The results showed that naturally DHA-enriched milk and homogenized naturally DHA-enriched milk exhibited higher DHA bioaccessibility (52.

Furosemide sodium enhanced AQP3 expression by inhibiting NKCC1 leading to improved vitrification freezing rates of mouse and bovine blastocysts.

Furosemide sodium, a loop diuretic that inhibits the Na-K-2Cl cotransporter (NKCC1), is commonly used in clinical treatment of edema. In this study, we investigated whether furosemide sodium could improve the survival and quality of vitrified mouse and bovine blastocysts by promoting the expulsion of blastocoelic fluid, thus enhancing cryoprotectant penetration and reducing ice crystal formation. Mouse and bovine blastocysts were vitrified using equilibration solutions (ES) and vitrification solutions (VS) with or without furosemide sodium, then thawed for survival and quality assessment.

Effects of dietary oregano essential oil on digestive tissue structure and function, antioxidant and immune responses and gut microbiota of red swamp crayfish (Procambarus clarkii).

Modifying the diet is an effective strategy to enhance the production and health of farmed red swamp crayfish (Procambarus clarkii). In this study the effects of adding dietary oregano essential oil (OEO) at 0, 100, 300, and 500 mg/kg concentrations were investigated in red swamp crayfish after a 3-week feeding trial. Histology showed no negative impacts on the structural integrity of the hepatopancreas and intestine.

Immunotherapy benefits PD‑L1‑positive gastric‑type endocervical adenocarcinoma: A multicenter, retrospective study.

Gastric-type endocervical adenocarcinoma (GEA) usually exhibits notable aggressiveness and resistance to current therapies. A high expression of programmed death-ligand 1 (PD-L1) was previous reported in GEA and indicated it might benefit from immunotherapy targeting programmed cell death protein 1 (PD-1)/PD-L1. In the present study, the efficacy of immunotherapy in a panel of patients with GEA was explored, aiming to provide the first-hand evidence on this topic.

Supplementation with Lentinan Improves the Colostrum Quality of Holstein Dairy Cows and the Immunity and Antioxidant Capacity of Newborn Calves.

The aim of this study was to evaluate the effects of lentinan (LNT) on the quality of colostrum produced by perinatal dairy cows and the health status of their newborn calves. A total of 40 expectant Holstein cows, matched for parity and expected date of delivery, were selected and randomly divided into four groups: a control group fed a total mixed diet (TMR); a low LNT group (10 g/d, LLNT); a medium LNT group (20 g/d, MLNT); and a high LNT group (40 g/d, HLNT). The study commenced 21 days prior to parturition and continued for three weeks.

Tunable ultra-broadband plasmonic terahertz absorber based on ultrathin phase-change metamaterials.

The paper proposes an ultrathin and tunable ultrawideband plasmonic terahertz absorber based on vanadium dioxide (VO) phase transition metamaterials, with a thickness of only 5.98 micrometers, to address the current issues of insufficient frequency tunability and limited bandwidth coverage in terahertz absorbers. The absorber features a multilayer composite structure consisting of a bottom Au metal layer, a SiO dielectric layer, a VO layer, an upper SiO layer, and a patterned VO layer on the surface.

Identification of Hydroxylated Chlorinated Paraffins in Human Serum and Their Potential Metabolic Pathways.

Short- and medium-chain chlorinated paraffins (SCCPs and MCCPs) are frequently detected in humans. However, information regarding their metabolites is still very limited. Herein, target analysis and halogenation-guided nontarget and suspect screening were conducted on serum samples using UHPLC-Orbitrap-HRMS.

Visible-Light-Driven Catalytic Dehalogenation of Trichloroacetic Acid and α-Halocarbonyl Compounds: Multiple Roles of Copper.

Herein, we report the reaction development and mechanistic studies of visible-light-driven Cu-catalyzed dechlorination of trichloroacetic acid for the highly selective formation of monochloroacetic acid. Visible-light-driven transition metal catalysis via an inner-sphere pathway features the dual roles of transition metal species in photoexcitation and substrate activation steps, and a detailed mechanistic understanding of their roles is crucial for the further development of light-driven catalysis. This catalytic method, which features environmentally desired ascorbic acid as the hydrogen atom source and water/ethanol as the solvent, can be further applied to the dehalogenation of a variety of halocarboxylic acids and amides.

Communicating COVID-19 vaccine information to Chinese communities in the UK: a qualitative study of their knowledge, information sources and trust.

Introduction: In late 2020, the COVID-19 vaccine acceptance rates of Chinese people living in the UK were estimated between 52% and 57%, significantly lower than that of the general population (76%). This disparity formed a primary motivation for this study which explored Chinese communities' overall understanding of and attitude towards the COVID-19 vaccines, the sources of information about the vaccines and levels of (dis)trust in these sources.

Methods: 29 focus groups with 154 participants from UK-based Chinese communities of varied sociodemographic backgrounds were conducted between March and November 2021.

The efficacy of anti-angiogenic drugs in gastric-type endocervical adenocarcinoma: A retrospective study.

Objectives: Gastric-type endocervical adenocarcinoma (GEA) is a rare malignant tumor that is not associated with high-risk HPV infection, known for its high invasiveness and resistance to current treatments. This study assessed the effectiveness of anti-angiogenic regimens in real-world GEA patients.

Methods: Patients with GEA were enrolled between February 2012 and March 2023, and their clinicopathological characteristics were collected from their medical records.

Real-world data from patients with gastric-type mucinous carcinoma of the cervix: a multicenter, retrospective study.

Background: We herein retrospectively analyzed the clinicopathologic features from a large cohort of GAS patients to provide real-world evidence for optimizing the diagnosis and treatment.

Research Design And Methods: One hundred and fifty-seven GAS patients from three hospitals were recruited for analysis. We extracted clinical and pathologic information from patient medical records and performed regular follow-up.

The Contribution and Perspectives of Proteomics to Epithelial Ovarian Cancer.

Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy which mainly consists of serous, mucinous, clear cell, and endometrioid subtypes. Due to the lack of classic symptoms at an early stage, EOC usually presented as advanced tumors with local and/or distant metastasis. Although a large portion of EOC was initially platinum-sensitive, most patients would acquire resistance to common chemotherapeutic agents.