Metabolomics and Transcriptomics Reveal Age-Dependent Development of Meat Quality Traits in Jingyuan Chicken.

In this study, we investigated the dynamic changes in muscle lipid content (MLC) deposition in the breast muscle of Jingyuan chickens at different stages (42-, 126-, and 180- days old) using metabolomics. The pH a*, and L* were significantly increased in the breast muscle of 126-day-old chickens, and MLC and b* were significantly increased in the breast muscle of 180-day-old chickens. A total of 4643 differentially expressed metabolites (DEMs) were identified, of which 10 decreased and 29 increased with age.

APOE mediates the coupling of myogenesis and lipid metabolism in skeletal muscle: decoding intercellular crosstalk via a cell co-culture model.

The growth and lipid accumulation of skeletal muscle directly affects the yield and meat quality characteristics of beef, which is the result of the interaction between skeletal muscle cells (SMCs) and intramuscular adipocytes (IMAs). Therefore, in this study, a co-culture system was used to simulate the in vivo cellular microenvironment, aiming to reveal the interaction patterns between bovine SMCs and IMAs during the proliferation and differentiation stages. The results showed that, compared with SMCs cultured alone, the presence of IMAs in the co-culture system inhibited the expression of proliferation markers such as PCNA, CDK1, CDK2, CCNA2, and CCNE2 in SMCs.

Insertion sequences accelerate genomic convergence of multidrug resistance and hypervirulence in Klebsiella pneumoniae via capsular phase variation.

Background: The convergence of resistance and hypervirulence in Klebsiella pneumoniae represents a significant public health threat, driven by the horizontal transfer of plasmids. Understanding factors affecting plasmid transfer efficiency is essential to elucidate mechanisms behind emergence of these formidable pathogens.

Methods: Hypermucoviscous K.

Leveraging collateral sensitivity to counteract the evolution of bacteriophage resistance in bacteria.

The escalating antibiotic resistance crisis poses a major global health threat. Bacteriophage therapy offers a promising alternative for combating multidrug-resistant infections. However, bacterial resistance to phages remains a significant hurdle.

Vitamin A mediates FABP4 to regulate intramuscular fat production: a new target and strategy for optimizing beef quality.

Beef quality is critically determined by intramuscular fat (IMF) deposition. Retinoic acid (RA), the active metabolite of vitamin A, plays an essential regulatory role in IMF development. To systematically investigate RA-mediated regulation of IMF formation in cattle, we established a concentration gradient of RA supplementation and employed a systematic screening approach to identify the optimal dosage for modulating bovine intramuscular adipocytes (IMAs) proliferation and differentiation.

Unveiling the Mechanisms of Digital Technology in Driving Farmers' Green Production Transformation: Evidence from China's Watermelon and Muskmelon Sector.

Leveraging the transformative potential of digital technologies to support the farmers' green production transformation (FGPT) is a critical catalyst for facilitating the accelerated green transformation and upgrading of agricultural systems, thereby achieving high-quality agricultural development. Using survey data from major watermelon- and muskmelon-producing regions in Henan, Shandong, and Xinjiang, and employing Ordinary Least Squares (OLS), Propensity Score Matching (PSM), and the Logit model, this study examines the mechanisms through which digital technology influences FGPT. The findings reveal that the adoption of digital technology significantly reduces farmers' use of pesticides and fertilizers, with a particularly pronounced effect on pesticide reduction.

Flexible Terahertz Broadband Absorber Based on a Copper Composite Film.

Terahertz absorbers play a crucial role in terahertz detectors, radar stealth, electromagnetic shielding, and other fields. However, the design and fabrication of flexible terahertz broadband absorbers remain a challenge at present. Here, we demonstrated a terahertz broadband absorber based on a copper composite film (CCF) consisting of a copper foam and an organic silica gel doped with FeO powder.

Characterization of a novel phage against multidrug-resistant Klebsiella pneumoniae.

Multidrug-resistant Klebsiella pneumoniae (MDR-KP) poses a significant challenge in global healthcare, underscoring the urgency for innovative therapeutic approaches. Phage therapy emerges as a promising strategy amidst rising antibiotic resistance, emphasizing the crucial need to identify and characterize effective phage resources for clinical use. In this study, we introduce a novel lytic phage, RCIP0100, distinguished by its classification into the Chaoyangvirus genus and Fjlabviridae family based on International Committee on Taxonomy of Viruses (ICTV) criteria due to low genetic similarity to known phage families.

SGK1 promotes the lipid accumulation via regulating the transcriptional activity of FOXO1 in bovine.

Objectives: Serum/glucocorticoid-inducible kinase 1 (SGK1) gene encodes a serine/threonine protein kinase that plays an essential role in cellular stress response and regulation of multiple metabolic processes. However, its role in bovine adipogenesis remains unknown. In this study, we aimed to clarify the role of SGK1 in bovine lipid accumulation and improvement of meat quality.

Regulatory role of exosome-derived miRNAs and other contents in adipogenesis.

Intramuscular fat (IMF) content significantly impacts meat quality. influenced by complex interactions between skeletal muscle cells and adipocytes. Adipogenesis plays a pivotal role in IMF formation.

Data-Driven Engineering of Phages with Tunable Capsule Tropism for Klebsiella pneumoniae.

Klebsiella pneumoniae, a major clinical pathogen known for causing severe infections, is attracting heightened attention due to its escalating antibiotic resistance. Phages are emerging as a promising alternative to antibiotics; however, their specificity to particular hosts often restricts their use. In this study, a collection of 114 phages is obtained and subjected to analysis against 238 clinical K.

Understanding the rapid spread of antimicrobial resistance genes mediated by IS.

Insertion sequences (ISs) promote the transmission of antimicrobial resistance genes (ARGs) across bacterial populations. However, their contributions and dynamics during the transmission of resistance remain unclear. In this study, we selected IS as a representative transposable element to decipher the relationship between ISs and ARGs and to investigate their transfer features and transmission trends.

A case report of cardia carcinoma: Underwent Heller myotomy for Achalasia.

Background: One of the risk factors for esophageal adenocarcinoma is achalasia, an esophageal motility disorder that is typically treated surgically through laparotomy or laparoscopic surgery. The risk factors of gastric cardia cancer are also similar to esophageal adenocarcinoma due to the anatomical location of the gastric cardia close to the esophagus. There is currently no clinical evidence that achalching has a correlation with gastric cardia cancer.

FOXO1 regulates bovine skeletal muscle cells differentiation by targeting MYH3.

The growth and development of bovine skeletal muscle and beef yield is closely intertwined. Our previous research found that forkhead box O1 (FOXO1) plays an important role in the regulation of beef muscle formation, but its specific mechanism is still unknown. In this study, we aimed to clarify the regulatory mechanism of FOXO1 in proliferation and differentiation of bovine skeletal muscle cells (BSMCs).

Tongxinluo capsule in combination with conventional therapies for stable angina pectoris: A meta-analysis and systematic review.

Background: This study aimed to assess the efficacy and safety of Tongxinluo capsule (TXLC) in combination with conventional therapies for treating stable angina pectoris (SAP) through a comprehensive meta-analysis and systematic review.

Methods: We conducted a systematic search of the China National Knowledge Infrastructure, Wanfang, VIP, PubMed, Embase, and CENTRAL databases for randomized controlled trials investigating the use of TXLC as adjuvant therapy for SAP published up to June 2023. The Cochrane Handbook was used to evaluate the risk of bias.

Phylogenomic insights into evolutionary trajectories of multidrug resistant S. pneumoniae CC271 over a period of 14 years in China.

Background: Streptococcus pneumoniae is a gram-positive opportunistic pathogen, and infection risks of S. pneumoniae can be profoundly augmented by its acquired multidrug-resistance (MDR). The rapid development of MDR in S.

Interaction of C/EBPβ with SMAD2 and SMAD4 genes induces the formation of lipid droplets in bovine myoblasts.

As a key component of Transforming growth factor-β (TGF-β) pathway, Smad2 has many crucial roles in a variety of cellular processes, but it cannot bind DNA without complex formation with Smad4. In the present study, the molecular mechanism in the progress of myogenesis underlying transcriptional regulation of SMAD2 and SMAD4 had been clarified. The result showed the inhibition between SMAD2 and SMAD4, which promotes and inhibits bovine myoblast differentiation, respectively.

CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p.

Circular RNAs (CircRNAs) are covalently closed-loop non-coding RNA (ncRNA) molecules present in eukaryotes. Numerous studies have demonstrated that circRNAs are important regulators of bovine fat deposition, but their precise mechanisms remain unclear. Previous transcriptome sequencing studies have indicated that circADAMTS16, a circRNA derived from the a disintegrin-like metalloproteinases with the thrombospondin motif 16 () gene, is high expressed in bovine adipose tissue.

NAD+ Biosynthesis Impairment and Acute Kidney Injury after Major Vascular Surgery.

Acute kidney injury (AKI) is a serious complication after vascular surgery. Reduced synthesis of nicotinamide adenine dinucleotide (NAD+) from tryptophan is associated with an increased risk of AKI in critically ill patients, patients hospitalized with COVID-19, and cardiac surgery patients, and is marked by elevated urinary quinolinate and quinolinate to tryptophan ratios. We measured quinolinate concentrations in vascular surgery patients to determine if impaired NAD+ synthesis was associated with AKI in this patient population.

Identification of key genes in bovine muscle development by co-expression analysis.

Background: Skeletal muscle is not only an important tissue involved in exercise and metabolism, but also an important part of livestock and poultry meat products. Its growth and development determines the output and quality of meat to a certain extent, and has an important impact on the economic benefits of animal husbandry. Skeletal muscle development is a complex regulatory network process, and its molecular mechanism needs to be further studied.