Development and Immunogenic Evaluation of a Recombinant Vesicular Stomatitis Virus Expressing Nipah Virus F and G Glycoproteins.

Nipah virus (NiV) is a highly pathogenic bat-borne zoonotic pathogen that poses a significant threat to human and animal health, with fatality rates exceeding 70% in some outbreaks. Despite its significant public health impact, there are currently no licensed vaccines or specific therapeutics available. Various virological tools-such as reverse genetics systems, replicon particles, VSV-based pseudoviruses, and recombinant Cedar virus chimeras-have been widely used to study the molecular mechanisms of NiV and to support vaccine development.

Te Vacancy Defect Engineering on FeGeTe (001) Basal Planes for Enhanced Oxygen Evolution Reaction: A First-Principles Study.

Photocatalytic water splitting for hydrogen production is an attractive renewable energy technology, but the oxygen evolution reaction (OER) at the anode is severely constrained by a high overpotential. The two-dimensional vdW ferromagnetic material FeGeTe, with its good stability and excellent metallic conductivity, has potential as an electrocatalyst, but its sluggish surface catalytic reactivity limits its large-scale application. In this work, we adapted DFT calculations to introduce surface Te vacancies to boost OER performance of the FeGeTe (001) surface.

Metal-π Interaction-Enhanced Electrochemiluminescence via U-Shaped Schiff Base Luminophores for Ultrasensitive Clindamycin Detection.

The advancement of electrochemiluminescence (ECL) sensing has been increasingly restricted by the lack of rationally designed luminophores that simultaneously support efficient charge transfer, excited-state stability, and catalytic amplification. Herein, we report a Fe(II)-coordinated U-shaped Schiff base luminophore (Fe-PDLILUM) that achieves significantly enhanced ECL via synergistic metal-π interactions and topological rigidification. This luminophore is synthesized through imine-bridged conjugation between isoluminol and 1,10-phenanthroline-2,9-dicarboxaldehyde, forming an extended π-delocalized framework that promotes intramolecular charge transport and suppresses nonradiative decay.

Rapid identification of high-viscosity rice soup quality using neutral desorption extractive electrospray ionization mass spectrometry.

High-viscosity rice soup is not only a popular food item but also a traditional Chinese medicinal remedy. Herein, we developed a high-throughput characterization platform for high-viscosity rice soup analysis using Neutral Desorption Extractive Electrospray Ionization Mass Spectrometry (ND-EESI-MS) with untargeted metabolomics. The chemical fingerprint of high-viscosity rice soup was directly analyzed by ND-EESI-MS, with exogenous (, heavy metals and pesticides) and endogenous pollutants detected qualitative and quantitative tandem mass spectrometry.

Pyruvate Dehydrogenase Kinase 4 Underlies the Metabolic Disorder of Cardiomyocytes in Patients With Hypertrophic Cardiomyopathy From Hypertrophy to Heart Failure.

Background: Hypertrophic cardiomyopathy (HCM), characterized by ventricular hypertrophy and fibrosis, frequently progresses to heart failure. Although metabolic dysregulation is implicated in HCM pathophysiology, the role of PDK4 (pyruvate dehydrogenase kinase 4), a key regulator of cardiac glucose and fatty acid oxidation, in HCM-related heart failure remains unknown.

Methods: Single-nucleus RNA sequencing was performed to analyze gene expression in patients with HCM (n=12), categorized into the following groups: normal, reduced, and heart failure.

Intrahepatic cholestasis of pregnancy.

Intrahepatic cholestasis of pregnancy is the most common pregnancy-related liver disease, manifesting typically during the third trimester of pregnancy with pruritus and elevated serum bile acids. This condition is associated with increased fetal morbidity and mortality, and its pathogenesis is still incompletely understood, but is most likely multifactorial, involving ethnicity, genetics, hormones and environmental factors. Available evidence covering the pathophysiology of both maternal and fetal manifestations, and potential new areas of interest such as microbiota and the environment, have been reviewed, as well as available biomarkers that can be used particularly with regard to genetics, multiomics and the possible use of machine learning algorithms to predict intrahepatic cholestasis of pregnancy.

Graphene-Based Nanomaterials in Photodynamic Therapy: Synthesis Strategies, Functional Roles, and Clinical Translation for Tumor Treatment.

Photodynamic therapy (PDT) is an effective approach for inducing tumor cell death through reactive oxygen species (ROS) generated by light-activated photosensitizers (PSs). Despite its selectivity in tumor treatment, PDT still faces significant challenges in targeting deep-seated tumors due to limitations in tissue penetration and precise localization. Graphene-based nanomaterials, such as graphene oxide (GO), reduced graphene oxide (rGO), graphene quantum dots (GQDs), and graphene nanosheets (GNS), offer innovative solutions by enhancing light penetration, boosting PS activity, and improving tumor-targeting precision.

Temporal and Spatial Trends in the Global Burden of Acne Vulgaris Among Women of Reproductive Age: A Comprehensive Analysis and Forecast From 1990 to 2040.

Background: Acne is a common, multifactorial skin condition with significant psychosocial and public health impacts. However, its burden among reproductive-age women remains underexplored.

Methods: Using Global Burden of Disease (GBD) 2021 data, we analyzed acne incidence, prevalence, and disability-adjusted life years (DALYs) in females aged 15-49 across 204 countries (1990-2021).

Differential Susceptibility to Porcine Deltacoronavirus: Ducks Show Greater Vulnerability Than Geese.

Porcine deltacoronavirus (PDCoV), an enteropathogenic coronavirus belonging to the genus, is globally distributed and represents a significant viral enteric disease in pigs. It primarily causes severe diarrhea in pigs, especially in newborn piglets, with high fatality rates, resulting in substantial losses to the pig farming industry. Notably, PDCoV is also recognized as a zoonotic virus.

Transcriptomic Signature-Guided Depletion of Intermediate Alveolar Epithelial Cells Ameliorates Pulmonary Fibrosis in Mice.

Single-cell RNA sequencing (scRNA-seq) analyses of human and murine models of pulmonary fibrosis have uncovered multiple novel cell states involved in lung repair and regeneration, including an intermediate epithelial cell state identified as the KRT5-/KRT17+ aberrant basaloid cells in humans and the cytokeratin 8-positive alveolar differentiation intermediate (Krt8+ ADI) in mice. However, the specific contributions of these transitional cells to fibrogenesis remain poorly understood. In this study, we introduce a novel RNA-sensing-dependent protein translation technology that enables selective targeting and functional interrogation of Krt8+ ADI cells both and .

Paving the way for rural revitalization: Empirical analysis of the 'Sihao Rural Road Policy' and transport mode choice in Inner Mongolia, China.

This study investigates the impact of the Sihao Rural Road (SHRR) Program on travel behavior in Qingshuihe County, Inner Mongolia, China focusing on travel mode choice, travel frequency, and travel distance. Using an integrated Structural Equation Model (SEM) and Discrete Choice Model (DCM) framework, data were collected from 127 households between August 2023 and March 2024. The analysis reveals that the SHRR program significantly reduces travel frequency, likely due to improved local accessibility that decreases the need for frequent trips.

Molecular features and cell composition of left-dominant arrhythmogenic cardiomyopathy reveals key pathways and therapeutic targets.

Background: Arrhythmogenic cardiomyopathy (ACM) is a myocardial disorder characterized by arrhythmias and an increased risk of sudden cardiac death, particularly in left-dominant arrhythmogenic cardiomyopathy (LACM), which primarily affects the left ventricle. This study aims to elucidate the cellular and molecular mechanisms underlying LACM by performing an in-depth single-nucleus RNA sequencing (snRNA-seq) analysis to identify key transcriptional signatures and pathways involved in the disease's pathogenesis.

Method: Human heart samples were collected from five patients undergoing heart transplantation due to ACM and from four healthy donors.

A nature-inspired metal-free electrocatalyst towards efficient electron transfer and robust cascade oxygen reduction for wastewater treatment.

The pressing demand for removing high-risk emerging contaminants from wastewater calls for tailored treatment strategies, for which heterogeneous electrocatalysis induced by cascade oxygen reduction reaction (ORR) holds considerable potential. This process, however, suffers from poor interfacial electron transfer and discounted performance in non-acidic conditions. Inspired by the electron respiration chain of cells, a metal-free, quinone-based catalyst (PBth-BQ) was innovatively designed and synthesized.

Dysfunctional attitudes, social support, and adolescent depression.

Adolescent depressive symptoms represent a significant public health concern, with negative life events and dysfunctional attitudes playing pivotal roles in their development. A cross-sectional study by Yu assessed the interplay between dysfunctional attitudes, social support, and depressive symptoms in 795 Chinese adolescents (49.9% male, mean age 15.

Optimizing Zanubrutinib Dosing in Patients: A PBPK-BO Model Approach to Drug-Drug Interactions and Patients with Hepatic Impairment.

This study aimed to develop a physiologically based pharmacokinetic and Bruton's tyrosine kinase (BTK) occupancy (PBPK-BO) model to evaluate the pharmacokinetics (PK) and BTK occupancy (BO) of zanubrutinib (ZAN), particularly in relation to drug-drug interactions (DDIs) involving cytochrome P450 3A4 (CYP3A4) modulators and for patients with hepatic impairment. Population PBPK-BO and DDI models for ZAN were constructed using physicochemical properties, pharmacokinetic data, BTK occupancy levels, and physiological parameters. The PBPK-BO model was validated against clinically measured PK, DDI, and BO data, demonstrating accurate predictions of ZAN's plasma concentration and the time-course profiles of BO.

Therapeutic potential of natural flavonoids in atherosclerosis through endothelium-protective mechanisms: An update.

Atherosclerosis and its associated cardiovascular complications remain significant global public health challenges, underscoring the urgent need for effective therapeutic strategies. Endothelial cells are critical for maintaining vascular health and homeostasis, and their dysfunction is a key contributor to the initiation and progression of atherosclerosis. Targeting endothelial dysfunction has, therefore, emerged as a promising approach for the prevention and management of atherosclerosis.

THBS2-producing matrix CAFs promote colorectal cancer progression and link to poor prognosis via the CD47-MAPK axis.

Cancer-associated fibroblasts (CAFs) display significant functional and molecular heterogeneity within the tumor microenvironment, playing diverse roles in cancer progression. Employing single-cell RNA sequencing data of colorectal cancer (CRC), we identified a subset of matrix CAFs (mCAFs) as a critical subtype that secretes THBS2, a molecule linked to advanced cancer stages and poor prognosis. Spatial transcriptomics and multiplex immunohistochemistry revealed clear spatial colocalization between THBS2-producing mCAFs and tumor cells.