Integrated transcriptomics and metabolomics confirms the oxidative stress mechanism of hypothermia-induced neuronal necroptosis.

Abnormal climate change seriously endangers the safety of outdoor work and life, often causing hypothermia-induced coma or death. As the underlying mechanism has not been fully elucidated, a targeted treatment for hypothermia-triggered neuronal injury and forensic pathology indicators of fatal hypothermia are lacking. Herein, we aimed to explore hypothermia-induced changes in gene expression and metabolite profiles of cerebral cortical tissues to elucidate the mechanism of hypothermia-promoted necroptosis of cerebral cortical neurons.

The mechanism of parvalbumin interneurons regulating glutamatergic neurons involvement in stress induced anxiety in the basolateral amygdala of male mice.

Modern life's fast-paced and the unexpected conditions contribute to escalating stress levels, often leading to anxiety disorders and posing significant challenges to physical and mental health. In judicial practice, the parties often suffer from anxiety disorder under the great stress. However, the precise mechanisms underlying stress-induced anxiety disorders remain incompletely understood.

Inhibition of Ferroptosis Attenuates Neuron Damage and Improves Cognitive Impairment in Mice Surviving Severe Hypothermia.

Survivors of severe hypothermia frequently exhibit cognitive impairments. However, the underlying mechanisms remain inadequately understood. In order to reveal the scientific problem of cognitive dysfunction caused by severe hypothermia, providing an experimental basis for clinical treatment, this study utilized animal models and combined cognitive behavioral, morphological, and molecular biological experiments.

Research trends and hotspots of central sleep apnea: a bibliometric analysis.

Central sleep apnea (CSA), characterized by unstable ventilatory control during sleep, poses significant health risks, particularly in patients with cardiovascular comorbidities. This bibliometric analysis evaluated 1,687 CSA-related publications (2004-2025) from the Web of Science Core Collection. Annual publications surged post-2016, peaking in 2021 (115 articles), reflecting growing research interest.

Research status and hotspots of hypothermia and human diseases: a bibliometric analysis.

Background: Hypothermia has been strongly associated with human diseases; it affects life safety. Therapeutic hypothermia generates good results for certain diseases, without serious complications. In clinical practice, research on the treatment of hypothermia and severe hypothermia-induced diseases have achieved fruitful results.

Robot-assisted PFNA surgery improves clinical outcomes in the treatment of unstable femoral intertrochanteric fractures in elderly patients compared with traditional PFNA surgery.

To assess the clinical outcomes of robot-assisted proximal femoral nail antirotation (PFNA) surgery in elderly patients with unstable femoral intertrochanteric fractures (UFIFs). 151 patients who underwent UFIF and PFNA surgery between January 2020 and May 2024 were analyzed retrospectively. Of these, 78 patients were treated with traditional PFNA surgery (control group), and 73 patients were treated with robot-assisted PFNA surgery (observation group).

Application of a newly constructed NGS panel with 45 X-linked microhaplotypes demonstrates the unique value of X-MH for kinship testing and mixture analysis.

X-linked microhaplotypes (X-MHs) have the potential to be a valuable supplementary tool in complex kinship identification or the resolution of DNA mixtures, because they bring together the distinctive genetic pattern of X chromosomal markers and the benefits of microhaplotypes (MHs). In this study, we used the 1000 Genome database to screen and select 63 X-MHs; 18 MHs were filtered out though a batch sequencing assessment of the DNA samples collected from 112 unrelated Chinese Han individuals. The resulting 45-plex panel performed well in comprehensive assessments including repeatability, sensitivity, species specificity, resistance to PCR inhibitors or degradation, mutation rate, and accuracy in detecting DNA mixture samples.

The association between DNA methylation and human height and a prospective model of DNA methylation-based height prediction.

As a vital anthropometric characteristic, human height information not only helps to understand overall developmental status and genetic risk factors, but is also important for forensic DNA phenotyping. We utilized linear regression analysis to test the association between each CpG probe and the height phenotype. Next, we designed a methylation sequencing panel targeting 959 CpGs and subsequent height inference models were constructed for the Chinese population.

Differences of circular RNA expression profiles between monozygotic twins' blood, with the forensic application in bloodstain and saliva.

Monozygotic twins (MZTs) possess identical genomic DNA sequences and are usually indistinguishable through routine forensic DNA typing methods, which can be relevant in criminal and paternity cases. Recently, novel epigenetic methods involving DNA methylation and microRNA analysis have been introduced to differentiate MZTs. In this study, we explore the potential of using epigenetic markers, specifically circular RNAs (circRNAs), a type of non-coding RNA (ncRNA), to identify MZTs, and investigate the unique expression patterns of circRNAs within pairs of MZTs, enabling effective differentiation.

Feature selection with a genetic algorithm can help improve the distinguishing power of microbiota information in monozygotic twins' identification.

Introduction: Personal identification of monozygotic twins (MZT) has been challenging in forensic genetics. Previous research has demonstrated that microbial markers have potential value due to their specificity and long-term stability. However, those studies would use the complete information of detected microbial communities, and low-value species would limit the performance of previous models.

Development and evaluation of a novel panel containing 188 microhaplotypes for 2nd-degree kinship testing in the Hebei Han population.

Distant kinship identification is one of the critical problems in forensic genetics. As a new type of genetic marker defined and discussed in the last decade, the microhaplotype (MH) has drawn much attention in such identification owing to its specific advantages to traditional short tandem repeat (STR) or single nucleotide polymorphism (SNP) markers. In this study, MH markers were screened step by step from the 1000 Genomes Project database, and a novel multiplex panel containing 188 MHs (in which 181 are reported the first time, while 1 was reported in a previous study and the other 6 have partial overlaps with known markers) was constructed for application in 2nd- and 3rd-degree kinship identification.

A study of strong nucleosomes in the human genome.

Micrococcal nuclease (MNase) is widely used to map nucleosomes. However, nucleosomes are highly dynamic and susceptible to experimental conditions, resulting in extreme variability across nucleosome maps, which complicates the generation of accurate nucleosome organization data. We mapped nucleosomes from different individuals using improved MNase-seq.

Response Surface of Speed-Loading Path to Grain Refinement during Current-Heating Compression at SAE 5137H Steel.

In thermal deformation of materials, grain refinement induced by dynamic recrystallization (DRX) is often pursued to obtain excellent mechanical properties. Here, the thermal deformation behaviors of SAE 5137H steel were investigated and characterized at temperature and strain rate range of 1123-1483 K and 0.01-10 s.

Circular RNA as a Potential Biomarker for Forensic Age Prediction.

In forensic science, accurate estimation of the age of a victim or suspect can facilitate the investigators to narrow a search and aid in solving a crime. Aging is a complex process associated with various molecular regulations on DNA or RNA levels. Recent studies have shown that circular RNAs (circRNAs) upregulate globally during aging in multiple organisms such as mice and because of their ability to resist degradation by exoribonucleases.

Highly accurate mtGenome haplotypes from long-read SMRT sequencing can distinguish between monozygotic twins.

Discriminating between monozygotic twins (MZ) remains a challenge in the field of forensics globally. It is very difficult to find sequence variants within MZ twins, despite using ultra-deep next generation sequencing (NGS) for nuclear DNA. However, mitochondrial DNA might be a potential marker owing to its higher mutation rate and easier sequencing via NGS.

Development of an NGS panel containing 42 autosomal STR loci and the evaluation focusing on secondary kinship analysis.

High-throughput next-generation sequencing (NGS) is a feasible technique to detect considerably more markers and simultaneously obtain length and sequence information in a single reaction. In this study, we developed an NGS panel including 42 commonly used autosomal short tandem repeats (STRs) and amelogenin on the Illumina MiSeq FGx™. Sequencing accuracy was validated by the consistency of 2800M Control DNA detected using the ForenSeq™ DNA Signature Prep Kit and Sanger sequencing.

Uncovering the relationship and mechanisms of Tartary buckwheat () and Type II diabetes, hypertension, and hyperlipidemia using a network pharmacology approach.

Background: Tartary buckwheat (TB), a crop rich in protein, dietary fiber, and flavonoids, has been reported to have an effect on Type II diabetes (T2D), hypertension (HT), and hyperlipidemia (HL). However, limited information is available about the relationship between Tartary buckwheat and these three diseases. The mechanisms of how TB impacts these diseases are still unclear.

The complete chloroplast genome sequence of Chrysanthemum indicum.

Chrysanthemum indicum, an important medicinal plant of Asteraceae, had a long history in use for medicine in China. In this study, the complete chloroplast genome of C. indicum was sequenced by a 454 sequencing platform, and the structure of the obtained chloroplast genome was also analyzed.

The complete chloroplast genome sequence of Fagopyrum cymosum.

Fagopyrum cymosum is a traditional medicinal plant. In this study, the complete chloroplast genome of Fagopyrum cymosum is presented. The total genome size is 160,546 bp in length, containing a pair of inverted repeats (IRs) of 32,598 bp, separated by large single copy (LSC) and small single copy (SSC) of 84,237 bp and 11,014 bp, respectively.

The complete chloroplast genome sequence of Safflower (Carthamus tinctorius L.).

Safflower (Carthamus tinctorius L.) is a traditional medical plants of Asia. In this study, the complete chloroplast genome of safflower was presented.

The complete chloroplast genome sequence of Perilla frutescens (L.).

Perilla frutescens (L.) is a traditionally medical herb of East Asia. The complete chloroplast genome of P.

The development dynamics of the maize root transcriptome responsive to heavy metal Pb pollution.

Lead (Pb), as a heavy metal element, has become the most important metal pollutant of the environment. With allocating a relatively higher proportion of its biomass in roots, maize could be a potential important model to study the phytoremediation of Pb-contaminated soil. Here we analyzed the maize root transcriptome of inbred lines 9782 under heavy metal lead (Pb) pollution, which was identified as a non-hyperaccumulator for Pb in roots.