Differential impact of commercial rootstocks on the physiological response of a common walnut scion to drought stress.

Walnut rootstocks are commonly used in California orchards to provide resistance to soil-borne pests and diseases. However, little information exists about the impact of commercial rootstock on the common scion's physiological response under drought. This is becoming increasingly important since walnuts are commonly cultivated in semi-arid regions where frequent and severe droughts require efficient water use.

Some Like It Hot: Differential Photosynthetic Performance and Recovery of English Walnut Accessions Under Emerging California Heat Waves.

Heat waves (HWs) pose a significant threat to California agriculture, with potential adverse effects on crop photosynthetic capacity, quality and yield, all of which contribute to significant economic loss. Lack of heat-resilient cultivars puts perennial crop production under severe threat due to increasing HW frequency, duration and intensity. Currently, available walnut cultivars are highly sensitive to abiotic stress, and germplasm collections provide potential solutions via genotypes native to varied climates.

Mapping of drought-induced changes in tissue characteristics across the leaf profile of Populus balsamifera.

Leaf architecture impacts gas diffusion, biochemical processes, and photosynthesis. For balsam poplar, a widespread North American species, the influence of water availability on leaf anatomy and subsequent photosynthetic performance remains unknown. To address this shortcoming, we characterized the anatomical changes across the leaf profile in three-dimensional space for saplings subjected to soil drying and rewatering using X-ray microcomputed tomography.

Unique root hydraulic and mechanical properties support the resilience of grapevines adapted to the Atacama Desert.

Cortical lacunae caused by drought, especially observed in hybrids originating from Vitis rupestris, disrupt the connection between roots and soil. Yet, the physiological processes behind lacuna formation during drought and its consistency across Vitis species remain unclear. Here, we used a root pressure probe to investigate fine root hydraulic and mechanical properties, in the arid-adapted R-65 and drought-susceptible 101-14Mgt cultivars.

A variety-specific analysis of climate change effects on California winegrapes.

California contains a broad geography over which climate conditions can be suitable for cultivating multiple varieties of winegrapes. However, climate change is projected to make winegrape cultivation more challenging across many of California's winegrowing regions. In order to understand the potential effects of climate change on winegrapes, this study models variety-specific phenology for six winegrape varieties and quantifies the change in phenology and viticulturally-important agroclimate metrics over 12 of California's American Viticultural Areas (AVAs) by the mid-21st century.

Grape cultivars adapted to hotter, drier growing regions exhibit greater photosynthesis in hot conditions despite less drought-resistant leaves.

Background And Aims: Many agricultural areas are expected to face hotter, drier conditions from climate change. Understanding the mechanisms that crops use to mitigate these stresses can guide breeding for more tolerant plant material. We tested relationships between traits, physiological function in hot conditions and historical climate associations to evaluate these mechanisms for winegrapes.

Drought-induced fiber water release and xylem embolism susceptibility of intact balsam poplar saplings.

Balsam poplar (Populus balsamifera L.) is a widespread tree species in North America with significant ecological and economic value. However, little is known about the susceptibility of saplings to drought-induced embolism and its link to water release from surrounding xylem fibers.

Anatomy of an agricultural antagonist: Feeding complex structure and function of three xylem sap-feeding insects illuminated with synchrotron-based 3D imaging.

Many insects feed on xylem or phloem sap of vascular plants. Although physical damage to the plant is minimal, the process of insect feeding can transmit lethal viruses and bacterial pathogens. Disparities between insect-mediated pathogen transmission efficiency have been identified among xylem sap-feeding insects; however, the mechanistic drivers of these trends are unclear.

A workflow for segmenting soil and plant X-ray computed tomography images with deep learning in Google's Colaboratory.

X-ray micro-computed tomography (X-ray μCT) has enabled the characterization of the properties and processes that take place in plants and soils at the micron scale. Despite the widespread use of this advanced technique, major limitations in both hardware and software limit the speed and accuracy of image processing and data analysis. Recent advances in machine learning, specifically the application of convolutional neural networks to image analysis, have enabled rapid and accurate segmentation of image data.

Application of a remote-sensing three-source energy balance model to improve evapotranspiration partitioning in vineyards.

Unlabelled: Improved accuracy of evapotranspiration (ET) estimation, including its partitioning between transpiration (T) and surface evaporation (E), is key to monitor agricultural water use in vineyards, especially to enhance water use efficiency in semi-arid regions such as California, USA. Remote-sensing methods have shown great utility in retrieving ET from surface energy balance models based on thermal infrared data. Notably, the two-source energy balance (TSEB) has been widely and robustly applied in numerous landscapes, including vineyards.

Effects of meteorological and land surface modeling uncertainty on errors in winegrape ET calculated with SIMS.

Unlabelled: Characterization of model errors is important when applying satellite-driven evapotranspiration (ET) models to water resource management problems. This study examines how uncertainty in meteorological forcing data and land surface modeling propagate through to errors in final ET data calculated using the Satellite Irrigation Management Support (SIMS) model, a computationally efficient ET model driven with satellite surface reflectance values. The model is applied to three instrumented winegrape vineyards over the 2017-2020 time period and the spatial and temporal variation in errors are analyzed.

Improving the spatiotemporal resolution of remotely sensed ET information for water management through Landsat, Sentinel-2, ECOSTRESS and VIIRS data fusion.

Robust information on consumptive water use (evapotranspiration, ET) derived from remote sensing can significantly benefit water decision-making in agriculture, informing irrigation schedules and water management plans over extended regions. To be of optimal utility for operational usage, these remote sensing ET data should be generated at the sub-field spatial resolution and daily-to-weekly timesteps commensurate with the scales of water management activities. However, current methods for field-scale ET retrieval based on thermal infrared (TIR) imaging, a valuable diagnostic of canopy stress and surface moisture status, are limited by the temporal revisit of available medium-resolution (100 m or finer) thermal satellite sensors.

Structural and functional leaf diversity lead to variability in photosynthetic capacity across a range of Juglans regia genotypes.

Similar to other cropping systems, few walnut cultivars are used as scion in commercial production. Germplasm collections can be used to diversify cultivar options and hold potential for improving crop productivity, disease resistance and stress tolerance. In this study, we explored the anatomical and biochemical bases of photosynthetic capacity and response to water stress in 11 Juglans regia accessions in the U.

Desiccation of the leaf mesophyll and its implications for CO diffusion and light processing.

Leaves balance CO and radiative absorption while maintaining water transport to maximise photosynthesis. Related species with contrasting leaf anatomy can provide insights into inherent and stress-induced links between structure and function for commonly measured leaf traits for important crops. We used two walnut species with contrasting mesophyll anatomy to evaluate these integrated exchange processes under non-stressed and drought conditions using a combination of light microscopy, X-ray microCT, gas exchange, hydraulic conductance, and chlorophyll distribution profiles through leaves.

Development of High Performance Computing Tools for Estimation of High-Resolution Surface Energy Balance Products Using sUAS Information.

(small-Unmanned Aircraft System) and advanced surface energy balance models allow detailed assessment and monitoring (at plant scale) of different (agricultural, urban, and natural) environments. Significant progress has been made in the understanding and modeling of atmosphere-plant-soil interactions and numerical quantification of the internal processes at plant scale. Similarly, progress has been made in ground truth information comparison and validation models.

Evapotranspiration partitioning assessment using a machine-learning-based leaf area index and the two-source energy balance model with sUAV information.

Accurate quantification of the partitioning of evapotranspiration (ET) into transpiration and evaporation fluxes is necessary to understanding ecosystem interactions among carbon, water, and energy flux components. ET partitioning can also support the description of atmosphere and land interactions and provide unique insights into vegetation water status. Previous studies have identified leaf area index (LAI) estimation as a key descriptor of biomass conditions needed for the estimation of transpiration and evaporation.

Ecologically driven selection of nonstructural carbohydrate storage in oak trees.

Leaf habit is a major axis of plant diversity that has consequences for carbon balance since the leaf is the primary site of photosynthesis. Nonstructural carbohydrates (NSCs) produced by photosynthesis can be allocated to storage and serve as a resiliency mechanism to future abiotic and biotic stress. However, how leaf habit affects NSC storage in an evolutionary context has not been shown.

Inherent and Stress-Induced Responses of Fine Root Morphology and Anatomy in Commercial Grapevine Rootstocks with Contrasting Drought Resistance.

Some grapevine rootstocks perform better than others during and after drought events, yet it is not clear how inherent and stress-induced differences in root morphology and anatomy along the length of fine roots are involved in these responses. Using a variety of growing conditions and plant materials, we observed significant differences in root diameter, specific root length (SRL) and root diameter distribution between two commonly used commercial grapevine rootstocks: Richter 110 (110R; drought resistant) and Millardet et de Grasset 101-14 (101-14Mgt; drought sensitive). The 110R consistently showed greater root diameters with smaller SRL and proportion of root length comprised of fine lateral roots.

Maximum CO diffusion inside leaves is limited by the scaling of cell size and genome size.

Maintaining high rates of photosynthesis in leaves requires efficient movement of CO from the atmosphere to the mesophyll cells inside the leaf where CO is converted into sugar. CO diffusion inside the leaf depends directly on the structure of the mesophyll cells and their surrounding airspace, which have been difficult to characterize because of their inherently three-dimensional organization. Yet faster CO diffusion inside the leaf was probably critical in elevating rates of photosynthesis that occurred among angiosperm lineages.

Xylem network connectivity and embolism spread in grapevine(Vitis vinifera L.).

Xylem networks are vulnerable to the formation and spread of gas embolisms that reduce water transport. Embolisms spread through interconduit pits, but the three-dimensional (3D) complexity and scale of xylem networks means that the functional implications of intervessel connections are not well understood. Here, xylem networks of grapevine (Vitis vinifera L.

Modification of a gas exchange system to measure active and passive chlorophyll fluorescence simultaneously under field conditions.

Solar-induced fluorescence (SIF) is a promising tool to estimate photosynthesis across scales; however, there has been limited research done at the leaf level to investigate the relationship between SIF and photosynthesis. To help bridge this gap, a LI-COR LI-6800 gas exchange instrument was modified with a visible-near-infrared (VIS-NIR) spectrometer to measure active and passive fluorescence simultaneously. The system was adapted by drilling a hole into the bottom plate of the leaf chamber and inserting a fibre-optic to measure passive steady-state fluorescence ( , , analogous to SIF) from the abaxial surface of a leaf.

Modeling vegetative vigour in grapevine: unraveling underlying mechanisms.

Mechanistic modeling constitutes a powerful tool to unravel complex biological phenomena. This study describes the construction of a mechanistic, dynamic model for grapevine plant growth and canopy biomass (vigor). To parametrize and validate the model, the progeny from a cross of Ramsey () × Riparia Gloire () was evaluated.

Coordinated decline of leaf hydraulic and stomatal conductances under drought is not linked to leaf xylem embolism for different grapevine cultivars.

Drought decreases water transport capacity of leaves and limits gas exchange, which involves reduced leaf leaf hydraulic conductance (Kleaf) in both the xylem and outside-xylem pathways. Some literature suggests that grapevines are hyper-susceptible to drought-induced xylem embolism. We combined Kleaf and gas exchange measurements, micro-computed tomography of intact leaves, and spatially explicit modeling of the outside-xylem pathways to evaluate the role of vein embolism and Kleaf in the responses of two different grapevine cultivars to drought.

Xylella fastidiosa causes transcriptional shifts that precede tylose formation and starch depletion in xylem.

Pierce's disease (PD) in grapevine (Vitis vinifera) is caused by the bacterial pathogen Xylella fastidiosa. X. fastidiosa is limited to the xylem tissue and following infection induces extensive plant-derived xylem blockages, primarily in the form of tyloses.

Predicting Stomatal Closure and Turgor Loss in Woody Plants Using Predawn and Midday Water Potential.

Knowledge about physiological stress thresholds provides crucial information about plant performance and survival under drought. In this study, we report on the triphasic nature of the relationship between plant water potential (Ψ) at predawn and midday and describe a method that predicts Ψ at stomatal closure and turgor loss exclusively from this water potential curve (WP curve). The method is based on a piecewise linear regression model that was developed to predict the boundaries (termed Θ and Θ) separating the three phases of the curve and corresponding slope values.