Phosphatidylinositol 3-phosphate metabolism impacts cellular α-Synuclein localisation in Saccharomyces cerevisiae.

Alpha-Synuclein (αSyn), a hallmark protein of synucleinopathies such as Parkinson's disease, is likely to be involved in neuronal membrane trafficking and synaptic vesicle dynamics at axon terminals. Its specific binding to anionic phospholipids, such as phosphatidylinositol phosphates (PIPs) that are essential for intracellular signaling and membrane trafficking, suggests an involvement in vesicular transport processes. In Saccharomyces cerevisiae, a model organism for cell biological PD research, human αSyn localises to the plasma membrane via the secretory machinery.

Combination Therapy with Trehalose and Hyaluronic Acid Restores Tear Lipid Layer Functionality by Ameliorating Inflammatory Response Protein Markers on the Ocular Surface of Dry Eye Patients.

Topical lubricants are the fundamental treatment for dry eye disease (DED). However, the molecular mechanisms underlying their efficacy remain unknown. Here, the protective effects of Thealoz Duo with 3% trehalose and 0.

Molecular connectivity studies in neurotransmission: a scoping review.

Positron emission tomography (PET) and single photon emission computed tomography (SPECT) are essential molecular imaging tools for the in vivo investigation of neurotransmission. Traditionally, PET and SPECT images are analysed in a univariate manner, testing for changes in radiotracer binding in regions or voxels of interest independently of each other. Over the past decade, there has been an increasing interest in the so-calledapproach that captures relationships of molecular imaging measures in different brain regions.

Anterior-only decompression insufficient in concentric stenosis in degenerative cervical myelopathy.

Aims & Objectives: The compression of the spinal cord can result in degenerative cervical myelopathy (DCM), a condition characterized by progressive neurological dysfunction.This study examines clinical outcomes after anterior decompression and predictors of secondary posterior decompression after initial anterior surgery.

Materials & Methods: We conducted a retrospective review of 94 consecutive patients who underwent anterior-only cervical decompression in DCM.

Intraocular Pressure Fluctuations Recorded by a Telemetric Sensor After Nonpenetrating Glaucoma Surgery in Primary Open-Angle Glaucoma.

Objective: To investigate short- and long-term intraocular pressure (IOP) fluctuations in patients with primary open-angle glaucoma (POAG) after successful nonpenetrating glaucoma surgery (NPGS).

Design: Prospective, open-label, multicenter interventional study.

Subjects: A total of 20 patients with POAG who underwent NPGS combined with permanent implantation of a suprachoroidal telemetric IOP sensor (EyeMate-SC, Implandata Ophthalmic Products GmbH, Hanover, Germany).

Can open source large language models be used for tumor documentation in Germany?-An evaluation on urological doctors' notes.

Background: Tumor documentation in Germany is currently a largely manual process. It involves reading the textual patient documentation and filling in forms in dedicated databases to obtain structured data. Advances in information extraction techniques that build on large language models (LLMs) could have the potential for enhancing the efficiency and reliability of this process.

Sequelae of preterm birth over the lifespan: an exploratory analysis of behavioral problems in childhood and increased risk of major depression and anxiety in adulthood from a cohort study.

Background: Existing research has shown that prematurity and low birth weight are associated with mental health in children and adolescents, but their impact on mental health in adulthood and the mechanisms involved have been less studied. This study investigated the impact of prematurity and abnormal fetal growth, as a marker for fetal growth restriction, on mental health outcomes in adulthood.

Methods: This retrospective cohort study examined adults aged 18-52 years, categorized by gestational age (extremely preterm: ≤28 weeks; very preterm: 29-32 weeks; moderately preterm: 33-36 weeks; term: ≥37 weeks).

The role of interoceptive awareness in social anxiety disorder - results of an internet-based CBT-manualized therapist delivered group-treatment.

Background: Interoception, the process of detecting internal bodily signals, is linked to emotional regulation and mental health. Dysregulated interoceptive awareness (IAw) is associated with psychopathology, including social anxiety disorder (SAD), where internal cues influence negative self-perception and anxiety. While internet-based cognitive behavioral therapy (ICBT) offers an effective and more accessible treatment option, experimental treatment outcomes related to IAw and specific to SAD are rare.

Long-term prediction of blood pressure reduction after renal denervation for arterial hypertension.

Background: Renal denervation (RDN) has emerged as a potential therapy for lowering blood pressure (BP) in patients with arterial hypertension. However, approximately one-third of patients do not experience significant BP reductions, underscoring the need for reliable predictors of treatment response.

Objectives: This study evaluated the accuracy of a previously established bivariate prediction model for prediction of 3 months BP outcomes (based on baseline 24 h BP and ascending aortic distensibility) in predicting 24 h ambulatory BP outcomes up to 12 months post-RDN.

Paediatric acute liver failure: A prospective, nationwide, population-based surveillance study in Germany.

Objectives: Paediatric acute liver failure (PALF) is a rare but life-threatening condition, yet comprehensive epidemiological data in Germany are lacking. Our study aimed to systematically analyse incidence, aetiology, and outcome of PALF in Germany.

Methods: In a nationwide, population-based surveillance study, cases of PALF (defined following the PALF study group inclusion criteria) were queried from 2016 to 2018 through the German Paediatric Surveillance Unit (ESPED).

Kinetics of cell-free DNA in response to exogenous and exertional heating in healthy young men.

Purpose: Circulating cell-free DNA (cfDNA) is recognized as a relevant biomarker for monitoring the effects of various physiological and pathophysiological factors. To identify the effect of body temperature, we investigated the kinetics of cfDNA in response to two forms of heating.

Methods: In a randomized crossover design, twelve healthy young males cycled at 60% of their maximum oxygen uptake (VO) (exertional heating) and conducted lower-body immersion in hot water (43-44°C) (exogenous heating) until their rectal temperature (T) reached 39°C.

Acute appendicitis in patients suffering from Immunocompromising hematologic malignancies or chemotherapy-related leukopenia: a cohort study.

Background: Acute appendicitis is a common surgical emergency, but its diagnosis can be challenging in patients with hematologic malignancies or chemotherapy-induced leukopenia due to an impaired inflammatory response. The clinical presentation of acute appendicitis in these patients is often atypical, leading to delayed or misdiagnosis. This study aims to evaluate the outcomes of appendectomy in patients with hematologic malignancies or chemotherapy-induced leukopenia and assess the applicability of clinical scoring systems for the diagnosis of an acute appendicitis.

2025 ESVM Guidelines on interventional treatment of venous thromboembolism.

The number of endovascular interventional procedures for catheter-based therapy (CBT) of acute venous thromboembolism (VTE), comprising deep vein thrombosis (DVT) and pulmonary embolism (PE), has been increasing over the past years. The development of more efficient thrombectomy systems for CBT of VTE has potentially enhanced the efficacy of interventional treatment of VTE. Nevertheless, indications for CBT of VTE, i.

Baseline characteristics of patients with acute ischaemic stroke included in the randomised controlled Find-AF 2 trial.

Background: In the Find-AF 2 randomised controlled trial, we investigate whether a risk-adapted intensified heart rhythm monitoring with subsequent initiation of oral anticoagulation in ischaemic stroke patients leads to a reduction of recurrent ischaemic stroke and systemic embolism. The objective of this analysis is to present baseline characteristics of the overall Find-AF 2 study population and stratified by low or high risk for developing AF.

Methods: The Find-AF 2 trial included acute ischaemic stroke patients ≥ 60 years of age within 30 days of ischaemic stroke of any cause.

The RING Finger Protein RNF1 Forms an Interaction Network with Regulators of Sexual Development.

RNA-binding E3 ubiquitin ligases (RBULs) provide a link between RNA metabolic processes and the ubiquitin proteasome system (UPS). In humans, RBULs are involved in various biological processes, such as cell proliferation and differentiation, as well as sexual development. To date, little is known about their role in the protozoan parasite , the causative agent of malaria tropica.

Presynaptic maturation of inhibitory connections onto vasoactive intestinal polypeptide-expressing GABAergic interneurons in the mouse barrel field.

Vasoactive intestinal polypeptide-expressing inhibitory interneurons (VIP-INs) in the adult barrel cortex are crucial for mediating active whisking (AW) by disinhibiting pyramidal neurons. Past studies have investigated the development of VIP-IN network integration, focusing mainly on the excitatory network or the postsynaptic side of the inhibitory network. Hence, we aimed to explore the inhibitory network integration of VIP-INs, concentrating on the presynaptic side.

Ultrasound Guided Versus Conventional Closure Device Deployment Following Transfemoral Endovascular Procedures: A Systematic Review and Meta-analysis.

Objective: Femoral access site complications influence short term survival and outcomes in patients undergoing endovascular procedures. While ultrasound guided puncture is a reliable method to reduce such complications, ultrasound guidance is rarely used for closure device deployment.

Data Sources: Web of Science, PubMed, and the Cochrane Library.

The effect of an app-based health intervention on somatic symptoms among employees of the DHL Group: a longitudinal pilot study.

Background: Somatic symptoms significantly contribute to absenteeism and healthcare costs, particularly in physically demanding professions such as postal and delivery services. The FC-Fit Challenge, an app-based workplace health intervention, aims to promote healthier lifestyles through personalized feedback, social interaction, and professional guidance, targeting lifestyle changes and reducing somatic symptoms.

Objectives: This study evaluated the effect of the FC-Fit Winterchallenge 2021/22 on somatic symptoms over time, considering sociodemographic and work-related differences, and identified predictors of somatic symptoms to inform workplace health strategies.

A Cross-Sectional Survey Assessing the Factors Influencing Dentists' Decisions on Post-Endodontic Prosthetic Crown Restoration.

Interdisciplinary decision-making significantly influences both the therapeutic potential and clinical outcomes, shaping clinical attitudes and management strategies. As the integration between endodontic and restorative-prosthetic considerations becomes increasingly prevalent, it is essential to understand how different dental specialists, particularly general dental practitioners, prosthodontists and endodontists, approach clinical decision-making and collaborate to optimize patient care. : This study aims to identify practice disparities in post-endodontic crown placement to inform national policy reforms, including standardised timing protocols and interdisciplinary referral criteria.

Microglial SIRT2 deficiency aggravates cognitive decline and amyloid pathology in Alzheimer's disease.

Sirtuin 2 (SIRT2), a NAD+-dependent deacetylase, has been implicated in aging and neurodegenerative diseases such as Alzheimer's disease (AD). While global SIRT2 inhibition has shown promise in reducing amyloid-beta pathology and cognitive deficits in different mouse models of AD, peripheral SIRT2 inhibition has been associated with adverse effects, such as increased inflammation. This suggests that targeted inhibition of specific cellular populations within the brain may represent a more precise and effective approach for the treatment of AD.

Real-world Efficacy, Safety, and the Impact of Spherical Equivalent on Intravitreal Anti-VEGF Therapy in Patients Affected by Myopic Choroidal Neovascularisation.

Purpose: To analyse real-world clinical outcomes and influence of the spherical equivalent (SE) on anti-vascular endothelial growth factor (VEGF) therapy in patients affected by myopic choroidal neovascularisation (mCNV).

Patients And Methods: A retrospective investigation was conducted on treatment-naïve mCNV patients receiving anti-VEGF therapy at the Department of Ophthalmology, University Medical Center Mainz, Germany, between January 2018 and March 2024. Safety, functional and morphological changes in visual acuity (VA), central retinal thickness (CRT), and central retinal volume (CRV) were evaluated over a two-year period.

Early transcriptional states of spermatogonia and marker expressions in the prepubertal human testis following chemotherapy-induced depletion.

Study Question: Which spermatogonial differentiation states are present in prepubertal testes under normal conditions and following chemotherapy-induced depletion of spermatogonia in paediatric patients with cancer?

Summary Answer: Single-cell transcriptomic analysis reveals that only undifferentiated spermatogonia are present in prepubertal boys, while differentiated states emerge during puberty, with reduced protein expression of advanced spermatogonial markers observed in younger patients, those treated with alkylating agents, or those with a diminished spermatogonial pool.

What Is Known Already: Paediatric oncology treatments often involve gonadotoxic therapies that can impair spermatogonial stem cells, increasing the risk of subfertility. While five distinct spermatogonial subpopulations have been identified in adult testes via single-cell RNA sequencing, their presence in prepubertal testes of childhood cancer patients remains to be confirmed through marker protein expression.

Ixazomib-lenalidomid-dexamethasone (IRd) in relapsed refractory multiple myeloma (RRMM)-multicenter real-world analysis from Germany and comparative review of the literature.

With the introduction of novel agents (proteasome inhibitors (PIs), immunomodulatory drugs (IMiDs), immuntherapeutics), the outcome of multiple myeloma (MM) patients has improved. The oral PI ixazomib was authorized in the EU in 11/2016 and is indicated in combination with lenalidomide and dexamethasone (IRd) for the treatment of patients with relapsed/refractory MM (RRMM). The study aim was to identify the typical real world (RW) RRMM patient population in Germany treated with IRd and to compare the outcome to prior IRd cohorts.

The effectiveness of e-learning in focused cardiac ultrasound training: a prospective controlled study.

Introduction: Focused Cardiac Ultrasound (FOCUS) is an essential tool for rapid cardiac assessment across various clinical subspecialties. Consequently, teaching foundational FOCUS skills is of critical importance. This study investigates the effectiveness of e-learning in imparting FOCUS skills.

Prevalence and classification of posterior semicircular canal dehiscence in HRCTs of the petrous part of the temporal bone : High-resolution computed tomography of posterior semicircular canal dehiscence in combination with conductive or sensorineural hearing loss.

Background And Purpose: The complex anatomy of the petrous part of the temporal bone (or petrous temporal bone) is difficult to understand and constitutes a challenge in the daily diagnostic routine even for experienced neuroradiologists. High-resolution computed tomography (HRCT) is an integral part of equipment-based diagnostics of the rare entity of posterior semicircular canal dehiscence (PSCD). PSCD is characterised by an absence of the bony cover of the posterior semicircular canal in the petrous temporal bone.