Cystic Echinococcosis and Taenia spp. Infections in Nigeria: A Systematic Review and Meta-Analysis of Prevalence and Geographical Distribution.

Cystic echinococcosis (CE) and Taenia spp. infections are major zoonotic helminthiases with substantial public health and economic burdens, particularly in endemic regions. Despite their classification as neglected tropical diseases (NTDs), these tapeworm infections remain understudied in Nigeria.

Oxygen Vacancy-Rich Amorphous MOF/Graphene Nanozymes With Self-Sustaining Catalytic Circuits for Drug-Resistant Infection Wound Healing.

Diabetic chronic wounds, driven by hyperglycemia-induced oxidative stress and multidrug-resistant bacterial infections, represent a highly challenging clinical issue. Existing therapies fall short in addressing the dual challenges of bacterial resistance and dysregulated wound microenvironments. Although metal-organic framework (MOF)-based nanozymes hold potential for catalytic antibacterial therapy, their clinical application is limited by insufficient active site exposure, structural instability of amorphous MOFs (aMOFs), and dependence on toxic exogenous HO.

The PP2A-2 holoenzyme orchestrates daughter cell emergence during cytokinesis in Toxoplasma gondii.

Toxoplasma gondii is a significant pathogen in both humans and animals, with disease progression driven by the rapid proliferation of its tachyzoite stage. In this study, we identify the PP2A-2 holoenzyme as a key regulator of daughter cell emergence during parasite division. This holoenzyme, likely composed of the regulatory subunit TgPR48 (PP2A-B2), the catalytic subunit PP2A-C2, and the scaffolding subunit PP2A-A2, is essential for proper cytokinesis.

Comprehensive molecular epidemiology of BVDV in yaks () in Qinghai, China: high prevalence and dominance of BVDV-1u.

Introduction: Bovine viral diarrhea virus (BVDV) is a major pathogen affecting livestock health in China. However, the current epidemiological status in yaks (), particularly in Qinghai Province, remains insufficiently understood.

Methods: In the present study, a comprehensive serological and molecular investigation of BVDV in yaks was conducted across broad geographic areas of eight administrative regions including Yushu, Guoluo, Huangnan, Hainan, Haidong, Haixi, Haibei, and Xining in Qinghai Province.

Targeted Synthesis and Development of Neotype Antiviral Agents with a Radical-Mediated Deoxyphosphoamination Strategy.

The persistent threat of emerging and re-emerging viral infections underscores the urgent need for the continuous development of next-generation antiviral agents. The deoxygenative functionalization of oxygen-containing compounds offers a powerful route to address this challenge. Herein, we report a radical-mediated deoxyphosphoamination of ketones to synthesize a new class of antiviral compounds, including -(2-(diphenylphosphoryl)-1-inden-3-yl)-,-diphenylphosphinic amide (IDPA) and its homologues.

Ecological presence and functional role of bacteriophages in fermented vegetables.

Fermented vegetables are widely favored by consumers for their distinctive flavors and nutritional value, with their quality attributes being closely associated with microbiome dynamics. Recent advances in high-throughput sequencing technologies have revealed abundant bacteriophage resources within the fermented vegetable microbiome. These viral components significantly influence fermentation processes and product characteristics by modulating microbial community structure and function.

A novel Toxoplasma gondii thioredoxin (TgTrx1) is important for parasite fitness and virulence.

The protozoan parasite Toxoplasma gondii relies on antioxidant proteins and systems to protect against the host's immune responses and to neutralize free radicals produced by its own metabolism. In this study, we identified and characterized a new thioredoxin protein, TgTrx1, which is mainly found in the cytoplasm of T. gondii tachyzoites and contains a conserved -CXXC- catalytic motif.

Helicobacter hepaticus promotes hepatic steatosis through CdtB-induced mitochondrial stress and lipid metabolism reprogramming.

Host-pathogen interaction influences many non-infectious diseases, including metabolic diseases. Helicobacter hepaticus (H. hepaticus) has been found in some metabolic dysfunction-associated steatotic liver disease (MASLD) patients, however, the causal link and underlying mechanisms remain unclear.

Toxoplasma gondii PPM3H regulates the parasite virulence and modulates host immune and inflammatory responses in mice.

Toxoplasma gondii is an obligate intracellular parasite that causes severe illness in infants infected during pregnancy and in immunocompromised individuals. This parasite manipulates host cells through effector proteins that promote its survival and replication. While the phosphatases in the PP2C family have been shown to regulate host immune responses and contribute to the virulence and pathogenicity of various pathogens, the specific biological functions of PPM3H in T.

African swine fever virus DEAD-box helicase D1133L promotes OGG1-driven incision of genomic 8-oxoG via HDAC5 deacetylation.

African swine fever virus (ASFV) infection induces oxidative stress and produces oxidative DNA damage bases, including 8-oxoguanine (8-oxoG). It is essential to promptly repair these damages to maintain genome stability. The enzyme 8-oxoguanine DNA glycosylase 1 (OGG1) initiates the base excision repair (BER) pathway by recognizing and incising 8-oxoG and commonly regulates multiple biological processes by interacting with host and viral proteins.

Lumpy skin disease virus LSDV001 protein positively regulates inflammatory response by promoting assembly of the TAK1-TAB2/3 complex.

Unlabelled: Lumpy skin disease (LSD) is an emerging transboundary viral disease of livestock caused by the lumpy skin disease virus (LSDV), which has caused substantial economic losses and negatively impacted veterinary public health. The underlying pathogenic mechanisms remain poorly understood, limiting the effective prevention and control of LSD. Here, we identify LSDV-encoded protein LSDV001 as a positive regulator of IL-1β- and TNFα-triggered signaling.

Anti-inflammatory agent 35 reduces ASFV replication by suppressing the nuclear translocation of p65.

African swine fever (ASF), caused by the African swine fever virus (ASFV), is a highly contagious infectious disease. To date, no safe and effective vaccine or therapeutic drug is available for ASFV. In this study, we identified Anti-inflammatory agent 35 (A35) as a negative regulator of ASFV replication.

Rapid visual detection of Moniezia spp. in sheep feces via Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) assay.

Monieziasis is a prevalent issue in small ruminant husbandry, primarily caused by Moniezia expansa and M. benedeni in China. There is a critical need for highly sensitive methods for disease surveillance and prevention.

Screening and Validation of Negative Regulators of the Type I Interferon Pathway.

Type I interferons （IFN-I） are key proteins in antiviral response and immunomodulation. Negative regulators avoid abnormal activation of the interferon pathway or overactivation of interferon-activating proteins through multiple mechanisms. Loss-of-function mutations in negative regulator genes lead to the development of a variety of type I interferonopathy.

AP2X-1 is a negative regulator of sexual commitment.

is a widespread protozoan with a complex life cycle, characterized by transitions between various hosts and developmental stages, each tailored to a specific niche within its host. However, the regulatory mechanisms governing these life cycle transitions are not well understood. In this study, we investigated the AP2 factor AP2X-1, which is expressed during the tachyzoite and bradyzoite stages but decreases in the mature merozoite stage.

Overexpression of NK4 gene in TU212 affects migratory activity in laryngeal squamous cell carcinoma.

Background: Abnormal activation of the hepatocyte growth factor (HGF) and c-mesenchymal-epithelial transition factor (c-Met) signaling pathway is associated with tumor occurrence and development. Serum HGF concentrations are significantly higher in patients with advanced and poorly differentiated laryngeal squamous cell carcinoma than those with early and highly differentiated disease. , a splice variant of HGF, can competitively bind to c-Met and acts as a specific antagonist of HGF.

Ultrasound-assisted fermentation by Lactiplantibacillus paraplantarum for the preparation of camel milk polysaccharide-protein composite nanoparticles.

Polysaccharide-protein composite nanoparticles (PPNPs) are extensively utilized in food, drug delivery, and antioxidant materials due to their excellent biocompatibility, functionality, and environmental sustainability. Camel milk is abundant in bioactive compounds such as lactoferrin, lysozyme, and immunoglobulins, while also displaying low allergenicity, which provides an inherent advantage for functional particle preparation. Its unique nutritional profile and limited availability make it a promising resource for developing highly valuable nanoparticles (NPs).

An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development.

Developing an efficient and safe vaccine for African swine fever (ASF), a devastating disease of pigs, remains a significant challenge mainly due to limited knowledge of the immune correlates of protection. Identifying protective determinants is difficult because ASF virus (ASFV) is a large and complex DNA virus encoding over 160 proteins. Here, we constructed an ASFV proteome microarray containing 160 full-length proteins for profiling ASFV-specific antibodies.

Transcriptional Analysis of Sf9 Cells Infected with Cypovirus-23.

cypovirus-23 (DnCPV-23) is a new type of cypovirus that has a lethal effect on many species of Sphingidae pests. DnCPV-23 can replicate in Sf9 cells, but the replication characteristics of the virus in this cell line are still unclear. To determine the replication characteristics of DnCPV-23 in Sf9 cells, uninfected Sf9 cells and Sf9 cells at 24 and 72 h after DnCPV-23 infection were collected for transcriptome analysis.

Live-attenuated Toxoplasma gondii PruΔpp2a-c mutant elicits protective immunity against toxoplasmosis in mice and cats.

Toxoplasma gondii is a zoonotic protozoan pathogen capable of infecting humans and nearly all warm-blooded animals, and causing substantial economic losses to the livestock industry. Developing an effective vaccine against T. gondii remains an urgent priority for controlling the spread of this zoonotic parasite.

Pathogen Profiling and Molecular Evaluation Along With In Silico Modeling of Voltage-Gated Sodium Channel Gene in Deltamethrin Exposed Haemaphysalis longicornis Ticks.

Haemaphysalis longicornis is associated with the transmission of 30 various types of pathogens, having medical and veterinary importance. Hence, this study was designed to investigate the prevailing pathogen in Zhangjiachuan county, Gansu province, China. For the said purpose, we performed a molecular investigation of Rickettsia and Piroplasms pathogens in morpho-molecularly confirmed H.

Visualization and tracking of tubule-derived, fluorescent-labeled NS1 as a marker of bluetongue virus in living cells.

Bluetongue virus (BTV) is an arbovirus (genus, ; family, ) transmitted among ruminant hosts by biting midges (genus, ). . produce large numbers of virus-specific tubules during infection.

[A truncated N protein-based ELISA method for the detection of antibodies against porcine deltacoronavirus].

This study aims to establish an antibody detection method for porcine deltacoronavirus (PDCoV). The recombinant proteins PDCoV-N1 and PDCoV-N2 were expressed via the prokaryotic plasmid pColdII harboring the N gene sequence of the PDCoV strain CH/XJYN/2016. The reactivity and specificity of PDCoV-N1 and PDCoV-N2 with anti-PEDV sera were analyzed after the recombinant proteins were analyzed by SDS-PAGE and purified by the Ni-NTA Superflow Cartridge.

[Development and evaluation of a competitive ELISA based on a porcine neutralizing Fab antibody against Senecavirus A].

Senecavirus A (SVA) is a major viral pathogen causing disease in pigs, and effective monitoring of SVA infection is critical for disease control. In this study, we aimed to develop a reliable ELISA method for rapidly detecting neutralizing antibodies against SVA. We used HEK293F cells to express an SVA-specific porcine Fab antibody and verified the biological activity of the Fab antibody by indirect ELISA, immunofluorescence assay, virus neutralization test, and Western blotting.

[Novel cecropin D-derived peptide with inhibitory effect on porcine reproductive and respiratory syndrome virus entry].

Porcine reproductive and respiratory syndrome (PRRS), caused by the porcine reproductive and respiratory syndrome virus (PRRSV), is one of the major diseases threatening the swine industry. This study aims to rationally design and optimize natural antimicrobial peptides to identify antiviral candidates with potent inhibitory activity against PRRSV, thereby establishing a foundation for the development of novel preventive and therapeutic agents targeting PRRS. In this study, with cecropin D (CD) as the parent peptide, three derivatives (CD-2, CD-3, and CD-4) were designed through amino acid substitutions.