Enhancing mortality prediction in AIDS and disseminated : the impact of novel inflammatory markers in a nomogram.

(TM) is a common opportunistic infection among patients with AIDS, characterized by rapid systemic dissemination and a high mortality rate. Early identification of patients at risk of death is critical to improving clinical outcomes. Despite the severity of disseminated TM infection (DPSM), few predictive tools exist to assess mortality risk in affected AIDS patients.

Global research trends on the relationship between gut microbiota and melanoma from 2014 to 2023: a bibliometric and visualization analysis.

Melanoma, a type of malignant tumor, originates from skin cells known as melanocytes. The gut microbiota is closely linked to melanoma, and this bibliometric analysis aims to guide future studies and advance research into the role of the gut microbiome in melanoma. We used the Web of Science Core Collection database to identify relevant literature on the function of intestinal flora in melanoma from 2014-2023, and bibliometric analysis and network visualization were performed with VOSviewer, CiteSpace.

Clinical, Microbiological, and Antibiotic Treatment Characteristics of Bacterial Infections in Patients with Liver Cirrhosis in China: A Multicenter Study.

Background And Aims: Epidemiological data on bacterial infections in cirrhosis in China remain limited. Therefore, we aimed to conduct a multicenter study to investigate the characteristics and outcomes of patients with cirrhosis and bacterial infections in China.

Methods: We retrospectively enrolled 1,438 hospitalized adult patients with cirrhosis and bacterial or fungal infections from 24 hospitals across China between January 2018 and September 2024.

Case Report: Multidisciplinary management of primary omental pregnancy involving intraoperative ultrasound.

Introduction: Primary omental pregnancy (OP) is very rare, and achieving an accurate diagnosis has always been a challenge for obstetricians, gynecologists, and sonographers. Our attempt to utilize intraoperative ultrasonography has facilitated a definitive diagnosis for masses lacking obvious purple or blue characteristics under observation. This approach could provide a solution for challenges faced in intraoperative diagnosis.

In silico analysis-aided pharmacochemistry strategy to explore the pharmacodynamic material basis and mechanism of traditional Chinese medicine: Qingfei Mixture as a model.

Background: Qingfei Mixture (QFM) is a traditional Chinese medicine (TCM) prescription that is clinically used as an adjuvant treatment for mid- and late-stage lung cancer. However, the pharmacodynamic material basis and underlying mechanism of QFM against non-small cell lung cancer (NSCLC) remain unclear.

Aim Of The Study: To develop an in silico analysis-aided pharmacochemistry strategy to explore the pharmacodynamic material basis and mechanism of TCM prescription, using QFM as a model example.

Single-cell multimodal analysis reveals the dynamic immunopathogenesis of HBV-ACLF progression.

Background: Acute-on-chronic liver failure (ACLF) is a life-threatening syndrome involving dysfunction of multiple immune cell types.

Objective: This study aimed to comprehensively depict the dynamic trajectory of immune responses throughout the disease course of HBV-related ACLF (HBV-ACLF).

Design: Single-cell RNA sequencing and single-cell proteomics were performed on the peripheral blood mononuclear cells of 45 samples from 17 patients who were hospitalised (progressive/stable/recovering course of HBV-ACLF, 6/5/6) and 15 control subjects (liver cirrhosis, chronic hepatitis B and healthy controls, 5/5/5).

Innovative Biomarkers for Diagnosing Malignant Ascites in Liver Cancer.

Background: Liver cancer ranks among the most prevalent and lethal malignancies worldwide, with metastatic malignant ascites being a common complication. This study seeks to assess the diagnostic significance of high fluorescent cells (HFCs), biochemical and tumor markers in predicting the development of metastatic malignant ascites in patients with liver cancer.

Methods: We collected ascites samples from 266 patients diagnosed with liver cancer.

Norwegian scabies coinfected with disseminated Talaromyces marneffei - a dual challenge in HIV immunodeficiency: A case report.

Rationale: Norwegian scabies (crusted scabies) is an extreme manifestation of Sarcoptes scabiei infestation, primarily affecting immunocompromised populations such as HIV-positive individuals. This condition is characterized by a severe mite burden and high transmissibility, often accompanied by pathognomonic hyperkeratotic plaques.

Patient Concerns: A 46-year-old male patient diagnosed with HIV presented with persistent fever and progressive cutaneous desquamation, along with lymphadenopathy and worsening rash, initially misdiagnosed as atopic dermatitis.

Prognostic Value of Peripheral Blood nCD64 Index, mHLA-DR, and CD14monocyte Percentage in Different Infection Status in COVID-19 Patients.

Objective: To explore the value of the neutrophil CD64 (nCD64) index, monocytic HLA-DR (mHLA-DR), and the percentage of CD14 monocytes in the prognosis of Coronavirus disease 2019 (COVID-19).

Methods: Fifty-seven COVID-19 patients from December 2022 to November 2023 were divided into two groups: non-severe group (mild or moderate cases) and severe group (severe or critical cases). Among them, 17 patients were deceased.

Multi-omics analysis uncovers predictive biomarkers for the efficacy and outcomes of chemoimmunotherapy in advanced unresectable biliary tract cancers.

Background: The limited response rates of immune checkpoint inhibitors in biliary tract cancers (BTC) highlight the need for effective biomarkers to optimize patient selection.

Methods: Baseline tumor tissues from 125 patients with advanced BTC treated with first-line chemoimmunotherapy (chemoIO) were analyzed using targeted DNA sequencing and bulk RNA sequencing. In vitro and in vivo experiments were conducted to investigate the role of identified biomarkers in BTC.

Coexisting tubal pregnancy and intrauterine pregnancy during a natural pregnancy, first diagnosed via ultrasonography: A case report.

Rationale: The coexistence of intrauterine and extrauterine pregnancies is termed heterotopic pregnancy (HP). Most reported cases of HP occur after assisted reproductive technology, and HP in a natural pregnancy (NP) is very rare, often discovered intraoperatively. HP is a significant challenge for obstetricians, gynecologists, and sonographers.

Diagnostic value of serum Golgi protein 73 in liver fibrosis and inflammation in patients with autoimmune hepatitis.

There is a lack of reliable serum markers to reflect the fibrosis stage and necroinflammation grade in autoimmune hepatitis (AIH) patients. In this study, we investigated the diagnostic potential of a noninvasive serum marker, Golgi protein 73 (GP73), for fibrosis and inflammation in AIH patients. Serum GP73 concentrations were measured in a retrospective cohort of 79 patients with AIH who underwent liver biopsy.

LC-MS/MS based cellular pharmacokinetics to explore the mechanism of palmatine against doxorubicin-induced cardiotoxicity.

Doxorubicin (DOX) is an effective chemotherapeutic agent, but its use is limited by dose-dependent cardiotoxicity. Palmatine (PAL) has shown potential in preventing doxorubicin-induced cardiotoxicity (DIC); however, the underlying mechanism remains unclear. Since DOX accumulation in cardiomyocytes is a key factor in the development of cardiotoxicity, this study aims to explore the pharmacokinetic mechanisms through which PAL alleviates DIC.

Validated target-based network pharmacology reveals that salvianolic acid C attenuates doxorubicin-induced cardiotoxicity by regulating apoptosis and oxidative stress.

Doxorubicin (DOX), an anthracycline commonly used to treat various cancers, is associated with irreversible and dose-dependent cardiotoxicity. Salvianolic acid C (SAC) is a major polyphenolic compound in Salvia miltiorrhiza, an herbal medicine with significant cardioprotective effects. However, the role and mechanism of SAC in protecting against DIC remain unreported.

Extracellular vesicles as a promising platform of precision medicine in liver cancer.

Extracellular vesicles (EVs) are natural carriers of biological information and play pivotal roles in intercellular communication. EVs are biocompatible, have low immunogenicity, and are capable of traversing biological barriers, making them ideal tools for disease diagnosis and therapy. Despite their promising prospects, the full realization of EVs potential faces several challenges.

Host factor RBM25 promotes HBV replication through Yin Yang 1-mediated cccDNA transcription.

The persistence of covalently closed circular DNA (cccDNA) in hepatitis B virus (HBV)-infected hepatocytes remains a major obstacle to effective antiviral treatment. Understanding the molecular mechanisms regulating HBV cccDNA transcription is essential for developing novel therapeutic strategies. In this study, we investigated the role of RNA binding motif protein 25 (RBM25) in HBV replication, focusing on its interaction with cccDNA and its regulation of host transcription factors.

Prediction models of breast cancer molecular subtypes based on multimodal ultrasound and clinical features.

Background And Aims: Breast cancer classify into four molecular subtypes: Luminal A, Luminal B, HER2-overexpressing (HER2), and triple-negative (TNBC) based on immunohistochemical assessments. The multimodal ultrasound features correlate with biological biomarkers and molecular subtypes, facilitating personalized, precision-guided treatment strategies for patients. In this study, we aimed to explore the differences of multimodal ultrasound features generated from conventional ultrasound (CUS), shear wave elastography (SWE) and contrast-enhanced ultrasound (CEUS) between molecular subtypes of breast cancer, investigate the value of prediction model of breast cancer molecular subtypes based on multimodal ultrasound and clinical features.

Revealing roles of PANoptosis-related genes in prognosis and molecular subtypes in lung squamous cell carcinoma by integrated bioinformatic analyses and experiments.

The purpose of current study was to reveal the role of PANoptosis-associated genes in lung squamous cell carcinoma (LUSC) and their potential as prognostic biomarkers. We analyzed RNA-seq data from TCGA-LUSC and GEO datasets to identify differentially expressed genes (DEGs) between LUSC and normal samples, followed by VENN analysis to reveal PANoptosis-related DEGs. Functional enrichment analyses were performed by clusterProfiler package.

LINC01711 modulates proliferation, migration, and extracellular matrix deposition of hypertrophic scar fibroblasts by targeting miR-34a-5p.

Hypertrophic scar (HS) is a proliferative disorder that occurs after skin injury and generally leads to disfigurement and impaired skin function in patients. This study aims to delve into the biological role of long intergenic non-protein coding RNA 1711 (LINC01711) in HS, thereby identifying novel therapeutic approaches for HS. HS tissues and corresponding normal tissues were obtained from 35 patients.

Diagnostic value of metagenomic next-generation sequencing in detecting pneumonia in HIV-infected patients.

Introduction: Accurate diagnosis of pneumonia (PJP) in HIV patients remains challenging. This study compares metagenomic next-generation sequencing (mNGS) with PCR, GMS staining, and serum β-D-glucan (BG) assays for PJP detection and co-infection identification.

Methods: BALF samples from 34 HIV-positive PJP patients and 50 non-PJP controls were analyzed.

Biochemical and structural insights into position 97 micropolymorphisms in human leukocyte antigen (HLA)-C*12 allotypes and their differential disease associations.

Micropolymorphisms drastically shape the antigen presentation characteristics of human leukocyte antigen class I (HLA-I) molecules, with profound implications for immune responses and disease susceptibility. HLA-C*12:02 and HLA-C*12:03 are closely related HLA-I allotypes that differ by a single amino acid substitution (R97W) but exhibit distinct associations with disease. HLA-C*12:02 has been shown to provide protective effects against HIV infection, playing a crucial role in controlling viral replication and slowing disease progression, whereas HLA-C*12:03 is associated with increased susceptibility to psoriasis.