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A novel probe DFC (2-(dicyanomethylene)-2,5-dihydro-5,5-dimethyl-4-(()-2-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)furan-2-yl)vinyl)furan-3-carbonitrile) was successfully designed and synthesized for the detection of l-lysine (l-Lys). The sensing behavior was characterized using absorption and fluorescence emission spectra. Upon addition of l-Lys to DFC, a rapid response time of 5 seconds was observed, accompanied by a significant 4-fold enhancement in fluorescence intensity. Additionally, DFC exhibits an impressively low detection limit of 0.14 μMol L. Furthermore, the applicability of DFC was demonstrated through successful detection of l-Lys in water samples, food samples, and imaging of l-lys in live HeLa cells.
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http://dx.doi.org/10.1039/d5ra00621j | DOI Listing |
Anal Chem
September 2025
Department of Laboratory Medicine, Fujian Medical University, Fuzhou 350004, China.
Acute lymphoblastic leukemia (ALL) is the most common hematologic malignancy in children. Current clinical diagnosis primarily relies on invasive detection methods, while molecular subtyping remains a complex and time-consuming process. This study innovatively employed silver nanoparticle-based surface-enhanced Raman spectroscopy (SERS) technology to systematically analyze 116 serum samples, including those with breakpoint cluster region-Abelson (-) fusion genotype, mixed-lineage leukemia (, also known as lysine methyltransferase 2A, ) gene rearrangement subtype, T-lymphoblastic ALL, and healthy controls.
View Article and Find Full Text PDFFront Pharmacol
August 2025
Department of Genetics and Cell Biology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, China.
Background: Osteoporosis (OP) is a chronic, systemic skeletal disorder characterized by progressive bone loss and microarchitectural deterioration, which increases fracture susceptibility and presents a challenging set of global healthcare problems. Current pharmacological interventions are limited by adverse effects, high costs, and insufficient long-term efficacy. Here, we identify snow crab shell-derived polypeptides (SCSP) as a potent osteoprotective agent.
View Article and Find Full Text PDFChemistry
September 2025
Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA, 02129, USA.
Nucleic acid-based therapeutics, such as oncolytic virotherapy or gene therapy, would benefit greatly from a reporter gene that induces endogenous production of a protein biomarker to noninvasively track the delivery, persistence, and spread with imaging. Several chemical exchange saturation transfer (CEST) reporter proteins detectable by magnetic resonance imaging (MRI) have been demonstrated to have high sensitivity. However, to date none can provide strong CEST contrast at a distinct resonance from that of endogenous proteins, limiting their specificity.
View Article and Find Full Text PDFZhonghua Yan Ke Za Zhi
September 2025
Department of Ophthalmology, The Third Xiangya Hospital, Central South University, Changsha 410013, China.
To explore the effects of aging on the stiffness of human scleral fibroblast (HSF) and the remodeling of the extracellular matrix. This experimental study was conducted from January 2022 to June 2024. HSFs were cultured, and after cell passage, β-galactosidase staining was conducted.
View Article and Find Full Text PDFJ Environ Pathol Toxicol Oncol
September 2025
Department of Clinical Laboratory Medicine, Fujian Medical University, Fuzhou, China.
Invasive ductal carcinoma (IDC) is a major type of breast cancer. The utilization of inhibitors targeting histone methyltransferases introduces novel therapeutic avenues for the treatment of cancer. Immunohistochemistry, Western blot, and reverse transcription quantitative polymerase chain reaction experiments were applied to assess the levels of EHMT2 in IDC and adjacent tissues.
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