Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
This study successfully obtained a chlorophyll-deficient mutant of Chlorella pyrenoidosa, named A19, using atmospheric and room temperature plasma mutagenesis technology. Compared to the wild-type strain, the A19 mutant exhibited a faster growth rate and appeared yellow under dark conditions. The content of chlorophyll A and chlorophyll B decreased by 80% and 60%, respectively, while lutein content increased by 16%. A19 also demonstrated improved amino acid quality. Transcriptome analysis revealed upregulation of gene expression levels in glycolysis and tricarboxylic acid cycle pathways, promoting central carbon flow towards enhanced production capacity and accelerating growth. Meanwhile, downregulation of genes regulating enzymes in the chlorophyll synthesis pathway explained the observed color change in the mutant strain. These results confirmed that A19 not only shifts its color to yellow but also exhibits faster growth, higher lutein content, and improved metabolic efficiency, making it a promising additive for feed and food applications while reducing cultivation time.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biortech.2025.132245 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Electrostatic and functional group-mediated biosorption of microalgae and lead using biosafe Aspergillus oryzae 3.042 fungal pellets.
Bioresour Technol
September 2025
College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China; Innovation Research Center for Advanced Environmental Technology, Eco-industrial Innovation Institute ZJUT, Quzhou 324400, PR China. Electronic address:
Co-occurring algal blooms and lead (Pb) pollution pose severe threts to freshwater ecosystems. In this study, Aspergillus oryzae (A. oryzae) 3.
View Article and Find Full Text PDFPhytohormone-augmented microalgae: A dual-functional solution for pharmaceutical contaminants removal and resource recovery.
J Hazard Mater
September 2025
School of Petrochemical Engineering & Environment, Zhejiang Ocean University, Zhoushan 316000, China; National & Local Joint Engineering Research Center of Harbor Oil & Gas Storage and Transportation Technology, Zhejiang Key Laboratory of Pollution Control for Port-Petrochemical Industry, Zhoushan 3
Microalgae-based wastewater treatment offers dual advantages of carbon sequestration and resource recovery, yet its efficacy in removing pharmaceutical contaminants (PCs) remains limited. Phytohormone augmentation presents a promising strategy to enhance PCs removal and bioresource production, but the mechanisms and scalability of this approach are poorly understood. This study systematically evaluated the dual effects of seven phytohormones including indole-3-acetic acid (IAA), abscisic Acid (ABA), salicylic acid (SA), gibberellin A3 (GA3), jasmonic acid (JA), brassinolide (BR), and zeatin (ZT) on the degradation of triclocarban (TCC) and the production of bioresources in Chlorella pyrenoidosa.
View Article and Find Full Text PDFData-driven machine learning modeling reveals the impact of micro/nanoplastics on microalgae and their key underlying mechanisms.
J Hazard Mater
September 2025
Université Claude Bernard Lyon 1, Laboratoire d'Ecologie Microbienne, UMR CNRS 5557, UMR INRAE 1418, Vet Agro Sup, UMR CNRS 5557, UMR INRAE 1418, Vet Agro Sup, Villeurbanne 69622, France; Department F. A. Forel for Environmental and Aquatic Sciences, Section of Earth and Environmental Sciences and
Micro- and nano-plastics (MNPs) pose a growing threat to freshwater microalgae, leading to water quality and biodiversity. Traditional experiments often encounter difficulties in terms of cost, time, and capturing complex interactions when exploring this critical issue. To overcome these limitations, we applied eight machine learning models to predict MNPs' effects on microalgae activity using literature data from the past decade.
View Article and Find Full Text PDFEffects of Intracellular Polysaccharides and Proteins of on Water Quality, Floc Formation, and Microbial Composition in a Biofloc System.
Microorganisms
July 2025
School of Life Science, Huzhou University, Huzhou 313002, China.
The use of (formerly ) and its intracellular substances (ISs) to promote biofloc development has been extensively studied. To identify the key components of the ISs of that drive biofloc formation, algal-extracted polysaccharides (AEPSs) and algal-extracted proteins (AEPTs) were isolated from the ISs. In this study, we established four groups: ISs, AEPSs, AEPTs, and tap water (TW, control), to investigate the effects of AEPSs and AEPTs on biofloc formation dynamics, water quality parameters, and microbial community composition.
View Article and Find Full Text PDFPolysaccharide CPP-3a Promotes M1 Polarization of Macrophages via TLR4/2-MyD88-NF-κB/p38 MAPK Signaling Pathways.
Mar Drugs
July 2025
Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine, Guangxi University of Chinese Medicine, Nanning 530200, China.
The immunomodulatory polysaccharide CPP-3a, purified from , was investigated for its effects on RAW264.7 macrophages and underlying mechanisms, revealing that CPP-3a significantly enhanced phagocytic capacity and nitric oxide production while upregulating pro-inflammatory cytokines TNF-α and IL-6 and elevating the co-stimulatory molecule CD86, collectively driving robust M1 polarization. Mechanistically, TLR4-, TLR2-specific inhibitors, and TLR4-knockout cells confirmed TLR4 as the primary receptor for CPP-3a, with TLR2 playing a secondary role in cytokine modulation.
View Article and Find Full Text PDF