Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
NLR inflammasomes recognize pathogen-associated molecular patterns (PAMPs), triggering Caspase-1 activation and leading to gasdermin D (GSDMD)-mediated pyroptosis, a crucial immune response in mammals. The functional GSDME-mediated pyroptosis has been reported in invertebrates, yet the existence of an NLR-Caspase-GSDME axis mediating pyroptosis signaling cascades remains unclear. In this study, we reported an NLRC4 homolog named ChNLRC4, a pattern recognition receptor from the oyster Crassostrea hongkongensis that is able to bind to LPS and Lys-type PGN through its LRR domain. ChNLRC4 interacted with ChCaspase-1 through CARD-CARD domain homotypic interactions and enhanced ChCaspase-1 activity. Additionally, overexpression of ChNLRC4 promoted ChCaspase-1-mediated cleavage of ChGSDME, leading to pyroptosis in HEK293T cells. Furthermore, knockdown of chnlrc4 resulted in a significant reduction in the death rate of hemocytes, immune infiltration of hemocytes, cilium shedding, and bacterial clearance. Collectively, this study provides insight into the role of NLR within the pyroptosis signaling pathway in oysters.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2025.139632 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
NEK9-mediated Wnt signalling repressor TLE3 rewires Docetaxel resistance in cancer cells by inducing pyroptosis.
Br J Cancer
September 2025
Institute of Life Sciences, Bhubaneswar, Odisha, India.
Background: Docetaxel is the most common chemotherapy regimen for several neoplasms, including advanced OSCC (Oral Squamous Cell Carcinoma). Unfortunately, chemoresistance leads to relapse and adverse disease outcomes.
Methods: We performed CRISPR-based kinome screening to identify potential players of Docetaxel resistance.
Delayed administration of Jiuwei Qianghuo Decoction combined with Zhuye Shigao Decoction mitigates the severity of influenza A virus infection by inhibiting cell death and alleviating the subsequent cytokine storm response.
J Ethnopharmacol
September 2025
Institute of Respiratory Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. Electronic address:
Ethnopharmacological Relevance: The high mortality rate associated with severe influenza partly results from delayed initiation of antiviral therapy and subsequent cytokine storms. Jiuwei Qianghuo Decoction combined with Zhuye Shigao Decoction (JZF) has been clinically prescribed to prevent the progression to a more severe illness in influenza treatment. However, the precise mode of action and active components have not yet been elucidated.
View Article and Find Full Text PDFMetabolic cell death in cancer: mechanisms and therapeutic potential.
Apoptosis
September 2025
School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
A defining hallmark of malignant tumours lies in their pronounced resistance to programmed cell death mechanisms. This intrinsic resilience enables cancer cells to circumvent physiological clearance, thereby sustaining unchecked proliferation and survival. Emerging research has revealed that metabolic dysregulation can precipitate a distinctive form of programmed cell death, termed metabolism-linked regulated cell death (RCD), establishing it as a novel paradigm of cellular self-elimination.
View Article and Find Full Text PDFSuperiority of ultrasound-extracted Flammulina velutipes polysaccharides over hot water-extracted ones in mitigating colonic cell pyroptosis: Discoveries into modulation of NLRP3 pathway.
Food Res Int
November 2025
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:
Flammulina velutipes is a major edible fungus with abundant yield and mature industrial production technology. Its main functional component, Flammulina velutipes polysaccharide, has huge development and utilization value. In light of the current uncertainty regarding the mechanisms by which Flammulina velutipes polysaccharides prevent colonic cell pyroptosis, the mechanisms of ultrasound-extracted Flammulina velutipes polysaccharide (FVPU2) in inhibiting colonic cell pyroptosis in mice were investigated, and compared with Flammulina velutipes polysaccharide extracted via hot water extraction (FVPH2).
View Article and Find Full Text PDFTongxinluo alleviates myocardial ischemia-reperfusion injury by inhibiting the pyroptosis of endothelial cells via the NLRP3/Caspase-1/GSDMD signaling pathway.
J Mol Histol
September 2025
Hebei Medical University, No. 361, Zhongshan East Road, Shijiazhuang, 050017, China.
Numerous people experiencing acute myocardial infarction are also experiencing myocardial ischemia-reperfusion injury (MIRI). Pyroptosis is a core mechanism in MIRI. Tongxinluo (TXL) has a significant protective effect on endothelial cell function.
View Article and Find Full Text PDF