Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
During the reproductive life, most primordial follicles (PFs) remain dormant for years or decades, while some are progressively activated for development. Misactivation of primordial follicles can cause ovarian diseases, for example, premature ovarian insufficiency (POI). Our results show that p300 expression increased with primordial follicle activation. Using a p300 inhibitor resulted in premature activation of primordial follicles in cultured mouse ovaries. Conversely, the ratio of primordial follicle activation was markedly decreased upon culturing with the p300 agonist. Furthermore, p300 regulated primordial follicle activation by inhibiting transcription in granulosa cells. In addition, this study was extended to potential clinical applications, showing that short-term treatment with a p300 inhibitor in vitro significantly increased primordial follicle activation in newborn mouse ovaries after the renal subcapsular transplantation in female NSG mice. Our results revealed that p300 controls the activation of primordial follicles in mammalian ovaries. In this study, our results show that p300 expression increases with primordial follicle activation. A p300 inhibitor results in premature activation of primordial follicles in cultured mouse ovaries. Conversely, the ratio of primordial follicle activation markedly decreases upon culturing with the p300 agonist. Furthermore, p300 regulates primordial follicle activation by inhibiting transcription in granulosa cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1152/ajpcell.00198.2024 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
GADD45A is Essential for Granulosa Cells Differentiation and Ovarian Reserve in Human and Mice.
J Cell Mol Med
September 2025
Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.
Diminished ovarian reserve (DOR) poses significant challenges in reproductive health, with emerging evidence implicating DNA damage repair pathways. While GADD45A is a critical regulator of DNA repair, cell cycle and apoptosis, its role in DOR pathogenesis remains unexplored. We employed transcriptome sequencing, qPCR and Western Blot analyses to compare GADD45A expression in granulosa cells (GCs) between DOR patients and controls.
View Article and Find Full Text PDFThe impact of maternal diet-induced obesity on offspring primordial oocyte mitochondria at birth and at weaning.
Mol Hum Reprod
September 2025
Department of Veterinary Sciences, Laboratory of Veterinary Physiology and Biochemistry, Gamete Research Centre, University of Antwerp, Antwerp, Belgium.
Maternal diet-induced obesity (DIO) may affect adult offspring oocyte quality due to mitochondrial dysfunction. Here, we investigated whether offspring of DIO mothers exhibit mitochondrial abnormalities in their primordial follicle oocytes (PFOs) already at birth, and if (further) alterations can be detected at weaning. Female Swiss mice were fed a control or obesogenic diet for 7 weeks before mating, and throughout pregnancy and lactation.
View Article and Find Full Text PDFExposure to diethyl phthalates impairs ovarian reserve and oocyte maturation in female mice.
Ecotoxicol Environ Saf
September 2025
School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China; Institute for biological therapy, Henan Academy of Innovations in Medical Science, Zhengzhou, Henan 451163, China. Electronic address:
Diethyl phthalates (DEP) are commonly used as a plasticizer and have been found to cause male reproductive defects and metabolic disease in mammals as a potential environmental endocrine disruptor. However, the effects and underlying mechanisms of DEP exposure on female follicle development and oocyte maturation were still unclear. In this study, we found that mice exposed to DEP had significantly reduced primordial follicles.
View Article and Find Full Text PDFAquaporin 2 is differentially expressed in granulosa cells of various stages of human follicles and is regulated by luteinizing hormone.
Front Cell Dev Biol
August 2025
Reproductive Medicine Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
Introduction: Several aquaporins (AQPs) are involved in the influx of water to form follicular fluid, and AQP2 may play a crucial role in follicular growth. However, the specific roles of Aquaporin (AQP) 2 and AQP6 in granulosa cells (GCs) during follicular fluid (FF) formation, as well as their relationship with gonadotropins (Gn), remain unclear.
Methods: Luteinized granulosa cells (LGCs) were isolated from follicles of different diameters.
Engineering modification of human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorates polycystic ovary syndrome by enhancing the ovarian environment and regulating follicular development.
Stem Cell Res Ther
September 2025
State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, Jiangsu, China.
Background: Polycystic Ovary Syndrome (PCOS), is a complex endocrine disorder affecting 6-21% of reproductive-aged women, characterized by chronic anovulation, hyperandrogenism, and polycystic ovarian morphology. Current clinical management relies on lifestyle modifications and symptom-targeted therapies due to the absence of curative interventions. In recent years, Laparoscopic ovarian drilling (LOD), a surgical procedure that induces controlled ovarian damage to stimulate primordial follicle activation and regulate follicular growth, has emerged as an established therapeutic intervention for infertility in PCOS.
View Article and Find Full Text PDF